{"title":"First assessment of the metazoan parasite community of the Argentine menhaden Brevoortia pectinata (Jenyns, 1842) (Clupeiformes: Alosidae) off Rio de Janeiro, southeastern Brazil.","authors":"Luana Benicio, Fabiano Paschoal, Felipe B Pereira","doi":"10.1590/0001-3765202520240683","DOIUrl":"https://doi.org/10.1590/0001-3765202520240683","url":null,"abstract":"<p><p>Brevoortia pectinata is important for the fish industry and crucial for the ecology of South Atlantic marine environments. Despite this importance, the structure of the parasite community of B. pectinata has never been investigated. We evaluated for the first time the parasite community structure from a population of B. pectinata, off the State of Rio de Janeiro, Brazil, and the factors influencing it. One hundred fish were bought from local fisherman at Sepetiba Bay (22°57'44\"S; 43°52'28\"W). Detailed parasitological examination revealed that all fish were parasitized by at least one parasite. We found 14 parasite taxa: 3 monogeneans, 4 copepods, 1 isopod, 3 digenetics and 3 nematode larvae. Presence of both adult and larvae indicated that B. pectinata occupies an intermediate trophic level, acting as definitive and intermediate / paratenic host. The community had low diversity and was dominated by the monogeneans Mazocraeoides georgei and Kuhnia sp., whereas the endoparasites were less prevalent and abundant. Fish schools and host specificity seem to be important for ectoparasite infestation, and host diet influenced the infection by trophic-transmitted endoparasites. Fish sex and body length exerted weak influence in the parasite community structure, which was depauperate and non-interactive.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20240683"},"PeriodicalIF":1.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vijai M Moorthy, Bhupal N Dharamsoth, Vijayalakshmi Muthukaruppan, Arul Elango, Kalaiarasi Ganesan
{"title":"Predicting Coronary Heart Disease Using Data Mining and Machine Learning Solutions.","authors":"Vijai M Moorthy, Bhupal N Dharamsoth, Vijayalakshmi Muthukaruppan, Arul Elango, Kalaiarasi Ganesan","doi":"10.1590/0001-3765202520240811","DOIUrl":"https://doi.org/10.1590/0001-3765202520240811","url":null,"abstract":"<p><p>This research focuses on predicting cardiovascular disease using machine learning classification strategies. The study presents a unique approach by integrating multiple machine learning techniques, leveraging the strengths of Random Forest and Gradient Boosting. The authors developed a novel ensemble learning model, combining Linear Regression, Random Forest, and Gradient Boosting algorithms, optimized using Bayesian hyperparameter tuning. The model demonstrated superior performance in predicting CVD outcomes, with classification accuracy of 95.5%, 94.26%, and 98.3% for Linear Regression, Decision Tree, and Gradient Boosted methods, respectively. The true positive rate for the GB algorithm's predictions of patients was 98.3%. The study hypothesizes that the GB method predicts the Framingham dataset better than other algorithms using 4240 samples.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20240811"},"PeriodicalIF":1.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suellen Maria A DA Silva, João Kaycke S DA Silva, Joice Fragoso O DE Araújo, Andressa Letícia L DA Silva, Andresa Katherine A DE Almeida, Tamires A DO Nascimento, André Felippe A Xavier, Renata G DE Almeida, Guilherme Augusti M Jardim, Eufrânio N DA Silva Júnior, Vivaldo Moura-Neto, Alysson Wagner F Duarte, Pierre Barnabé Escodro, João Xavier DE Araújo Júnior, Marília O F Goulart, Aline C DE Queiroz, Magna Suzana Alexandre-Moreira
