In vitro cellular & developmental biology : journal of the Tissue Culture Association最新文献_第10页

New human ovarian cell line OVCCR1/sf in serum-free medium. 无血清培养基中新的人卵巢细胞系OVCCR1/sf。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631049

S Jozan, H Roché, F Cheutin, M Carton, B Salles

引用次数: 1

GAPDH acts as an inducible not constitutive gene in cultured endothelial cells. 在培养的内皮细胞中，GAPDH是一种可诱导的非构成基因。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631056

J A Rimarachin, J Norcross, P Szabo, B B Weksler

引用次数: 2

Use of ciprofloxacin and BM-Cyclin in mycoplasma decontamination. 环丙沙星和BM-Cyclin在支原体去污中的应用。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631057

C Somasundaram, W Nicklas, S Matzku

引用次数: 4

Relative promoter activity in human mammary epithelial cells assayed by transient expression. 用瞬时表达法测定人乳腺上皮细胞的相对启动子活性。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631061

G Huper, J R Marks, J R Wiener, J D Iglehart

{"title":"Relative promoter activity in human mammary epithelial cells assayed by transient expression.","authors":"G Huper, J R Marks, J R Wiener, J D Iglehart","doi":"10.1007/BF02631061","DOIUrl":"https://doi.org/10.1007/BF02631061","url":null,"abstract":"Chimeric DNA expression vectors containing regulatory sequences proximal to the 5' end of coding sequences for mammalian genes provide valuable tools to study gene expression. Genes coding for easily measured products (reporter genes) can be used to study promoter strength and regulation of gene expression after transient expression of promoter-reporter constructs in mammalian cells. To determine the strength of a variety of mammalian and viral promoter-enhancer sequences in primary cultures of human mammary epithelial cells (HMEC), these sequences were fused to the bacterial chloramphenicol acetyltransferase (CAT) gene and transfected into HMEC using strontium phosphate. The long terminal repeat (LTR) of the endogenous murine leukemia virus AKR-623 was the most potent promoter of transient CAT expression in HMEC. A number of commonly available promoter sequences displayed a wide range of activities in these cells. The glucocorticoid responsive LTR promoter from the murine mammary tumor virus modulated expression of CAT and was sensitive to the concentration of dexamethasone in the growth media. In a similar fashion, the regulatory sequences from the murine metallothionein-1 gene retained responsiveness to zinc concentration in the growth media.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"28A 11-12","pages":"730-4"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02631061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12654593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sebaceous cells in monolayer culture. 单层培养的皮脂腺细胞。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631053

C C Zouboulis

引用次数: 7

Cell-surface glycoconjugate diversity among lepidopteran cell lines. 鳞翅目细胞系间细胞表面糖结合物的多样性。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631054

W J McCarthy, K A Fletcher

引用次数: 0

Evidence supporting the role of a proteinaceous, loosely bound extracellular molecule in the cell density signaling between tendon cells. 支持蛋白性松散结合的细胞外分子在肌腱细胞之间的细胞密度信号传导中的作用的证据。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631063

J R Zayas, R I Schwarz

{"title":"Evidence supporting the role of a proteinaceous, loosely bound extracellular molecule in the cell density signaling between tendon cells.","authors":"J R Zayas, R I Schwarz","doi":"10.1007/BF02631063","DOIUrl":"https://doi.org/10.1007/BF02631063","url":null,"abstract":"Normal cells in culture respond to cell density by altering their proliferation rates and their pattern of protein expression. Primary avian tendon (PAT) cells are a case in point where procollagen production increases approximately 10-fold at high cell density while proliferation almost ceases. In an earlier report focusing on the cell density regulation of procollagen expression, the signaling mechanism communicating the presence of other cells was shown to have the characteristics of a loosely bound component of the cell layer. Extending these studies to the cell density regulation of proliferation, the cell density signal (CDS) was again shown to be altered by medium agitation, stimulating cell division. Agitation, however, was only disruptive to cell signaling when there was a high ratio of medium to cells. When sufficient cells were present, agitation was less effective. Therefore, the CDS controlling procollagen production and the CDS controlling the inhibition of growth seemed to be linked because the signaling mechanism is disrupted in a parallel manner by agitation. However, the proliferative response of PAT cells is more complex in that there is also a positive influence at moderate cell density (> 2 x 10(4) cells/cm2) on the rate of cell division. As a consequence, PAT cells would not proliferate into an area of low cell density, but within the same dish would rapidly fill an area of moderate density. PAT cells were capable of filling a gap between high cell density areas if the gap was less than 2 mm. Medium agitation also affected cells at low cell density in a different manner. It was inhibitory if all the cells were at low cell density but it was stimulatory if the cells at low cell density were in close proximity to cells at high cell density. In addition, medium conditioned by agitation over cells at a high cell density would stimulate cells at low cell density to divide and grow out into low cell density regions. Using the growth-promoting activity of the conditioned medium as an assay, this component of the CDS was shown to have unique characteristics: heat, pH, dithiothreitol (DTT) stable; tris ion and protease sensitive. By gel exclusion chromatography it was larger than 100 kDa. But after DTT treatment its mobility shifted to < 30 kDa while retaining activity.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"28A 11-12","pages":"745-54"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02631063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12654594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Polymorphonuclear leukocytes-mediated lysis of A431 cells induced by IgG1 mouse anti-epidermal growth factor receptor monoclonal antibodies. IgG1小鼠抗表皮生长因子受体单克隆抗体诱导多态核白细胞介导的A431细胞裂解。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631068

