In vitro cellular & developmental biology : journal of the Tissue Culture Association最新文献_第6页

A novel human glioma cell line from pituitaries. 一种新的人类脑垂体胶质瘤细胞系。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630939

W N Wen, L Chang

引用次数: 0

TGF-beta gene expression depends on tissue architecture. tgf - β基因的表达取决于组织结构。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630937

D Theodorescu, C Sheehan, R S Kerbel

引用次数: 5

Insulin-like growth factor-II receptor expression in normal and N-methyl-N'-nitro-nitrosoguanidine exposed cell lines: assessment by flow cytometry. 胰岛素样生长因子- ii受体在正常和n-甲基-n '-硝基-亚硝基胍暴露细胞系中的表达:流式细胞术评估

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630940

W H Thornton, L Barnett, R S MacDonald

引用次数: 0

Density gradient separation of growth plate chondrocytes. 生长板软骨细胞密度梯度分离。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630941

D E Carey, M Alini, Y Matsui, A R Poole

引用次数: 4

Isolation and culture of rat superior mesenteric artery smooth muscle cells. 大鼠肠系膜上动脉平滑肌细胞的分离培养。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630944

P G McGuire, H M Walker-Caprioglio, S A Little, L J McGuffee

引用次数: 6

Retinoic acid modulation of alpha(1-->2) fucosyltransferase activity and sensitivity of tumor cells to LAK-mediated cytotoxicity. 维甲酸调节α（1-->2）岩藻糖基转移酶活性和肿瘤细胞对LAK介导的细胞毒性的敏感性

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630945

N Labarrière, J P Piau, R Zennadi, P Blanchardie, M Denis, P Lustenberger

引用次数: 4

A serum-free medium for human epidermal-like cells. 人表皮样细胞的无血清培养基。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630934

P T Iype, G D Stoner, B W Gabriel, M E Kaighn

引用次数: 1

Serum-free medium for long-term growth, freezing, cloning and fusion of myelomas and hybridomas. 用于骨髓瘤和杂交瘤长期生长、冷冻、克隆和融合的无血清培养基。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630931

J L Li, Y J Li, S Chao, M H Ouyang, Y B Peng

引用次数: 0

"Normal" breast cells adjacent to a tumor grown in long-term three-dimensional culture. 在长期三维培养中生长的肿瘤旁的正常乳腺细胞。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630936

R Beaupain, C Mainguené, D Brouty-Boyé, P Planchon, V Magnien

引用次数: 1

Factors regulating the emergence of spontaneous and X-ray-induced variants in primary rat tracheal epithelial cell cultures. 调节原代大鼠气管上皮细胞培养中自发和x射线诱导变异出现的因素。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630942

M Terzaghi-Howe

{"title":"Factors regulating the emergence of spontaneous and X-ray-induced variants in primary rat tracheal epithelial cell cultures.","authors":"M Terzaghi-Howe","doi":"10.1007/BF02630942","DOIUrl":"https://doi.org/10.1007/BF02630942","url":null,"abstract":"<p><p>A series of experiments have been carried out to identify those factors that affect the number of altered populations detected in control, nonexposed, and radiation-exposed primary cultures of rat tracheal epithelial cells. The number of colony forming cells per milliliter of culture medium and the frequency with which the culture medium is changed seemed to be the most critical factors regulating the emergence of induced and spontaneous variants. Increasing the number of cells plated so that of colony forming cells increase from 25 to 200 per ml, regardless of the dish size used, was associated with a 200-fold decline in the frequency of spontaneous variants and a 40-fold decline in X-ray-induced variants. Increasing the interval between medium changes from 3 to 7 days after the first week of culture was associated with a 10-fold decrease in the frequency of spontaneous variants. The frequency of spontaneous and induced variants is markedly less dependent on culture density at densities between 150 and 600 colony forming cells per ml. The type of medium used to establish primary cultures had little effect on the frequency of variants detected. Similarly, when assays were performed at densities in excess of 150 colony forming cells per ml the frequency of spontaneous and x-ray-induced variants was not affected by the absence of epidermal growth factor, increased levels of calcium (final concentration, 0.8 mM), or by removal of pyruvate from the selection medium.</p>","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 2","pages":"120-6"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02630942","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19456524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3