Aerobiologia最新文献

{"title":"On-site monitoring of airborne pathogens: recent advances in bioaerosol collection and rapid detection","authors":"Xiaorui Feng,&nbsp;Peiya Hu,&nbsp;Tingyu Jin,&nbsp;Jiehong Fang,&nbsp;Fan Tang,&nbsp;Han Jiang,&nbsp;Chenze Lu","doi":"10.1007/s10453-024-09824-y","DOIUrl":"10.1007/s10453-024-09824-y","url":null,"abstract":"<div><p>Airborne pathogens pose a great threat to public health, and their appearance in bioaerosol also increases the contiguousness due to the long survival time and transmitting range. Real-time monitoring and rapid detection methods provide more effective prevention and control of airborne pathogens. The whole procedure could be divided into bioaerosol collection and detection processes. This review presents the basic principles and recent advances in commonly used methods for each of these two steps. We categorized four different kinds of collection methods based on their principles and discussed possible enrichment methods against a small amount of targets. Four different detection methods were compared regarding their ability to perform rapid testing. In the final section, we analyzed the latest trend in combining all these steps to set up a single device or platform for rapid, automated, and continuous on-site bioaerosol monitoring to overcome time and space constraints and increase the speed of the entire monitoring process. We conclude that an integrated all-in-one system using a microfluidic platform is the most promising solution for real-time monitoring of airborne pathogens, since they are capable of simplifying operational steps, efficient collection, and high-throughput detection, demonstrating the strong potential of field-deployable platforms.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 3","pages":"303 - 341"},"PeriodicalIF":2.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140934027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyses of culturable microorganisms and chemical pollutants in the air of urban and rural areas in the region of São Paulo, Brazil","authors":"Dulcilena de Matos Castro e Silva,&nbsp;Valter Batista Duo Filho,&nbsp;Rosa Maria Nascimento Marcusso,&nbsp;Maria Regina Alves Cardoso,&nbsp;Fábio Luiz Teixeira Gonçalves","doi":"10.1007/s10453-024-09823-z","DOIUrl":"10.1007/s10453-024-09823-z","url":null,"abstract":"<div><p>Bioaerosols are particles of great importance for several fields of research, and spores produced by fungi can exist as bioaerosols when suspended in the air. Microbiological standards for environmental monitoring of outdoor air parameters can be achieved by analyzing the relationship between airborne microorganisms and the prevailing environmental conditions. The outdoor air of the Metropolitan Region of São Paulo and the rural area in a city of the state of São Paulo (Ibiúna/SP), both in Brazil, were evaluated for the presence of microorganisms using the MAS-100 ECO (Merck®, Fr.) and M Air T (Millipore®) air sample collectors. Dichloran Rose-Bengal Chloramphenicol and Tryptic Soy Agars were used for fungal and bacterial isolation, respectively. Bacterial colonies were counted, and the plates with fungal colonies were sent for phenotypic identification up to genus and species level, respectively. Data on pollutant concentrations were obtained from the Environmental Company of the State of São Paulo. The highest number of Colony-Forming Units/m³ (CFU/m³) of microorganisms was measured in the winter and summer seasons, respectively, but the greatest Spore-Forming Units (SFU) of fungi were found in the rural area, where pollutant concentrations were lower. Nitrogen dioxide (NO₂) had a slightly positive influence on the concentration of SFU of fungi in both areas studied. Sulfur dioxide (SO₂) pollutant concentrations had both positive and negative great relations showing influence on microbial counts in the air of the rural area. In the rural area, the low bacteria count was influenced negatively by the low concentration of carbon monoxide (CO). The microbial counts were related to each other, as well as to the concentrations of pollutants, shown by all the correlations seen, indicating microorganisms as biomarkers of pollution in outdoor areas. The influence of environmental factors on the population and outdoor air biome is also explicit.