{"title":"In vitro Antitumor Activity and Electrochemical Studies of Bio-Electroactive Anthraquinone Derivatives in Glioblastoma.","authors":"Suellen Maria A DA Silva, João Kaycke S DA Silva, Joice Fragoso O DE Araújo, Andressa Letícia L DA Silva, Andresa Katherine A DE Almeida, Tamires A DO Nascimento, André Felippe A Xavier, Renata G DE Almeida, Guilherme Augusti M Jardim, Eufrânio N DA Silva Júnior, Vivaldo Moura-Neto, Alysson Wagner F Duarte, Pierre Barnabé Escodro, João Xavier DE Araújo Júnior, Marília O F Goulart, Aline C DE Queiroz, Magna Suzana Alexandre-Moreira","doi":"10.1590/0001-3765202520241466","DOIUrl":"https://doi.org/10.1590/0001-3765202520241466","url":null,"abstract":"<p><p>This study investigated the antiglioblastoma potential of nine synthetic anthraquinone derivatives (SAQDs). SAQDs were tested for their in vitro cytotoxicity against PBMCs and GBM02 cells. For PBMC, SAQDs showed no cytotoxic effects up to 100 µM. Four most promising SAQDs (1, 4, 5, and 9) demonstrated significant activity against GBM02, with selectivity index >4.54 for 1, >1.30 for 4 and 5, and >1.19 for 9. These compounds induced morphological changes, incluing cell rounding, cytoplasmic vacuolation, and membrane rupture, and inhibited cell migration. Due to the pharmacological role of oxidative stress, electrochemical methods were used to compare the reductive and oxidative capacities of the SAQD with their antitumor activity. Considering the reported findings, it is feasible to confirm that some SAQDs have antitumor effects, supporting additional studies of AQ for developing novel anticancer medications. Due to the small number of compounds investigated, it was not possible to observe a relationship between electrochemical and biological data. However, the electrochemical data are new and could be used to prepare other compounds on this line. CV data revealed that the sulfone's electron-withdrawing effect facilitated the reduction. At the same time, the sulfides had shown less positive potential for oxidation waves due to the presence of electron-donating groups.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20241466"},"PeriodicalIF":1.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Raquel O Simões, Thiago S Cardoso, Aldenice Nazaré Pereira, Fabiano Paschoal, José Luis Luque
{"title":"Parasite metacommunity structure of Mullus argentinae Hubbs & Marini, 1933 (Actinopterygii: Mullidae) off Rio de Janeiro, Southeastern Brazil.","authors":"Raquel O Simões, Thiago S Cardoso, Aldenice Nazaré Pereira, Fabiano Paschoal, José Luis Luque","doi":"10.1590/0001-3765202520240830","DOIUrl":"https://doi.org/10.1590/0001-3765202520240830","url":null,"abstract":"<p><p><italic>Mullus argentinae</italic> Hubbs & Marini, 1933 is a common demersal fish that inhabits the Brazilian and Argentinean coastal zone. A total of 200 specimens of <italic>M. argentinae </italic>were collected over the four seasons (spring, summer, autumn, and winter), from 2009 to 2011, from the littoral of the State of Rio de Janeiro, Brazil, in order to study their parasite community, including their composition and structure. The distribution pattern of the parasite species was investigated using metacommunity analysis, in addition to beta diversity calculations and their components. We investigated the influence of the seasonality (temporal factor) and host-related biological variables (body length and mass) on the occurrence and abundance of parasites throughout their hosts. A total of 12 parasite species were collected (nine endoparasites and three ectoparasites). The digenean <italic>Prosorhynchus </italic>sp. presented the highest values of abundance, intensity, and prevalence of infection. An influence of seasonality on the abundance of almost all analyzed parasite species, and on their distribution along the studied metacommunity was observed, which indicates that temporal factors may be important for the patterns of interaction of these parasites with their hosts.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20240830"},"PeriodicalIF":1.1,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Clara N Alves, Larissa D Fonseca, Rayana M Paixão, Adriano T Oliveira
