{"title":"[Latin American journals: a grain of sand in universal science].","authors":"A M Cetto","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"647-50"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20051352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro activity of fluconazole on Candida albicans.","authors":"L C Abecia, J M Arévalo, M J López","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fluconazole is a triazole antifungal compound suitable for the treatment of fungal infections, including those caused by Candida albicans. Fluconazole, as all azole antifungals, is a potent inhibitor of ergosterol biosynthesis. The aims of this study are to evaluate the susceptibility of C. albicans strains isolated from clinical specimens against fluconazole, and to assess the decrease in ergosterol produced on these strains when they are incubated in vitro with this antifungal compound. Sixty six yeast strains were isolated and identified from vaginal specimens of 710 women of a tocogynecology surgery (9.3%), C. albicans being the most frequent species (n = 52, ca. 79%). An agar dilution technique was used to determine the minimal inhibitory concentration (MIC) of fluconazole for the C. albicans strains. The MICs rank was between 1 and 20 micrograms/ml (mean = 6.6 micrograms/ml). Ergosterol content from ten C. albicans strains (MIC for fluconazole = 5 micrograms/ml) was assessed using the method proposed by Breivik and Owades, with three concentrations of fluconazole (2.5, 5 and 20 micrograms/ml) and four contact times (1, 6, 12 and 24 h), in comparison to no treated strains (control). The mean content of ergosterol was lower in the treated strains than in the control ones, and became statistically significant after 12 h of incubation.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"613-20"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[David Vázquez (1930-1985). Memories of an era].","authors":"J R Villanueva","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"631-40"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20051350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of nutrients on alginate synthesis in Azotobacter vinelandii and characterization of the produced alginate.","authors":"S A Sabry, K M Ghanem, W A Sabra","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The role of nutrients on alginate production by Azotobacter vinelandii was studied in batch cultures. The largest amount of bacterial alginate was obtained in presence of: 0.3 g/l MgSO4.7H2O. 0.4 g/l NaCl, 42 mg/l CaCl2.2H2O,.4 mg/l KH2PO4, 16 mg/l K2HPO4, 2.5 mg/l FeSO4.7H2O, 2.9 mg/l H3BO3, 2 mg/l ZnSO4.7H2O, 2 mg/l Na2MoO4.2H2O, 0.3 mg/l CuSO4.5H2O, 0.2 mg/l MnCl2.4H2O. Alginate production was not enhanced by natural additives or inducing agents, except for acetate, which increased alginate yield. The pure alginate contained 0.36% ash and 0.4% protein. It is similar to algal alginate, but it has an extra acetyl group. It contains 69.5% M-M block, 27.5% M-G block and 3% G-G block.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"593-8"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T Boquete, A Vindel, C Martin-Bourgon, L Azañedo, J A Sáez-Nieto
{"title":"Epidemiological markers of Serratia marcescens isolates causing nosocomial infections in Spain (1981-1991).","authors":"T Boquete, A Vindel, C Martin-Bourgon, L Azañedo, J A Sáez-Nieto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The distribution of epidemiological markers (serotyping and phage-typing) of Serratia marcescens isolates from nosocomial episodes (63 nosocomial cutbreaks with 475 isolates, and 1208 sporadic cases) received in our laboratory during the period 1981-1991 was studied. The records for 1683 isolates from Spanish hospitals have been analyzed. In relation with the sporadic cases, the predominant types were serotype O6 (13.4%) and serotype O14 (11.4%); polyagglutinable strains accounted for 15.6%; in outbreaks, type O14 is clearly predominant (27.4%). Phage-typing was a good secondary marker, with a 87.9% of typability; the number of lytic patterns was very high, extended patterns (six or more phages) being the most frequent. We have studied the characteristics of S. marcescens isolates causing infections in the nosocomial environment in Spain.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"607-12"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Lost paradigms, Thomas S. Kuhn].","authors":"C Chica","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"641-6"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20051351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Brief history of the Spanish Society of Microbiology. VI. From 1991 to 1995].","authors":"C García Mendoza","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this sixth and final chapter of the brief history of the Spanish Society for Microbiology (SEM), the major activities carried out from 1991 to 1995 are described. During that period, the 14th and 15th SEM National Congresses took place in Saragossa (1993) and Madrid (1995), respectively. The 1995 Congress was also the occasion to celebrate the fiftieth anniversary of the SEM. During that period, three new Specialized Groups were created: Molecular Microbiology. Aquatic Microbiology, and Protozoology. All Specialized Groups held their own meetings. Courses on the introduction to research in microbiology, for undergraduate students, continued, as well as the Awards of the SEM for young researchers. During that period César Nombela was elected FEMS President, and Fernando Laborda was elected President of the Biodeterioration Society. The photographs of the Presidents and Secretaries of the SEM since its foundation until 1995 are included.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"621-30"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20051349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.","authors":"N I López, M J Pettinari, B S Méndez","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from samples containing 10(2) E. coli cells, either in saline solution or in river water.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"557-62"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cellular content of storage inclusions in purple sulfur bacteria determined by ultrathin sections.","authors":"I Esteve, J Mas, N Gaju, R Guerrero","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Phototrophic sulfur bacteria accumulate storage inclusions as a mechanism to adapt to several types of environmental stress. We compare the content of elemental sulfur and poly-beta-hydroxybutyrate (PHB) found in cultures growing under laboratory conditions with the content found in microorganisms in natural environments. Since natural communities are extremely heterogeneous in composition (they do not contain only phototrophic sulfur bacteria) and physiological state, it was not possible to apply conventional chemical analyses for the determination of the contents of sulfur and PHB. The study was performed by means of transmission electron microscopy, which turned out to be an excellent tool for this purpose. The results indicate that, in natural environments, cells have an extremely high content of storage inclusions, much higher than their laboratory grown counterparts, probably as a consequence of less favorable environmental conditions.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"563-70"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}