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 3","pages":"403 - 414"},"PeriodicalIF":2.2,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140934299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ragweed in South America: the relevance of aerobiology stations in Latin America","authors":"Ivan Cherrez-Ojeda,&nbsp;Karla Robles-Velasco,&nbsp;German D. Ramon,&nbsp;Laura Barrionuevo,&nbsp;Oscar Calderon Llosa,&nbsp;Denisse Cevallos-Levicek,&nbsp;Marco Faytong-Haro,&nbsp;Andrés Espinoza-Maticurena,&nbsp;Patricio Alvarez-Muñoz,&nbsp;Ivan Tinoco,&nbsp;László Makra,&nbsp;Áron József Deák","doi":"10.1007/s10453-024-09825-x","DOIUrl":"10.1007/s10453-024-09825-x","url":null,"abstract":"<div><p>Pollen, particularly from the <i>Ambrosia</i> genus, plays a pivotal role in triggering allergic rhinoconjunctivitis symptoms. This review delves into the global background of <i>Ambrosia</i>, focusing on its origins, invasive potential, and spread to South America. The ecological niche for <i>Ambrosia</i> species is explored, emphasizing its stability globally but exhibiting unique and dynamic features in South America. Information on <i>Ambrosia</i> pollen concentration in South America is summarized, revealing varying levels across countries. The establishment of new aerobiology stations, as highlighted in the latest findings, contributes valuable data for understanding allergen risk management in the region. The health perspective addresses the rise in allergic diseases due to climate change, emphasizing the need for continuous monitoring, especially in South America. Agricultural damage inflicted by <i>Ambrosia</i> is discussed, emphasizing its invasive potential, high seed production, and negative impact on crops, forage quality, and livestock. The review also positions <i>Ambrosia</i> as a marker of climate change, discussing the effects of global warming on pollen seasons, concentrations, and allergenic characteristics. The importance of expanding aerobiology stations in South America is underscored, requiring collaborative efforts from government, scientific societies, and academic institutions. The review concludes by advocating for increased monitoring to address potential challenges posed by <i>Ambrosia</i>, offering a basis for tailored interventions and future research in South American regions.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 3","pages":"343 - 351"},"PeriodicalIF":2.2,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10453-024-09825-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140934414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: AutoPollen special issue","authors":"Fiona Tummon,&nbsp;Bernard Clot","doi":"10.1007/s10453-024-09821-1","DOIUrl":"10.1007/s10453-024-09821-1","url":null,"abstract":"","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 1","pages":"1 - 2"},"PeriodicalIF":2.2,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140657412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing uncertainty in airborne birch pollen modelling","authors":"Willem W. Verstraeten,&nbsp;Rostislav Kouznetsov,&nbsp;Nicolas Bruffaerts,&nbsp;Mikhail Sofiev,&nbsp;Andy W. Delcloo","doi":"10.1007/s10453-024-09818-w","DOIUrl":"10.1007/s10453-024-09818-w","url":null,"abstract":"<div><p>In Europe, more than one quarter of the adult population and one third of the children suffer from pollinosis, but the geographical variability is large. In Belgium, at least ~ 10% of the people develop allergies due to birch pollen. These patients may benefit from a forecasting system that raises alerts when episodes with huge amount of airborne birch pollen grains are expected. Such a forecast system for birch pollen was established for the Belgian territory in 2023 based on the pollen emission and transport model System for Integrated modeLling of Atmospheric coMposition (SILAM). The question, however, is which uncertainty in modelling and forecasting airborne pollen levels can be expected? Here, we assess the uncertainty in modelling airborne birch pollen levels near the surface using SILAM in a Monte Carlo error approach summarized by the relative Coefficient of Variation (CV%) as descriptive statistic for the season of 2018 in Belgium. For the major inputs that drive the birch pollen model—the amount and location of birch trees (0.1° × 0.1° map), the start and end of the birch pollen season (1° × 1° map), and the ripening temperature of birch catkins—sets of 100 randomly sampled data layers were prepared for running SILAM 100 times. For each set of model input, 100 spatio-temporal maps of airborne birch pollen levels were produced and its spread was quantified by the CV%. We show that the spatial uncertainty of pollen emissions sources in SILAM is substantially high, but that the uncertainties of the parameters determining the start and end of the season are at least equally important. By accumulating the effects of all investigated model input uncertainties including the impact of the catkins-ripening temperature, CV% values of 50% and more are obtained when quantifying the variation of the modelled airborne birch pollen levels. These errors are in line with reported values from the current reference method for monitoring airborne birch pollen grains near the surface.