用PCR扩增法检测天然水样中加入的多磷酸激酶基因。

Microbiologia (Madrid, Spain) Pub Date : 1996-12-01

N I López, M J Pettinari, B S Méndez

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引用次数: 0

摘要

建立了一种快速、简便的大肠杆菌多磷酸激酶(ppk)基因PCR扩增检测方法。该方法使用过滤器从样品中回收细胞，这使得它适合于环境研究。在含10(2)个大肠杆菌细胞的样品中，分别在盐水溶液或河水中检测ppk基因。

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Monitoring by PCR amplification of the polyphosphate kinase gene added to natural water samples.

A fast, simple method for the detection of the Escherichia coli polyphosphate kinase (ppk) gene by means of PCR amplification is described. The method uses filters to recover cells from the samples, which makes it suitable for environmental studies. The detection of the ppk gene was achieved from samples containing 10(2) E. coli cells, either in saline solution or in river water.

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Microbiologia (Madrid, Spain)

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