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In vitro proliferation of murine spleen cells: inhibition by monoclonal antibodies to L3T4 and Lyt-2 T cell markers or intracellular cyclic adenosine monophosphate. 小鼠脾细胞体外增殖:L3T4和Lyt-2 T细胞标记物单克隆抗体或细胞内环磷酸腺苷单克隆抗体的抑制作用。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163548
P Zhou, T Gorzynski, W K Dowjat, R Rabin, M B Zaleski
{"title":"In vitro proliferation of murine spleen cells: inhibition by monoclonal antibodies to L3T4 and Lyt-2 T cell markers or intracellular cyclic adenosine monophosphate.","authors":"P Zhou,&nbsp;T Gorzynski,&nbsp;W K Dowjat,&nbsp;R Rabin,&nbsp;M B Zaleski","doi":"10.1159/000163548","DOIUrl":"https://doi.org/10.1159/000163548","url":null,"abstract":"<p><p>Proliferation of normal (not immunized intentionally) murine spleen cells was elicited with concanavalin A, supernatant fluid from cultures of EL-4 cells, human recombinant interleukin 2 (IL-2), or a mixture of phorbol ester and calcium ionophore A23187. IL-2-induced proliferation was inhibited by membrane-permeable dibutyryl cyclic adenosine monophosphate (cAMP) or by the adenylate cyclase activator forskolin. Consistent with these observations was the finding that stimulation with IL-2 decreased and forskolin increased the intracellular content of cAMP. IL-2-induced proliferation, as well as that induced by concanavalin A or phorbol-ionophore mixture, was inhibited by monoclonal antibodies specific for L3T4 or Lyt-2 cell surface markers. This inhibition was observed even when antibodies were added several hours after exposure of cells to IL-2. Notably, antibodies did not alter the intracellular content of cAMP. Thus, the experimental data failed to establish a functional linkage between the inhibitory effect of antibodies and the regulatory effect of the adenylate cyclase system. However, our results provide a rational basis for the postulation that antibodies, upon binding to their corresponding ligands, generate a negative signal that interferes with IL-2-induced proliferation. Therefore, L3T4 and Lyt-2 molecules appear to play an important role in the regulation of lymphocyte proliferation.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 6","pages":"346-57"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163548","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13705931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Review of the significance of pulmonary intravascular macrophages with respect to animal species and age. 肺血管内巨噬细胞在动物种类和年龄方面的意义。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163539
G C Winkler
{"title":"Review of the significance of pulmonary intravascular macrophages with respect to animal species and age.","authors":"G C Winkler","doi":"10.1159/000163539","DOIUrl":"https://doi.org/10.1159/000163539","url":null,"abstract":"<p><p>Pulmonary intravascular macrophages (PIMs) have been recognized as the site of substantial uptake of blood-borne particles in the lungs of a number of domestic animal species. Concomitantly, there is a pronounced lung susceptibility to endotoxin in calves, goats, sheep, pigs, and cats. Hemodynamic changes and initial lung injury after endotoxin administration are mediated by arachidonic acid metabolites from a pulmonary source. A significant role of PIMs in regulating pulmonary hemodynamics is implicated.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 6","pages":"281-6"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163539","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13665969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
Generalized cutaneous hyperpigmentation in hairless mice induced by topical dimethylbenzanthracene. 外用二甲基苯并蒽致无毛小鼠全身皮肤色素沉着。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163541
D Tsambaos, F Sampalis, H Berger
{"title":"Generalized cutaneous hyperpigmentation in hairless mice induced by topical dimethylbenzanthracene.","authors":"D Tsambaos,&nbsp;F Sampalis,&nbsp;H Berger","doi":"10.1159/000163541","DOIUrl":"https://doi.org/10.1159/000163541","url":null,"abstract":"<p><p>The skin of hairless (Ng/Bln) mice topically treated with dimethylbenzanthracene (DMBA) was investigated by light microscopy, histochemistry, electron microscopy and autoradiography in order to gain some insight into the mechanisms by which the DMBA-induced cutaneous hyperpigmentation is mediated. The results of the present study indicate that this phenomenon is due to the DMBA-induced stimulation of both the tyrosinase system and the mitotic activity of dopa-inactive dormant melanocytes.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 6","pages":"292-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163541","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13665971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Fibronectin content of the annulus fibrosus in diabetic and non-diabetic sand rats. 糖尿病大鼠和非糖尿病大鼠纤维环纤维连接蛋白的含量。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163531
R Silberberg, W Meier-Ruge, B Odermatt
