Experimental cell biology最新文献

Interaction between endothelial cells and basement membrane components. In vitro studies on endothelial cell adhesion to collagen types I, III, IV and high molecular weight fragments of IV. 内皮细胞与基底膜组分之间的相互作用。内皮细胞与ⅰ型、ⅲ型、ⅳ型及ⅳ型高分子量片段黏附的体外研究。

Experimental cell biology Pub Date : 1989-07-01 DOI: 10.1159/000163544

H. Rixen, C. Kirkpatrick, U. Schmitz, D. Ruchatz, C. Mittermayer

{"title":"Interaction between endothelial cells and basement membrane components. In vitro studies on endothelial cell adhesion to collagen types I, III, IV and high molecular weight fragments of IV.","authors":"H. Rixen, C. Kirkpatrick, U. Schmitz, D. Ruchatz, C. Mittermayer","doi":"10.1159/000163544","DOIUrl":"https://doi.org/10.1159/000163544","url":null,"abstract":"Understanding the mechanisms involved in maintaining the integrity of the vascular endothelium is fundamental to studies on atherosclerosis, thrombosis, inflammation and tumor invasion. One of the essential aspects is the relationship between the endothelial cell (EC) layer and the underlying components of the basement membrane (BM). The importance of the biological role of the individual components of the BM in the promotion of EC adhesion is investigated. In this study suspensions of bovine corneal ECs (BCECs; 5 x 10(4)/ml) were used to investigate the adhesion of EC to collagen type IV and a mixture of fragments of the tetrameric molecule (IV-F, consisting of 75, 120 and 140 kD fragments), as well as collagen types I and III, coated at a 10-micrograms/ml concentration onto glass coverslips in vitro. Adhesion was quantified after 2 h of interaction by direct counting in the light microscope following fixation of the adherent cells. Collagens type IV and IV-F markedly promoted BCEC adhesion both in the presence or absence of 10 or 50% fetal calf serum, indicating that the integrity of the tetrameric molecule is not required for EC adhesion to collagen type IV, but can be replaced by high molecular weight fragments. Collagens type I and III increased EC adhesion in the absence of serum, although not in the presence of serum. Indirect evidence for a possible role of fibronectin in EC adhesion to type-IV collagen is given by the ability of the tetrapeptide (Arg-Gly-Asp-Ser (10 micrograms) to temporarily block (15-30 min) the adhesion-promoting effect of type-IV collagen. The nature of the adhesion sequences on the fragments of type-IV collagen remains to be elucidated.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 6 1","pages":"315-23"},"PeriodicalIF":0.0,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163544","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64977062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Expression of Lewis antigenic determinants in colorectal adenocarcinomas. Lewis抗原决定因子在结直肠癌中的表达。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163519

E Blasco, J Torrado, A Cosme, E Alvarez, A Zugasti, A Gutierrez-Hoyos, J I Arenas

{"title":"Expression of Lewis antigenic determinants in colorectal adenocarcinomas.","authors":"E Blasco, J Torrado, A Cosme, E Alvarez, A Zugasti, A Gutierrez-Hoyos, J I Arenas","doi":"10.1159/000163519","DOIUrl":"https://doi.org/10.1159/000163519","url":null,"abstract":"Expression of type 1 and type 2 chain Lewis antigens was studied in 32 rectal adenocarcinoma specimens; the results were correlated with the patients' Lewis phenotype and secretor status. In addition, the pattern of expression of these antigens was analyzed in adjacent and distant normal mucosa. We used an indirect immunofluorescence technique with p-phenylenediamine counterstaining (Oriol technique) and a panel of monoclonal antibodies directed against the different antigenic specificities. Normal distal colonic mucosa only expresses monofucosylated structures (Lea and X) arising from activity of the alpha 1-3,4-fucosyltransferase coded by the Le gene. Rectal adenocarcinomas also show Lea and X, but also reexpress blood group antigens ABH and exhibit difucosylated determinants (Leb and Y). The accumulation of mono- and difucosylated type 2 chain in neoplastic processes, independently of the Le and Se genes, could be due to the enzymes coded by reactivation of the H and X genes. Blood group antigens form a complex signal code, genetically regulated, which intervenes in differentiation, growth and cellular recognition processes, and which may undergo important modifications during malignant transformation. These alterations could be useful in the diagnosis and prognosis of some types of carcinoma.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 3","pages":"153-8"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163519","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13620690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Cohesive properties of terminally differentiated keratinocytes. 终末分化角质形成细胞的内聚特性。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163509

