Experimental cell biology最新文献_第2页

Nodular organization and differential intrametastatic distribution of the fluorescent dye Hoechst 33342 in B16 melanoma liver metastasis. 荧光染料Hoechst 33342在B16黑色素瘤肝转移中的结节组织及转移内差异分布。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163534

S F Aliño, E Hilario

{"title":"Nodular organization and differential intrametastatic distribution of the fluorescent dye Hoechst 33342 in B16 melanoma liver metastasis.","authors":"S F Aliño, E Hilario","doi":"10.1159/000163534","DOIUrl":"https://doi.org/10.1159/000163534","url":null,"abstract":"The development of B16F10 liver metastases as related to its vascular organization was studied by morphological and functional methods, using the DNA-binding fluorochrome, Hoechst 33342. Experimental metastases were produced by intrasplenic injection of B16F10 melanoma cells, and the animals were sacrificed 3-7 days after tumor cell injection. The results show that early metastases are made up of avascular nodules of tumor cells, which subsequently developed lacunae which are not lined by endothelial cells and usually contain blood cells. A more developed metastasis seems to be made up of several nodules with or without lacunae. Between the nodules, vessels connected to blood circulation were usually observed. A functional study with a fluorescent dye showed that early metastases stained negatively, while more developed metastases showed a reticular fluorescent pattern, coincident with the intrametastatic vascular network and displaying a nodular image. In this case, the fluorescence displayed a gradient of intensity decreasing from the vessels towards the lacunae, where the tumor cells showed no fluorescence. In summary, our results suggest that B16 liver metastases have a nodular growth pattern, which is characterized by the formation, during their development, of lacunae not connected to the general circulation and that tumor cells have poor access to small molecules delivered from the blood.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 5","pages":"246-56"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163534","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13766164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Attempted modulation of disulfide antitumor activity in Balb/c mice through glutathione depletion. 试图通过谷胱甘肽耗竭调节Balb/c小鼠的二硫抗肿瘤活性。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163537

R K Coshan-Gauthier, D L Kirkpatrick

{"title":"Attempted modulation of disulfide antitumor activity in Balb/c mice through glutathione depletion.","authors":"R K Coshan-Gauthier, D L Kirkpatrick","doi":"10.1159/000163537","DOIUrl":"https://doi.org/10.1159/000163537","url":null,"abstract":"We investigated the effects of buthionine sulfoximine (BSO)-mediated glutathione (GSH) depletion on the antitumor activity in Balb/c mice produced by four disulfide derivatives of 6-TG and 6-MP. Initial studies indicated that 14 h after BSO (5 mmol/kg) injections, tumor GSH levels were maximally depleted, while normal tissue GSH levels had returned to near control levels. Tumor growth delays and growth rates were compared for groups of animals receiving disulfides I-IV with and without BSO administration 14 h previous. Treatments with BSO alone produced no delay or growth rate differences from the control. Compounds II or III administered in the presence and absence of BSO also produced no delay or growth rate differences from control. Compound I (10 mg/kg) alone showed a delay of 5.2 days and a growth rate significantly slower than that of control (p = 0.05). In combination with BSO the effects were not enhanced. Compound IV (50 mg/kg) also produced delays in 2 separate trials (3.1 and 4.8 days) and significantly slower growth rates on each occasion compared to the control (p = 0.05). The growth rates were not significantly lowered in the presence of BSO. Administration of two doses of IV, 4 days apart, produced a delay (4.9 days) similar to that seen with a single dose. It produced 2 cures and was also more toxic, causing 3 deaths. Two doses of IV in combination with BSO pretreatment had a greater delay (16.0 days) and a significantly longer growth rate (p = 0.05) than two doses of IV alone.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 5","pages":"273-80"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163537","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13766167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Behavior of in vitro grown normal human mucosal epithelial cells and tumorigenic rat cells inoculated into nude mice. 体外培养的正常人粘膜上皮细胞和致瘤性大鼠细胞接种裸鼠的行为。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163511

P Holmstrup, D Arenholt-Bindslev, A Jepsen, K Stoltze

引用次数: 1

Alkaline phosphatase expression in human cell lines derived from primary hepatomas. 碱性磷酸酶在人原发性肝癌细胞系中的表达。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163545

T Tokiwa, A Endo, J Sato

引用次数: 5

Cytotoxicity of N6-cycloalkylated adenine and adenosine analogs to mouse hepatoma cells. n6 -环烷基腺嘌呤和腺苷类似物对小鼠肝癌细胞的细胞毒性。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163508

R Thedford, E O Leyimu, D L Thornton, R Mehta