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Microbiologia (Madrid, Spain)最新文献

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Transport of phosphate into vacuoles of Saccharomyces cerevisiae. 磷酸在酿酒酵母液泡中的转运。
Microbiologia (Madrid, Spain) Pub Date : 1997-03-01
T V Kulakovskaya, I S Kulaev
{"title":"Transport of phosphate into vacuoles of Saccharomyces cerevisiae.","authors":"T V Kulakovskaya,&nbsp;I S Kulaev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Isolated vacuoles of the yeast Saccharomyces cerevisiae accumulated orthophosphate at pH 6-7. This accumulation was inhibited by MgATP, and was insensitive to protonophores. Triton X-100 blocked this process. The accumulation increased linear by any phosphate concentrations employed (from 0.2 to 10 mM). It is proposed that phosphate is transported into yeast vacuoles via a channel transport system independent of the electrochemical proton gradient on the vacuolar membrane.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"13 1","pages":"71-4"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20055571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
alpha-Tubulin of Histriculus cavicola (Ciliophora; Hypotrichea). 豚鼠组织的α-微管蛋白。
Microbiologia (Madrid, Spain) Pub Date : 1997-03-01
P Pérez-Romero, E Villalobo, C Díaz-Ramos, P Calvo, F Santos-Rosa, A Torres
{"title":"alpha-Tubulin of Histriculus cavicola (Ciliophora; Hypotrichea).","authors":"P Pérez-Romero,&nbsp;E Villalobo,&nbsp;C Díaz-Ramos,&nbsp;P Calvo,&nbsp;F Santos-Rosa,&nbsp;A Torres","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An alpha-tubulin gene fragment amplified by PCR from the hypotrichous ciliate Histriculus cavicola has been sequenced. This fragment, 1,182 bp long, contains an in-frame \"stop\" codon (UAA), which in other hypotrichous species codes for a glutamine residue. The comparison of the alpha-tubulin genes from several ciliates classes have revealed amino acid positions which could serve to distinguish these taxonomic groups.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"13 1","pages":"57-66"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20055569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The genus Rhodosporidium: a potential source of beta-carotene. 红孢子虫属:β -胡萝卜素的潜在来源。
Microbiologia (Madrid, Spain) Pub Date : 1997-03-01
T de Miguel, P Calo, A Díaz, T G Villa
{"title":"The genus Rhodosporidium: a potential source of beta-carotene.","authors":"T de Miguel,&nbsp;P Calo,&nbsp;A Díaz,&nbsp;T G Villa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Four wild-type species of the genus Rhodosporidium have been studied as as possible sources for the industrial production of beta-carotene. HPLC-based studies showed that their carotenoid composition consisted of almost pure beta-carotene at concentrations ranging from 226 to 685 micrograms/g of dried yeast biomass. These results are consistent with those obtained by spectrophotometry at 480 nm.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"13 1","pages":"67-70"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20055570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Jacobo Cárdenas (1940-1996): a friend]. [雅各布·卡德纳斯（1940-1996）：一个朋友]。
Microbiologia (Madrid, Spain) Pub Date : 1997-03-01
I Núñez de Castro
{"title":"[Jacobo Cárdenas (1940-1996): a friend].","authors":"I Núñez de Castro","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"13 1","pages":"83-4"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20079720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineers and microbiologists: a future together in bioremediation. 工程师和微生物学家:生物修复的共同未来。
Microbiologia (Madrid, Spain) Pub Date : 1997-03-01
X Maymó-Gatell, G Schraa
{"title":"Engineers and microbiologists: a future together in bioremediation.","authors":"X Maymó-Gatell,&nbsp;G Schraa","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"13 1","pages":"85-7"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20055573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Midwife to the greens: the electron capture detector. 绿党的助产士:电子捕获探测器。
Microbiologia (Madrid, Spain) Pub Date : 1997-03-01
J E Lovelock
{"title":"Midwife to the greens: the electron capture detector.","authors":"J E Lovelock","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>James E. Lovelock makes an account of the path he has followed since he started his scientific research at the National Institute for Medical Research (NIMR) in London in the 1940s, emphasizing the aspects related to environmental sciences. Lovelock explains the origins of the electron capture detector (ECD). So far, the ECD is the most sensitive, easily portable and inexpensive analytical apparatus capable of detecting substances present in the atmosphere at concentrations as low as parts per trillion (10(-12)). It has been the first device specifically sensitive to pollutants, and its use has provided the grounds for the development of environmental sciences, and green politics. The data gathered by the ECD about the persistence of pesticides in the environment led American biologist Rachel Carson to write her seminal book Silent Spring; data regarding the global presence of chlorofluorocarbons (CFC) in the atmosphere led Sherwood Rowland and Mario Molina to develop their theory of ozone depletion. The results of his research over the years led Lovelock himself to the development of the Gaia theory.