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[6 dentin adhesive products subjected to controls in vitro]. [6种牙本质黏合剂产品的体外对照]。
Journal de biologie buccale Pub Date : 1990-12-01
M Neroni, C Regad, O Christen, S Thoenen, J Holz
{"title":"[6 dentin adhesive products subjected to controls in vitro].","authors":"M Neroni,&nbsp;C Regad,&nbsp;O Christen,&nbsp;S Thoenen,&nbsp;J Holz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this in vitro study was to assess of the biocompatibility of 6 adhesive dental products (Cavalite, Dual Cement, Fuji Type II, Gluma, Ketac Bond, Scotch Bond) using cell cultures of human pulps and a standardized method. It was thus possible to improve the scale of biological activity proposed by Regad et al., (1989). The specific behaviour of the cells in the contact and adjacent zones has been added to the assessment of the cellular reactions of the whole culture. These criteria allowed the clear differentiation of the toxicity and the biocompatibility of the products. The following estimations were made: Gluma and Fuji Type II \"toxic\", Cavalite \"acceptable\", Scotch Bond \"not significant\", Ketac bond and Dual Cement \"acceptable\" and \"not significant\" with a \"toxic\" tendency.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"271-85"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13123312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extracellular processing of enamel matrix proteins and the control of crystal growth. 釉质基质蛋白的胞外加工与晶体生长的控制。
Journal de biologie buccale Pub Date : 1990-12-01
C Robinson, R C Shore, J Kirkham, N J Stonehouse
{"title":"Extracellular processing of enamel matrix proteins and the control of crystal growth.","authors":"C Robinson,&nbsp;R C Shore,&nbsp;J Kirkham,&nbsp;N J Stonehouse","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The origin, expression and role of matrix modifying enzymes in dental enamel was investigated by zymography and growth of enamel crystals in vitro. Gelatinase activity of the neutral metalloprotease type was detected at similar molecular weights in enamel organ, enamel and dentine. The activity was present throughout all developmental stages in enamel organ but was dramatically reduced in the maturation stage of the enamel. Activity of the serine protease type directed against enamel matrix was also detected in enamel, particularly in the maturation stage. No such activity was detected in the enamel organ. Phosphatase activity at alkaline pH was demonstrated at similar molecular weights in both enamel and enamel organ. This activity was maximal in the maturation stage. Further experiments showed that both serine proteases and alkaline phosphatase were able to facilitate enamel crystal growth in vitro. Matrix modification via temporally and spatially restricted enzymes may be directly involved in the control of enamel crystal growth and hence in the determination of final tissue architecture.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"355-61"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13123311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histochemical localization of transforming growth factor-beta 1 in developing rat molars using antibodies to different epitopes. 转化生长因子- β 1在大鼠磨牙发育中的组织化学定位。
Journal de biologie buccale Pub Date : 1990-12-01
R N D'Souza, R P Happonen, K C Flanders, W T Butler
{"title":"Histochemical localization of transforming growth factor-beta 1 in developing rat molars using antibodies to different epitopes.","authors":"R N D'Souza,&nbsp;R P Happonen,&nbsp;K C Flanders,&nbsp;W T Butler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this study transforming growth factor-beta 1 (TGF-beta 1) has been immunolocalized in developing rat molars using two well characterized polyclonal antibodies, Anti-CC and Anti-LC, that recognize extracellular and intracellular TGF-beta 1, respectively. With immunohistochemical methods and the ABC-peroxidase system of detection, the growth factor was immunolocalized within the ectodermally derived enamel organ and the neural crest-derived dental papilla at the early and advanced bell stages of development. With Anti-CC, widespread and abundant extracellular TGF-beta 1 was found associated with the stellate reticulum and within central and apical regions of dental papilla mesenchyme. In contrast, Anti-LC localized TGF-beta 1 intensely within the cells of the outer dental epithelium. Moderate immunostaining for TGF-beta 1 with Anti-LC was also evident within the apical cytoplasm of inner dental epithelial cells and odontoblasts. These findings support the hypothesis that TGF-beta 1 may play a paracrine role in tooth development by regulating the epithelial-mesenchymal interactions that influence growth and cytodifferentiation events.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"299-306"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12874364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of testosterone on proteoglycans in the submandibular glands of the rat. 睾酮对大鼠颌下腺蛋白聚糖的影响。
Journal de biologie buccale Pub Date : 1990-12-01
J A Kofoed, O R Tumilasci, H M Curbelo, S M Fernandez Lemos, N H Arias, A B Houssay