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 2","pages":"271 - 286"},"PeriodicalIF":2.2,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140617608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial variation of airborne bacterial heterogeneity and potential opportunistic human pathogens: a comparative study of sites in Korea and Japan","authors":"Makoto Seki,&nbsp;Hitoshi Tanaka,&nbsp;Shinichi Yonemochi,&nbsp;Ki-Ho Lee,&nbsp;Young-Ju Kim,&nbsp;Reika Iwamoto,&nbsp;Kei Sato,&nbsp;Daisuke Tanaka","doi":"10.1007/s10453-024-09817-x","DOIUrl":"10.1007/s10453-024-09817-x","url":null,"abstract":"<div><p>Biological particles known as bioaerosols are present in the atmosphere and have recently been implicated as influencing agriculture, cloud development, biogeography, and human health. The present study was conducted to characterize airborne bacterial heterogeneity at Jeju Island in Korea and at Saitama and Toyama in Japan, focusing on potential human pathogens. Air samples were collected during the winter, when the monsoon blows from the northwest. Samples were analyzed by high-throughput sequencing and denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA genes to detect spatial differences in airborne bacteria and the possible spread of bacteria by transboundary transport. Compositions of the bacterial in samples collected on the same dates from the different sites were similar. Notably, bacteria from two genera that are potentially pathogenic for humans—<i>Acinetobacter</i> and <i>Clostridium</i>—were detected on the same day in both Korea and Japan. These results indicate the possibility of long-range transport of airborne bacteria and its potential impact on human health.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 2","pages":"287 - 295"},"PeriodicalIF":2.2,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140582498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culturable bacteria in clouds at Réunion, tropical island","authors":"Thomas Charpentier,&nbsp;Muriel Joly,&nbsp;Céline Judon,&nbsp;Martine Sancelme,&nbsp;Magali Abrantes,&nbsp;Mickaël Vaïtilingom,&nbsp;Christelle Ghaffar,&nbsp;Maxence Brissy,&nbsp;Maud Leriche,&nbsp;Anne-Marie Delort,&nbsp;Laurent Deguillaume,&nbsp;Pierre Amato","doi":"10.1007/s10453-024-09819-9","DOIUrl":"10.1007/s10453-024-09819-9","url":null,"abstract":"<div><p>The viable bacterial assemblages in clouds at Réunion Island (Indian Ocean) were examined through culture-based approach. A total of 176 isolates were recovered from 15 independent cloud events collected during 3 field campaigns, and identified to the species level through full length 16S rRNA gene sequencing. As often in atmospheric samples, Alpha-, Beta- and Gamma-proteobacteria dominated, along with Actinobacteria, Firmicutes, and Bacteroidetes, depicting these as the backbone of the global aeromicrobiome. A comparative analysis with similar work from a cloud sampling site in Central France revealed site-specificities, and numerous common species. These latter included members of <i>Pseudomonas</i>, <i>Sphingomonas</i>, <i>Bacillus</i>, <i>Staphylococcus</i>, <i>Rhodococcus</i> and others, whose such widespread presence in clouds supports the existence of a pan-atmospheric microbiome. This also confirms that cultures remain powerful methods in the description of the viable microbial diversity by allowing deep taxonomic affiliation.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 2","pages":"297 - 302"},"PeriodicalIF":2.2,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140582378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of automatic pollen and fungal spore monitoring across major end-user domains","authors":"Fiona Tummon,&nbsp;Beverley Adams-Groom,&nbsp;Célia M. Antunes,&nbsp;Nicolas Bruffaerts,&nbsp;Jeroen Buters,&nbsp;Paloma Cariñanos,&nbsp;Sevcan Celenk,&nbsp;Marie Choël,&nbsp;Bernard Clot,&nbsp;Antonella Cristofori,&nbsp;Benoît Crouzy,&nbsp;Athanasios Damialis,&nbsp;Alberto Rodríguez Fernández,&nbsp;Délia Fernández González,&nbsp;Carmen Galán,&nbsp;Björn Gedda,&nbsp;Regula Gehrig,&nbsp;Monica Gonzalez-Alonso,&nbsp;Elena Gottardini,&nbsp;Jules Gros-Daillon,&nbsp;Lenka Hajkova,&nbsp;David O’Connor,&nbsp;Pia Östensson,&nbsp;Jose Oteros,&nbsp;Andreas Pauling,&nbsp;Rosa Pérez-Badia,&nbsp;Victoria Rodinkova,&nbsp;F. Javier Rodríguez-Rajo,&nbsp;Helena Ribeiro,&nbsp;Ingrida Sauliene,&nbsp;Branko Sikoparija,&nbsp;Carsten Ambelas Skjøth,&nbsp;Antonio Spanu,&nbsp;Mikhail Sofiev,&nbsp;Olga Sozinova,&nbsp;Lidija Srnec,&nbsp;Nicolas Visez,&nbsp;Letty A. de Weger","doi":"10.1007/s10453-024-09820-2","DOIUrl":"10.1007/s10453-024-09820-2","url":null,"abstract":"<div><p>The advent of automatic pollen and fungal spore monitoring over the past few years has brought about a paradigm change. The provision of real-time information at high temporal resolution opens the door to a wide range of improvements in terms of the products and services made available to a widening range of end-users and stakeholders. As technology and methods mature, it is essential to properly quantify the impact automatic monitoring has on the different end-user domains to better understand the real long-term benefits to society. In this paper, we focus the main domains where such impacts are expected, using Europe as a basis to provide qualitative estimates and to describe research needs to better quantify impacts in future. This will, in part, also serve to justify further investment and help to expand monitoring networks.