{"title":"Occurrence of the hemoparasite Cyrilia spp. in the Amazonian stingray Potamotrygon wallacei.","authors":"Ana Clara N Alves, Larissa D Fonseca, Rayana M Paixão, Adriano T Oliveira","doi":"10.1590/0001-3765202520241477","DOIUrl":"https://doi.org/10.1590/0001-3765202520241477","url":null,"abstract":"<p><p>Freshwater stingrays play a crucial ecological role in the Amazon; however, studies on their parasitic fauna still need to be made available. This study aimed to investigate the occurrence of hemoparasites, specifically Cyrilia spp., in specimens of Potamotrygon wallacei from the Lower Rio Negro, Brazil. The research was conducted through nighttime fishing at Praia do Açutuba and the Tupé Sustainable Development Reserve. Blood samples were collected by puncturing branchial vessels, and smears were stained and analyzed under a microscope for parasitemia quantification. The results showed that specimens from Açutuba did not present significant parasites, except for one individual, which exhibited a possible macrogamont. In contrast, all specimens from Tupé displayed various parasitic stages, with parasitemia levels ranging from 0.2% to 1.3%. Morphological analysis identified predominantly intracellular stages such as trophozoites, pre-gamonts, macrogamonts, and microgamonts. It was noted that the observed stages were more similar to those characteristic of the genus Cyrilia spp. than to any other haemogregarines in fish. The study concluded that environmental and site-specific factors, such as habitat type, may influence parasitic distribution, with igapó areas favoring higher parasitic activity than beach areas.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20241477"},"PeriodicalIF":1.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gabriel R Antunes, Ana Júlia F Souza, Giovanni Y DA Silva, Julliane Yoneda, Elivelton A Ferreira, Diego P Sangi
{"title":"Nitromethylene N-heterocycles as water soluble corrosion inhibitors.","authors":"Gabriel R Antunes, Ana Júlia F Souza, Giovanni Y DA Silva, Julliane Yoneda, Elivelton A Ferreira, Diego P Sangi","doi":"10.1590/0001-3765202520240840","DOIUrl":"https://doi.org/10.1590/0001-3765202520240840","url":null,"abstract":"<p><p>Using a green methodology, we performed a double vinylic substitution on 1,1-bis(methylsulfanyl)-2-nitroethylene, synthesizing six 2-nitromethylene heterocyclic compounds. These compounds were evaluated as corrosion inhibitors, and in silico predictions were made to assess their toxicity risks. The synthesized compounds are water soluble and exhibit strong corrosion inhibition activity, effectively protecting metal against mass loss. Furthermore, the in silico toxicity predictions indicate that these compounds do not present significant toxicity risks, positioning them as innovative, environmentally friendly alternatives for organic corrosion inhibitors.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 3","pages":"e20240840"},"PeriodicalIF":1.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marco Antonio S Silva, Guilherme Dognani, André Luiz L DE Faria, Eduardo Nery D DE Araujo, Mitchael Micael S Gomes, Carlos José L Constantino, José Tadeu G Tommaselli, João Osvaldo R Nunes
{"title":"A simple method for mapping microplastics filter collectors for microscopic analyses.","authors":"Marco Antonio S Silva, Guilherme Dognani, André Luiz L DE Faria, Eduardo Nery D DE Araujo, Mitchael Micael S Gomes, Carlos José L Constantino, José Tadeu G Tommaselli, João Osvaldo R Nunes","doi":"10.1590/0001-3765202520250309","DOIUrl":"https://doi.org/10.1590/0001-3765202520250309","url":null,"abstract":"<p><p>Microplastic has stood out as one of the main contaminants derived from plastic wastes due to its slow decomposition rate and dangers to the aquatic environment, the ecosystem, and even human health. However, microplastic fragment detection, identification, removal, and quantification remain a challenge. One of the main methods of removing these fragments is the filtration process using filter membranes such as paper, glass fibers, wool, etc. Among several techniques for localizing and identifying these fragments, optical microscopy is an easily accessible alternative, however, the spatialization to locate these fragments has become a challenge for equipment operators. This work proposes a simple spatialization system using coordinates that reduce operation time to make the localization of microplastic fragments onto collector filters easy. In addition to the use of an optical microscope, a micro-Raman was used to demonstrate the efficiency of this spatialization system even in another type of microscope, reducing the operating time from 2 hours and 45 minutes to approximately 2 minutes and 20 seconds, making the search for the fragment more effective and consequently, a faster analysis process.