{"title":"Rates of protein turnover at specific sites of the rat incisor periodontal ligament.","authors":"J Kirkham, C Robinson, R C Shore","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Extraction of rat incisor periodontal ligament using neutral salt solutions was carried out on a site-specific basis following administration of 3H-labelled proline, the incorporation of 3H-proline into soluble proteins of different molecular weights was determined by SDS electrophoresis and scintillation counting. Tracer was incorporated primarily into 3 fractions with Mr = greater than 120K, 100K and 45-65K. Appearance of 3H-proline in low molecular weight components which cross-reacted with antibodies to Type 1 collagen (presumably collagen breakdown products) within 30 minutes of administration suggests a pool of collagen which is rapidly turning over. The rate of maturation of collagen from this soluble to insoluble forms may represent a level of post-translational control within the tissue which may be directly related to tissue morphostasis.</p>","PeriodicalId":75983,"journal":{"name":"Journal de biologie buccale","volume":"19 1","pages":"61-7"},"PeriodicalIF":0.0000,"publicationDate":"1991-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal de biologie buccale","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Extraction of rat incisor periodontal ligament using neutral salt solutions was carried out on a site-specific basis following administration of 3H-labelled proline, the incorporation of 3H-proline into soluble proteins of different molecular weights was determined by SDS electrophoresis and scintillation counting. Tracer was incorporated primarily into 3 fractions with Mr = greater than 120K, 100K and 45-65K. Appearance of 3H-proline in low molecular weight components which cross-reacted with antibodies to Type 1 collagen (presumably collagen breakdown products) within 30 minutes of administration suggests a pool of collagen which is rapidly turning over. The rate of maturation of collagen from this soluble to insoluble forms may represent a level of post-translational control within the tissue which may be directly related to tissue morphostasis.