Histochemical localization of transforming growth factor-beta 1 in developing rat molars using antibodies to different epitopes.

Journal de biologie buccale Pub Date : 1990-12-01

R N D'Souza, R P Happonen, K C Flanders, W T Butler

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Abstract

In this study transforming growth factor-beta 1 (TGF-beta 1) has been immunolocalized in developing rat molars using two well characterized polyclonal antibodies, Anti-CC and Anti-LC, that recognize extracellular and intracellular TGF-beta 1, respectively. With immunohistochemical methods and the ABC-peroxidase system of detection, the growth factor was immunolocalized within the ectodermally derived enamel organ and the neural crest-derived dental papilla at the early and advanced bell stages of development. With Anti-CC, widespread and abundant extracellular TGF-beta 1 was found associated with the stellate reticulum and within central and apical regions of dental papilla mesenchyme. In contrast, Anti-LC localized TGF-beta 1 intensely within the cells of the outer dental epithelium. Moderate immunostaining for TGF-beta 1 with Anti-LC was also evident within the apical cytoplasm of inner dental epithelial cells and odontoblasts. These findings support the hypothesis that TGF-beta 1 may play a paracrine role in tooth development by regulating the epithelial-mesenchymal interactions that influence growth and cytodifferentiation events.

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转化生长因子- β 1在大鼠磨牙发育中的组织化学定位。

在这项研究中，转化生长因子- β 1 (tgf - β 1)在发育中的大鼠磨牙中被免疫定位，使用两种特性良好的多克隆抗体，Anti-CC和Anti-LC，分别识别细胞外和细胞内的tgf - β 1。采用免疫组织化学方法和abc -过氧化物酶系统检测，在钟形发育早期和晚期外胚层源性牙釉质器官和神经冠源性牙乳头内免疫定位生长因子。在抗cc中，发现广泛和丰富的细胞外tgf - β 1与星状网和牙乳头间质中心和根尖区域有关。相反，Anti-LC将tgf - β 1强烈定位在外牙上皮细胞内。在内牙上皮细胞和成牙细胞的顶端细胞质中，也可见到抗lc对tgf - β 1的中度免疫染色。这些发现支持了tgf - β 1可能通过调节影响生长和细胞分化事件的上皮-间质相互作用在牙齿发育中发挥旁分泌作用的假设。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal de biologie buccale

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