{"title":"Fibronectin content of the annulus fibrosus in diabetic and non-diabetic sand rats.","authors":"R Silberberg,&nbsp;W Meier-Ruge,&nbsp;B Odermatt","doi":"10.1159/000163531","DOIUrl":"https://doi.org/10.1159/000163531","url":null,"abstract":"<p><p>Annulus fibrosus of intervertebral discs from diabetic and non-diabetic sand rats were examined by microspectrophotometry for fibronectin content. This was higher in the diabetic animals both in the dorsal and ventral parts and in the outer and inner lamellae of the annulus. It is suggested that diabetes-related changes in fibronectin are similar to changes in annular collagen observed in species other than sand rats.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 5","pages":"229-32"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163531","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13836976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced survival of motoneurons in the chick lateral motor column: effects of embryonic skeletal muscle extracts and myoblast-conditioned medium. 增强鸡外侧运动柱运动神经元的存活:胚胎骨骼肌提取物和成肌细胞条件培养基的作用。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163515
G Petruzzelli, W F Hughes
{"title":"Enhanced survival of motoneurons in the chick lateral motor column: effects of embryonic skeletal muscle extracts and myoblast-conditioned medium.","authors":"G Petruzzelli,&nbsp;W F Hughes","doi":"10.1159/000163515","DOIUrl":"https://doi.org/10.1159/000163515","url":null,"abstract":"<p><p>Muscle-derived factors have shown neurotrophic effects in culture, but their possible effects on the maintenance of embryonic motoneurons have not been demonstrated in vivo. Soluble extracts derived from embryonic chick muscle, or medium conditioned by chick myoblasts, were instilled onto the chorioallantoic membrane (CAM) between the 5th and 11th days of incubation. Counts of the lumbar lateral motor column (LMC) at embryonic day 12 revealed modest but significant increases (12-15%) in motoneuron number for these experimental groups as compared with control treatments. The results suggest that sustaining effects of muscle-derived factors on motoneurons may be demonstrated on the developing LMC by the simple expedient delivery via the CAM, and that these factors can modify the normal program of cell death occurring during this critical period of development.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 3","pages":"125-30"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163515","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13915669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The role of laminin in cancer invasion and metastasis. 层粘连蛋白在肿瘤侵袭和转移中的作用。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163521
G Hunt
{"title":"The role of laminin in cancer invasion and metastasis.","authors":"G Hunt","doi":"10.1159/000163521","DOIUrl":"https://doi.org/10.1159/000163521","url":null,"abstract":"<p><p>Laminin, a basement membrane glycoprotein, has been implicated in a number of stages in tumour invasion and metastasis. In addition to its roles in cell adhesion and migration, laminin may be important in mediating interactions of tumour cells with the immune system and have more subtle roles in controlling metastatic behaviour. Recent work on the prognostic significance of laminin and the possible role of the molecule in antimetastasis therapy will also be discussed.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 3","pages":"165-76"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163521","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13816365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 48
Hypoxic injury of rat cortical neurons in primary cell cultures. Introduction of a modified method to create the hypoxic state. 原代细胞培养大鼠皮层神经元的缺氧损伤。介绍一种改进的创建缺氧状态的方法。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163543
A Kinoshita, K Yamada, T Hayakawa
{"title":"Hypoxic injury of rat cortical neurons in primary cell cultures. Introduction of a modified method to create the hypoxic state.","authors":"A Kinoshita,&nbsp;K Yamada,&nbsp;T Hayakawa","doi":"10.1159/000163543","DOIUrl":"https://doi.org/10.1159/000163543","url":null,"abstract":"<p><p>A primary culture of cortical neurons was established from a 17-day-old rat embryo. Twenty-four hours after plating (day 0), the medium was changed to a chemically defined one. Hypoxia was exerted for 4 h by placing cultures in an air-tight chamber connected to a vacuum pump and a cylinder filled with 95% N2 and 5% CO2. Hypoxic stress was exerted on one of the consecutive days from day 0 to day 3. Hypoxia on days 0, 1 or 2 reduced the number of surviving neurons immediately following exposure. However, hypoxia on day 3 resulted in delayed reduction. Anit-synaptophysin antibody was used immunocytochemically to examine whether the difference is related to teh synaptic formation of cultured neurons. Neurons which had more and longer neurites were clearly stained for synaptophysin. We suspect that neurons with synaptic formation release substances from their presynaptic vesicles, and that these substances might account for the delay reduction.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 6","pages":"310-4"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163543","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13664337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Variations in WGA-binding proteins in tumours growing in different implantation sites. 不同植入部位肿瘤生长中wga结合蛋白的变化。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163502
