Ellen Aparecida G Bezerra, Renata B A Soares, Mauro Aparecido S Xavier, Maximino A Bezerra Júnior, Wilson Vicente S Pereira, Cassia S M Godoy, Marilia R Guimarães, Lúcia C Andrade, Alessandra Rejane E O Xavier
{"title":"Molecular identification of Histoplasma capsulatum in patients with disseminated histoplasmosis and acquired immunodeficiency syndrome.","authors":"Ellen Aparecida G Bezerra, Renata B A Soares, Mauro Aparecido S Xavier, Maximino A Bezerra Júnior, Wilson Vicente S Pereira, Cassia S M Godoy, Marilia R Guimarães, Lúcia C Andrade, Alessandra Rejane E O Xavier","doi":"10.1590/0001-3765202420231287","DOIUrl":"https://doi.org/10.1590/0001-3765202420231287","url":null,"abstract":"<p><p>Histoplasmosis is caused by the fungus Histoplasma capsulatum and is often fatal for individuals with acquired immunodeficiency syndrome (AIDS). Delayed diagnosis is a major factor in worsening coinfection, as it can be mistaken for other diseases. Thus, rapid identification of Histoplasma in immunocompromised patients is essential. Molecular techniques, particularly polymerase chain reaction (PCR), were used in this study to identify H. capsulatum in patients coinfected with histoplasmosis and AIDS. Blood samples from 14 individuals with AIDS and disseminated histoplasmosis were collected and analyzed. The PCR method successfully amplified the fungal region in whole blood samples, while PCR-RFLP analysis confirmed a consistent profile in the samples. Genetic sequencing further confirmed the fungal species. Compared to clinical tests such as fungal culture and urinary antigen detection, molecular analysis proved faster, more sensitive, and cost-effective. These molecular markers can potentially be incorporated into routine diagnostics in the future. Further studies are needed to expand and enhance this diagnostic approach, particularly in patients with nonprogressive clinical forms of histoplasmosis.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 1","pages":"e20231287"},"PeriodicalIF":1.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Potential developmental effects of licit and illicit substances in humans: An approach to risk-specific dose and incidence.","authors":"Arnaldo R Santos, Carolline M Lima","doi":"10.1590/0001-3765202420240445","DOIUrl":"10.1590/0001-3765202420240445","url":null,"abstract":"<p><p>Teratogens encompass any agent capable of causing a birth defect or elevating the incidence of defects within the population. This category includes substances like drugs, both legal and illegal. These substances cause congenital anomalies depending on the stage of development at the time of exposure, the dose, and the exposure time associated with the embryo. The most sensitive period is the embryonic stage, when the three leaflets give rise to tissues and organs. Susceptibility to teratogenesis decreases during the fetal phase but morphological and functional disturbance of the fetus may still occur. Substance use during pregnancy and its adverse effects are a public health problem and the lay population does not have access to this information. Particularly concerning is the period within the first six weeks of pregnancy, often before a woman realizes she is pregnant. Developmental data for many substances are simply not available, which makes the problem more serious. The aim of this study is to reflect on the teratogenic effects of licit and illicit substances in humans, focusing particularly on the dose that can induce malformations and their incidence in humans.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 1","pages":"e20240445"},"PeriodicalIF":1.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Irlane Cristine S A Lira, Natoniel F DE Melo, Rafaela Priscila Antonio, Ronaldo S DE Oliveira, Manoel A DE Queiroz
{"title":"The chromosome number and heterochromatin distribution pattern suggest differentiation among species of the Stylosanthes scabra-seabrana complex.","authors":"Irlane Cristine S A Lira, Natoniel F DE Melo, Rafaela Priscila Antonio, Ronaldo S DE Oliveira, Manoel A DE Queiroz","doi":"10.1590/0001-3765202420240247","DOIUrl":"https://doi.org/10.1590/0001-3765202420240247","url":null,"abstract":"<p><p>The genus Stylosanthes has economic importance in semi-arid regions and requires studies that reveal complex relationships between species involving different ploidies. The aim of this study was to cytogenetically investigate accessions of Stylosanthes identified as S. scabra, in order to properly identify the number, morphology, and pattern of distribution of heterochromatin, analyzing the karyological variability of these species. Accessions with 2n=40 and 2n=20 were identified, exhibiting semi-reticulate interphase nuclei, symmetric karyotype, varied morphology, with differences in average chromosomal size, and genome length. The analysis with the fluorochromes chromomycin (CMA) and 4',6-diamidino-2-phenylindole (DAPI) allowed the visualization of two CMA+ blocks in the subterminal region of the short arm in all accessions, and DAPI+/CMA- bands in S. scabra accessions. This suggests that not only chromosomal rearrangements, but also changes in the composition of heterochromatin, may have occurred during the speciation of this genus, and that S. scabra may be undergoing chromosomal evolution based on the observed karyological differences. In addition to the ploidy level, the distribution pattern of CMA+ heterochromatin reinforces the separation between S. scabra and S. seabrana. Thus, this genus represents an interesting group of plants for further studies on the content and quantity of repetitive and non-repetitive DNA sequences.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 1","pages":"e20240247"},"PeriodicalIF":1.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tainara R Menna-Barreto, Ana Emília Siegloch, Sandro Santos, Tiago G Dos Santos, Marcos André P Velloso, Marcia Regina Spies
