American Journal of Botany最新文献

{"title":"The non-flowering plants of a near-polar forest in East Gondwana, Tasmania, Australia, during the Early Eocene Climatic Optimum","authors":"Miriam A. Slodownik","doi":"10.1002/ajb2.16398","DOIUrl":"10.1002/ajb2.16398","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>The Cenozoic Macquarie Harbour Formation (MHF) hosts one of the oldest and southernmost post-Cretaceous fossil plant assemblages in Australia. Coinciding with the Early Eocene Climatic Optimum (EECO) and predating the breakup of Australia from Antarctica, it offers critical data to study the diversity and extent of the Austral Polar Forest Biome, and the floristic divergence between Australasia and South America resulting from the Gondwana breakup.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The micromorphology and macromorphology of new fossil plant compressions from the MHF were described and systematically analyzed. Previously published non-flowering plant records were reviewed and revised. Macrofossil abundance data were provided. The flora was compared with other early Paleogene assemblages from across the Southern Hemisphere.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Twelve species of non-flowering plants were identified from the macrofossil record. Conifers include Araucariaceae (<i>Araucaria macrophylla</i>, <i>A. readiae</i>, <i>A. timkarikensis</i> sp. nov., and <i>Araucarioides linearis</i>), Podocarpaceae (<i>Acmopyle glabra</i>, <i>Dacrycarpus mucronatus</i>, <i>Podocarpus paralungatikensis</i> sp. nov., and <i>Retrophyllum</i> sp.), and Cupressaceae (<i>Libocedrus microformis</i>). <i>Dacrycarpus linifolius</i> was designated a junior synonym of <i>D. mucronatus</i>. Further components include a cycad (<i>Bowenia johnsonii</i>, Zamiaceae), a pteridosperm (<i>Komlopteris cenozoicus</i>, Umkomasiaceae), and a fern (<i>Lygodium dinmorphyllum</i>, Schizaeaceae).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The fossil assemblage represents a mixed near-polar forest with a high diversity of conifers. The morphology and preservation of several species indicate adaptations to life at high latitudes. The coexistence of large- and small-leaved conifers implies complex, possibly open forest structures. Comparisons with contemporaneous assemblages from Argentina support a circumpolar biome during the EECO, reaching from southern Australia across Antarctica to southern South America.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ajb2.16398","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142078894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tank formation transforms nitrogen metabolism of an epiphytic bromeliad and its phyllosphere bacteria.","authors":"Jade Stryker, Elizabeth White, Erika Díaz-Almeyda, Brian Sidoti, Brad Oberle","doi":"10.1002/ajb2.16396","DOIUrl":"https://doi.org/10.1002/ajb2.16396","url":null,"abstract":"<p><strong>Premise: </strong>Up to half of tropical forest plant species grow on other plants. Lacking access to soils, vascular epiphytes have unique adaptations for mineral nutrition. Among the most distinctive is the tank growth form of certain large bromeliads, which absorb nutrients that are cycled by complex microbial communities in water trapped among their overlapping leaf bases. However, tanks form only after years of growth by juvenile plants, which must acquire nutrients differently. Understanding how nutrient dynamics change during tank bromeliad development can provide key insights into the role of microorganisms in the maintenance of tropical forest biodiversity.</p><p><strong>Methods: </strong>We evaluated variations in plant morphology, growth, foliar nitrogen physiology, and phyllosphere bacterial communities along a size gradient spanning the transition to tank formation in the threatened species Tillandsia utriculata.</p><p><strong>Results: </strong>Sequential morphological and growth phases coincided with the transition to tank formation when the longest leaf on plants was between 14 and 19 cm. Before this point, foliar ammonium concentrations were very high, but after, leaf segments absorbed significantly more nitrate. Leaf-surface bacterial communities tracked ontogenetic changes in plant morphology and nitrogen metabolism, with less-diverse communities in tankless plants distinguished by a high proportion of taxa implicated in ureolysis, nitrogen fixation, and methanotrophy, whereas nitrate reduction characterized communities on individuals that could form a tank.</p><p><strong>Conclusions: </strong>Coupled changes in plant morphology, physiology, and microbiome function facilitate the transition between alternative nutritional modes in tank bromeliads. Comparing bromeliads across life stages and habitats may illuminate how nitrogen-use varies across scales.