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 suppl 3","pages":"e20250309"},"PeriodicalIF":1.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bad Science - what is in the pipeline?","authors":"Alexander W A Kellner","doi":"10.1590/0001-376520259702","DOIUrl":"https://doi.org/10.1590/0001-376520259702","url":null,"abstract":"","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 2","pages":"e20259702"},"PeriodicalIF":1.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dárcia Gabriela B DA Costa, Daralyns B Macedo, Carlos Willian D Dantas, Regianne M Dos Santos Correa, Ádria D Dos Santos, Hendrya Julianny P Coelho, Carla Giovanni Á Moreira, Heden Luiz M Moreira, Rommel Thiago J Ramos, Marília Danyelle N Rodrigues
{"title":"Development of microsatellite markers for Leiarius marmoratus obtained by partial de novo genome assembly.","authors":"Dárcia Gabriela B DA Costa, Daralyns B Macedo, Carlos Willian D Dantas, Regianne M Dos Santos Correa, Ádria D Dos Santos, Hendrya Julianny P Coelho, Carla Giovanni Á Moreira, Heden Luiz M Moreira, Rommel Thiago J Ramos, Marília Danyelle N Rodrigues","doi":"10.1590/0001-3765202520241094","DOIUrl":"https://doi.org/10.1590/0001-3765202520241094","url":null,"abstract":"<p><p>This study addresses the identification of repetitive regions called microsatellites in the genome of the Amazonian species <italic>Leiarius marmoratus</italic>. An efficient methodology was used with sequencing and genome assembly to obtain and map specific markers. The programs MISA and EasySSR identified 221,273 and 145,601 markers, respectively. In addition, primers were developed, of which 590 were considered successful, according to in-silico testing with the Batchprime3 program. The results confirm the quality of the methodology, which can be applied in studies of genetic improvement, variability control, and genomic and comparative analyses.</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97Suppl 2 Suppl 2","pages":"e20241094"},"PeriodicalIF":1.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pedro Henrique Cardoso, Fátima Regina G Salimena, Andrea P Luizi-Ponzo, Nádia Somavilla, Pâmela T Patrício, Marcelo Trovó
{"title":"Stachytarpheta serrana, a new species of Verbenaceae endemic to the Diamantina Plateau in Espinhaço Range (Minas Gerais, Brazil), with palynological and anatomical notes.","authors":"Pedro Henrique Cardoso, Fátima Regina G Salimena, Andrea P Luizi-Ponzo, Nádia Somavilla, Pâmela T Patrício, Marcelo Trovó","doi":"10.1590/0001-3765202520241271","DOIUrl":"https://doi.org/10.1590/0001-3765202520241271","url":null,"abstract":"<p><p>Stachytarpheta serrana, a new Brazilian species from the Serro municipality in the campos rupestres of the Diamantina Plateau, Minas Gerais state, is described and illustrated. Like most species of the genus found in this region, S. serrana has blue corollas and is morphologically related to S. glabra. Its recognition as a distinct species is supported by morphological, palynological, and anatomical evidence. The main traits for its recognition are its erect or suberect leaves, amphistomatic, with a foveolate abaxial surface and hirsute veins forming a reticulate network, flowers with a calyx 1.3-1.6 cm long and a corolla tube 2.3-2.6 cm long, subprolate pollen grains with a granulate colpi membrane, and fruits with a short beak at the apex. With a restricted distribution and known from only two recent collections, S. serrana should be classified as Data Deficient (DD).</p>","PeriodicalId":7776,"journal":{"name":"Anais da Academia Brasileira de Ciencias","volume":"97 suppl 2","pages":"e20241271"},"PeriodicalIF":1.1,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144224054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}