{"title":"Effect of testosterone on proteoglycans in the submandibular glands of the rat.","authors":"J A Kofoed,&nbsp;O R Tumilasci,&nbsp;H M Curbelo,&nbsp;S M Fernandez Lemos,&nbsp;N H Arias,&nbsp;A B Houssay","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The proteoglycans in the submandibular salivary glands of castrated male Wistar rats were studied before and after the daily administration of testosterone propionate (TP) for one month. Castration decreased the weight of the glands and their uronic acid content. The administration of TP reversed these effects. Chromatographic separation of the uronic acid fractions was performed on cellulose microcolumns. The principal fractions were hyaluronic acid, heparan sulfate and dermatan sulfate. There were also changes in the physical properties of the proteoglycans. Castration decreased the range of distribution of molecular weight and the density, while the lateral chains of smaller length disappeared. TP administration to castrated rats reversed these effects.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"255-9"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13284106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Annexins I-VI in secretory ameloblasts and odontoblasts of rat incisor. 大鼠门牙分泌性成釉细胞和成牙细胞中膜联蛋白I-VI的表达。
Journal de biologie buccale Pub Date : 1990-12-01
M Goldberg, J Feinberg, D Rainteau, S Lecolle, M A Kaetzel, J R Dedman, S Weinman
{"title":"Annexins I-VI in secretory ameloblasts and odontoblasts of rat incisor.","authors":"M Goldberg,&nbsp;J Feinberg,&nbsp;D Rainteau,&nbsp;S Lecolle,&nbsp;M A Kaetzel,&nbsp;J R Dedman,&nbsp;S Weinman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immunoblot analyses and ultrastructural immunogold studies have been conducted on annexins in the secretory ameloblasts and odontoblasts of the rat incisor. Annexins I and II were seen in the soluble and particulate fractions of the enamel-related portion but not in the dentin-related portion. These proteins were visualized in the cytosol, near to the plasma membrane of Tomes' processes and in secretory vesicles in the ameloblasts. The forming enamel was also labeled. Annexins III, VI an V were detected in both the soluble and particulate fractions of the enamel-and dentin-related portions. Annexin IV was mainly localized in the proximal and distal areas of the secretory ameloblasts and virtually absent from in the supranuclear area. Annexin V was mainly detected in the cytosol of the cells and to a lesser extent near the plasma membrane. Annexin VI was mainly detected in the particulate fraction of enamel- and dentin-related portions. It was seen in the mitochondria and in the subplasmalemmal undercoat. All these proteins may play a role in exocytosis and endocytosis. They are implied in the regulation of cell calcium, but not in the transfer of calcium through the cells in the direction of the forming enamel and dentin, except annexins I and II since they are both present in the secretory vesicles and in the forming enamel.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"289-98"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13306111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The development of the periodontal ligament with special reference to collagen fibre ontogeny. 牙周韧带的发育，特别与胶原纤维的发生有关。
Journal de biologie buccale Pub Date : 1990-09-01
B K Berkovitz, B J Moxham
{"title":"The development of the periodontal ligament with special reference to collagen fibre ontogeny.","authors":"B K Berkovitz,&nbsp;B J Moxham","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The development of the periodontal ligament was investigated from serial sections of the mandibles of ferrets ranging in age from 42 days to 66 days post partum. Both succedaneous and non-succedaneous teeth were studied and similarities were seen in both types of tooth. No significant amounts of alveolar bone were observed being deposited beneath the erupting root apices and periodontal collagen fibres were seen attached to the walls of the sockets just prior to emergence of the crown into the oral cavity. Compared with the condition a few days after eruption, the collagen fibres on, and just prior to, emergence were thinner, had a more limited area of attachment, and were more randomly orientated. The results indicate that alveolar bone deposition and collagen traction do not generate the eruptive force and that there are species differences in these structural features during the eruptive process.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 3","pages":"227-36"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13405940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Solubility of human enamel mineral. 人牙釉质矿物的溶解度。
Journal de biologie buccale Pub Date : 1990-09-01
E C Moreno, T Aoba
{"title":"Solubility of human enamel mineral.","authors":"E C Moreno,&nbsp;T Aoba","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The solubility of human enamel was investigated in dilute phosphoric acid solutions (0.07 mMols/L to 1.54 mMols/L) under controlled partial pressures of CO2 (0.005% to 3.33% in mixtures with N2) at 25 degrees C. The enamel mineral was considered as carbonatoapatite containing acid phosphate having the stoichiometry [Ca]5-x-y[HPO4]v[PO4]3-x[CO3]w[OH]l-x-y. During equilibration, a second apatitic phase precipitated, as observed under the electronmicroscope. Solubility models are advanced consistent with the adapted stoichiometry and the precipitation of the second phase. The latter appears to have a carbonate content similar to that of the enamel mineral even when precipitated under the lowest partial pressure of CO2, suggesting that a molar content of about 0.3 of carbonate stabilizes the apatitic lattice. Based on the adopted stoichiometry of enamel mineral, Ca4.34 (HPO4).08 (CO3).29 (PO4)2.63 (OH).05, its solubility product constant was calculated as Ksp = 1.74 (+/- 0.081)x10(-6), expressed as the mean activity product.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 3","pages":"195-201"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13328436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Evaluation of the gingival status by quantification of the T6 (CD1a) and HLA-DR antigens]. [T6 (CD1a)和HLA-DR抗原定量评价牙龈状态]。