</p>","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Doubling down on polyploid discoveries: Global advances in genomics and ecological impacts of polyploidy","authors":"Michael S. Barker,&nbsp;Yuannian Jiao,&nbsp;Kelsey L. Glennon","doi":"10.1002/ajb2.16395","DOIUrl":"10.1002/ajb2.16395","url":null,"abstract":"<p>All flowering plants are now recognized as diploidized paleopolyploids (Jiao et al., 2011; One Thousand Plant Transcriptomes Initiative, 2019), and polyploid species comprise approximately 30% of contemporary plant species (Wood et al., 2009; Barker et al., 2016a). A major implication of these discoveries is that, to appreciate the evolution of plant diversity, we need to understand the fundamental biology of polyploids and diploidization. This need is broadly recognized by our community as there is a continued, growing interest in polyploidy as a research topic. Over the past 25 years, the sequencing and analysis of plant genomes has revolutionized our understanding of the importance of polyploid speciation to the evolution of land plants.</p>","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ajb2.16395","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inbreeding in Chinese fir: Insights into the adaptive growth traits of selfed progeny from mRNA, miRNA, and copy number variation","authors":"Houyin Deng,&nbsp;Rong Huang,&nbsp;Ruping Wei,&nbsp;Runhui Wang,&nbsp;Shu Yan,&nbsp;Yousry A. El-Kassaby,&nbsp;Yuhan Sun,&nbsp;Yun Li,&nbsp;Huiquan Zheng","doi":"10.1002/ajb2.16393","DOIUrl":"10.1002/ajb2.16393","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>The impact of inbreeding on biological processes is well documented in individuals with severe inbreeding depression. However, the biological processes influencing the adaptive growth of normal selfed individuals are unknown. Here, we aimed to investigate how inbreeding affects gene expression for adaptive growth of normal selfed seedlings from a self-fertilizing parent in Chinese fir (<i>Cunninghamia lanceolata</i>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Using RNA-seq data from above- and underground tissues of abnormal and normal selfed seedlings, we analyzed GO biological processes network. We also sequenced small RNAs in the aboveground tissues and measured the copy number variations (CNV) of the hub genes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Phenotypic fitness analysis revealed that the normal seedlings were better adapted than their abnormal counterparts. Upregulated differentially expressed genes (DEGs) were associated with development processes, and downregulated DEGs were mainly enriched in fundamental metabolism and stress response. Results of mRNA-miRNA parallel sequencing revealed that upregulated target genes were predominantly associated with development, highlighting their crucial role in phosphorylation in signal transduction networks. We also discovered a moderate correlation (0.1328 &lt; <i>R</i>² &lt; 0.6257) between CNV and gene expression levels for three hub genes (<i>TMKL1</i>, <i>GT2</i>, and <i>RHY1A</i>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We uncovered the key biological processes underpinning the growth of normal selfed seedlings and established the relationship between CNV and the expression levels of hub genes in selfed seedlings. Understanding the candidate genes involved in the growth of selfed seedlings will help us comprehend the genetic mechanisms behind inbreeding depression in the evolutionary biology of plants.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"All tangled up: Unraveling phylogenetics and reticulate evolution in the vining ferns, Lygodium (Schizaeales)","authors":"Jessie A. Pelosi,&nbsp;Bethany A. Zumwalde,&nbsp;Weston L. Testo,&nbsp;Emily H. Kim,&nbsp;J. Gordon Burleigh,&nbsp;Emily B. Sessa","doi":"10.1002/ajb2.16389","DOIUrl":"10.1002/ajb2.16389","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>Reticulate evolution, often accompanied by polyploidy, is prevalent in plants, and particularly in the ferns. Resolving the resulting non-bifurcating histories remains a major challenge for plant phylogenetics. Here, we present a phylogenomic investigation into the complex evolutionary history of the vining ferns, <i>Lygodium</i> (Lygodiaceae, Schizaeales).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Using a targeted enrichment approach with the <i>GoFlag 408</i> flagellate land plant probe set, we generated large nuclear and plastid sequence datasets for nearly all taxa in the genus and constructed the most comprehensive phylogeny of the family to date using concatenated maximum likelihood and coalescence approaches. We integrated this phylogeny with cytological and spore data to explore karyotype evolution and generate hypotheses about the origins of putative polyploids and hybrids.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Our data and analyses support the origins of several putative allopolyploids (e.g., <i>L. cubense, L. heterodoxum</i>) and hybrids (e.g., <i>L.</i> ×<i>fayae</i>) and also highlight the potential prevalence of autopolyploidy in this clade (e.g., <i>L. articulatum, L. flexuosum</i>, and <i>L. longifolium</i>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our robust phylogenetic framework provides valuable insights into dynamic reticulate evolution in this clade and demonstrates the utility of target-capture data for resolving these complex relationships.