Polarized secretion of tissue-plasminogen activator in cultured thyroid cells.

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI:10.1007/BF02630948

S Desruisseau-Gonzalvez, P Delori, D Gruffat, O Chabaud

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引用次数: 3

Abstract

We studied the polarized secretion of tissue-type plasminogen activator in porcine thyroid cells cultured as a monolayer on porous bottom chambers. The presence of tissue-type plasminogen activator was detected by zymographic analysis on two independent media that were in contact either with the apical surface or with the basolateral membrane. The amount of tissue-type plasminogen activator was determined in both media by ELISA and enzyme assay. Measurable tissue-type plasminogen activator activity was found in the basal but not in the apical medium. However, on zymogram, a lytic zone corresponding to tissue-type plasminogen activator was visible in both media. In addition, a lytic band at 130 kDa suggested presence of a complex formed by tissue-type plasminogen activator and an inhibitor. Preferential basolateral tissue-type plasminogen activator antigen secretion (70%) has been observed, showing the possible relation between tissue-type plasminogen activator and extracellular matrix components. Neither tissue-type plasminogen activator level nor polarized secretion seemed to be regulated by thyrotropin (0.1 mU/ml).

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组织纤溶酶原激活剂在培养甲状腺细胞中的极化分泌。

我们研究了组织型纤溶酶原激活剂在多孔底腔培养的猪甲状腺细胞中的极化分泌。组织型纤溶酶原激活剂的存在是通过酶谱分析检测两种独立的介质接触或与根尖表面或基底外膜。组织型纤溶酶原激活物在两种培养基中分别采用酶联免疫吸附试验和酶法测定。组织型纤溶酶原激活剂活性在基部培养基中可测，而在顶端培养基中无。然而，在酶谱上，在两种培养基中都可以看到与组织型纤溶酶原激活物相对应的溶解区。此外，在130 kDa处的裂解带表明存在由组织型纤溶酶原激活剂和抑制剂形成的复合物。观察到基底外侧组织型纤溶酶原激活物抗原的优先分泌(70%)，表明组织型纤溶酶原激活物与细胞外基质成分之间可能存在联系。组织型纤溶酶原激活物水平和极化分泌似乎都不受促甲状腺素(0.1 mU/ml)的调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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In vitro cellular & developmental biology : journal of the Tissue Culture Association

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