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"13 1","pages":"11-22"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20055652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A dynamic state of a closed ecosystem and its significance to the generation of the Earth's environment. 封闭生态系统的动态状态及其对地球环境形成的意义。
Microbiologia (Madrid, Spain) Pub Date : 1997-03-01
S Moriyama, M Takahara
{"title":"A dynamic state of a closed ecosystem and its significance to the generation of the Earth's environment.","authors":"S Moriyama,&nbsp;M Takahara","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Earth forms a living-system composed of large materially closed ecosystems (CES). We are performing various experiments to study comparable living-systems, especially smaller samples we call \"microcosmic type CES\". CES have an ability to persist despite perturbation. We show experimental results of the behavior of CES mainly during the transition form a semi-open state to an entirely closed one. We report phenomena on CES instability which may be inherent in them. We suggest that CES possess some properties that may help elucidate the nature of the Earth as a living system. Climatic change on the Earth, such as oceanic anoxia and its consequences, may have been due to disturbances as modeled in a CES.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"13 1","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20055654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure and composition of freshwater microbial mats from a sulfur spring ("Font Pudosa", NE Spain). 西班牙东北部“Font Pudosa”硫磺泉淡水微生物席的结构和组成。
Microbiologia (Madrid, Spain) Pub Date : 1997-03-01
A Martínez, I Pibernat, J Figueras, J García-Gil
{"title":"Structure and composition of freshwater microbial mats from a sulfur spring (\"Font Pudosa\", NE Spain).","authors":"A Martínez,&nbsp;I Pibernat,&nbsp;J Figueras,&nbsp;J García-Gil","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Different types of microbial mats developing on the wall on a non-thermal sulfur freshwater spring have been studied. Both, light and electron microscopy as well as HPLC analysis of photosynthetic pigments revealed their structure and composition. Prokaryotic chlorophylls and carotenoids helped in the taxonomical assignment of the main photosynthetic groups. \"Inverted position\" mats (Mat-I) were dominated by Chromatiaceae; they were located closed to the water outlets (0.3 mM sulfide). \"Normal position\", that is, cyanobacterial-covered mats (Mat-II and Mat-IV), developed elsewhere on the stone walls at lower sulfide concentrations. A third type of mat (Mat-III), covered by chemolithotrophic bacteria, was distinguishable at the water-air interface, strongly attached to the walls of the spring. Up to six physiological types of microorganisms have been recognized: cyanobacteria, Chromatiaceae, purple nonsulfur bacteria. Chlorobiaceae, Chloroflexaceae, and chemolithotrophic bacteria. Cyanobacteria Lyngbya-like, Oscillatoria-like and Pseudanabaena sp. were found. The diversity of Chromatiaceae (six morpho-/pigment types of the genus Chromatium, plus two non identified Chromatiaceae, named PB1 and PB2 were observed) was noticeable. Chemolithotrophic bacteria were represented by the genera Beggiatoa and Thiothrix. Finally, small numbers of Chloroflexus-like bacteria and Chlorobium limicola were found in all the studied mats.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"13 1","pages":"45-56"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20055568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Challenge to microbiology in Latin America: a cholera epidemics at the end of the 20th century]. [拉丁美洲微生物学面临的挑战:20世纪末的霍乱流行]。
Microbiologia (Madrid, Spain) Pub Date : 1996-12-01
R R Colwell
{"title":"[Challenge to microbiology in Latin America: a cholera epidemics at the end of the 20th century].","authors":"R R Colwell","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"519-22"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20051348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Basic or applied research?]. [基础研究还是应用研究?]
Microbiologia (Madrid, Spain) Pub Date : 1996-12-01
J Olivares
{"title":"[Basic or applied research?].","authors":"J Olivares","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 4","pages":"661-2"},"PeriodicalIF":0.0,"publicationDate":"1996-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20051355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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