Journal de biologie buccale Pub Date : 1990-09-01
E Sagredo, A Pino, P Ibañez
{"title":"[Evaluation of the gingival status by quantification of the T6 (CD1a) and HLA-DR antigens].","authors":"E Sagredo,&nbsp;A Pino,&nbsp;P Ibañez","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The number/mm2 of sections of Langerhans cells (LC's) expressing antigens T6 and/or HLA-DR was determined in eight human gingival samples which consisting of normal gingivae, contraceptive induced hyperplastic, traumatic, inflammatory and diphenylhydantoin hyperplasic gingivae. All samples, except those from normal gingivae, showed inflammatory and/or hyperplasic histopathological patterns. In the immunostained samples, LC's appeared in variable numbers and were randomly distributed between the keratinocytes. All pathological samples, except the inflammatory gingiva showed HLA-DR+ keratinocytes. Wilcoxon and Mann-Whitney statistical tests suggested the existence of three subpopulations of LC's: T6+/DR+; T6-/DR+ and T6+/DR-. The subpopulation T6+/DR+ was predominant in conditions of normal immune response. The subpopulation T6-/DR+ suggested the presence of newly incorporated LC's from the lamina propia. T6+/DR- subpopulations could indicate the existence of a system to avoid overstimulation. It is concluded that quantitative evaluation of T6 and HLA-DR expression was representative of the gingival conditions and the disease activity.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 3","pages":"163-8"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12866234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of attachment proteins during cementogenesis. 胶凝形成过程中附着蛋白的表达。
Journal de biologie buccale Pub Date : 1990-09-01
M J Somerman, B Shroff, W S Agraves, G Morrison, A M Craig, D T Denhardt, R A Foster, J J Sauk
{"title":"Expression of attachment proteins during cementogenesis.","authors":"M J Somerman,&nbsp;B Shroff,&nbsp;W S Agraves,&nbsp;G Morrison,&nbsp;A M Craig,&nbsp;D T Denhardt,&nbsp;R A Foster,&nbsp;J J Sauk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>There is general agreement that during development the extracellular environment plays a critical role in controlling cell differentiation. Data generated from numerous studies support the possibility that cell attachment proteins and their corresponding cell receptors are possible candidates for this role. In particular, our studies are directed at identifying attachment proteins in mature cementum and establishing the function of these proteins during root formation. Fractionation of guanidine HCL/EDTA extracts of cementum revealed the presence of a bone-associated attachment, BSP, as well as fractions containing as of yet undetermined attachment proteins. Immunofluorescent examination of 1st molar tissues during root formation, obtained from 7 day-old mice neonates, for bone-associated attachment proteins indicated that osteopontin is expressed in the area of Hertwig's epithelial root sheath, but not in the region of the dental papillae. However, dental papillae cells, considered to have the capacity to form cementum, attached to osteopontin coated dishes, in vitro. Thus, unique attachment proteins, as well as those previously identified, were found in mature cementum and during root development. Future studies focused on identifying attachment proteins of mature cementum and determining the spatial and temporal localization of these proteins, pre- and post-cementogenesis, will provide important information necessary for establishing the function of these proteins during root development.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 3","pages":"207-14"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12866235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Salivary pH and lysozyme during oropharyngeal radiotherapy]. [口咽放疗期间唾液pH和溶菌酶]。
Journal de biologie buccale Pub Date : 1990-09-01
A M Fois, M Pourquier, A M Bouisson
{"title":"[Salivary pH and lysozyme during oropharyngeal radiotherapy].","authors":"A M Fois,&nbsp;M Pourquier,&nbsp;A M Bouisson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The pH and lysozyme content of the salivas of 34 patients receiving radiotherapy of the upper respiratory digestive tracts for the treatment of cancer were compared to those of 22 healthy subjects matched for age and gender. After irradiation the salivas of the treated patients had a lower mean pH (p less than 0.001). During radiotherapy no statistically significant changes occurred in the pH or lysozyme rate, but at the beginning of treatment there was an inverse correlation (p less than 0.05) between the increased lysozyme content and the lowered pH. The mean salivary pH and lysozyme rate of 12 patients (35%) initially treated by chemotherapy showed no significant differences from controls. However, the saliva of 21 dentate patients had a mean pH and lysozyme content considerably higher (p less than 0.01) than that of patients without teeth. During radiotherapy there was a statistically significant inverse relationship between the pH of the salivas of the dentate and edentulous groups.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 3","pages":"169-75"},"PeriodicalIF":0.0,"publicationDate":"1990-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13405936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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