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 1","pages":"57 - 75"},"PeriodicalIF":2.2,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10453-024-09820-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140582435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial analysis of COVID-19 risk factors: a case study in Bangladesh","authors":"Sefat-E- Barket,&nbsp;Md. Rezaul Karim","doi":"10.1007/s10453-024-09815-z","DOIUrl":"10.1007/s10453-024-09815-z","url":null,"abstract":"<div><p>In 2019, the world grappled with an unexpected and severe global health crisis—the Coronavirus disease (COVID-19) outbreak, which significantly impacted various aspects of human life. This case study, focusing on Bangladesh, aimed to uncover the complex spatial patterns and potential risk factors influencing the virus’s uneven spread across 64 districts. To analyze spatial patterns, two techniques, namely Moran <i>I</i> and Geary <i>C</i>, were employed to study spatial autocorrelation. Hotspots and coldspots were identified using local Moran <i>I</i>, while spatial hotspots were pinpointed using local Getis Ord <i>G</i>. Exploring spatial heterogeneity involved implementing two non-spatial models (Poisson–Gamma and Poisson-Lognormal) and three spatial models (Conditional Autoregressive model, Convolution model, and Leroux model) through Gibbs sampling. The Leroux model emerged as the optimal choice, meeting criteria based on the lowest values of deviance information criterion and Watanabe–Akaike information criterion. Regression analysis revealed that factors such as humidity, population density, and urbanization were associated with an increase in COVID-19 cases, while the aging index appeared to hinder the virus’s spread. The research outcomes provide a comprehensive framework adaptable to the evolving nature of COVID-19 in Bangladesh. It categorizes influential factors into distinct clusters, enabling government agencies, policymakers, and healthcare professionals to make informed decisions for controlling the pandemic and addressing future infectious diseases.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 2","pages":"247 - 269"},"PeriodicalIF":2.2,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140315353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and health risk assessment of size-segregated fungal bioaerosols in and around a sugar mill in India","authors":"Swati Tyagi,&nbsp;Arun Srivastava","doi":"10.1007/s10453-024-09812-2","DOIUrl":"10.1007/s10453-024-09812-2","url":null,"abstract":"<div><p>Suspended particles of biological origin comprising of virus, fragments of plants and animals dander, pollen grains, fungal spores and bacteria known as bioaerosols have become a major concern in the past decades. In the present study reports, the concentration and size distribution of fungal bioaerosol in and around a sugar mill situated in the Muzaffarnagar region of Uttar Pradesh, India, are presented. The sampling was performed in the winter when the mill used to be in the operational mode. The highest mean fungal concentration was observed at the cutter site (4022 ± 321 cfu/m³) and lowest at storage site (832 ± 85 cfu/m³). The maximum and minimum concentration of fungal bioaerosol was observed during January (3090 ± 174 cfu/m³⁾ and March (629 ± 69 cfu/m³) respectively. During the entire sampling period, the fine fraction of fungal bioaerosol was observed to be significantly higher at all the sites, whereas coarse fraction was lower. The association between fine and coarse fractions of bioaerosols showed a very strong positive relationship. The levels of fungal bioaerosol and their association with the meteorological parameters in sugar mill were also conducted. A positive association with the relative humidity and wind speed was observed at significance level of <i>p</i> &lt; 0.05, whereas a negative relation was observed with temperature at <i>p</i> &lt; 0.05. The lifetime average daily dose was calculated for both inhalation and dermal; among them LADD_inhalation is ~ 5 times over LADD_dermal. The health risk index was observed as &lt; 1 for both inhalation and dermal routes, whereas HI_inhalation value was 10⁵ times higher than the HI_dermal value. The dominant fungi genera found in the air of examined dwellings were <i>Penicillium</i> spp., <i>Aspergillus</i> spp., <i>Cladosporium</i> spp., and <i>Alternaria</i> spp., which occurred predominantly at all of the studied sites during the sampling period.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"40 2","pages":"201 - 215"},"PeriodicalIF":2.2,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140204598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}