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Microbiologia (Madrid, Spain)最新文献

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Detection of enterohaemorrhagic Escherichia coli O157:H7 in minced beef using immunomagnetic separation. 免疫磁分离法检测肉糜中肠出血性大肠杆菌O157:H7。
Microbiologia (Madrid, Spain) Pub Date : 1996-09-01
J E Blanco, M Blanco, A Mora, C Prado, M Río, L Fernández, M J Fernández, V Sáinz, J Blanco
{"title":"Detection of enterohaemorrhagic Escherichia coli O157:H7 in minced beef using immunomagnetic separation.","authors":"J E Blanco,&nbsp;M Blanco,&nbsp;A Mora,&nbsp;C Prado,&nbsp;M Río,&nbsp;L Fernández,&nbsp;M J Fernández,&nbsp;V Sáinz,&nbsp;J Blanco","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 has been recently recognized as a human pathogen associated with haemorrhagic colitis and haemolytic uraemic syndrome. Most outbreaks of haemorrhagic colitis resulted from the consumption of undercooked minced beef or raw milk. Dairy cattle have been identified as a reservoir of EHEC O157: H7. In this study E. coli O157 specific antibody, coated on magnetic beads, was used to concentrate and release EHEC O157:H7 from meat samples. A survey of retail fresh minced beef and hamburger samples using this procedure revealed that 3 (5%) of 58 beef samples were positive for EHEC O157:H7. Two of the strains produced both VT1 and VT2 verotoxins, and one produced only VT2. Immunomagnetic separation is a sensitive and simple technique for the isolation of E. coli O157 from food, and could be useful for a further elucidation of the epidemiology of this organism. The relatively high prevalence of EHEC O157:H7 in beef samples may constitute a risk for public health. Thus, a suitable epidemiologic control and effective methods of prevention should be applied.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 3","pages":"385-94"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19862270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Institutions supporting microbiology in Cuba]. [古巴支持微生物学的机构]。
Microbiologia (Madrid, Spain) Pub Date : 1996-09-01
H O Halvorson
{"title":"[Institutions supporting microbiology in Cuba].","authors":"H O Halvorson","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 3","pages":"343-6"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20051343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Brief history of the Spanish Society of Microbiology. V. From 1987 to 1991]. [西班牙微生物学会简史]V.从1987年到1991年]。
Microbiologia (Madrid, Spain) Pub Date : 1996-09-01
C García Mendoza
{"title":"[Brief history of the Spanish Society of Microbiology. V. From 1987 to 1991].","authors":"C García Mendoza","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this fifth chapter of the short history of the Spanish Society for Microbiology (SEM), the major activities carried out from 1987 to 1991 are described. During that period, the 11th, 12th and 13th SEM National Congresses took place in Gijón (1987), Pamplona (1989) and Salamanca (1991), respectively. The Specialized Groups of the Society organized their own meetings. Courses on the introduction to research in microbiology, for undergraduate students, started in that period. The President of SEM was elected FEMS Vice-president during the Council Meeting of the Federation of European Microbiological Societies (FEMS), held in Madrid in September 1989.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 3","pages":"457-64"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20051344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Candida albicans gene expressed in Saccharomyces cerevisiae results in a distinct pattern of mRNA processing. 在酿酒酵母中表达的白色念珠菌基因导致mRNA加工的独特模式。
Microbiologia (Madrid, Spain) Pub Date : 1996-09-01
A Iborra, R Sentandreu, D Gozalbo
{"title":"A Candida albicans gene expressed in Saccharomyces cerevisiae results in a distinct pattern of mRNA processing.","authors":"A Iborra,&nbsp;R Sentandreu,&nbsp;D Gozalbo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two plasmids (derived from YCplac22 and YEplac112) carrying a Candida albicans gene (including the 5' non-coding promoter sequences) coding for a 30 kDa membrane-bound protein, were used to transform Saccharomyces cerevisiae cells. A 30 kDa protein was immunodetected by Western blot in the membrane fraction of transformants. Northern analysis showed the presence of three mRNA species (of about 1.1, 0.7 and 0.5 kb) hybridizing with the C. albicans gene as a probe. The same result was obtained using the 5' and 3' regions of the gene as probes, whereas only a 1.1 kb mRNA was found in C. albicans and none was detected in S. cerevisiae control transformants. Thus, heterologous expression of this gene in S. cerevisiae results in a distinct pattern of mRNA processing, either due to the location on plasmid vectors and/or to differences in the mRNA processing systems in the two microorganisms.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 3","pages":"443-8"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19862179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Growth of the fish pathogen Renibacterium salmoninarum on different media. 鱼致病菌沙门氏菌在不同培养基上的生长。
Microbiologia (Madrid, Spain) Pub Date : 1996-09-01
I Bandín, Y Santos, J I Barja, A E Toranzo