M M Brysk, S Rajaraman, P Penn, E Barlow, T Bell

引用次数: 7

Formation of double contour as a repair reaction in the human femoral head. 在人股骨头中形成双轮廓作为修复反应。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163540

K Lothe, M A Spycher

引用次数: 1

Expression of the plasma membrane proteolipid in mouse neuroblastoma cells: transient increase in synthesis during differentiation with N6,O2-dibutyryl adenosine 3',5'-cyclic monophosphate. 小鼠神经母细胞瘤细胞质膜蛋白脂的表达:N6, o2 -二丁基腺苷3′，5′-环单磷酸分化过程中合成的短暂增加。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163516

T B Shea, I Fischer

{"title":"Expression of the plasma membrane proteolipid in mouse neuroblastoma cells: transient increase in synthesis during differentiation with N6,O2-dibutyryl adenosine 3',5'-cyclic monophosphate.","authors":"T B Shea, I Fischer","doi":"10.1159/000163516","DOIUrl":"https://doi.org/10.1159/000163516","url":null,"abstract":"We have examined the regulation of plasma membrane proteolipid (PM-PLP) synthesis and steady-state levels in mouse NB2a/d1 neuroblastoma cells during differentiation with dibutyryl cyclic AMP (dbcAMP) and retinoic acid (RA), agents which have been previously shown to induce the elaboration of exclusively axonal or dendritic neurites, respectively. We report that a PM-PLP-immunoreactive species is expressed by this neuroblastoma cell line, and that its expression is regulated by specific states of differentiation. Differentiation of cells with dbcAMP was accompanied by an initial 2-fold increase in this PM-PLP immunoreactive species at 24 h after treatment, which returned to control levels by 96 h after treatment. By contrast, no significant increase in synthesis was detected when cells were treated with RA. Protein blot analysis of PM-PLP in dbcAMP-treated cells indicated that there was little change in its steady-state level until 96 h following treatment, at which time a reduction of 40% was observed. Throughout induced differentiation with dbcAMP, NB2a/d1 cells continued to express a PM-PLP-immunoreactive species which comigrated on immunoblot analysis with PM-PLP form characteristic of embryonic brain (14-16 kDa), and apparently did not express the PM-PLP form characteristic of adult brain (18 kDa).","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 3","pages":"131-8"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163516","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13693727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

In vitro interaction of heterologous platelets with isolated neoplastic cells from a murine mammary gland tumor. 异源血小板与小鼠乳腺肿瘤分离肿瘤细胞的体外相互作用。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163528

A R Eynard, C Falo, P Quiroga, M E Pasqualini

引用次数: 3

Differences in intracellular calcium mobilization by interferon-beta and interferon-gamma in RPMI-4788 cells. 干扰素- β和干扰素- γ对rpm -4788细胞内钙动员的影响。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163510

M Miyake, S Fuchimoto, K Orita

引用次数: 4

Activities of glycolytic enzymes in rapidly proliferating and differentiated C6 glioma cells. 糖酵解酶在快速增殖和分化的C6胶质瘤细胞中的活性。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163520

S G Dastidar, S K Sharma

引用次数: 10

Differential adhesion of metastatic rat mammary carcinoma cells to organ-derived microvessel endothelial cells and subendothelial matrix. 转移性大鼠乳腺癌细胞对器官源性微血管内皮细胞和亚内皮基质的差异粘附。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163518

