In vitro cellular & developmental biology : journal of the Tissue Culture Association最新文献_第7页

Morphologic study of angiogenesis in vitro. 体外血管生成的形态学研究。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630933

T Ishibashi, K Sueishi, T Murata, H Inomata

引用次数: 1

A cobblestone cell isolated from the human omentum: the mesothelial cell; isolation, identification, and growth characteristics. 从人网膜中分离出来的鹅卵石细胞:间皮细胞;分离、鉴定和生长特性。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630943

A Pronk, P Leguit, A A Hoynck van Papendrecht, E Hagelen, T J van Vroonhoven, H A Verbrugh

{"title":"A cobblestone cell isolated from the human omentum: the mesothelial cell; isolation, identification, and growth characteristics.","authors":"A Pronk, P Leguit, A A Hoynck van Papendrecht, E Hagelen, T J van Vroonhoven, H A Verbrugh","doi":"10.1007/BF02630943","DOIUrl":"https://doi.org/10.1007/BF02630943","url":null,"abstract":"Normal human mesothelial cells (NHMC) were isolated from pieces of human omentum. The cell yield was approximately one million cells per square centimeter omentum. The mesothelial cells were identified by their positive staining with monoclonal antibodies against cytokeratins 6 and 18. Transmission electronmicroscopy of cultured NHMC revealed many microvilli on the apical surface and many mitochondria and pinocytotic vesicles in the cytoplasm, indicating active transmembrane transport. Growth of NHMC was directly related to the concentration of human serum or of fetal bovine serum in the growth medium. Addition of epidermal growth factor with or without hydrocortisone resulted in a significant increase of NHMC growth; when endothelial cell growth factor, insulin, or hydrocortisone were added no such increase was observed. Seeding NHMC at densities less than 3000/cm2 did not result in monolayer formation. The mesothelial cells were serially passed in growth medium M199 with added 10% fetal bovine serum up to 7 passages. However, after Passage 4 the cells changed into giant cells with an irregular pattern, and a lack of intracellular cytokeratin expression was observed for most of the cells.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 2","pages":"127-34"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02630943","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18685805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Differences in growth of neurons from normal and regenerated teleost spinal cord in vitro. 正常和再生硬骨骨脊髓神经元体外生长的差异。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630946

M J Anderson

引用次数: 7

Polarized secretion of tissue-plasminogen activator in cultured thyroid cells. 组织纤溶酶原激活剂在培养甲状腺细胞中的极化分泌。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630948

S Desruisseau-Gonzalvez, P Delori, D Gruffat, O Chabaud

{"title":"Polarized secretion of tissue-plasminogen activator in cultured thyroid cells.","authors":"S Desruisseau-Gonzalvez, P Delori, D Gruffat, O Chabaud","doi":"10.1007/BF02630948","DOIUrl":"https://doi.org/10.1007/BF02630948","url":null,"abstract":"We studied the polarized secretion of tissue-type plasminogen activator in porcine thyroid cells cultured as a monolayer on porous bottom chambers. The presence of tissue-type plasminogen activator was detected by zymographic analysis on two independent media that were in contact either with the apical surface or with the basolateral membrane. The amount of tissue-type plasminogen activator was determined in both media by ELISA and enzyme assay. Measurable tissue-type plasminogen activator activity was found in the basal but not in the apical medium. However, on zymogram, a lytic zone corresponding to tissue-type plasminogen activator was visible in both media. In addition, a lytic band at 130 kDa suggested presence of a complex formed by tissue-type plasminogen activator and an inhibitor. Preferential basolateral tissue-type plasminogen activator antigen secretion (70%) has been observed, showing the possible relation between tissue-type plasminogen activator and extracellular matrix components. Neither tissue-type plasminogen activator level nor polarized secretion seemed to be regulated by thyrotropin (0.1 mU/ml).","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 2","pages":"161-4"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02630948","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19454597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Endothelial proliferation and adhesivity. 内皮细胞的增殖和粘附。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630932

I J Udeinya

引用次数: 0

Transient promoter activity in primary rat mammary epithelial cells evaluated using particle bombardment gene transfer. 用粒子轰击基因转移评价大鼠乳腺上皮细胞瞬时启动子活性。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630949

