In vitro cellular & developmental biology : journal of the Tissue Culture Association最新文献

{"title":"program schedule","authors":"Shelley S Noland, R. Spinner, J. Freeman","doi":"10.1007/BF02623556","DOIUrl":"https://doi.org/10.1007/BF02623556","url":null,"abstract":"","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"24 1","pages":"1A-11A"},"PeriodicalIF":0.0,"publicationDate":"2021-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02623556","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42455944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid-stemmed spring wheat cultivars give better androgenic response than hollow-stemmed cultivars in anther culture","authors":"D. Weigt, A. Kiel, J. Nawracała, M. Pluta, A. Łacka","doi":"10.1007/s11627-016-9793-2","DOIUrl":"https://doi.org/10.1007/s11627-016-9793-2","url":null,"abstract":"","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"52 1","pages":"619 - 625"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11627-016-9793-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52693344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conversion of oat (Avena sativa L.) haploid embryos into plants in relation to embryo developmental stage and regeneration media","authors":"A. Noga, E. Skrzypek, M. Warchoł, I. Czyczyło-Mysza, K. Dziurka, I. Marcińska, K. Juzoń, T. Warzecha, A. Sutkowska, Z. Nita, Krystyna Werwińska","doi":"10.1007/s11627-016-9788-z","DOIUrl":"https://doi.org/10.1007/s11627-016-9788-z","url":null,"abstract":"","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"52 1","pages":"590 - 597"},"PeriodicalIF":0.0,"publicationDate":"2016-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11627-016-9788-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52693331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability of adenine-based cytokinins in aqueous solution.","authors":"David S Hart, Andrew Keightley, Daryl Sappington, Phuong T M Nguyen, Charleen Chritton, Gary R Seckinger, Kenneth C Torres","doi":"10.1007/s11627-015-9734-5","DOIUrl":"10.1007/s11627-015-9734-5","url":null,"abstract":"<p><p>Since the isolation of the first cytokinin almost 60 yr ago, cytokinins have become critically important for ornamental and agricultural crops in plant tissue culture. Despite the extensive research on this class of compounds, little information is available on the chemical stability of cytokinins in solution or following an autoclave cycle with Murashige and Skoog (MS) basal medium. This work describes the stability in aqueous solutions of five widely used adenine-based cytokinins: trans-zeatin (tZ), 6-(γ,γ-dimethylallylamino) purine (2iP), kinetin, benzyladenine (BA), and <i>m</i>-topolin. High pressure liquid chromatography (HPLC) and electrospray ionization-mass spectrometry (ESI-MS) were used to quantify and identify their degradation. BA, kinetin, 2iP, and <i>m</i>-topolin were stable at 1.0 mg mL^-1 in 0.05 N KOH, with no statistically significant concentration changes (<i>p</i> > 0.05) after 90 d of storage at temperatures of -20°C, 2-6°C, or 25°C. The cytokinin tZ was used as a model compound to evaluate stability under alkaline and acid conditions as well as after repeated freeze-thaw cycles. Trans-zeatin retained >90% of the initial concentration of 1.0 mg mL^-1 when dissolved in 0.01 N KOH and stored at -20°C and 2-6°C for 90 d, with only the 2-6°C temperature treatment showing a statistical significant concentration change (<i>p</i> = 0.03). The 1.0 mg mL^-1 tZ solution in 0.01 N KOH was stable through six repeated freeze-thaw cycles over 90 d without any significant change in concentration compared to the initial freeze-thaw. Yet, tZ showed highly significant concentration changes when dissolved at 50 mg mL^-1 and 0.5 N KOH. All of these adenine-based cytokinins showed exceptional stability following an autoclave cycle at 121°C, 110 kPa for 30 min when in solutions of 1.0 mg mL^-1 in 0.05 N KOH, with no significant degradation detected. Trans-zeatin was also found to be stable after one autoclave cycle with 1× MS-basal salts.</p>","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"52 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52693269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> propagation and assessment of genetic stability of acclimated plantlets of <i>Cornus alba</i> L. using RAPD and ISSR markers.","authors":"Agnieszka Ilczuk, Ewelina Jacygrad","doi":"10.1007/s11627-016-9781-6","DOIUrl":"10.1007/s11627-016-9781-6","url":null,"abstract":"<p><p><i>Cornus alba</i> L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegetative propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of <i>C. alba</i> cultivars 'Aurea' and 'Elegantissima' were cultured on media enriched with six different sources of macronutrients. Media were supplemented with either <i>N</i>⁶-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L^-1 BA, 0.1 mg L^-1 NAA, and 20-30 g L^-1 sucrose. Rooting of regenerated shoots was achieved by an <i>in vitro</i> method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L^-1 NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for 'Aurea' and 90% for 'Elegantissima'. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymorphic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197-199 and 184-187 distinct and reproducible band classes, respectively, in 'Aurea' and 'Elegantissima' plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant.