Salmonella spp. detection in fresh and freeze-dried frog meat: a comparison of methods.

IF 1.1 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Anais da Academia Brasileira de Ciencias Pub Date : 2025-03-21 eCollection Date: 2025-01-01 DOI:10.1590/0001-3765202520230552
Silvia Conceição R P Mello, Rossiane M DE Souza, Eliane Rodrigues, Fernando David Caracuschansk, Beatriz V Mendes, Eliana DE Fátima M DE Mesquita
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引用次数: 0

Abstract

This study examined the presence of Salmonella spp. in fresh frog meat and freeze-dried seasoned frog meat stored for up to 90 days at room temperature. The samples were analyzed in triplicate using the conventional official method, by polymerase chain reaction (PCR) and mass spectrometry technique (MALDI -TOF) - for a comparison of the results obtained by the three tested methods. Mass spectrometry analyses confirmed the presence of Salmonella spp. in 100% of the fresh frog meat samples. In the analysis by the conventional method, the percentages of detection were 67% and 100% in samples B2 and B3, whereas the PCR method indicated 100% and 67% presence of the bacteria in the respective samples. In the analyses of freeze-dried none of the three methods detected the presence of Salmonella spp. The different analysis methods tested in this study were efficient in determining the presence or absence of Salmonella spp. in the samples, and the comparison between methods revealed a high percentage of compatibility of results. Adoption of efficient detection methods, such as PCR and MALDI-TOF, is essential, not only for public health, but also for compliance with Brazilian regulations, ensuring that food products are free from Salmonella spp. contamination and safe for consumption.

鲜、冻干蛙肉中沙门氏菌的检测方法比较。
本研究检测了在室温下保存90天的新鲜蛙肉和冻干调味蛙肉中沙门氏菌的存在。采用传统的官方方法,采用聚合酶链反应(PCR)和质谱技术(MALDI - tof)对三份样品进行分析,以比较三种测试方法获得的结果。质谱分析证实在100%的新鲜蛙肉样品中存在沙门氏菌。在常规方法的分析中,样品B2和B3的检出率分别为67%和100%,而PCR方法的检出率分别为100%和67%。在冻干样品的分析中,三种方法均未检出沙门氏菌。本研究中所测试的不同分析方法对样品中是否存在沙门氏菌都是有效的,并且两种方法之间的比较表明结果的相容性很高。采用有效的检测方法,如聚合酶链反应和MALDI-TOF,不仅对公共卫生至关重要,而且对遵守巴西法规至关重要,确保食品不受沙门氏菌污染,可安全食用。
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来源期刊
Anais da Academia Brasileira de Ciencias
Anais da Academia Brasileira de Ciencias 综合性期刊-综合性期刊
CiteScore
2.20
自引率
0.00%
发文量
347
审稿时长
1 months
期刊介绍: The Brazilian Academy of Sciences (BAS) publishes its journal, Annals of the Brazilian Academy of Sciences (AABC, in its Brazilianportuguese acronym ), every 3 months, being the oldest journal in Brazil with conkinuous distribukion, daking back to 1929. This scienkihic journal aims to publish the advances in scienkihic research from both Brazilian and foreigner scienkists, who work in the main research centers in the whole world, always looking for excellence. Essenkially a mulkidisciplinary journal, the AABC cover, with both reviews and original researches, the diverse areas represented in the Academy, such as Biology, Physics, Biomedical Sciences, Chemistry, Agrarian Sciences, Engineering, Mathemakics, Social, Health and Earth Sciences.
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