{"title":"Systemic anaphylaxis in mast-cell-deficient mice of W/Wv and Sl/Sld genotypes.","authors":"T Y Ha, N D Reed","doi":"10.1159/000163399","DOIUrl":"https://doi.org/10.1159/000163399","url":null,"abstract":"<p><p>Active systemic anaphylaxis was induced in mast-cell-deficient mice of W/Wv and Sl/Sld genotypes. The mast-cell-deficient mice were successfully sensitized either by an intraperitoneal injection of chicken gamma-globulin (C gamma G) mixed with adjuvant, alum and saline extract of Bordetella pertussis, or by an intravenous injection of C gamma G alone. Sensitized mice showed signs of systemic anaphylaxis and died after one or more intravenous challenge injections of C gamma G. These studies show that active systemic anaphylaxis can occur in mast-cell-deficient mice and suggest that cells other than mast cells may release adequate mediators to support systemic anaphylaxis.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 2","pages":"63-8"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163399","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13589345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L Wasserman, J Nordenberg, E Beery, A A Deutsch, A Novogrodsky
{"title":"Differential effects of sodium butyrate and dimethylsulfoxide on gamma-glutamyl transpeptidase and alkaline phosphatase activities in MCF-7 breast cancer cells.","authors":"L Wasserman, J Nordenberg, E Beery, A A Deutsch, A Novogrodsky","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Sodium butyrate and dimethylsulfoxide (DMSO), two known chemical inducers of cell differentiation, were examined on MCF-7 breast cancer cells. Both agents reduce the proliferative capacity of MCF-7 cells, as reflected by inhibition of colony formation in semisolid agar. Sodium butyrate is shown to enhance markedly the activity of two plasma membrane-bound enzymes, alkaline phosphatase and gamma-glutamyl transpeptidase. DMSO does not enhance the activity of these enzymes, but rather induces a small decrease in gamma-glutamyl transpeptidase activity. The present results show that although both agents inhibit cell proliferation, they have a distinct effect on phenotypic expression.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 4","pages":"188-93"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14026645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of dimethylsulfoxide and hexamethylene bisacetamide on the JOK-1 hairy cell leukemia derived cell line propagated in the nude mouse.","authors":"W C Hooper, R F Barth, D P Houchens, R Nines","doi":"10.1159/000163403","DOIUrl":"https://doi.org/10.1159/000163403","url":null,"abstract":"<p><p>The JOK-1 hairy cell leukemia derived cell line has been propagated as a subcutaneous tumor in nude mice. After the tumor had been serially transplanted for at least two successive generations, mice were treated with either dimethylsulfoxide or hexamethylene bisacetamide (HMBA). These agents have been shown to induce terminal leukemic cell differentiation in vitro. Our results indicated that these agents had an in vivo growth inhibitory effect, with HMBA exerting a dose-dependent response. Histopathological examination revealed massive areas of necrosis with no overt signs of cellular differentiation. These data suggest that in vitro inducers of differentiation may act via another mechanism in vivo.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 2","pages":"93-103"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14599579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Interaction of retinoids and tamoxifen on the inhibition of human mammary carcinoma cell proliferation.","authors":"J A Fontana","doi":"10.1159/000163409","DOIUrl":"https://doi.org/10.1159/000163409","url":null,"abstract":"<p><p>The growth of chemically induced mammary tumors is inhibited by both hormone manipulation as well as by retinoids. Numerous mammary carcinoma cell lines are also inhibited by retinoids. Co-treatment of estrogen receptor (ER)-positive breast cancer cells resulted in an additive effect in terms of inhibition of cellular proliferation. The addition of varying concentrations of retinoic acid (RA) to varying concentrations of tamoxifen (TMX) resulted in an additive effect on the inhibition of proliferation of the ER-positive human carcinoma cell lines (MCF-7). Co-treatment of MCF-7 cells over time with RA and TMX resulted in enhanced inhibition of growth. A similar phenomenon was observed when other synthetic retinoids were combined with TMX. This enhanced inhibition by the combination of retinoids and TMX was also observed with other ER-positive cell lines (ZR-75, T47-D), while no effect was noted on the ER-negative cell lines (MDA-MB-231, Hs578T).</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 3","pages":"136-44"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163409","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14786431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Protein synthesis in heterotopically transplanted rat hearts.","authors":"R W Currie, V K Sharma, S M Stepkowski, R F Payce","doi":"10.1159/000163394","DOIUrl":"https://doi.org/10.1159/000163394","url":null,"abstract":"<p><p>This study examines protein synthesis in heterotopically transplanted rat hearts and several tissues of recipient rats. Donor hearts and recipient tissues synthesized many of the normally occurring proteins observed in tissues of unstressed rats. In addition, a stress-induced protein with a molecular mass of 71 kilodaltons was synthesized in donor heart, recipient heart and lung. Donor hearts incorporated more L-[35S]-methionine than did recipient hearts. Tissues of recipient rats also incorporated more label than the respective tissues of sham-recipient rats. These results suggest that ischemia, endured by the donor hearts during transplantation, induced these changes in protein synthesis.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 1","pages":"46-56"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163394","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14675141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vascularization of normal human thyroid tissue transplanted to nude mice.","authors":"J Mölne, E Jörtsö, S Smeds, L E Ericson","doi":"10.1159/000163404","DOIUrl":"https://doi.org/10.1159/000163404","url":null,"abstract":"<p><p>The vascularization of normal human thyroid tissue transplanted to nude, athymic mice was examined by light, electron microscopy and autoradiography after continuous infusion of 3H-thymidine during 2, 4 and 6 days after transplantation. Labelled vascular sprouts were found in the surrounding host connective tissue after 2 days, in between peripheral follicles after 4 days and in central parts of the transplants after 6 days. The autoradiographic observations indicate that the sprouts originated from the surrounding host tissue. The amount of sprouts increased up to a maximum after 2 weeks of transplantation. At this time large interfollicular areas were occupied by sprouts. At later observations (3-5 weeks) sprouts occurred together with typical fenestrated capillaries. After 7 weeks all sprouts had differentiated into mature vessels. Our observations suggest that the transplanted thyroid tissue induces the formation of vascular sprouts in the surrounding host connective tissue. The sprouts then penetrate and vascularize the thyroid tissue.