{"title":"Aquatic insects in subtropical streams: the role of different grassland ecosystems and local environmental descriptors.","authors":"Tainara R Menna-Barreto, Ana Emília Siegloch, Sandro Santos, Tiago G Dos Santos, Marcos André P Velloso, Marcia Regina Spies","doi":"10.1590/0001-3765202420230918","DOIUrl":"https://doi.org/10.1590/0001-3765202420230918","url":null,"abstract":"<p><p>Organisms can respond to environmental gradients from local to landscape features. Aquatic insects are particularly affected by watershed peculiarities due to their dependence on microhabitat conditions. However, these relationships are poorly understood in lotic ecosystems of subtropical grasslands, limiting water resources management and bioassessment proposals. Here, we investigated how local stream environment and variations in landscape types affect the assemblage structure of a bioindicator insect group, face to the spatial proximity of the sampled locations. We sampled immatures of Ephemeroptera, Plecoptera, and Trichoptera in streams along the Brazilian Pampa biome, recording environmental descriptors in different grassland ecosystem types. The structure of aquatic insect assemblages differed across grassland types, with specific dominant genera associated with each landscape. Spatially-structured water physicochemical descriptors explained a significant amount of variation in assemblage data. Our findings suggest that grassland ecosystem type delimitations capture ecological attributes, influencing watershed features important to EPT assemblage structuration. Moreover, we highlight the importance of niche-based process structuring EPT assemblages along grassland ecosystem types of Pampa biome. In addition, we encourage using aquatic insects in bioassessment of lotic waters to assess local and landscape environmental impacts. We strongly recommend considering the grassland ecosystem schedule for water resources management and bioassessment proposals.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20230918"},"PeriodicalIF":1.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beatriz Helena Brugnaro, Camila R G Lima, Isabelle G R DA Costa, Gesica Fernandes, Ana Carolina DE Campos, Peter Rosenbaum, Silvia Letícia Pavão, Nelci Adriana C F Rocha
{"title":"Personal and environmental factors and household assistance in Down syndrome: cross-sectional study.","authors":"Beatriz Helena Brugnaro, Camila R G Lima, Isabelle G R DA Costa, Gesica Fernandes, Ana Carolina DE Campos, Peter Rosenbaum, Silvia Letícia Pavão, Nelci Adriana C F Rocha","doi":"10.1590/0001-3765202420230558","DOIUrl":"https://doi.org/10.1590/0001-3765202420230558","url":null,"abstract":"<p><p>According to a biopsychosocial framework, personal and environmental factors might be mediators or facilitators/barriers, respectively, to functioning. However, it is not known how these factors can impact independence in household chores in children/adolescents with Down syndrome (DS). This study explored whether and how personal/environmental factors are associated with the independence level in household chores of children/adolescents with DS in Brazil. Caregivers of twenty-eight children/adolescents with DS were interviewed using the CHORES and a standardized questionnaire about personal (child's age and sex) and environmental (socioeconomic level and maternal schooling) factors. Multiple linear regression analysis identified if/how these factors are associated with level of independence. For CHORES self-care and CHORES total, sex was a significant variable explaining 21.8% and 15.8%, respectively, of the variation in the outcomes. For the outcome CHORES family care none of the variables was significant. Female sex was associated with a lower need for assistance. We conclude that only the personal factor assessed related to female sex in children with DS was associated with the independence level in household chores. This finding highlights the importance of health care providers and families to encourage the independence in chores regardless of sex and promote opportunities for both boys and girls.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 1","pages":"e20230558"},"PeriodicalIF":1.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Breno G C Alvim, Renato Jose P Machado, Tiago K Krolow
{"title":"Review of the Brazilian species of the genus Climaciella Enderlein (Neuroptera: Mantispidae), with the description of two new species.","authors":"Breno G C Alvim, Renato Jose P Machado, Tiago K Krolow","doi":"10.1590/0001-3765202420230788","DOIUrl":"https://doi.org/10.1590/0001-3765202420230788","url":null,"abstract":"<p><p>Climaciella Enderlein is a genus of the family Mantispidae (Neuroptera) restricted to the New World with records ranging from southern Canada to northern Argentina. The genus is currently represented by 12 valid species, three of them with records from Brazil: C. amapaensis Penny, C. duckei Navás, and C. semihyalina (Le Peletier & Audinet-Serville). Besides the relatively low number of species from Brazil, it is known that Climaciella species present some historical taxonomic problems. In this sense, the main goal of this study was to taxonomically revise the Climaciella species from Brazil, providing detailed (re)descriptions and illustrations of all species, an updated distribution map, and an identification key to the whole genus. The study resulted in some new taxonomic acts: C. tincta (Navás) is revalidated and C. duckei is considered a new synonym of C. tincta; two new species are described: Climaciella hoffmani sp. nov. and Climaciella pennyi sp. nov.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20230788"},"PeriodicalIF":1.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Camila B Souza, Rene Francisco B Gonçalves, José Atílio F F Rocco
