Ricardo F Bidone, Carlos Afonso Nobre, Claudio Fernando Mahler, Francisco Ricardo A Bidone
{"title":"COP 30: Brazil's great opportunity.","authors":"Ricardo F Bidone, Carlos Afonso Nobre, Claudio Fernando Mahler, Francisco Ricardo A Bidone","doi":"10.1590/0001-3765202520250245","DOIUrl":"https://doi.org/10.1590/0001-3765202520250245","url":null,"abstract":"","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20250245"},"PeriodicalIF":1.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A closer look reveals the first records of Campsurus Eaton, 1868 (Ephemeroptera: Polymitarcyidae: Campsurinae) from Maranhão state, Northeast Brazil, including a new species.","authors":"Jaime DE L Gama Neto, Mahedy A B Passos","doi":"10.1590/0001-3765202520240967","DOIUrl":"10.1590/0001-3765202520240967","url":null,"abstract":"<p><p>The male of Campsurus barreirinhas sp. nov. is described and illustrated, representing the first records of Campsurus from Maranhão State, Northeast Brazil region. It is nearly related to C. litaninensis, both showing a unique genitalia, with the sternum IX extremely projected medially, pedestals bases distinctly separated in the middle, and pedestal rotated. The new species differentiates from C. litaninensis because the projection of IX sternum is rounded distally and the penes are stouter. This new species was collected along with Campsurus essequibo and Campsurus lucidus, representing the first records of these species in the Northeast Brazilian region.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240967"},"PeriodicalIF":1.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seidel F Dos Santos, Ianara Tamyres F Borges, Ana Carla Feio, Alberdan S Santos
{"title":"Systematic investigation of phenolic compounds distribution in Libidibia ferrea (ironwood) seedling, using metabolomics and histochemical techniques.","authors":"Seidel F Dos Santos, Ianara Tamyres F Borges, Ana Carla Feio, Alberdan S Santos","doi":"10.1590/0001-3765202520240635","DOIUrl":"10.1590/0001-3765202520240635","url":null,"abstract":"<p><p>Ironwood (Libidibia ferrea) is cultivated and widely used due to its medicinal properties attributed especially to phenolic compounds. In the seedling stage, different environmental conditions influence the synthesis, composition, and accumulation of bioactive compounds in different ways, mainly under water stress. Thus, given the wide distribution, this study aimed to analyze the occurrence and distribution of phenolic compounds in L. ferrea seedling for three different states/climate typologies: Pará/Af, Amazonas/Am, and Maranhão/Aw. Gas chromatography-mass spectrometry (GC-MS) and plantlet histochemistry were applied to determine the phenolic compounds distribution present in the extracts of leaflets and roots, respectively. Three different metabolic profiles were described, of which Pará/Af and Maranhão/Aw were similar, while Amazonas/Am showed quantitative differences in the composition. Among the main phenolic compounds were found gallic acid, ferulic acid, and caffeic acid, and presented different concentrations according to origin. The only phenolic compound found for all origins was gallic acid. Therefore, it was demonstrated that the chemical profile of phenolic compounds is conserved in L. ferrea, and significant variations were not observed, which ensures the use of this species regardless of origin.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240635"},"PeriodicalIF":1.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"2024 - an interesting year for the Annals of the Brazilian Academy of Sciences.","authors":"Alexander W A Kellner","doi":"10.1590/0001-376520259701","DOIUrl":"https://doi.org/10.1590/0001-376520259701","url":null,"abstract":"","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20259701"},"PeriodicalIF":1.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Larissa S Gualberto, Andréia Ibiapina, Bianca B Dias, Bárbara C B DE Freitas, Antônio A DE Melo Filho, Rômulo A Morais, Glêndara A S Martins
{"title":"Investigation of the physicochemical, bioactive properties and antioxidant potential of seeds of native fruits from Brazil: a study on the tucumã (Astrocaryum vulgare), bacupari (Garcinia gardneriana) and pupunha (Bactris gasipaes).","authors":"Larissa S Gualberto, Andréia Ibiapina, Bianca B Dias, Bárbara C B DE Freitas, Antônio A DE Melo Filho, Rômulo A Morais, Glêndara A S Martins","doi":"10.1590/0001-3765202520240862","DOIUrl":"10.1590/0001-3765202520240862","url":null,"abstract":"<p><p>The seeds of bacupari, tucumã, and peach palm fruits have distinct characteristics that significantly influence their industrial applications and nutritional value. These seeds have slightly acidic pH levels (5.88 - 6.79), indicating the presence of organic acids. The tucumã seed, for instance, stands out with a high lipid content of 19.35 g 100 g-1, contributing to a high energy value, while the bacupari seed has low lipid levels (0.73 g 100 g-1). Bacupari seeds exhibit high antioxidant potential using the β-carotene/linoleic acid method (244% inhibition) and vitamin C (91.17 mg AA 100 g-1). There were significant variations in phenolic compounds and antioxidant capacity among the seeds analyzed. The fatty acid profile, with a predominance of saturated acids, 90.42 and 88.66%, for tucumã and peach palm oil, respectively. However, the high atherogenicity and thrombogenicity rates suggest caution in consumption. The analysis of triacylglycerol molecules in pupunha and tucumã seeds demonstrated the predominance of mono-, di- and triacylglycerols. When analyzing the acidity index throughout the storage period, a clear trend towards an increase in its results is observed. Therefore, proposing control measures is essential for its correct use.