T Kawamoto, K Kishimoto, K Takahashi, T Matsumura, J D Sato, S Taniguchi

{"title":"Polymorphonuclear leukocytes-mediated lysis of A431 cells induced by IgG1 mouse anti-epidermal growth factor receptor monoclonal antibodies.","authors":"T Kawamoto, K Kishimoto, K Takahashi, T Matsumura, J D Sato, S Taniguchi","doi":"10.1007/BF02631068","DOIUrl":"https://doi.org/10.1007/BF02631068","url":null,"abstract":"The ability of different Fc receptors (Fc gamma R) on IgG-mediated cytotoxicity for human epidermoid carcinoma A431 cells bearing large number of epidermal growth factor receptors (EGFRs) was examined by using two isotypes (IgG1 and IgG2a) of murine monoclonal antibodies (MoAbs) against EGFR in the presence of human monocytes and granulocytes. Two MoAbs (225 and LA1) of the IgG1 isotype exhibited effective cytolytic activity for A431 cells with human polymorphonuclear leukocytes (PMN) rather than with human mononuclear cells (MNC). In contrast, two MoAbs (528 and 579) of the IgG2a-isotype lysed the cells less effectively with PMN than with MNC. Anti-Fc gamma R II (CDw32) MoAb 2E1 inhibited the IgG1-mediated cytotoxicity by PMN, and anti-Fc gamma R III (CD16) MoAb 80H3 did not inhibit the IgG2a-mediated cytotoxicity by MNC. Under these conditions, antibody-dependent cell-mediated cytotoxicity by mouse MoAb IgG1 isotypes resulted from antibody binding to the Fc gamma R II (CDw32) of PMN.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"28A 11-12","pages":"782-6"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02631068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12654598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Participation of the mitochondrial genome in the differentiation of neuroblastoma cells. 线粒体基因组参与神经母细胞瘤细胞的分化。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631065

J L Vayssière, L Cordeau-Lossouarn, J C Larcher, M Basseville, F Gros, B Croizat

{"title":"Participation of the mitochondrial genome in the differentiation of neuroblastoma cells.","authors":"J L Vayssière, L Cordeau-Lossouarn, J C Larcher, M Basseville, F Gros, B Croizat","doi":"10.1007/BF02631065","DOIUrl":"https://doi.org/10.1007/BF02631065","url":null,"abstract":"Using clonal cell lines isolated from murine neuroblastoma C1300, we investigated the mitochondrial changes related to neuronal differentiation and, more generally, the role played by the mitochondrion in this phenomenon. By different approaches (measurement of the mitochondrial mass, immunoquantification of specific mitochondrial proteins, or incorporation of Rhodamine 123), the differentiation of the inducible clone, N1E-115, was found associated with an important increase of the cellular content in mitochondria. This increase could be observed with differentiating N1E-115 cells maintained in suspension, i.e. under conditions where neurite outgrowth is prevented but other early stages of (biochemical) differentiation continue to occur. That these mitochondrial changes are likely to be correlated with these stages of neuronal differentiation, rather than with simple progression to the postmitotic stage, stems from comparative experiments with clone N1A-103, a neuroblastoma cell line variant that becomes postmitotic after induction but fails to differentiate and shows no modification in its cellular content in mitochondria. In accordance with these observations, chloramphenicol prevents differentiation when added together with the inducer. This effect is probably related to the inhibition of mitochondrial translation rather than to modification of the bioenergetic needs because oligomycine, a potent inhibitor of the mitochondrial ATP synthetase, shows no effect on neurogenesis. As a working hypothesis and in keeping with independently published models, we postulate that products resulting from mitochondrial translation could be involved in the organization of the cytoskeleton or of certain membrane components whose rearrangements should be the prerequisite or the correlates to early stages of neuronal differentiation.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"28A 11-12","pages":"763-72"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02631065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12654595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 78

Criteria that optimize the potential of murine embryonic stem cells for in vitro and in vivo developmental studies. 优化小鼠胚胎干细胞体外和体内发育研究潜力的标准。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1992-11-01 DOI: 10.1007/BF02631066

D G Brown, M A Willington, I Findlay, A L Muggleton-Harris

{"title":"Criteria that optimize the potential of murine embryonic stem cells for in vitro and in vivo developmental studies.","authors":"D G Brown, M A Willington, I Findlay, A L Muggleton-Harris","doi":"10.1007/BF02631066","DOIUrl":"https://doi.org/10.1007/BF02631066","url":null,"abstract":"Cultured mouse embryonic stem (ES) cells are used for both in vitro and in vivo studies. The uncommitted pluripotent cells provide a model system with which to study cellular differentiation and development; they can also be used as vectors to carry specific mutations into the mouse genome by homologous recombination. To ensure successful integration into the germ line, competent totipotent diploid ES cell lines are selected using a cell injection bioassay that is both time consuming and technically demanding. The prolonged in vitro culture of rapidly dividing ES cells can lead to accumulated changes and chromosomal abnormalities that will compromise the biological function and abrogate germ line transmission of chimeric mice carrying novel genetic mutations. Such in vitro conditions will vary between individual laboratories; for example, differences in the serums used for maintenance. Using a number of different criteria we attempt in this paper to define the parameters that we found to be key factors for optimization of the biological potential of established ES cell lines. The successful integration into the germ line is dependant on acquiring or deriving a competent totipotent mouse ES diploid cell line. In this paper parameters and criteria are defined which we found to be key factors for the optimization of the biological potential of established ES cell lines.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"28A 11-12","pages":"773-8"},"PeriodicalIF":0.0,"publicationDate":"1992-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02631066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12654599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11