T A Thompson, M N Gould, J K Burkholder, N S Yang

{"title":"Transient promoter activity in primary rat mammary epithelial cells evaluated using particle bombardment gene transfer.","authors":"T A Thompson, M N Gould, J K Burkholder, N S Yang","doi":"10.1007/BF02630949","DOIUrl":"https://doi.org/10.1007/BF02630949","url":null,"abstract":"The relative strengths of several commonly used viral promoters in primary cultures of rat mammary epithelial cells were studied using a particle bombardment gene transfer method. NIH 3T3 cells were also examined as a representative cell line. Initially, the conditions necessary for efficient gene transfer using particle bombardment were determined. Discharge voltage for particle bombardment was evaluated to maximize the levels of gene expression and cell viability. After transfection, transgene expression decreased over a 5-day period in both mammary cells and NIH 3T3 cells. Particle bombardment gene transfer was at least fivefold more efficient than lipofection, calcium phosphate co-precipitation, or electroporation. The activity of five viral enhancer/promoters was compared using a luciferase gene assay system. The relative promoter strengths in mammary cells were determined to be: RSV approximately CMV approximately SV40 > MLV > MMTV. Tissue-specific activity of the MMTV-LTR was demonstrated, although this promoter conferred the lowest expression level among the promoters tested.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 2","pages":"165-70"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02630949","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19372037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

In vitro stimulation of human endothelial cells by derivatized dextrans. 衍生右旋糖酐对人内皮细胞的体外刺激。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-01-01 DOI: 10.1007/BF02634373

D Letourneur, J Champion, F Slaoui, J Jozefonvicz

{"title":"In vitro stimulation of human endothelial cells by derivatized dextrans.","authors":"D Letourneur, J Champion, F Slaoui, J Jozefonvicz","doi":"10.1007/BF02634373","DOIUrl":"https://doi.org/10.1007/BF02634373","url":null,"abstract":"Derivatized dextrans exert a stimulatory effect on the in vitro growth of human umbilical vein endothelial cells (HUVEC). Measurements of growth were monitored by [3H]thymidine uptake and cell numbers. Our results show that some derivatized dextrans at 4 micrograms/ml (88 nM) increase the [3H]thymidine incorporation, whereas starting dextran (40,000 Da), dextran sulfate, and carboxymethyl dextran have no effect. In addition, heparin under similar experimental conditions shows a slight inhibitory effect on the HUVEC growth. The stimulatory effect of derivatized dextrans was also found when HUVEC grew during 7 days in medium containing 2% fetal bovine serum. We also observed that derivatized dextrans had no effect on the mitogenic activity of acidic fibroblast growth factor, a mitogenic factor for several cell types including HUVEC. By assessment of [3H]thymidine uptake at 48 h without serum, we concluded that the exogenous growth factors were not involved in the proliferative activity of these components. The stimulatory effects are related to the chemical nature and the proportion of substituents on the synthetic polysaccharides. The data indicate that benzylamide sulfonated groups play a key role in the stimulation of HUVEC growth. Neither carboxyl nor sulfate groups alone exhibit this effect. Thus, the stimulatory capacity of dextran derivatives depends strongly on the respective ratios of the functional groups.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 1","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634373","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18683274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Growth of normal human ovarian surface epithelial cells in reduced-serum and serum-free media. 正常人卵巢表面上皮细胞在低血清和无血清培养基中的生长。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-01-01 DOI: 10.1007/BF02634366

W M Elliott, N Auersperg

{"title":"Growth of normal human ovarian surface epithelial cells in reduced-serum and serum-free media.","authors":"W M Elliott, N Auersperg","doi":"10.1007/BF02634366","DOIUrl":"https://doi.org/10.1007/BF02634366","url":null,"abstract":"The human ovarian surface epithelium (OSE) is believed responsible for over 85% of ovarian cancers, yet little is known about the normal biology of these cells. To date, culture of OSE has only been reported in media with high serum supplements. We have developed two media, one with less than 1% of serum (OSEM-1) and the other comprised of highly purified and defined materials (OSEM-2), which allow us to study OSE under relatively defined conditions. By substituting 0.05% of Pedersen's fetuin for 15% fetal bovine serum (FBS) with Medium 199/MCDB105 basal medium, the cell numbers reached 50 to 60% of those in the presence of 15% FBS over 7 days. However, over several weeks, the total number of population doublings achieved were comparable to those in 15% FBS. Addition of insulin, transferrin, ethanolamine, lipoic acid, and phosphatidylcholine to the medium with Pedersen's fetuin (OSEM-1) enhanced growth up to 20% more than in their absence. Supplementation of M199/105 with highly purified (> 99%) fetuin, alpha 2-macroglobulin, and hydrocortisone resulted in a defined medium (OSEM-2) that permitted 1 to 2 doublings/7 days. In addition, cells maintained a more normal, epithelial-like morphology in culture for a longer period in the presence of Pedersen's or purified fetuin than in M199/105/15% FBS, thus increasing the number of morphologically normal cells available for experimentation. Addition of 0.05% Pedersen's fetuin to M199/105 in the presence of 6 to 8% FBS resulted in levels of growth equivalent to those in M199/105/15% FBS alone. We are now able to study the effects of various compounds on the growth and differentiation of OSE under defined conditions, and have reduced the requirement for FBS to produce large numbers of OSE cells.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 1","pages":"9-18"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634366","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18683275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Transient induction of cytochrome P450 1A1 mRNA by culture medium component in primary cultures of adult rat hepatocytes. 培养基成分对成年大鼠肝细胞原代培养细胞色素P450 1A1 mRNA的瞬时诱导作用。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-01-01 DOI: 10.1007/BF02634372

