在光学透明电极上电调节细胞形态和细胞骨架的变化。

M Yaoita, M Aizawa, Y Ikariyama
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引用次数: 21

摘要

在光学透明电极(OTE)上研究了HeLa细胞的电调控形态学和细胞骨架的变化,该电极上电位作用的表面细胞行为和形态发生很容易观察到。在+0.5 V以上(vs. Ag/AgCl)和0 V以下的电位作用下,OTE中的HeLa细胞表现出显著的形态变化。在该电位范围内的每个电位处,使用荧光探针(罗丹明phalloidin)观察f -肌动蛋白分布的变化。这些结果表明,电场诱导亚细胞的细胞骨架变化。电刺激细胞与OTE可以是一个有价值的策略,操作培养的人类细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electrically regulated cellular morphological and cytoskeletal changes on an optically transparent electrode.

Electrically regulated morphological and cytoskeletal changes of HeLa cells were studied on an optically transparent electrode (OTE), on which potential-applied surface cellular behavior and morphogenesis were easily observed. Upon application of a potential, HeLa cells in an OTE exhibited remarkable morphological changes above +0.5 V (vs. Ag/AgCl) and below 0 V. At each potential in this potential range, change in F-actin distribution was observed using a fluorescent probe (rhodamine phalloidin). These results suggest that an electrical field induces a subcellular cytoskeletal change. Electrostimulation of cells with OTE can be a valuable strategy for the manipulation of cultured human cells.

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