Journal de biologie buccale最新文献

{"title":"How osteoblasts become osteocytes: a decreasing matrix forming process.","authors":"J R Nefussi,&nbsp;J M Sautier,&nbsp;V Nicolas,&nbsp;N Forest","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Osteocyte matrix inclusion process was studied in an in vitro woven bone nodule formation model where a large number of osteocytes at different degrees of maturation were examined. This work focused on early stages of osteocyte inclusion. This matrix inclusion occurred without a matrix synthesis inversion by the future osteocyte and with maintenance of close cell contacts with the replacing cell. A passive matrix embedding process related to a decreased activity of the osteoblast-osteocyte cell is proposed as a comprehensive pathway from osteoblast to osteocyte. The formation of the osteocyte is therefore presented as a very coordinated space and time related cell-cell interaction between cells of the three cell pools of the bone.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 1","pages":"75-82"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13025283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A comparison between 4 subgingival bacteriologic sampling technics].","authors":"M Sixou,&nbsp;D Duffaut-Lagarrigue,&nbsp;J P Lodter","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A comparative study of four classical techniques employed in the sampling of subgingival microflora (paper points, swabbing, curette and washing followed by aspiration) has been carried out. This study was based upon quantitative criteria (number of bacteria sampled) and qualitative criteria (number of morphologically distinct colonies found per sampling technique). Sampling was done on three different groups of patients: a control group, a group of patients with gingivitis and a group of patients with periodontitis. The curette sampling technique was found to be efficient both quantitatively and qualitatively. Difficulties in standardizing this method however were encountered with the failure to achieve reproducible results. For this reason the technique of paper point was preferred. This method was found to be more reliable and reproducible in each of the three groups of patients sampled.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 1","pages":"16-21"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13025554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autometallographic visualization of glycosaminoglycans in the tongue mucosa of rats using cuprolinic blue and enzymatic digestions.","authors":"J P Gokani,&nbsp;D Septier,&nbsp;M Goldberg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In order to visualize by light microscopy the glycosaminoglycans (GAGs) in the rat tongue mucosa, the tissue was fixed with cuprolinic-blue (CB)-aldehyde and the staining enhanced by autometallographic (AM) procedure. As other polyanions were also detected, enzymatic digestions with hyaluronidase, chondroitinase ABC and pronase were performed on these tissues in order to test the specificity of the staining. Chondroitinase ABC caused a dramatic decrease of silver grains in the lamina propria whereas hyaluronidase and pronase induced only discrete or no modification. This supported the concept that the GAGs visualized by CB and autometallography in this area as dermatan sulphate. The other polyanions (mostly DNA and RNA) seen in the epithelial layers were unaffected by these enzyme treatments.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 1","pages":"23-8"},"PeriodicalIF":0.0,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13025555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of HIV infectivity by lactoperoxidase-produced hypothiocyanite.","authors":"M Pourtois,&nbsp;C Binet,&nbsp;N Van Tieghem,&nbsp;P Courtois,&nbsp;A Vandenabbeele,&nbsp;L Thiry","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>HIV aliquots, from a supernatant of ARV-4 cell line, were mixed with an equal volume of a OSCN.generating glucose-glucose oxidase + thiocyanate-lacteroperoxidase solution, and then pre-incubated at 37 degrees C for periods of 30 secs to one hour. These mixtures were then inoculated into cultures of phytohaemagglutinin-stimulated lymphocytes. Viral growth was monitored with an ELISA quantitating the specific p24 protein either in the culture cells or in the supernatant. In control experiments, the virus produced both intra- and extra-cellular p24. In the controls, concentration of p24 per 10(6) lymphocytes grew rapidly. By contrast, HIV that was pretreated with the OSCN generating system for 30 sec., produced very little p24, and no detectable amount of this protein could be found after 2 min. pre-treatment.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"251-3"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13284105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructure of the nuclear bodies in the human parotid gland.","authors":"L Duquene,&nbsp;N Dourov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ultrastructure of 17 human parotid glands was studied in order to determine the features of the nuclear bodies and their possible relation to various pathological conditions. Types I and III nuclear bodies were frequently identified in acini and in striated ducts. Type IV nuclear bodies were seldom found. The percentage of nuclear sections containing nuclear bodies varied from 5.7 to 66.4 for the acini, and from 17.8 to 80% for the striated ducts. In 4 out of our 6 cases of chronic inflammation, numerous nuclear bodies were observed in the same nucleus. The increase in the number of nuclear bodies seemed to be related to an altered cell metabolism. In 3 cases, membranous type inclusions were found. This type of nuclear inclusion is quite different from the true nuclear bodies.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"261-9"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13123310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of acivicin, an inhibitor of gamma-glutamyl transpeptidase on mouse molar development in vitro.","authors":"N P Piesco","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Gamma-glutamyl transpeptidase (GGT) is a membrane-bound enzyme found on the surface of cells having secretory or resorptive functions. GGT has been found to be strongly localized in the stellate reticulum of the developing tooth. It has been proposed that the role for GGT in tooth development is related to the transport of amino acids into the cell via the gamma-glutamyl cycle. In order to ascribe a role for GGT and the stellate reticulum in the developing tooth, the activity of GGT was inhibited by a daily 1 hour application of the glutamine analog, acivicin (Upjohn), to mouse first molar tooth germs in serum free organ culture for periods of 5 to 8 days. Acivicin treatment effectively arrested tooth development. Additionally, tooth germs were allowed to recover for 4 to 7 days following a 4 day treatment with acivicin or they were incubated in media supplemented with additional glutamine or nucleosides during the acivicin treatment. Tooth germs were able to recover when returned to control medium. However, treated teeth were smaller in size than the controls. Glutamine partially compensated for the acivicin treatment. Nucleotide supplemented media appeared to almost completely override the inhibitory effect of acivicin. It appears that the inhibition by acivicin is primarily due to its effect on DNA and RNA synthesis. The inhibition of the gamma-glutamyl cycle by acivicin was unaffected by the addition of glutamine or nucleosides. Therefore, inhibition of the gamma-glutamyl cycle and GGT does not seem to significantly affect the development of teeth at the stages studied.