In vitro cellular & developmental biology : journal of the Tissue Culture Association最新文献_第4页

Mitogens and hepatocyte growth control in vivo and in vitro 体内和体外有丝分裂原和肝细胞生长控制

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 2007-01-01 DOI: 10.1007/BF02623700

K. S. Koch, X. P. Lu, D. Brenner, G. Fey, A. Martinez-Conde, H. Leffert

引用次数: 1

Culturing of primary hepatocytes as entrapped aggregates in a packed bed bioreactor: a potential bioartificial liver. 原代肝细胞包裹聚集体在填充床生物反应器中的培养:一种潜在的生物人工肝。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634192

A P Li, G Barker, D Beck, S Colburn, R Monsell, C Pellegrin

{"title":"Culturing of primary hepatocytes as entrapped aggregates in a packed bed bioreactor: a potential bioartificial liver.","authors":"A P Li, G Barker, D Beck, S Colburn, R Monsell, C Pellegrin","doi":"10.1007/BF02634192","DOIUrl":"https://doi.org/10.1007/BF02634192","url":null,"abstract":"Conventional culture systems for hepatocytes generally involve cells cultured as flat, monolayer cells, with limited cell-cell contact, in a static pool of medium, unlike the liver in vivo where the parenchymal cells are cuboidal, with extensive cell-cell contact, and are continuously perfused with blood. We report here a novel bioreactor system for the culturing of primary hepatocytes with cuboidal cell shape, extensive cell-cell contact, and perfusing medium. The hepatocytes were inoculated into the bioreactor and allowed to recirculate at a rate optimal for them to collide and form aggregates. These newly-formed aggregates were subsequently entrapped in a packed bed of glass beads. The bioreactor was perfused with oxygenated nutrient medium, with controlled oxygen tension, pH, and medium perfusion rate. The hepatocytes were viable for up to the longest time point studied of 15 days in culture based on urea synthesis, albumin synthesis and cell morphology. Light microscopy studies of hepatocytes cultured for 15 days in the bioreactor showed interconnecting three-dimensional structures resembling the hepatic cell plate in the liver organ. Electron microscopy studies on the same cells revealed ultrastructure similar to the hepatocytes in vivo, including the presence of plentiful mitochondria, rough and smooth endoplasmic reticulum, glycogen granules, peroxisomes, and desmosomes. We believe that our hepatocyte bioreactor is a major improvement over conventional culture systems, with important industrial applications including toxicology, drug metabolism, and protein/peptide synthesis. The hepatocyte bioreactor concept may also be used as the basis for the development of a bioartificial liver to provide extracorporeal hepatic support to patients with hepatic failure.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 3 Pt 1","pages":"249-54"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634192","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19446535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Cellular models for multiple drug resistance in cancer. 癌症多重耐药的细胞模型。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634176

M Clynes

引用次数: 4

Stability of a murine hybridoma is dependent on the clonal line and culture media. 小鼠杂交瘤的稳定性依赖于无性系和培养基。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634184

N Kessler, S Bertrand, M Aymard

引用次数: 12

Epidermal growth factor and insulin-like growth factor-1 preserve cell viability in the absence of protein synthesis. 表皮生长因子和胰岛素样生长因子-1在缺乏蛋白质合成的情况下维持细胞活力。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634189

A Geier, R Hemi, M Haimson, R Beery

{"title":"Epidermal growth factor and insulin-like growth factor-1 preserve cell viability in the absence of protein synthesis.","authors":"A Geier, R Hemi, M Haimson, R Beery","doi":"10.1007/BF02634189","DOIUrl":"https://doi.org/10.1007/BF02634189","url":null,"abstract":"Prolonged exposure of cells to the potent protein synthesis inhibitor cycloheximide (CHX) terminates in cell death. In the present study we investigated the effect of epidermal growth factor (EGF), insulin-like growth factor-1 (IGF-1), and insulin on cell death induced by CHX in the human cancerous cell lines MDA-231 and MCF-7 (breast), KB (oral epidermoid), HEP-2 (larynx epidermoid), and SW-480 (colon), and correlated this effect to the inhibition rate of protein synthesis. Cell death was evaluated by measuring either dead cells by trypan blue dye exclusion test or by the release of lactic dehydrogenase into the culture medium. CHX was shown to induce cell death in a concentration (1 to 60 micrograms/ml) and time (24 to 72 h)-dependent manner in each of the five cell lines. EGF at physiologic concentrations (2 to 40 ng/ml) reduced cell death close to control level (without CHX) in the cell lines HEP-2, KB, MDA-231, and SW-480, but had almost no effect on cell death in the MCF-7 cells. IGF-1 at physiologic concentrations (2 to 40 ng/ml) reduced cell death nearly to control level in the MCF-7 cells, but had only a partial effect in the other four cell lines. Insulin at supraphysiologic concentration (10,000 ng/ml) mimicked the effect of IGF-1 in each of the cell lines. CHX at concentrations that induced about 60% cell death, inhibited about 90% of protein synthesis as measured by [3H]leucine incorporation. Protein synthesis remained inhibited although cell viability was preserved by EGF or IGF-1. These results indicated that the mechanism by which EGF or IGF-1 preserve cell viability does not require new protein synthesis and may be mediated via a posttranslational modification effect.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 3 Pt 1","pages":"231-4"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19446532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Measurement of strain in cultured bone and fetal muscle and lung cells. 培养骨、胎肌和肺细胞应变的测定。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634178

