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Adrenocorticotropin binding activity of B16/C3 melanoma cells. B16/C3黑色素瘤细胞促肾上腺皮质激素结合活性的研究。
Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163471
J. Simić-Krstić, L. Ševaljević
{"title":"Adrenocorticotropin binding activity of B16/C3 melanoma cells.","authors":"J. Simić-Krstić, L. Ševaljević","doi":"10.1159/000163471","DOIUrl":"https://doi.org/10.1159/000163471","url":null,"abstract":"The binding of adrenocorticotropic hormone (ACTH) to B16/C3 murine melanoma cells was found to be specific and saturable. The binding capacity of the cells changed as a function of the age of the culture. Scatchard analysis revealed one class of high-affinity ACTH binding sites. The specificity of ACTH binding to the cells was tested by displacement experiments with human leukocyte interferon (alpha-IFN) and alpha-melanocyte stimulating hormone (alpha-MSH) as the competitors. Structure-activity relationship of ACTH, alpha-MSH and alpha-IFN was discussed.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 3 1","pages":"138-42"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163471","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64976933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The vertebral column of diabetic sand rats (Psammomys obesus). 糖尿病沙鼠的脊柱。
Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163483
R Silberberg
{"title":"The vertebral column of diabetic sand rats (Psammomys obesus).","authors":"R Silberberg","doi":"10.1159/000163483","DOIUrl":"https://doi.org/10.1159/000163483","url":null,"abstract":"<p><p>Intervertebral discs and vertebral spongiosa of diabetic and nondiabetic sand rats were investigated histologically and morphometrically. In diabetic animals, degeneration of the intervertebral discs was accelerated. Morphometrically, there was a consistent trend in the diabetics toward an attenuated bone turnover, which was, however, statistically significant only in regard to the length of the trabecular surface covered by osteoblasts. In the vertebral column of the sand rat, the tendency to osteoporosis - age-linked or diabetes-related - is reduced apparently due to the influence of local forces which promote osteogenesis.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 4","pages":"217-20"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163483","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14332403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Analysis of cell three-dimensional locomotory vectors. 细胞三维运动载体分析。
Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163494
P B Noble, A Boyarsky
{"title":"Analysis of cell three-dimensional locomotory vectors.","authors":"P B Noble,&nbsp;A Boyarsky","doi":"10.1159/000163494","DOIUrl":"https://doi.org/10.1159/000163494","url":null,"abstract":"<p><p>A method is presented for analysing the vectors of cells locomoting within three-dimensional collagen gels. The method detected differences in locomotory vector patterns between cells locomoting in two different gel formats. The potential of this analytical method for determining the role of the extracellular matrix in modulating cell locomotory behaviour is discussed.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 6","pages":"289-96"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14354785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Dihydroorotic acid dehydrogenase activity in actinomycin-D-treated and normal chick embryos. 放线菌素d处理和正常鸡胚中二氢羟酸脱氢酶的活性。
Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163493
S M Katdare, M Katdare, P N Joshi, L Mulherkar
{"title":"Dihydroorotic acid dehydrogenase activity in actinomycin-D-treated and normal chick embryos.","authors":"S M Katdare,&nbsp;M Katdare,&nbsp;P N Joshi,&nbsp;L Mulherkar","doi":"10.1159/000163493","DOIUrl":"https://doi.org/10.1159/000163493","url":null,"abstract":"<p><p>The effect of actinomycin D on chick embryos cultivated in vitro by New's culturing method was studied. Exposure of chick embryos to actinomycin D (0.05 micrograms/ml) at the primitive streak stage (stage 4; Hamburger and Hamilton) for 6 h showed interference in orotic acid formation. The assay of the enzyme dihydroorotic acid dehydrogenase was carried out in both treated and control embryos. No enzymic activity was observed in actinomycin-D-treated embryos in contrast to the considerable activity in the controls. These observations suggest an interference by actinomycin D in the biogenesis of the enzyme dihydroorotic acid dehydrogenase.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 6","pages":"285-8"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163493","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13987924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cultured liver epithelial cells can incorporate monoclonal antibodies directed against intracellular cytokeratin structures without microinjection. 培养的肝上皮细胞无需显微注射即可加入针对细胞内细胞角蛋白结构的单克隆抗体。
Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163467
S H Swierenga
{"title":"Cultured liver epithelial cells can incorporate monoclonal antibodies directed against intracellular cytokeratin structures without microinjection.","authors":"S H Swierenga","doi":"10.1159/000163467","DOIUrl":"https://doi.org/10.1159/000163467","url":null,"abstract":"<p><p>T51B rat liver cells in the exponential phase of growth were arrested in late G1 by medium calcium deprivation, exposed to mouse monoclonal anti-cytokeratin (Mr = 55,000) by addition of the antibody to the medium, induced to enter the S phase by readdition of 1.5 mM calcium, and incubated for a further 18 h. After fixing and staining with FITC-conjugated anti-mouse IgG, the cells exhibited an intact intracellular cytokeratin-staining pattern. No effect of antibody was observed on entry of cells into the S phase. These results show that rat liver-derived epithelial cells can actively take up macromolecules like cytokeratin antibodies without microinjection.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 1-2","pages":"103-7"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13605016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of thyroid gland activity by hormone assays and flow cytometry in rats. 用激素测定和流式细胞术评价大鼠甲状腺活性。
Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163480
G Zbinden
{"title":"Evaluation of thyroid gland activity by hormone assays and flow cytometry in rats.","authors":"G Zbinden","doi":"10.1159/000163480","DOIUrl":"https://doi.org/10.1159/000163480","url":null,"abstract":"<p><p>Spontaneous variations in the activity of thyroids and changes induced by methimazole, phenobarbital and 2,4--diaminoanisole sulfate were assessed in rats by repeated measurements of serum T3, T4 and TSH concentrations and cell cycle analysis in isolated nuclei of the thyroids. Methimazole caused a decrease in T3 and T4, and elevation in TSH, reduction of cells in G1 and increase in the percentage of cells in S and G2 phases. With phenobarbital T4 concentrations were decreased, but a mitogenic effect was only seen after 8 weeks. With 2,4-diaminoanisole sulfate the most prominent change observed was a decrease in T3 concentrations.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 4","pages":"196-200"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163480","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13608206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Deficiency of a 42-kilodalton protein in tumor-derived fibroblastic cells in neurofibromatosis. 神经纤维瘤病中肿瘤源性成纤维细胞缺乏42千道尔顿蛋白。
Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163473
E Maruyama, A Hayashi, M Arima
{"title":"Deficiency of a 42-kilodalton protein in tumor-derived fibroblastic cells in neurofibromatosis.","authors":"E Maruyama,&nbsp;A Hayashi,&nbsp;M Arima","doi":"10.1159/000163473","DOIUrl":"https://doi.org/10.1159/000163473","url":null,"abstract":"<p><p>Cell proteins obtained from cultured normal appearing skin and neurofibromas of neurofibromatosis patients, and normal skin of normal donors were compared by SDS-PAGE and isoelectric focusing analysis. Essentially, identical protein patterns were obtained for the pellet fractions of all the strains. The lysate fraction binding patterns were also similar to each other, but a deficiency of a 42-kilodalton protein with pI 4.3 was observed in the four tumor-derived cell strains examined. These results raise the possibility that tumor-derived fibroblastic cells are of the same cell origin as skin fibroblasts, and that the deficiency of a 42-kilodalton protein could be related to the tumorigenicity in neurofibromatosis.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 3","pages":"153-8"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163473","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14359439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Studies on thymocyte subpopulations in guinea pigs. VIII. Characterization of a thymocyte population resistant to the cytotoxic effect of normal rabbit serum. 豚鼠胸腺细胞亚群的研究。8抗正常兔血清细胞毒作用的胸腺细胞群的特征。
Experimental cell biology Pub Date : 1988-01-01
G Sandberg, C Stenvinkel, S Kölare