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240862"},"PeriodicalIF":1.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Larissa Millena Girotto, Suelen P R Herrig, Maria Graciela I F Nunes, Otavio A Sakai, Beatriz C B Barros
{"title":"Extraction of phenolic compounds from Pfaffia glomerata leaves and evaluation of composition, antioxidant and antibacterial properties.","authors":"Larissa Millena Girotto, Suelen P R Herrig, Maria Graciela I F Nunes, Otavio A Sakai, Beatriz C B Barros","doi":"10.1590/0001-3765202520240317","DOIUrl":"10.1590/0001-3765202520240317","url":null,"abstract":"<p><p>This work aimed to evaluate the extraction conditions of total phenolic compounds (TPC) from Pfaffia glomerata leaves (PGLs) and characterize the extract obtained in the best conditions. Aqueous extraction was performed in a Shaker, varying the levels of rotation (100, 150, 200 rpm), temperature (30, 45, 60° C) and mass to volume (w v-1) ratio (1:10, 1:20, 1:30 g ml-1). The variables w v-1 ratio and temperature, and their combination, showed a positive effect (p<0.05) in TPC extraction. The time of extraction increased TPC extraction until 30 min, thereafter, the values decreased. The extraction performed at 60o C, 1:30 g ml-1, 125 rpm and 30 min allowed to reach the maximum TPC content (11.94 mg g-1). This extract contains β-ecdysone (4.64 g 100g-1), a chemical marker of P. glomerata, and, phenolic compounds, as gallic acid (28.51 mg 100g-1) and catechin (24.82 mg 100g-1). PGLs extract exhibits antioxidant activity by the in vitro methods evaluated (iron reduction powder and radical scavenging ability). Antibacterial activity was also detected, being found the minimum inhibitory concentration of 20 mg mL-1 for Escherichia coli and Pseudomonas aeruginosa. Therefore, PGL extract had a potential application as natural antioxidant and antimicrobial in food or pharmaceutical products.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20240317"},"PeriodicalIF":1.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isabela L Iessi, Bruna Dallaqua, Yuri K Sinzato, Franciane Q Gallego, Jens H Nielsen, Gustavo T Volpato, José E Corrente, Débora C Damasceno
{"title":"Maternal diabetes on fetal endocrine pancreas development in rats.","authors":"Isabela L Iessi, Bruna Dallaqua, Yuri K Sinzato, Franciane Q Gallego, Jens H Nielsen, Gustavo T Volpato, José E Corrente, Débora C Damasceno","doi":"10.1590/0001-3765202520240807","DOIUrl":"10.1590/0001-3765202520240807","url":null,"abstract":"<p><p>To evaluate if an unfavorable maternal environment induced by hyperglycemia, hypoxia, and oxidative stress status impairs the morphological development of pancreatic islets in the fetuses on days 18 and 21 of pregnancy. Wistar rats were injected with streptozotocin for diabetes induction. At adulthood (3 months), all animals underwent an oral glucose tolerance test (OGTT) for glucose assessment as an inclusion criterion. Following, the animals were mated. On day 18 and 21 of pregnancy, the mothers were killed for blood biochemical data and, fetal pancreas was collected for immunohistochemical analysis. On the GD18/21, the diabetic (D) dams showed higher glycemia, erythropoietin and TBARS levels, and a disorganized cell distribution in fetal pancreatic islets compared to control (C) rat mothers. The fetal endocrine pancreas of D dams presented a higher ratio of insulin-stained cells on GD18. No difference in the immunostaining for PDX-1 was observed. Fetuses of D dams showed a lower ratio of cells immunostained for Ki-67 in GD18 and greater cell proliferation in GD21. At the GD21, the D group showed a higher ratio of cells undergoing apoptosis. Maternal hyperglycemia impairs fetal pancreatic islet structure, which causes functional changes contributing to fetal hyperglycemia at birth.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240807"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edson L Ponte, Alana F Pires, Diego F Araujo, Timna V Martins, Renata Gorjão, Gilson M Murata, Pedro Marcos G Soares, Rui Curi, Ana Maria S Assreuy
