正常和再生硬骨骨脊髓神经元体外生长的差异。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI:10.1007/BF02630946

M J Anderson

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引用次数: 7

摘要

将硬骨鱼正常成年脊髓和再生脊髓的外植体和游离细胞在体外培养8 ~ 12周。在此期间，神经元显示出广泛的神经突生长。再生脊髓的组织外植体和游离细胞的神经突生长比正常(未再生)脊髓的神经突生长得更快，更丰富。使用黏附基质和高密度的外植体或游离细胞可以使神经突的生长最大化。由于无尾龙在受伤后能够自然再生脊髓，而哺乳动物则不能，因此该培养系统将有助于研究控制(允许)这种成年脊椎动物脊髓神经元再生的因素。

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Differences in growth of neurons from normal and regenerated teleost spinal cord in vitro.

Explants and dissociated cells from normal adult spinal cord and regenerating cord of the teleost Apteronotus albifrons were grown in vitro for periods of 8 to 12 wk. During this time the neurons showed extensive neurite outgrowth. Neurite outgrowth from tissue explants and dissociated cells of regenerated spinal cord starts sooner and is more profuse than that from normal (unregenerated) cord. Neurite outgrowth is maximized by using adhesive substrata and a high density of explants or dissociated cells. Inasmuch as Apteronotus does regenerate its spinal cord naturally after injury, whereas mammals do not, this culture system will be useful to study factors that control (permit) regeneration of spinal neurons in this adult vertebrate.

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In vitro cellular & developmental biology : journal of the Tissue Culture Association

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