In vitro cellular & developmental biology : journal of the Tissue Culture Association最新文献_第5页

Liposomes can specifically target entrapped melanin to hair follicles in histocultured skin. 脂质体可以专门针对组织培养皮肤中被困黑色素的毛囊。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634181

L Li, V K Lishko, R M Hoffman

引用次数: 25

Characterization of the DiFi rectal carcinoma cell line derived from a familial adenomatous polyposis patient. 家族性腺瘤性息肉病患者DiFi直肠癌细胞系的鉴定。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634191

M Olive, S Untawale, R J Coffey, M J Siciliano, D M Wildrick, H Fritsche, S Pathak, L M Cherry, M Blick, P Lointier

{"title":"Characterization of the DiFi rectal carcinoma cell line derived from a familial adenomatous polyposis patient.","authors":"M Olive, S Untawale, R J Coffey, M J Siciliano, D M Wildrick, H Fritsche, S Pathak, L M Cherry, M Blick, P Lointier","doi":"10.1007/BF02634191","DOIUrl":"https://doi.org/10.1007/BF02634191","url":null,"abstract":"The DiFi human colorectal cancer cell line was recently established from a familial adenomatous polyposis patient with extracolonic features characteristic of the Gardner syndrome. These cells have now been propagated for 150 passages in standard culture media and vessels without feeder layers or collagen coatings. They retain features of colonic epithelial cells such as surface microvilli, secretory vesicles, and desmosomes. Cytosol of DiFi cells contains a high level (502 U/mg protein) of the mucin CA 19-9. In addition, DiFi cells produce carcinoembryonic antigen, and induce tumors in athymic mice. Cytoskeleton analysis of DiFi cells by fluorescence microscopy showed a pronounced disorganization of actin cable structure. The isozyme genetic signature of DiFi cells is unique (0.01 probability of finding the same genetic signature in a different cell line), differs from that of HeLa cells, and has expressional features seen in other colorectal cell lines. The DiFi cell karyotype is tetraploid, contains many marker chromosomes, and shows numerous episomal particles. Two copies of chromosome 18 were absent, and only a single normal chromosome 17 was found. This parallels detection of allelic losses from DiFi cell DNA at loci on chromosomes 17p and 18 using molecular (cDNA) probes. DiFi cells clearly express transcripts for the c-myc proto-oncogene, the c-myb proto-oncogene, and the p53 tumor suppressor gene. Transforming growth factor beta inhibits DiFi cell growth in soft agar and suppresses c-myc expression in these cells. The value of this cell line in the study of genetic alterations in colorectal cancer is discussed.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 3 Pt 1","pages":"239-48"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634191","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19370949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Human endothelial cell line from an angiosarcoma. 来自血管肉瘤的人内皮细胞系。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634183

M L Hoover, V Vĕtvicka, J M Hoffpauir, C H Tamburro

引用次数: 9

Immortalization of human mammary epithelial cells by SV40 large T-antigen involves a two step mechanism. SV40大t抗原对人乳腺上皮细胞的永生化涉及两步机制。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634177

B A Van der Haegen, J W Shay

引用次数: 21

Mitogens in bovine pituitary for cultured rat tracheal cells. 牛垂体中有丝分裂原对培养大鼠气管细胞的影响。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634179

D G Thomassen

引用次数: 0

Synergistic inhibition of human rhabdomyosarcoma cells by sodium phenylacetate and Tretinoin. 苯乙酸钠和维甲酸对人横纹肌肉瘤细胞的协同抑制作用。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634180

G K Górski, L E McMorrow, M H Donaldson

引用次数: 2

Extracellular calcium does not contribute to cryopreservation-induced cytotoxicity. 细胞外钙与低温保存诱导的细胞毒性无关。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634185