</p>","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"52 1","pages":"379-390"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52693318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low <i>Agrobacterium tumefaciens</i> inoculum levels and a long co-culture period lead to reduced plant defense responses and increase transgenic shoot production of sunflower (<i>Helianthus annuus</i> L.).","authors":"Zhifen Zhang, John J Finer","doi":"10.1007/s11627-016-9774-5","DOIUrl":"10.1007/s11627-016-9774-5","url":null,"abstract":"<p><p><i>Agrobacterium</i>-mediated plant transformation is typically conducted by inoculating plant tissues with an <i>Agrobacterium</i> suspension containing approximately 10⁸-10⁹ bacteria mL^-1, followed by a 2-3-d co-culture period. Use of longer co-culture periods could potentially increase transformation efficiencies by allowing more time for <i>Agrobacterium</i> to interact with plant cells, but bacterial overgrowth is likely to occur, leading to severe tissue browning and reduced transformation and regeneration. Low bacterial inoculum levels were therefore evaluated as a means to reduce the negative outcomes associated with long co-culture. The use of low inoculum bacterial suspensions (approximately 6 × 10² bacteria mL^-1) followed by long co-culture (15 d) led to the production of an average of three transformed sunflower shoots per explant while the use of high inoculum (approximately 6 × 10⁸ bacteria mL^-1) followed by short co-culture (3 d) led to no transformed shoots. Low inoculum and long co-culture acted synergistically, and both were required for the improvement of sunflower transformation. Gene expression analysis via qRT-PCR showed that genes related to plant defense response were generally expressed at lower levels in the explants treated with low inoculum than those treated with high inoculum during 15 d of co-culture, suggesting that low inoculum reduced the induction of plant defense responses. The use of low inoculum with long co-culture (LI/LC) led to large increases in sunflower transformation efficiency. This method has great potential for improving transformation efficiencies and expanding the types of target tissues amenable for transformation of different plant species.</p>","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"52 1","pages":"354-366"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52693299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of using biotechnology to improve cassava: a review.","authors":"Paul Chavarriaga-Aguirre, Alejandro Brand, Adriana Medina, Mónica Prías, Roosevelt Escobar, Juan Martinez, Paula Díaz, Camilo López, Willy M Roca, Joe Tohme","doi":"10.1007/s11627-016-9776-3","DOIUrl":"10.1007/s11627-016-9776-3","url":null,"abstract":"<p><p>The importance of cassava as the fourth largest source of calories in the world requires that contributions of biotechnology to improving this crop, advances and current challenges, be periodically reviewed. Plant biotechnology offers a wide range of opportunities that can help cassava become a better crop for a constantly changing world. We therefore review the state of knowledge on the current use of biotechnology applied to cassava cultivars and its implications for breeding the crop into the future. The history of the development of the first transgenic cassava plant serves as the basis to explore molecular aspects of somatic embryogenesis and friable embryogenic callus production. We analyze complex plant-pathogen interactions to profit from such knowledge to help cassava fight bacterial diseases and look at candidate genes possibly involved in resistance to viruses and whiteflies-the two most important traits of cassava. The review also covers the analyses of main achievements in transgenic-mediated nutritional improvement and mass production of healthy plants by tissue culture and synthetic seeds. Finally, the perspectives of using genome editing and the challenges associated to climate change for further improving the crop are discussed. During the last 30 yr, great advances have been made in cassava using biotechnology, but they need to scale out of the proof of concept to the fields of cassava growers.</p>","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"52 1","pages":"461-478"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5071364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"52693312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annual Meeting Abstracts","authors":"","doi":"10.1007/BF02623557","DOIUrl":"https://doi.org/10.1007/BF02623557","url":null,"abstract":"","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"24 1","pages":"12A-72A"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02623557","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51594984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inheritance of somatic embryogenesis and plantlet regeneration from primary (type 1) callus in maize","authors":"M. Willman, S. Schroll, T. Hodges","doi":"10.1007/BF02624417","DOIUrl":"https://doi.org/10.1007/BF02624417","url":null,"abstract":"","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"25 1","pages":"95-100"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02624417","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51598159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Procoagulant activity of mouse transformed cells: Different expression in freshly isolated or cultured cells","authors":"L. Curatolo, M. Alessio, B. Casali, A. Falanga, M. Donati, N. Semeraro","doi":"10.1007/BF02624604","DOIUrl":"https://doi.org/10.1007/BF02624604","url":null,"abstract":"","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"25 1","pages":"388"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02624604","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51598501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}