T A Kocarek, E G Schuetz, P S Guzelian

{"title":"Transient induction of cytochrome P450 1A1 mRNA by culture medium component in primary cultures of adult rat hepatocytes.","authors":"T A Kocarek, E G Schuetz, P S Guzelian","doi":"10.1007/BF02634372","DOIUrl":"https://doi.org/10.1007/BF02634372","url":null,"abstract":"Because the metabolic environment can alter gene expression in cultured cells, we examined the effects of change of medium on the levels of several cytochrome P450 mRNAs in primary cultures of rat hepatocytes maintained on Matrigel. The amounts of P450 1A2, 2B1/2, or 3A1 mRNA were unaffected by changing the medium. In contrast, P450 1A1 mRNA levels were increased 1 to 2 h after media change, reached maximum levels by 6 h, and declined to baseline by 24 h. Supplementing day-old media with components of the medium revealed that only addition of amino acids resulted in 1A1 mRNA induction. From the results of direct additions and omissions, we showed that tryptophan, but not histidine, was largely responsible for the 1A1 mRNA induction. Moreover, mild photoactivation of the tryptophan resulted in a substantially increased magnitude of 1A1 mRNA induction. The time course for 1A1 mRNA induction by treatment with photoactivated tryptophan was identical to that observed after medium change. Treatment of hepatocyte cultures with beta-naphthoflavone, which is metabolized by 1A1, also resulted in a transient 1A1 mRNA induction time-course profile over a 24-h period, whereas treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin, which is relatively stable to metabolic transformation, produced sustained elevations of 1A1 mRNA, suggesting that the transient response to tryptophan also may involve metabolism of the inducer. Our results extend previous data showing that oxidized products of tryptophan induce 1A1, and suggest that the transient nature of the induction may be due to elimination of the activated tryptophan molecule.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 1","pages":"62-6"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634372","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19428999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Interstitial cells from the atrial and ventricular sides of the bovine mitral valve respond differently to denuding endocardial injury. 来自牛二尖瓣心房侧和心室侧的间质细胞对剥脱性心内膜损伤的反应不同。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-01-01 DOI: 10.1007/BF02634370

W M Lester, A A Damji, I Gedeon, M Tanaka

{"title":"Interstitial cells from the atrial and ventricular sides of the bovine mitral valve respond differently to denuding endocardial injury.","authors":"W M Lester, A A Damji, I Gedeon, M Tanaka","doi":"10.1007/BF02634370","DOIUrl":"https://doi.org/10.1007/BF02634370","url":null,"abstract":"The mitral valve has atrial and ventricular sides, each lined by endocardial cells. The valve stroma contains alpha smooth muscle actin positive interstitial cells, collagen, glycosaminoglycans, and elastic tissue. To eliminate the effect of endocardium on wound repair in bovine mitral valve organ culture, the endocardium was removed from both sides of the valve. At 6 days, organ cultures of these preparations revealed surface cells on the ventricular side but not in the atrial side. Ventricular surface cells were negative for Factor VIII-related antigen, and positive for alpha smooth muscle actin. Immunoperoxidase staining for proliferating cell nuclear antigen/cyclin, a marker for cell proliferation, revealed a positive labeling index of (mean +/- standard deviation) 0.08 +/- 0.16% for interstitial cells from the atrial side and 0.14 +/- 0.19% for ventricular side interstitial cells in uncultured preparations (not significant), and 0.44 +/- 0.69% for atrial side interstitial cells and 2.25 +/- 1.64% for ventricular side interstitial cells in the cultured preparations (significant, P < 0.0006). The results suggest that in organ culture, interstitial cells from the ventricular side of the mitral valve respond to a denuding endocardial injury by proliferating and migrating onto the adjacent surface whereas interstitial cells from the atrial side do not. This difference in the response to injury of interstitial cells from the atrial and ventricular sides of the valve may reflect differences in phenotype or may be due to effects of extracellular matrix on interstitial cell behavior. The latter is possible because of differences in the extracellular matrix of the atrial and ventricular sides of the valve.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 1","pages":"41-50"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634370","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19088053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31