L S Rhoads, A M Danks, J Im, A Warner, R L Isaacson, J Baust, R G Van Buskirk

{"title":"Extracellular calcium does not contribute to cryopreservation-induced cytotoxicity.","authors":"L S Rhoads, A M Danks, J Im, A Warner, R L Isaacson, J Baust, R G Van Buskirk","doi":"10.1007/BF02634185","DOIUrl":"https://doi.org/10.1007/BF02634185","url":null,"abstract":"The possible role of extracellular calcium ([Ca+2]e) in cryopreservation-induced cytotoxicity was tested using Madin-Darby canine kidney (MDCK) cells and a fluorescent multiple endpoint assay. MDCK cells maintained in 2 mM [Ca+2]e and treated with the calcium ionophore, ionomycin, increased their intracellular calcium ([Ca+2]i) as revealed by the calcium indicator dye, Fluo3 and the bottom-reading spectrofluorometer, CytoFluor 2300. The addition of 10 mM [ethylene bis (oxyethylenenitrilo)]-tetraacetic acid (EGTA) to the extracellular medium before treatment with ionomycin blocked this ionomycin-dependent increase in [Ca+2]i. A number of site and activity-specific fluorescent probes were surveyed to determine which indicator dye might best reveal the ionomycin-induced cytotoxic events during this increase in [Ca+2]i. Although most dyes changed their emission profiles in response to calcium, neutral red was found to best reflect the loss of [Ca+2]i homeostasis. The NR50 for a 15-min exposure to ionomycin in the presence of 2 mM [Ca+2]e was approximately 2 microM ionomycin, but ionomycin had little apparent effect on neutral red retention when 10 mM EGTA was added to the extracellular medium. Thus it was clear that an increase in [Ca+2]i could be cytotoxic to MDCK cells and that neutral red could monitor this cytotoxic episode. To test if [Ca+2]e was similarly cytotoxic during cryopreservation, MDCK cells were subjected to cryopreservation in the presence of dimethylsulfoxide (DMSO). In contrast to previous studies, plasma membrane integrity, not lysosomal function, seemed to best correlate with cell survival subsequent to cryopreservation. In addition, decreasing [Ca+2]e had no discernable effect on the retention of plasma membrane indicator dyes, neutral red, or cell survival. It is concluded that a) plasma membrane indicator dyes, not neutral red, might be better indicators of cytotoxicity occurring during cryopreservation; b) DMSO might be toxic to lysosomes during cryopreservation of cultured cells; and c) although [Ca+2]e can contribute to cytotoxicity, the presence of [Ca+2]e might not influence cryopreservation-induced cytotoxicity.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 3 Pt 1","pages":"208-14"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19446530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Fetal bovine serum and other sera used in tissue culture increase epithelial permeability. 用于组织培养的胎牛血清和其他血清增加上皮的通透性。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634190

K H Mortell, A D Marmorstein, E B Cramer

{"title":"Fetal bovine serum and other sera used in tissue culture increase epithelial permeability.","authors":"K H Mortell, A D Marmorstein, E B Cramer","doi":"10.1007/BF02634190","DOIUrl":"https://doi.org/10.1007/BF02634190","url":null,"abstract":"Fetal bovine serum (FBS) or heat-inactivated FBS (56 degrees C for 30 min, HFBS) caused a dose-dependent decrease in the transepithelial electrical resistance of an epithelial monolayer (MDCK). A saturating concentration of HFBS (30%) caused an average fall of 25 +/- 2% within 60 min. Upon removal of HFBS, the resistance returned to its starting value within 1 h. Flux studies with [3H]mannitol demonstrate that the fall in resistance is due to an increased permeability of the tight junctions. Thirty percent heat inactivated sera from goat, newborn calf, calf, bovine, and horse caused falls ranging from 26 to 47%. In contrast with the basolateral preference of human and bovine adult sera, fetal bovine and newborn calf sera elicit this response primarily by interacting with the apical surface of the epithelium. HFBS-treated monolayers show a significant increase in the condensation of F-actin at points where > or = 3 cells meet. These results demonstrate that FBS and other sera used as nutritional supplements can increase the permeability of the tight junctions of cultured epithelial cells.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 3 Pt 1","pages":"235-8"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634190","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19446534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

New model of vascular cell repair in vitro. 血管细胞体外修复新模型。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630938

V Kolpakov, A Di Sciullo, C Amore, M Nasuti, L Iacoviello, A Poggi, M B Donati

引用次数: 2

Production of autostimulatory growth factors by the human carcinoma line, RPMI 2650. 人癌细胞系RPMI 2650产生的自刺激生长因子。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-02-01 DOI: 10.1007/BF02630947

B M Carey, M Dooley, R Weedle, M Clynes

{"title":"Production of autostimulatory growth factors by the human carcinoma line, RPMI 2650.","authors":"B M Carey, M Dooley, R Weedle, M Clynes","doi":"10.1007/BF02630947","DOIUrl":"https://doi.org/10.1007/BF02630947","url":null,"abstract":"The human carcinoma line RPMI 2650 produces autocrine factors; they are detected by the ability of RPMI 2650 conditioned medium (CM) to stimulate growth in soft agar of RPMI 2650 cells plated at low density. The autocrine activity in crude CM can be fractionated by ultrafiltration into a lower molecular weight (MW) fraction (R1-30), which concentrates molecules in the 1000-30,000 Da range; and a higher MW fraction (R30) with molecules greater than 30,000 Da in a more concentrated form. R1-30 is labile to acid, base, and heat treatment, whereas R30 is stable to (and sometimes activated by) these treatments. Boiling of R30, however, renders it labile to acid, base, and trypsin treatments. CM can be separated into a weakly heparin-binding fraction (with stability properties similar, but not identical, to R1-30), and a non-heparin binding fraction (with stability properties similar to R30). RPMI 2650 cells secrete transforming growth factor (TGF)alpha- and TGF beta-like molecules, but the R1-30 fraction can be distinguished from these TGFs, and from most other known growth factors, by its unusual combination of acid lability and weak affinity for heparin. Since the R30/non-heparin binding fraction is rendered labile by boiling or acid treatment, it may represent a bound or conformationally stable form of a growth factor.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 2","pages":"153-60"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02630947","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18685806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2