{"title":"Studies on thymocyte subpopulations in guinea pigs. VIII. Characterization of a thymocyte population resistant to the cytotoxic effect of normal rabbit serum.","authors":"G Sandberg,&nbsp;C Stenvinkel,&nbsp;S Kölare","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Guinea pig thymocytes were incubated with normal rabbit serum, which resulted in the death of a great majority of the cells. The remaining rabbit serum-resistant cells, representing less than 10% of the thymocytes, contained euchromatic DNA and were of intermediate size and low density. Functional tests indicated that they were enriched in immunologically mature cells, which responded to the mitogenic lectins phytohemagglutinin and concanavalin A, and were depleted of immature, spontaneously proliferating cells and in cells responding to the thymocyte growth peptide. The described procedure for enrichment of immunologically mature thymus cells in guinea pigs may become useful since glucocorticoid treatment, used in mice for enrichment of mature thymocytes, cannot be used for this purpose in guinea pigs.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 3","pages":"159-68"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14359440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytologic presentation of malignant mesothelioma in pleural effusions. 胸腔积液中恶性间皮瘤的细胞学表现。
Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163482
G Pedio, U Landolt-Weber
{"title":"Cytologic presentation of malignant mesothelioma in pleural effusions.","authors":"G Pedio,&nbsp;U Landolt-Weber","doi":"10.1159/000163482","DOIUrl":"https://doi.org/10.1159/000163482","url":null,"abstract":"<p><p>1,601 pleural effusions were found to be malignant between 1976 and 1987. Among these were 26 (1.6% of the malignant effusions) mesothelioma. Only 2 cases showed pronounced cytologic features that made a definite diagnosis possible on cytologic criteria alone. In 20 cases diagnosis of mesothelioma was strongly suggested by the patient's history and cytology of the effusion was compatible with mesothelioma. In the other 4 cases special examinations (histo- and immunohistochemistry, electron microscopy) led to the final diagnosis. The cytologic features of mesothelioma and other examination techniques, needed to resolve the differential diagnosis of mesothelioma versus other neoplasm in pleural effusions, are discussed.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 4","pages":"211-6"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163482","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14332399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Down-regulation of murine collagen-induced arthritis by a T cell hybridoma. T细胞杂交瘤对小鼠胶原诱导关节炎的下调作用。
Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163466
T F Kresina
{"title":"Down-regulation of murine collagen-induced arthritis by a T cell hybridoma.","authors":"T F Kresina","doi":"10.1159/000163466","DOIUrl":"https://doi.org/10.1159/000163466","url":null,"abstract":"<p><p>T cell hybridoma cell lines were generated by somatic cell fusion of BW 5147 myeloma cells and splenic cells from mice suppressed for collagen-induced arthritis (CIA). Two cell lines were characterized for their cell surface phenotype, antigen recognition and capacity to down-regulate the erythema and edema associated with CIA. Cell line T101N was determined to portray the cell surface phenotype Ly1+2- L3T4- Thy1+ by a direct binding assay. Cell line T104B1 was determined to express only the Thy1+ alloantigen. Panning studies, measurement of IL-2 production in vitro and the suppression of antibodies to type I and type II collagen in vivo indicate that the hybridoma cells are not isotype specific in their recognition of the polymorphic interstitial collagens. Down-regulation of the erythema and edema of CIA occurred on injection of 1 X 10(5) T101N cells but not T104B1 cells. Histology of the tarsus region of the hind paw of CIA mice 33 days after the administration of T101N cells showed contrasting histopathology compared to that of CIA mice. The joints of CIA mice given T101N cells showed aligned articular surfaces resembling normal joint structure and only residual pannus. The data indicate that collagen-specific cloned T cell lines can modulate the gross pathology and joint architecture of joints exhibiting CIA.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 1-2","pages":"86-102"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163466","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14390736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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