W S Chan, C M Page, G A Turner
{"title":"Variations in WGA-binding proteins in tumours growing in different implantation sites.","authors":"W S Chan,&nbsp;C M Page,&nbsp;G A Turner","doi":"10.1159/000163502","DOIUrl":"https://doi.org/10.1159/000163502","url":null,"abstract":"<p><p>The protein/glycoprotein composition of Triton X100 extracts of four metastatic tumour lines have been compared by gel electrophoresis after the implantation into different sites of the body. No consistent differences in the patterns were noted after staining with Coomassie Blue or treating with concanavalin A. In contrast, after treating with wheat germ agglutinin (WGA), the patterns varied with site. This was most marked with the ML and M5 tumours, where the overall binding was much lower for some sites. Although WGA binding with the LL and BL6 tumours was generally higher than with the ML and M5, reduced binding was still evident for some sites. Reduced WGA binding was also observed for extracts of secondary tumour growing in the liver or lungs.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 1","pages":"11-8"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163502","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13618620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunoglobulin and T cell receptor gene rearrangements in lymphoproliferative disorders. 淋巴细胞增生性疾病中的免疫球蛋白和T细胞受体基因重排。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163523
H Daus, G Schwarze, H Pees, H Radtke, G Kümel, P G Scheurlen
{"title":"Immunoglobulin and T cell receptor gene rearrangements in lymphoproliferative disorders.","authors":"H Daus,&nbsp;G Schwarze,&nbsp;H Pees,&nbsp;H Radtke,&nbsp;G Kümel,&nbsp;P G Scheurlen","doi":"10.1159/000163523","DOIUrl":"https://doi.org/10.1159/000163523","url":null,"abstract":"<p><p>Thirty-one samples representing Hodgkin's and non-Hodgkin's lymphomas, angioimmunoblastic lymphadenopathy (AILD), and benign follicular hyperplasia in HIV infections were examined for rearrangements of the immunoglobulin (Ig) and T cell receptor (TcR) beta-chain gene loci. In 11 of 12 non-Hodgkin's lymphomas (classified as Burkitt lymphoma (2), centrocytic lymphoma (1), centrocytic-centroblastic lymphoma (5), centroblastic lymphoma (3], only rearranged Ig genes could be detected. The exceptional case was an unclassified high-grade lymphoma, which represented a rearrangement of the TcR beta-chain. We also examined DNA from lymphoid neoplasms in which the lineage of the malignant cell was still controversial. Rearrangement of the TcR could exclusively be demonstrated in all 3 cases of AILD. One Ig gene rearrangement and 4 TcR beta-chain rearrangements were found in 13 samples of Hodgkin's lymphomas (11 lymph nodes, 1 pleura effusion and 1 bone biopsy with proven infiltration). Examination of 3 cases of benign follicular hyperplasia in HIV infection represented one Ig rearrangement.</p>","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 4","pages":"177-84"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163523","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13701525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Nuclear retinoic-acid-binding proteins and receptors in retinoic-acid-responsive cell lines. 视黄酸反应细胞系中的核视黄酸结合蛋白和受体。
Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163547
A K Daly, J L Rees, C P Redfern
{"title":"Nuclear retinoic-acid-binding proteins and receptors in retinoic-acid-responsive cell lines.","authors":"A K Daly,&nbsp;J L Rees,&nbsp;C P Redfern","doi":"10.1159/000163547","DOIUrl":"https://doi.org/10.1159/000163547","url":null,"abstract":"Nuclear retinoic-acid-binding activity and the expression of retinoic acid receptor mRNA (RAR-alpha and RAR-beta) were assayed in the F9 embryonal carcinoma, HeLa, HL-60 promyelocytic leukaemia and S91 melanoma cell lines. A 4-svedberg nuclear retinoic-acid-binding activity was detected in all 4 cell lines, but the levels in the HeLa and HL-60 cells were lower than in the F9 and S91 lines. RAR-alpha mRNA was expressed in all 4 cell lines, although at a very low level in S91 cells. Conversely, RAR-beta mRNA was expressed in S91 cells and, at a lower level, in F9 cells but was undetectable in HeLa and HL-60 cells. RAR-beta, transcribed and translated in vitro from the cloned cDNA coding region, sedimented at 4 S and this suggests that the 4-svedberg nuclear retinoic-acid-binding activity may represent the retinoic acid receptors.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 6","pages":"339-45"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163547","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13705930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
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