R B Lichtner, P N Belloni, G L Nicolson

{"title":"Differential adhesion of metastatic rat mammary carcinoma cells to organ-derived microvessel endothelial cells and subendothelial matrix.","authors":"R B Lichtner, P N Belloni, G L Nicolson","doi":"10.1159/000163518","DOIUrl":"https://doi.org/10.1159/000163518","url":null,"abstract":"The in vitro adhesion rates of rat 13762NF mammary adenocarcinoma cell clones of different spontaneous metastatic potentials to cloned microvessel endothelial cell monolayers and their subendothelial extracellular matrix were investigated. In this system, high rates of adhesion of the cloned tumor cell lines to syngeneic target (lung) organ-derived subendothelial matrix correlated with spontaneous metastatic potential, whereas adhesion to the lung microvessel endothelial cell apical surfaces occurred at lower rates and was not highly significantly different among the tumor cell lines. Adhesion rates to bovine aortic large vessel, and human brain and human meningeal microvessel endothelial cell monolayers were, in general, lower than those found with syngeneic lung microvessel endothelial cells, and did not correlate with spontaneous metastatic potential. Growth of endothelial cells in fetal bovine serum or platelet-poor horse serum did not affect the results, suggesting that in this system metastasis-associated organ-adhesive specificity is determined at the level of the subendothelial matrix.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 3","pages":"146-52"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163518","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13915671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Interaction between endothelial cells and basement membrane components. In vitro studies on endothelial cell adhesion to collagen types I, III, IV and high molecular weight fragments of IV. 内皮细胞与基底膜组分之间的相互作用。内皮细胞与ⅰ型、ⅲ型、ⅳ型及ⅳ型高分子量片段黏附的体外研究。

Experimental cell biology Pub Date : 1989-01-01

H Rixen, C J Kirkpatrick, U Schmitz, D Ruchatz, C Mittermayer

{"title":"Interaction between endothelial cells and basement membrane components. In vitro studies on endothelial cell adhesion to collagen types I, III, IV and high molecular weight fragments of IV.","authors":"H Rixen, C J Kirkpatrick, U Schmitz, D Ruchatz, C Mittermayer","doi":"","DOIUrl":"","url":null,"abstract":"Understanding the mechanisms involved in maintaining the integrity of the vascular endothelium is fundamental to studies on atherosclerosis, thrombosis, inflammation and tumor invasion. One of the essential aspects is the relationship between the endothelial cell (EC) layer and the underlying components of the basement membrane (BM). The importance of the biological role of the individual components of the BM in the promotion of EC adhesion is investigated. In this study suspensions of bovine corneal ECs (BCECs; 5 x 10(4)/ml) were used to investigate the adhesion of EC to collagen type IV and a mixture of fragments of the tetrameric molecule (IV-F, consisting of 75, 120 and 140 kD fragments), as well as collagen types I and III, coated at a 10-micrograms/ml concentration onto glass coverslips in vitro. Adhesion was quantified after 2 h of interaction by direct counting in the light microscope following fixation of the adherent cells. Collagens type IV and IV-F markedly promoted BCEC adhesion both in the presence or absence of 10 or 50% fetal calf serum, indicating that the integrity of the tetrameric molecule is not required for EC adhesion to collagen type IV, but can be replaced by high molecular weight fragments. Collagens type I and III increased EC adhesion in the absence of serum, although not in the presence of serum. Indirect evidence for a possible role of fibronectin in EC adhesion to type-IV collagen is given by the ability of the tetrapeptide (Arg-Gly-Asp-Ser (10 micrograms) to temporarily block (15-30 min) the adhesion-promoting effect of type-IV collagen. The nature of the adhesion sequences on the fragments of type-IV collagen remains to be elucidated.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 6","pages":"315-23"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13679076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0