{"title":"Analysis of Tribology Properties of Trimethylolpropane-based Lubricant by Molecular Dynamics.","authors":"Camila B Souza, Rene Francisco B Gonçalves, José Atílio F F Rocco","doi":"10.1590/0001-3765202420240040","DOIUrl":"https://doi.org/10.1590/0001-3765202420240040","url":null,"abstract":"<p><p>Currently, it is crucial for the lubricant formulation industry to explore cost-effective and environmentally friendly methodologies for analyzing the tribological properties of engine aviation lubricants under high-temperature and high-pressure operating conditions. This study demonstrates the feasibility of employing molecular dynamic simulations to gain essential insights into the evolution of the tribological properties of lubricants during operation. A three-layer molecular model was devised, comprising nickel aluminide molecules in the top and bottom layers, and polyol ester in the core. The impact of sliding velocities ranging from 20 km/h to 100 km/h was investigated under varying temperature and pressure conditions. Concentration, temperature and velocity profiles, radial distribution function, mean square displacement, and friction coefficient were calculated and analyzed in detail. Notably, the highest friction coefficients - ranging from 2.5 to 0.75 - were observed at the lowest temperature and pressure conditions tested. Conversely, other sections of the gas turbine exhibited substantially lower friction coefficients - ranging from 0 to 0.01.Simulations demonstrate that increasing pressure and temperature reduce polymer chain mobility, leading to stronger internal interactions within the lubricant. Consequently, lubricant adsorption onto metal surfaces decreases. Furthermore, the lubricant performs exceptionally well when its molecules encounter higher velocities and temperatures. Based on the results obtained, the research demonstrates that the presented technique provides both quantitative and qualitative tribological information essential for understanding a system molecular behavior, serving as a guiding framework for researchers in the field.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 1","pages":"e20240040"},"PeriodicalIF":1.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lucas F Quartarolli, Fernando M DE Melo, Joaniel M Martins, Alceu T Silveira-Junior, Marcelo Nakamura, Henrique E Toma
{"title":"New insights on the Jacobsite mineral from Bahia and related synthetic manganese-ferrite nanoparticles.","authors":"Lucas F Quartarolli, Fernando M DE Melo, Joaniel M Martins, Alceu T Silveira-Junior, Marcelo Nakamura, Henrique E Toma","doi":"10.1590/0001-3765202420240234","DOIUrl":"https://doi.org/10.1590/0001-3765202420240234","url":null,"abstract":"<p><p>Jacobsite is a relatively rare mineral of composition MnFe2O4, found in Urandi (Bahia State) in Brazil. It is also a common species in the deep-sea manganese nodules, attracting the interest of many mineral-extracting companies. Because of its spinel constitution similar to magnetite, Jacobsite is commonly called a manganese-ferrite. However, the manganese/iron content may vary substantially according to its origin, demanding specific studies in each case. The Jacobsite mineral inspired our laboratory synthesis of the analogous manganese ferrite nanoparticles. The direct synthesis by the coprecipitation method has not been successful; however, it can be carried in the presence of citrate ions, yielding strongly magnetic nanoparticles, with a maximum magnetization of 45.6 emu.g1. Although they were structurally identical to Jacobsite, the mineral from Bahia exhibited a rather weak magnetism, because it involves a ferrimagnetic coupling. For this reason, the synthetic method seems to provide a better way of obtaining strongly magnetic manganese ferrites. These magnetic nanoparticles have been investigated in detail, including their interaction with diatoms, providing interesting magnetic bio-silicate carriers in drug delivery.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 suppl 1","pages":"e20240234"},"PeriodicalIF":1.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Emergence of Edgar Morin's Complex Thinking.","authors":"Enio A Blay, José Roberto C Piqueira","doi":"10.1590/0001-3765202420231112","DOIUrl":"https://doi.org/10.1590/0001-3765202420231112","url":null,"abstract":"<p><p>Edgar Morin is more than 100 years old and has produced numerous original ideas. Complex Thinking is his approach to complexity and took almost thirty years to be written. He developed it based on many other thinkers but chiefly, we argue, on Wiener's Cybernetics, von Bertalanffy's General System Theory and Shannon's Information Theory. This article describes and discusses how those latter theories have been incorporated into Morin's thought, especially in La Méthode, his magnum opus, and presents, in a comparative fashion, his pros and contras on each of them. In our conclusion, we discuss how some of Morin's criticisms of the founding theories might be unjust and also present a summary of some judgmental appraisals of Complex Thinking.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 4","pages":"e20231112"},"PeriodicalIF":1.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A brief assessment of the influence of Animal Science in the Impact Factor of the AABC.","authors":"Alexander W A Kellner","doi":"10.1590/0001-376520242024963","DOIUrl":"https://doi.org/10.1590/0001-376520242024963","url":null,"abstract":"","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"96 3","pages":"e2024963"},"PeriodicalIF":1.1,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142279321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}