用部分从头基因组组装获得的黑鲈微卫星标记的开发。

IF 1.1 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Anais da Academia Brasileira de Ciencias Pub Date : 2025-05-30 eCollection Date: 2025-01-01 DOI:10.1590/0001-3765202520241094

Dárcia Gabriela B DA Costa, Daralyns B Macedo, Carlos Willian D Dantas, Regianne M Dos Santos Correa, Ádria D Dos Santos, Hendrya Julianny P Coelho, Carla Giovanni Á Moreira, Heden Luiz M Moreira, Rommel Thiago J Ramos, Marília Danyelle N Rodrigues

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引用次数: 0

摘要

本研究解决了亚马逊物种leariarius marmoratus基因组中称为微卫星的重复区域的鉴定。一种有效的方法被用于测序和基因组组装来获得和绘制特定的标记。MISA和EasySSR程序分别鉴定出221,273和145,601个标记。此外，还开发了引物，根据Batchprime3程序的计算机测试，其中590个引物被认为是成功的。结果证实了该方法的质量，可以应用于遗传改良、变异控制、基因组和比较分析的研究。

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Development of microsatellite markers for Leiarius marmoratus obtained by partial de novo genome assembly.

This study addresses the identification of repetitive regions called microsatellites in the genome of the Amazonian species Leiarius marmoratus. An efficient methodology was used with sequencing and genome assembly to obtain and map specific markers. The programs MISA and EasySSR identified 221,273 and 145,601 markers, respectively. In addition, primers were developed, of which 590 were considered successful, according to in-silico testing with the Batchprime3 program. The results confirm the quality of the methodology, which can be applied in studies of genetic improvement, variability control, and genomic and comparative analyses.

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来源期刊

Anais da Academia Brasileira de Ciencias 综合性期刊-综合性期刊

CiteScore

2.20

自引率

0.00%

发文量

347

审稿时长

1 months

期刊介绍： The Brazilian Academy of Sciences (BAS) publishes its journal, Annals of the Brazilian Academy of Sciences (AABC, in its Brazilianportuguese acronym ), every 3 months, being the oldest journal in Brazil with conkinuous distribukion, daking back to 1929. This scienkihic journal aims to publish the advances in scienkihic research from both Brazilian and foreigner scienkists, who work in the main research centers in the whole world, always looking for excellence. Essenkially a mulkidisciplinary journal, the AABC cover, with both reviews and original researches, the diverse areas represented in the Academy, such as Biology, Physics, Biomedical Sciences, Chemistry, Agrarian Sciences, Engineering, Mathemakics, Social, Health and Earth Sciences.