J E Anderson, R S Carvalho, E Yen, J E Scott

引用次数: 9

Auto and transactivation of FGF expression: potential mechanism for regulation of myogenic differentiation. FGF表达的自动和反激活:调节肌源性分化的潜在机制。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634188

J C Fox, J L Swain

{"title":"Auto and transactivation of FGF expression: potential mechanism for regulation of myogenic differentiation.","authors":"J C Fox, J L Swain","doi":"10.1007/BF02634188","DOIUrl":"https://doi.org/10.1007/BF02634188","url":null,"abstract":"Fibroblast growth factors (FGFs) are potent inhibitors of myogenic differentiation. The recent observation that the endogenous expression of acidic and basic FGF by myogenic cells decreases coordinately with differentiation suggests a regulatory role for these growth factors in myogenesis. Inasmuch as other proteins known to influence myogenesis (e.g., MyoD1 and myogenin) activate their own expression as well as the expression of other members of their family, we hypothesized that the FGFs might be capable of similar autoregulation. We examined the effect of exogenously supplied FGF on the abundance of the mRNAs encoding acidic and basic FGF in Sol 8 myoblasts, and demonstrate that either acidic or basic FGF stimulate, through paracrine mechanisms, the accumulation of the mRNAs encoding both of these FGFs. Thus FGFs can auto- and transregulate their own expression in a manner analogous to that observed for the myogenic determination proteins. In addition, similar to that previously observed for MyoD1, both acidic and basic FGF suppress myogenin expression in myoblasts. These results suggest two mechanisms whereby endogenously produced FGFs participate in the maintenance of the undifferentiated state of myogenic cells. These data provide support for paracrine, and suggest potential autocrine, roles for FGFs in the regulation of myogenic differentiation.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 3 Pt 1","pages":"228-30"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634188","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19370947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Nickel-induced increases in gap junctional communication in the uterine cell line SK-UT-1. 镍诱导的子宫细胞系SK-UT-1缝隙连接通讯的增加。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634186

M S Marty, R Loch-Caruso

引用次数: 1

Absence of trypsinogen autoactivation and immunolocalization of pancreatic secretory trypsin inhibitor in acinar cells in vitro. 胰蛋白酶原自身激活缺失及胰腺分泌型胰蛋白酶抑制剂在体外腺泡细胞中的免疫定位。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634187

A E Arias, T Böldicke, M Bendayan

{"title":"Absence of trypsinogen autoactivation and immunolocalization of pancreatic secretory trypsin inhibitor in acinar cells in vitro.","authors":"A E Arias, T Böldicke, M Bendayan","doi":"10.1007/BF02634187","DOIUrl":"https://doi.org/10.1007/BF02634187","url":null,"abstract":"To establish the significance of the addition of trypsin inhibitors to pancreatic acinar cells maintained in vitro, cells were cultured in the presence or absence of soybean trypsin inhibitor. Both cultures exhibited similar growth pattern, ultrastructural appearance, as well as secretory properties. Moreover, there was no evidence of trypsinogen activation in the culture medium. Using the immunocytochemical approach, pancreatic secretory trypsin inhibitor antigenic sites were revealed with specific polyclonal and monoclonal antibodies. The results obtained demonstrated that this trypsin inhibitor is in fact a typical pancreatic secretory protein being processed through the endoplasmic reticulum-Golgi-granule secretory pathway of the acinar cells in rat and human tissues. While the polyclonal antibody yield labelings of increasing intensities along the secretory pathway, the monoclonal one probably due to the molecular nature of its specific antigenic determinant, gave higher labelings in the endoplasmic reticulum. In conclusion the present study has shown that pancreatic acinar cells secrete a specific pancreatic trypsin inhibitor which most probably is involved in the mechanism to prevent trypsinogen activation.","PeriodicalId":77173,"journal":{"name":"In vitro cellular & developmental biology : journal of the Tissue Culture Association","volume":"29A 3 Pt 1","pages":"221-7"},"PeriodicalIF":0.0,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02634187","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19446533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Aphidicolin-resistant human cell lines resistant to UV and bleomycin. 抗紫外线和博来霉素的抗阿菲霉素人细胞系。

In vitro cellular & developmental biology : journal of the Tissue Culture Association Pub Date : 1993-03-01 DOI: 10.1007/BF02634182

K Kamino, K Fukuchi, K Tanaka, T Miki, J Nakura, Y Takemoto, Y Kumahara, T Ogihara

引用次数: 0