{"title":"Growth of the fish pathogen Renibacterium salmoninarum on different media.","authors":"I Bandín,&nbsp;Y Santos,&nbsp;J I Barja,&nbsp;A E Toranzo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the present study, the ability of a group of Renibacterium salmoninarum strains to grow in the presence or absence of the amino acid cysteine and other mineral and organic sources of sulfur and nitrogen has been evaluated. Most of the isolates tested were able to grow on a mineral media supplemented with L-cysteine-HCl or other organic compounds, such as the vitamin thiamine and a casein hydrolysate (Bacto Casamino Acids, Difco). Bacterial growth was also recorded on commercial and specific media not supplemented with L-cysteine-HCl, or in which this amino acid was replaced by the compounds cited above.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 3","pages":"439-42"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19862178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of heavy metals on soil denitrification and CO2 production after short term incubation. 短期孵育后重金属对土壤反硝化和CO2产生的影响。
Microbiologia (Madrid, Spain) Pub Date : 1996-09-01
A Probanza, F J Gutiérrez Mañero, B Ramos, N Acero, J A Lucas
{"title":"Effect of heavy metals on soil denitrification and CO2 production after short term incubation.","authors":"A Probanza,&nbsp;F J Gutiérrez Mañero,&nbsp;B Ramos,&nbsp;N Acero,&nbsp;J A Lucas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The toxicity of three heavy metals, Cd, Zn and Cu, has been tested in a Mediterranean soil. The soil was incubated (108h) with mixed solutions of those metals before evaluating denitrification and CO2 production, both by gas chromatography. These activities were used as biological indicators of heavy metal toxicity, and compared to non-treated control soil samples. Statistical analyses showed no significant differences in CO2 production between treated and non-treated control soils. The lowest levels of respiration were observed in soils treated with the largest amounts of Zn and Cd. Denitrification increased significantly in soils treated with solutions containing 100 micrograms/ml of Cu and 1000 micrograms/ml of Cd or Zn.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 3","pages":"417-24"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19862274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[José Luis Cánovas Palacio-Valdés (1934-1995)]. [何塞·路易斯·卡诺瓦斯·帕拉西奥-巴尔德斯（1934-1995年）]。
Microbiologia (Madrid, Spain) Pub Date : 1996-09-01
G Giménez Martín
{"title":"[José Luis Cánovas Palacio-Valdés (1934-1995)].","authors":"G Giménez Martín","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 3","pages":"465-8"},"PeriodicalIF":0.0,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20051345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Science in Latin America]. [拉丁美洲的科学]。
Microbiologia (Madrid, Spain) Pub Date : 1996-06-01
F J Ayala
{"title":"[Science in Latin America].","authors":"F J Ayala","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 2","pages":"163-6"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19739311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Virulence factors of the swine pathogen Actinobacillus pleuropneumoniae. 猪胸膜肺炎放线杆菌的毒力因素分析。
Microbiologia (Madrid, Spain) Pub Date : 1996-06-01
R I Tascón, J A Vázquez-Boland, C B Gutiérrez-Martín, J I Rodríguez-Barbosa, E F Rodríguez-Ferri
{"title":"Virulence factors of the swine pathogen Actinobacillus pleuropneumoniae.","authors":"R I Tascón,&nbsp;J A Vázquez-Boland,&nbsp;C B Gutiérrez-Martín,&nbsp;J I Rodríguez-Barbosa,&nbsp;E F Rodríguez-Ferri","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Gram-negative bacterium Actinobacillus pleuropneumoniae is the etiologic agent of swine pleuropneumonia, a highly contagious respiratory infection with great economic implications. In recent years, considerable efforts have been invested in the study of its virulence mechanisms. Here we review the current knowledge on the determinants of A. pleuropneumoniae pathogenicity, paying particular attention to the capsule, the lypopolysaccharide, the outer membrane proteins, and the RTX exotoxins. The contribution of other factors is also discussed.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 2","pages":"171-84"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19739313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The molecular mechanisms of actin-based intracellular motility by Listeria monocytogenes. 单核增生李斯特菌肌动蛋白胞内运动的分子机制。
Microbiologia (Madrid, Spain) Pub Date : 1996-06-01
T Chakraborty
{"title":"The molecular mechanisms of actin-based intracellular motility by Listeria monocytogenes.","authors":"T Chakraborty","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A key virulence trait of bacteria and viruses that multiply in the cytoplasm of the infected cell is their ability to direct movement intracellularly and to spread from cell-to-cell. Intracellular movement is effected by harnessing components of the host microfilament system. This mode of locomotion by intracytoplasmic parasites has recently gained much interest as a model to examine microfilament assembly and function. Of the intracellular bacteria employing association with the host cytoskeleton to effect movement, the Gram-positive pathogen Listeria monocytogenes is the most well studied. This review summarizes the current state of the understanding, at the molecular level, of how L. monocytogenes subverts the host cell contractile machinery to meet its own need to move and spread within infected host cells.</p>","PeriodicalId":77263,"journal":{"name":"Microbiologia (Madrid, Spain)","volume":"12 2","pages":"237-44"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19741219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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