Journal de biologie buccale最新文献_第8页

A rat incisor dentin matrix protein can induce neonatal rat muscle fibroblasts, in culture, to express phenotypic products of chondroblastic cells. 大鼠门牙本质基质蛋白可以诱导培养的新生大鼠肌肉成纤维细胞表达成软骨细胞的表型产物。

Journal de biologie buccale Pub Date : 1991-03-01

S Amar, B Sires, A Veis

{"title":"A rat incisor dentin matrix protein can induce neonatal rat muscle fibroblasts, in culture, to express phenotypic products of chondroblastic cells.","authors":"S Amar, B Sires, A Veis","doi":"","DOIUrl":"","url":null,"abstract":"Demineralized dentin matrix induces the ectopic formation of bone, in vivo, when implanted subcutaneously or in muscle pouches. In these situations the bone induction follows a chondrogenic pathway. As part of the strategy for the assay and isolation of the factors responsible for initiating induction, we have developed a cell culture system in which the addition of soluble factors extracted from the dentin matrix appears to initiate chondrogenesis. Indicators of chondrogenesis, relative to control cultures, were taken as an increase of 35S-sulfate incorporation into proteoglycan (PG), an altered size of the PG, production of type II collagen, and changes in cell morphology and matrix histochemistry. Our studies have taken two directions: the use of the cell culture system under standard conditions to select fractions inducing one or more of the above indica-tors; and, the purification and characterization of the in vitro chondrogenesis inducing factor(s). Here we report the identification of a peptide fraction which acts in culture to satisfy each of the above indicators of chondrogenesis. An EDTA extract of rat incisor dentin was fractionated by CaCl2 precipitation, Sephacryl S-100 chromatography, and reverse phase HPLC. A single peptide fraction from the HPLC, evidenced by the existence of a single spot on 2-D Gel Electrophoresis, was found to be a potent enhancer of 35S-sulfate incorporation during the standard assay, with maximal activity in the 1-10 ng/ml range. Further detailed studies showed that the heightened incorporation occurred without any increase in cell number. The neonatal rat muscle explant fibroblasts exposed to this fraction for 7 days in monolayer culture formed dense cell nodules which stained intensely with Alcian blue relative to controls.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 1","pages":"55-60"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13025280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Oral bacterial flora and its pathogenic potential]. [口腔菌群及其致病潜力]。

Journal de biologie buccale Pub Date : 1991-03-01

I Madinier

引用次数: 0

The effect of an antimicrobial releasing varnish on root demineralisation in situ. The influence of the demineralisation period. 抗菌释放清漆对根原位脱矿的影响。脱矿期的影响。

Journal de biologie buccale Pub Date : 1991-03-01

E D Huizinga, J Arends

{"title":"The effect of an antimicrobial releasing varnish on root demineralisation in situ. The influence of the demineralisation period.","authors":"E D Huizinga, J Arends","doi":"","DOIUrl":"","url":null,"abstract":"Caries in exposed root surfaces is becoming a significant problem in dentistry. Varnish applications have interesting preventive possibilities against root caries. The effect of such a varnish, containing two antimicrobials, was studied on the demineralisation of roots with a twofold aim. Firstly, the efficacy of the varnish was investigated in situ as a function of the demineralisation period. Ten participants wore sound intact roots treated with the varnish in an intra oral appliance for 2, 4 and 6 weeks. Secondly, the effect of the varnish on root demineralisation after removal of the varnish was measured. The results showed that the reduction, in demineralization of about 80% after 2 weeks, decreased in magnitude with the length of the in situ demineralisation period. After 4 weeks there was no longer statistically significant difference compared with untreated roots. The acid attack by plaque was, however, substantial in this model; after 6 weeks in situ untreated roots were so strongly demineralised that microradiography was no longer possible anymore. In the second experiment in which the varnish was removed 2 days after application there was a trend toward a reduction of about 30% in root demineralisation after 2 weeks. This was not statistically significant when compared with no application.","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 1","pages":"29-33"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13025556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rates of protein turnover at specific sites of the rat incisor periodontal ligament. 大鼠门牙牙周韧带特定部位的蛋白质周转率。

Journal de biologie buccale Pub Date : 1991-03-01

J Kirkham, C Robinson, R C Shore

引用次数: 0

An assessment of the control of matrix turnover by a quantitative ultrastructural analysis of fibroblasts of the periodontal ligament in rats. 大鼠牙周韧带成纤维细胞超微结构定量分析对基质转换的控制作用。