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"313-9"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13141019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proceedings of the 4th workshop on tooth morphogenesis and development (continuation). Arc and Senans, France, May 30-June 2, 1990.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"289-361"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13141018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of a single dose of 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) on presecretory ameloblast differentiation in rat incisors.","authors":"K Josephsen,&nbsp;O Fejerskov,&nbsp;V Baelum,&nbsp;V Weile","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A single, high dose of HEBP to rats results in a triad of lesions along the mineralizing front of the incisor enamel. One type of lesion is a shallow groove crossing the apical enamel surface. The purpose of this study was to explore the pathogenesis of this \"demarcation groove\", and to characterize changes in the involved regions of amelogenesis. Rats were given a subcutaneous dose of 10 mg/kg body weight of HEBP and sacrificed by vascular perfusion at intervals ranging from 1 to 36 hours. Mandibular incisors were processed for light and electron microscopy. The region of ameloblasts facing dentin was divided into two subregions: A region of ameloblasts facing unmineralized dentin, comprising a posterior (Aud/p) and an anterior portion (Aud/a), and a region of ameloblasts facing mineralized dentin (Amd). The progressive apical mineralization of the predentin was arrested up to 12 hours after injection of HEBP, while ameloblasts related to already mineralizing dentin continued to differentiate and secrete enamel matrix. At 8 hours the dentin and enamel layers had assumed a common apical border at the start of Amd, marking the position of the future demarcation groove. The length of Aud/p remained constant, Aud/a doubled in length, and Amd was drastically reduced up to 24 hours after injection of HEBP. The normal migration rate of the ameloblasts was unaffected by HEBP. Accumulations of ameloblast secretory products occurred at certain time intervals between the cell apices, but no morphological changes were recorded in the organelles. Most of the changes observed may be indirect in nature resulting from the physico-chemical effect of HEBP on normal mineralization of dentin and enamel. However, further studies are needed to elucidate possible direct cellular effects on ameloblasts.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"321-37"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13284109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A radioautographic comparison of in vivo 3H-proline and 3H-serine incorporation in the pulpal dentine of rat molars: variations according to the different zones.","authors":"J P Salomon,&nbsp;S Lecolle,&nbsp;M Roche,&nbsp;D Septier,&nbsp;M Goldberg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>3H proline and 3H serine were injected intraperitoneally to rats which were killed 4 and 24 hours later. The incorporation of the labelled precursors was studied in the odontoblasts, predentine and dentine of the first lower molars. After radioautography, statistically significant differences in grain density appeared between the furcation, occlusal and lateral areas of the pulp chamber. The incorporation of predentine components into dentine was faster in the lateral than in the occlusal area, the slowest being detected in the furcation area. These differences may be related to the distribution of occlusal forces in the rat molar.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"307-12"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13284107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of a single dose of 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) on secretory ameloblasts and enamel formation in rat incisors.","authors":"O Fejerskov,&nbsp;K Josephsen,&nbsp;V Weile","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The present experiment was undertaken in order to study how HEBP affects secretory ameloblasts and the mineralizing front of rat incisor enamel resulting in the formation of a hypomineralized incremental band. Rats were given a single subcutaneous injection of 10 mg/kg body weight of HEBP and sacrificed at intervals ranging from 1 hour to 9 days. The incisors were specially prepared for microradiography, scanning electron microscopy, and light and transmission electron microscopy. The microradiographical examination 9 days after injection revealed a distinct incremental band of hypomineralization from the amelo-dentinal junction to the outer enamel surface. The overall rod pattern and mineral distribution within the enamel was otherwise normal. Under light microscopy the ameloblasts exhibited an increase in vacuoles and dark granules in the supranuclear cytoplasm 2-12 hours after injection. At 12 hours a total disarray of the mineralizing front was evident and confirmed by SEM. However, 24 hours after injection a normal structure of the mineralizing front was regained. Ultrastructurally the organelles of the ameloblasts showed no changes from normal at any time interval. However, 2 hours after injection multivesicular bodies appeared frequently in the Tomes' processes and crystal density had diminished in the interrod enamel. At 8 hours a proteinaceous matrixdevoid of crystals was accumulating corresponding to interrod growth regions, whereas no obvious changes were recorded at the rod growth regions. At 12 hours the newly formed interrod enamel was thin and without crystals in some places. In such areas a granular, less-dense matrix was found and a band of small swellings of the interrod enamel was evident most likely corresponding to the time of injection. After 24 hours the cells and the mineralizing front appeared normal except in the interrod growth regions where the cell processes were still separated by wide intercellular spaces in which crystals were occasionally found in a fine granular matrix. These observations are discussed with particular reference on the known physico-chemical effects of HEBP on mineral formation. A possible direct cellular effect on the ameloblasts should be studied using radioautography and immunocytological techniques.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"18 4","pages":"339-54"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13284110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}