营养物对紫色固氮菌合成海藻酸盐的影响及所产海藻酸盐的表征。

Microbiologia (Madrid, Spain) Pub Date : 1996-12-01

S A Sabry, K M Ghanem, W A Sabra

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引用次数: 0

摘要

在分批培养中研究了营养物对黄氏固氮菌产藻酸盐的影响。当MgSO4.7H2O浓度为0.3 g/l时，细菌藻酸盐的生成量最大。0.4 g/l NaCl, 42 mg/l CaCl2.2H2O，。4mg /l KH2PO4, 16mg /l K2HPO4, 2.5 mg/l FeSO4.7H2O, 2.9 mg/l H3BO3, 2mg /l ZnSO4.7H2O, 2mg /l Na2MoO4.2H2O, 0.3 mg/l CuSO4.5H2O, 0.2 mg/l MnCl2.4H2O。天然添加剂和诱导剂对藻酸盐产量没有促进作用，但醋酸盐对藻酸盐产量有促进作用。纯海藻酸盐含灰分0.36%，蛋白质0.4%。它类似于藻酸盐，但它有一个额外的乙酰基。含69.5%的M-M块、27.5%的M-G块和3%的G-G块。

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Effect of nutrients on alginate synthesis in Azotobacter vinelandii and characterization of the produced alginate.

The role of nutrients on alginate production by Azotobacter vinelandii was studied in batch cultures. The largest amount of bacterial alginate was obtained in presence of: 0.3 g/l MgSO4.7H2O. 0.4 g/l NaCl, 42 mg/l CaCl2.2H2O,.4 mg/l KH2PO4, 16 mg/l K2HPO4, 2.5 mg/l FeSO4.7H2O, 2.9 mg/l H3BO3, 2 mg/l ZnSO4.7H2O, 2 mg/l Na2MoO4.2H2O, 0.3 mg/l CuSO4.5H2O, 0.2 mg/l MnCl2.4H2O. Alginate production was not enhanced by natural additives or inducing agents, except for acetate, which increased alginate yield. The pure alginate contained 0.36% ash and 0.4% protein. It is similar to algal alginate, but it has an extra acetyl group. It contains 69.5% M-M block, 27.5% M-G block and 3% G-G block.

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来源期刊

Microbiologia (Madrid, Spain)

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