Journal de biologie buccale Pub Date : 1991-03-01

R C Shore, J Kirkham, C Robinson, B J Moxham, B K Berkovitz

引用次数: 0

Calcium-binding proteins: an overview. 钙结合蛋白综述。

Journal de biologie buccale Pub Date : 1991-03-01

S Weinman

{"title":"Calcium-binding proteins: an overview.","authors":"S Weinman","doi":"","DOIUrl":"","url":null,"abstract":"In order to understand the mechanism of the various responses evoked by calcium in the cell, the identification and characterization of a number of calcium receptors were undertaken within the past two decades. Advances in amino acid sequence and protein three-dimensional structure led to the description of two families of calcium-binding proteins, the EF-hand homolog family and the annexin family. The EF-hand motif consists of two alpha helices, \"E\" and \"F\", joined by a Ca(2+)-binding loop. EF-hands have been identified in numerous Ca(2+)-binding proteins by similarity of amino acid sequence and confirmed in some crystal structures. Functional EF-hands seem always to occur in pairs. To date, the EF-hand homolog family contains more than 160 different Ca(2+)-modulated proteins which have a broad range of functions. Among them, are the calmodulin, the troponin C, the myosin regulatory light chain, the parvalbumin, the S-100 proteins and the calbindins 9- and 28 kDa. The most striking feature of the EF-hand family is the ability to modulate the activity of a number of enzymes. Several groups have identified proteins from various tissues that show calcium-dependent binding to membranes. These proteins, termed annexins have a molecular weight of 35- or 67 kDa. The amino acid sequences of the members of the annexin family show that each protein contains conserved internal repeats of about 70 amino acids each. The 35 kDa annexins contain four repeats, which show a high degree of homology with each other and with the repeat sequences of the other proteins. These repeats correspond to structural domains with a similar fold.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 1","pages":"90-8"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13025171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mineralization disturbances of the developing rat molar induced by mono- and bisphosphonates. 单膦酸盐和双膦酸盐对发育中的大鼠磨牙矿化的影响。

Journal de biologie buccale Pub Date : 1991-03-01

N Fouda, M Caracatsanis, I A Kut, L Hammarström

{"title":"Mineralization disturbances of the developing rat molar induced by mono- and bisphosphonates.","authors":"N Fouda, M Caracatsanis, I A Kut, L Hammarström","doi":"","DOIUrl":"","url":null,"abstract":"Morphological changes have been reported previously in the developing dental tissues of the rat molar following administration of mono- or bisphosphonates. However, few studies have been published on the relationship between these and the possible mineralization disturbances. Two monophosphonates, phosphonoformic and phosphonoacetic acids, and two bisphosphonates, dichloromethylene bisphosphonate (Cl2MBP) and 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) in a dosage of 10-30 mg P/kg b.w. were injected in 5 day old rats and the induced changes analyzed by microradiographic and histologic examination. A single injection of mono- or bisphosphonates resulted in apparently identical disturbances of the developing enamel associated with subameloblastic cysts. Three different types of cysts were distinguished. In type 1 cysts, enamel hypoplasia near the cusp tip with an outer highly mineralized zone associated with calcified deposits in the cystic lining and inner hypomineralized enamel were discerned. The type 2 cysts were characterized by the formation of a thick enamel layer in the cervical area and deep in the fissures due to the development of internal enamel defects. The type 3 cysts were seen in the cervical area of the mesial or distal sides of the tooth without any remarkable changes in the underlying enamel matrix. Both Cl2MBP and HEBP induced two demarcation lines in the dentin layer. Additionally, HEBP resulted in the formation of niche-like defects of unmineralized dentin which healed by mineral deposition from the periphery. From these results, it can be concluded that mono- and bisphosphonates have similar effects on enamel mineralization and the type of response depends on the stage of enamel matrix secretion while bisphosphonates, in addition, interfere with the dentin mineralization.","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 1","pages":"106-15"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12993360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sialoproteins of bone and dentin. 骨和牙本质的唾液蛋白。

Journal de biologie buccale Pub Date : 1991-03-01

W T Butler

引用次数: 0

Embryology and secretory activity of labial salivary glands. 胚胎学与唇唾液腺分泌活性。

Journal de biologie buccale Pub Date : 1991-03-01

M Muller, J R Jasmin, R A Monteil, R Loubiere

引用次数: 0

The cellular and extracellular distribution of osteocalcin and dentin phosphoprotein in teeth of vitamin D-deficient rats. 维生素d缺乏大鼠牙内骨钙素和牙本质磷蛋白的细胞外分布。

Journal de biologie buccale Pub Date : 1991-03-01

A Berdal, I Gorter de Vries, D Hotton, P Cuisinier-Gleizes, H Mathieu

{"title":"The cellular and extracellular distribution of osteocalcin and dentin phosphoprotein in teeth of vitamin D-deficient rats.","authors":"A Berdal, I Gorter de Vries, D Hotton, P Cuisinier-Gleizes, H Mathieu","doi":"","DOIUrl":"","url":null,"abstract":"Experimental and clinical data indicate that dentin mineralization is vitamin D-dependent. This calcium-regulating steroid controls protein synthesis, for instance that of osteocalcin in osteoblasts. This protein also elaborated by odontoblasts was used as a molecular marker for vitamin D action on odontoblasts. Since the most characteristic protein synthesized by odontoblasts is the dentin phosphoprotein which is thought to regulate hydroxyapatite growth, its cellular and extracellular distribution was also studied. Tooth formation in the molars and incisors of successive generations of vitamin D-deficient animals (-D) and in controls (+D) was compared by microadiography, toluidine blue histochemistry, and immunocytochemistry. In -D samples, the presence of dentin phosphoprotein in odontoblasts indicated that their differentiation occurred despite major morphological disturbances at the cusp tips. In contralateral teeth, osteocalcin was depleted in odontoblasts and dentin, suggesting an inhibition of protein synthesis induced by vitamin D-deficiency. In the extracellular matrix of +D animals, phosphoprotein distribution was associated with dentin, especially within actively forming calcospherites at the mineralization front. In contrast, in -D dentin, the mineralization defects corresponded to irregular absence of histochemically detectable phosphoprotein. This protein indeed appeared either absent or uniformly sparse in -D dentin by immunocytochemistry. These data suggest that vitamin D acts directly on odontogenic cells at various synthetic (osteocalcin) or secretory (phosphoprotein) levels indicating that odontoblasts are target-cells for vitamin D. Therefore, this hormone could contribute to the regulation of extracellular mineralization during dentinogenesis, via different mechanisms in the processing of matrix protein.","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 1","pages":"45-53"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13025279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0