{"title":"Inhibitory effect of trans-anethole in acute inflammation: involvement of macrophage-derived mediators.","authors":"Edson L Ponte, Alana F Pires, Diego F Araujo, Timna V Martins, Renata Gorjão, Gilson M Murata, Pedro Marcos G Soares, Rui Curi, Ana Maria S Assreuy","doi":"10.1590/0001-3765202520231288","DOIUrl":"10.1590/0001-3765202520231288","url":null,"abstract":"<p><p>This study investigated in vivo and in vitro the trans-anethole anti-inflammatory activity on vascular and cell events and the involvement of inflammatory mediators. In vivo Wistar male rats (180 - 200 g; n=6/group) received trans-anethole per oral and the anti-inflammatory activity was assessed on the models of paw edema, peritonitis and air pouch. In vitro, peritoneal macrophages were incubated with trans-anethole to evaluate cytotoxicity, cytokines and reactive oxygen species (ROS) stimulated by phorbol myristate acetate (PMA). In vivo trans-anethole (1 mg/kg) inhibited: edema (42%), vascular permeability (58%), migration of total leukocytes (50%) and neutrophils (42%), IL-1β (75%), IL-6 (61%), macrophage inflammatory protein-MIP-3α (64%), NO2 -/NO3 - (35%), malondialdehyde (MDA) and histological alterations induced by carrageenan. Trans-anethole (25 μM) also inhibited polymorphonuclear migration (90%), MIP-3α (83%) and TNF-α (60%) challenged by LPS. In vitro trans-anethole (10 - 50 μM) increased IL-6 per se and reduced inflammatory mediators released by macrophages: TNF-α (up to 80%) and IL-1β (up to 81%) challenged by LPS, and ROS (up to 43%) by PMA, without causing cytotoxicity. In conclusion, trans-anethole has anti-inflammatory effects on vascular and cellular events of acute inflammation via inhibition of cytokines and free radicals produced by macrophages.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20231288"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lorena O Fernandes-Siqueira, Raphael R A Melo, Luciana S Wermelinger, Fabio C L Almeida, Didier Salmon, Gustavo C Ferreira, Andrea T DA Poian
{"title":"An institutional COVID-19 initiative: creation of a biobank and serological data analysis in pre- and post-vaccination cohorts.","authors":"Lorena O Fernandes-Siqueira, Raphael R A Melo, Luciana S Wermelinger, Fabio C L Almeida, Didier Salmon, Gustavo C Ferreira, Andrea T DA Poian","doi":"10.1590/0001-3765202520240819","DOIUrl":"10.1590/0001-3765202520240819","url":null,"abstract":"<p><p>The COVID-19 pandemic has left a legacy in the management of health emergencies, but sentinel surveillance was relatively underused, despite its significant role in decision-making during epidemics. Here we describe a sentinel surveillance for anti-SARS-CoV-2 serology carried out on a cohort of 395 individuals at a Brazilian institution, from October 2020 to December 2022. A total of 1,507 serum samples were analyzed for IgG and IgA against SARS-CoV-2 Spike (S) or nucleocapsid (N) proteins, in the pre- and post-vaccination periods. The latter included two doses of CoronaVac (group 1, G1), or ChAdOx-1 or BNT162b2 (group 2, G2), followed by heterologous booster doses. In the pre-vaccination phase, 26.5% of the participants showed IgG reactivity for S and 13.7% for N. After the vaccines' first dose, S IgG response was positive in 66.6% or 98% of G1 or G2 participants, respectively, whereas 100% of the participants showed S IgG positivity after the second dose, and S IgG and IgA after the booster. This initiative enabled the examination of viral transmission beyond hospital environments, which has rarely been explored, and established protocols for managing future emergencies. In addition, a serum bank and a comprehensive database are now available to the scientific community.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240819"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cosme Teixeira DA Silva, Tadayuki Yanagi Junior, Raphael Winckler DE Bettio, Marcelo Bahuti
{"title":"Design of wireless web-based multiplatform system for thermal environmental control of broiler facilities using fuzzy set theory.","authors":"Cosme Teixeira DA Silva, Tadayuki Yanagi Junior, Raphael Winckler DE Bettio, Marcelo Bahuti","doi":"10.1590/0001-3765202520240032","DOIUrl":"10.1590/0001-3765202520240032","url":null,"abstract":"<p><p>The control and monitoring process for broiler facilities needs to be improved to mitigate or eliminate birds' thermal stress. Thus, the objective was to develop of a fuzzy controller embedded in a microcontroller and a multiplatform web application that communicates with the fuzzy controller to control the aviary climate system. An architecture based on the Internet of Things (IoT) and agribusiness 4.0 was used to implement a fuzzy controller embedded in a microcontroller. Data were collected by temperature and humidity sensors. A multiplatform web application communicated the climate system to the fuzzy controller. This information was transmitted to the central web server via message queuing telemetry transport (MQTT). The system performed decision-making to control the aviary thermal environment of broilers ranging from one to 49 days old. The input variables of the system were the black globe-humidity index (BGHI) and the bird age. Defuzzification by the center of gravity method produced environmental ratings that were used to control the thermal environment automatically and smartly. Using the intelligent prototype produced a 98% accuracy in the validation process. This low-cost system can be used as an agribusiness 4.0 application to mitigate thermal stress conditions in aviaries and, consequently, reduce productivity losses.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 1","pages":"e20240032"},"PeriodicalIF":1.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}