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ajb2.16389","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Living collections: Biodiversity cultivated at public gardens has the power to connect ecological questions and evolutionary context","authors":"Jean H. Burns,&nbsp;Katharine L. Stuble,&nbsp;Juliana S. Medeiros","doi":"10.1002/ajb2.16394","DOIUrl":"10.1002/ajb2.16394","url":null,"abstract":"&lt;p&gt;Combining ecological questions with evolutionary context generates novel insight into both ecology and evolution. However, our ability to draw broad inferences can be limited by the taxonomic diversity present within and across species at a site. Public gardens (including botanical gardens and arboreta) may focus solely on aesthetics in developing their gardens, but some public gardens include scientific inquiry and conservation at the core of their missions (Hohn, &lt;span&gt;2022&lt;/span&gt;). These scientifically oriented public gardens follow community standards of excellence (Hohn, &lt;span&gt;2022&lt;/span&gt;) to provide unique access to curated plant collections specifically designed to gather high levels of biodiversity, both among and within species, at a single geographic location. These research-grade collections include long-lived species cared for over many decades. Such public gardens have long histories of conducting and supporting research harnessing the power inherent in these diverse collections, including explorations of systematics, ecophysiology, and ecology. By bringing together species, as well as individuals within species, from across broad spatial ranges into a single site, these collections offer living repositories of diversity ripe for scientific exploration as de facto common gardens (Dosmann, &lt;span&gt;2006&lt;/span&gt;; Dosmann and Groover, &lt;span&gt;2012&lt;/span&gt;; Primack et al., &lt;span&gt;2021&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;The biodiversity curated by public gardens can offer a unique context for addressing questions at the intersection of ecology and evolution, such as &lt;i&gt;how does phylogenetic history shape plant trait evolution?&lt;/i&gt; For example, Mason et al. (&lt;span&gt;2020&lt;/span&gt;) explored seasonal trait shifts across 25 species of &lt;i&gt;Cornus&lt;/i&gt; at the Arnold Arboretum (Boston, Massachusetts, USA) to ask whether there are tradeoffs among ecophysiological traits and how those traits correlate with home environment. They measured traits such as leaf chlorophyll content and leaf water content. By measuring plant traits across many species, they answered questions about ecophysiological trait evolution within a comparative phylogenetic framework. By doing so in a common garden, they controlled for much of the environmental variation that would otherwise confound a study across so many species, that occur in different habitats and locations in their native ranges. Their new analytical approaches simultaneously incorporated phylogenetic methods and within-species variation over time (Mason et al., &lt;span&gt;2020&lt;/span&gt;). With this comparison, they demonstrated that traditional phylogenetic comparative approaches, which analyze a single trait mean per species, might come to erroneous conclusions about trait–trait correlations. For example, leaf nitrogen mostly declines through the growing season in &lt;i&gt;Cornus&lt;/i&gt;, leading to changes in sign of correlations across the season (Mason et al., &lt;span&gt;2020&lt;/span&gt;).&lt;/p&gt;&lt;p&gt;Plant–soil interactions are another growing area of research that ","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ajb2.16394","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative morphology at a crossroads","authors":"Julius Jeiter,&nbsp;Erik Smets","doi":"10.1002/ajb2.16392","DOIUrl":"10.1002/ajb2.16392","url":null,"abstract":"<p>Morphology has been the fundamental and most important source of information in biology. We strongly believe that in the current molecular era of biology, comparative morphology still has an important role to play in understanding life on Earth and ecosystem functioning, bridging the knowledge gap between evolution, systematics, and ecology.</p>","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ajb2.16392","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperaccumulation of nickel but not selenium drives floral microbiome differentiation: A study with six species of Brassicaceae","authors":"Nevin P. Cullen,&nbsp;Tia-Lynn Ashman","doi":"10.1002/ajb2.16382","DOIUrl":"10.1002/ajb2.16382","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>Intraspecific variation in flower microbiome composition can mediate pollination and reproduction, and so understanding the community assembly processes driving this variation is critical. Yet the relative importance of trait-based host filtering and dispersal in shaping among-species variation in floral microbiomes remains unknown.