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 2","pages":"104-14"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13589344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphological aspects of interactions between asbestos fibers and human mesothelial cell cytoskeleton.","authors":"J R Rüttner, A B Lang, D R Gut, M U Wydler","doi":"10.1159/000163430","DOIUrl":"https://doi.org/10.1159/000163430","url":null,"abstract":"<p><p>The interaction of chrysotile and amphibole asbestos fibers with the cytoskeleton of cultured human mesothelial cells from nontumoral pleural effusions was studied using scanning electron and immunofluorescence microscopy. Asbestos-exposed mesothelial cells show a massive annular condensation of cytokeratin filaments, forming a concentric ring enveloping the nucleus and the phagocytosed asbestos fibers. By detergent extraction of the cells it could be shown that the asbestos fibers are in close contact with the nuclear membrane and associated with the cytoskeletal framework of the cells. An association of cytokeratin filaments with the asbestos could be observed during phagocytosis of the fibers. The disturbance of the cell cytoskeleton and the close morphologic contact between asbestos fibers and the nuclear membrane may have some relevance in explaining the well-recognized carcinogenic effects of asbestos mineral fibers.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 6","pages":"285-94"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163430","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13598308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Transition from non-muscle to muscle myosin light chains in chick embryo development.","authors":"U Fascio, F De Bernardi","doi":"10.1159/000163426","DOIUrl":"https://doi.org/10.1159/000163426","url":null,"abstract":"<p><p>In chick embryo blastoderm the electrophoretic pattern of myosin light chains changes between the 4-somite and the 19-somite stages (stages 8-13 of Hamburger and Hamilton) from that of non-muscle to muscle myosin. This transition seems to follow the differentiation of the myotomes and to be developmentally regulated.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 5","pages":"271-5"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163426","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14567690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Guanine salvage by organ cultures of mouse tooth germs.","authors":"F J Dye","doi":"10.1159/000163393","DOIUrl":"https://doi.org/10.1159/000163393","url":null,"abstract":"<p><p>The effect of mycophenolic acid (MPA) which inhibits the biosynthesis of guanosine monophosphate (GMP) in organ cultures of mouse tooth germs can be partially counteracted by adding guanine to the MPA cultures. This may be due to salvaging guanine by the enzyme hypoxanthine guanine phosphoribosyl transferase (HGPRT), or to competition for a common membrane carrier involved in mediated transport of both guanine and hypoxanthine in normal biosynthesis and also of MPA. Experiments were carried out to compare the effect of either hypoxanthine or guanine on the MPA-caused inhibition. While addition of guanine to the MPA cultures (MPAG) supports growth equal to controls and development of dental-enamel junction (DEJ) to a level intermediate between control and MPA the addition of hypoxanthine (MPAHX) supports growth and DEJ development not better than MPA. This indicates that guanine is salvaged by HGPRT to GMP while hypoxanthine, salvaged to inosinic acid (inosinic monophosphate, IMP) is ineffective because the MPA inhibition is on the pathway from IMP to GMP.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 1","pages":"42-5"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163393","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14692475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Westphal, M Hänsel, M Brunken, A König, J A Köppen, H D Herrmann
{"title":"Initiation of primary cell cultures from human intracranial tumors on extracellular matrix from bovine corneal endothelial cells.","authors":"M Westphal, M Hänsel, M Brunken, A König, J A Köppen, H D Herrmann","doi":"10.1159/000163411","DOIUrl":"https://doi.org/10.1159/000163411","url":null,"abstract":"<p><p>Tissue specimens from 105 human gliomas and 57 human meningiomas were obtained at surgery, dissociated into single cells and small cell aggregates and then plated onto plain plastic tissue culture dishes and dishes which had been precoated with an extracellular matrix (ECM) derived from bovine corneal endothelium. In 80% of the glioma cases we observed a marked improvement in initial plating efficiency, colony formation and speed of attachment when cells were plated on ECM. In 5 cases cells attached only to the ECM-coated dishes but remained afloat in the untreated dishes. In addition it could be noted that over the first 2 days, those cells which had been initiated on ECM showed more signs of morphological differentiation, i.e., extension of cytoplasmic processes or formation of fiber networks between cell groups. If adaptation occurred and proliferation began in vitro, either immediately or after a several days' lag phase, both the ECM-cultured cells as well as those which slowly had adapted to culture on plastic could be passed on to untreated culture ware and perpetuated thereon. In the case of well-differentiated low-grade gliomas where no growth in culture took place, the cultures on ECM could at least be used for initial experiments in the primary cultures (P0). Meningiomas usually attached well to both, plastic or ECM. In 50% of our cases the plating efficiency was higher on ECM but after successful initial culture, the delay until the cells on plastic reached confluence in comparison with those on ECM was 1 or 2 days. Again there were 2 cases in which the cells would not plate on plastic. Here the cells which after 1 day were still afloat plated to more than 80% within the first 2 h after transfer to ECM. In all cases the cells from plastic and ECM cultures were indistinguishable and could be passed onto untreated dishes henceforth. In later culture stages ECM offers several advantages: It is easier to shift cells to serum-free defined culture conditions, the cells will grow at a faster rate on ECM when in higher passages and the maximal number of passages possible is higher on ECM.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"55 3","pages":"152-63"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13591011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}