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Within two clades of Brassicaceae, we compared diversity and composition of floral microbiomes in natural populations of focal nickel and selenium hyperaccumulator species and two of their non-accumulating relatives. We assessed the relative strengths of floral elemental composition, plant phylogenetic distance (host filtering), and geography (dispersal) in driving floral microbiome composition.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Species in the nickel hyperaccumulator clade had strongly divergent floral microbiomes, the most of that variation driven by floral elemental composition, followed by geographic distance between plant populations and, lastly, phylogenetic distance. Conversely, within the selenium hyperaccumulator clade, floral microbiome divergence was much lower among the species and elemental composition, geography, and plant phylogeny were far weaker determinants of microbiome variation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Our results show that the strength of elemental hyperaccumulation's effect on floral microbiomes differs substantially among plant clades, possibly due to variation in elements as selective filters or in long-distance dispersal probability in different habitats.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ajb2.16382","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fisher's automatic advantage of self-fertilization does not apply in cleistogamous species","authors":"Pierre-Olivier Cheptou","doi":"10.1002/ajb2.16390","DOIUrl":"10.1002/ajb2.16390","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>In hermaphroditic plants, the evolution of self-fertilization is driven by two major forces; the cost of outcrossing or Fisher's automatic advantage of selfing and inbreeding depression. Seminal theoretical works have established that an inbreeding depression threshold of 0.5 governs the evolution. Below that threshold, selfing evolves, above that, outcrossing evolves. Does this threshold apply to cleistogamous plants?</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>I developed a model using a Lloydian approach to analyze the evolution of cleistogamy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>I showed that the inbreeding depression threshold does not apply in cleistogamous species, and that because cleistogamous (closed) flowers do not export pollen, Fisher's advantage of selfing is totally cancelled.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>In line with model predictions, I discuss the fact that cleistogamous species often exhibit low inbreeding depression in empirical studies.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of deep hybridization in fern speciation: Examples from the Thelypteridaceae","authors":"Yu-Hsin Tseng,&nbsp;Li-Yaung Kuo,&nbsp;Israel Borokini,&nbsp;Susan Fawcett","doi":"10.1002/ajb2.16388","DOIUrl":"10.1002/ajb2.16388","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Premise</h3>\u0000 \u0000 <p>Hybridization is recognized as an important mechanism in fern speciation, with many allopolyploids known among congeners, as well as evidence of ancient genome duplications. Several contemporary instances of deep (intergeneric) hybridization have been noted, invariably resulting in sterile progeny. We chose the christelloid lineage of the family Thelypteridaceae, recognized for its high frequency of both intra- and intergeneric hybrids, to investigate recent hybrid speciation between deeply diverged lineages. We also seek to understand the ecological and evolutionary outcomes of resulting lineages across the landscape.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>By phasing captured reads within a phylogenomic data set of GoFlag 408 nuclear loci using HybPhaser, we investigated candidate hybrids to identify parental lineages. We estimated divergence ages by inferring a dated phylogeny using fossil calibrations with treePL. We investigated ecological niche conservatism between one confirmed intergeneric allotetraploid and its diploid progenitors using the centroid, overlap, unfilling, and expansion (COUE) framework.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We provide evidence for at least six instances of intergeneric hybrid speciation within the christelloid clade and estimate up to 45 million years of divergence between progenitors. The niche quantification analysis showed moderate niche overlap between an allopolyploid species and its progenitors, with significant divergence from the niche of one progenitor and conservatism to the other.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The examples provided here highlight the overlooked role that allopolyploidization following intergeneric hybridization may play in fern diversification and range and niche expansions. Applying this approach to other fern taxa may reveal a similar pattern of deep hybridization resulting in highly successful novel lineages.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7691,"journal":{"name":"American Journal of Botany","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ajb2.16388","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}