Experimental cell biology最新文献_第4页

Immunolocalization of a subnucleolar nucleoprotein complex containing RNA polymerase 1 in ascites hepatoma cells using monoclonal antibodies. 利用单克隆抗体在腹水肝癌细胞中免疫定位含有RNA聚合酶1的亚核仁核蛋白复合物。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163546

N L Rosenberg, G L Nicolson

{"title":"Immunolocalization of a subnucleolar nucleoprotein complex containing RNA polymerase 1 in ascites hepatoma cells using monoclonal antibodies.","authors":"N L Rosenberg, G L Nicolson","doi":"10.1159/000163546","DOIUrl":"https://doi.org/10.1159/000163546","url":null,"abstract":"We have isolated a discrete subnucleolar macromolecular nucleoprotein complex by direct treatment of Novikoff ascites hepatoma nucleoli by MspI restriction digestion. Using a monoclonal antibody made against the subnucleolar nucleoprotein complex that was shown to inhibit RNA polymerase (pol) 1 activity in vitro, we localized an Mr approximately 55,000 protein subunit which was demonstrated previously by an enzyme-linked immunosorbent assay and Western blotting to share epitopes with the RNA pol 1 moiety of the subnucleolar complex. By indirect immunofluorescence the distribution of the Mr approximately 55,000 component of the subnucleolar nucleoprotein complex was examined at various phases of the cell cycle. At prophase, it was localized in large (approximately 1.5 microns in diameter) ball-like structures associated with the nuclear periphery and nuclear peripheral chromatin, suggesting that these structures might be related to preribosomal elements. After chromatin condensation and the pairing of daughter chromosomes, the large ball-like spheres increased in size and were associated with propidium iodide staining at one side of the nucleus; whereas throughout and especially at the opposite side of the nucleus, smaller, round, punctate structures of approximately 0.5 micron in diameter were visibly labeled that were not associated with propidium iodide staining. At later stages of the cell cycle, these small round structures were again associated with propidium iodide staining, suggesting that they may be related to prenucleolar and/or preribosomal elements which would likely contain the appropriate nucleic acid in association with RNA pol 1 and cofactors of RNA pol 1.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 6","pages":"330-8"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163546","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13664338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell surface changes during electromagnetic field exposure. 电磁场作用下细胞表面的变化。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163501

S H Hamada, R Witkus, R Griffith

引用次数: 12

Characterization and differential expression of an endothelial cell-specific surface antigen in continuous and sinusoidal endothelial, in skin vascular lesions and in vitro. 内皮细胞特异性表面抗原在连续和正弦内皮、皮肤血管病变和体外的特征和差异表达。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163524

S Goerdt, F Steckel, K Schulze-Osthoff, H H Hagemeier, E Macher, C Sorg

{"title":"Characterization and differential expression of an endothelial cell-specific surface antigen in continuous and sinusoidal endothelial, in skin vascular lesions and in vitro.","authors":"S Goerdt, F Steckel, K Schulze-Osthoff, H H Hagemeier, E Macher, C Sorg","doi":"10.1159/000163524","DOIUrl":"https://doi.org/10.1159/000163524","url":null,"abstract":"Continuous and sinusoidal endothelial cells display marked morphological and functional heterogeneity as to their plasmalemmal vesicle content, to the kind of intercellular junctional complexes, to the existence and kind of fenestrae and gaps, to the existence and character of their basement membrane, to their ability for phagocytosis and to other functional parameters. Monoclonal antibody 1F10, raised against human umbilical vein endothelial cells (HUVE cells), reflects these differences in recognizing--without any nonendothelial side reactions--an endothelial cell surface antigen, abundantly expressed in continuous endothelia, low and inconsistently expressed in liver sinusoidal and dermal lymphatic endothelia and absent from splenic sinusoidal endothelial cells. In differentiated skin vascular tumors, 1F10 antigen is expressed in normal amounts while it is only low and inconsistently expressed in the dedifferentiated endothelial cells of Kaposi's sarcoma and hemangiosarcoma. HUVE cells in culture, in contrast to their in situ ancestors, express variable amounts of 1F10 antigen. When endothelial cell-conditioned medium (ECC medium) is supplied to HUVE cells in culture, no 1F10 antigen is expressed, while supplementation with fresh serum-containing medium (FSC medium) or cytokines, such as bFGF, suffices to maintain 1F10 expression in 10-70% of the cells. From this we conclude that developmental regulation, environmental influences and cytokine supply contribute to the differentiation and maintenance of the 1F10+ and 1F10-endothelial cell phenotypes, both in vivo and in vitro.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 4","pages":"185-92"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163524","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13741212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Vascularization of ovaries from golden hamsters following implantation into the chick chorioallantoic membrane. 植入雏鸡绒毛尿囊膜后，金仓鼠卵巢血管化。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163529

K Spanel-Borowski

{"title":"Vascularization of ovaries from golden hamsters following implantation into the chick chorioallantoic membrane.","authors":"K Spanel-Borowski","doi":"10.1159/000163529","DOIUrl":"https://doi.org/10.1159/000163529","url":null,"abstract":"In order to study the angiogenic activity, entire ovaries of immature or adult golden hamsters in different functional stages were implanted into the chick chorioallantoic membrane (CAM). Ovaries or other organs (heart, lung, liver, spleen, kidney, adrenal gland, testis) were applied during days 9-14 of incubation. Histological serial sections and ultrastructural studies displayed vascularization from the host to the ovary. Vascularization was marked for unstimulated immature ovaries compared to superstimulated immature ovaries or adult ovaries obtained during the period of proestrus-estrus. When no vascularization developed, grafted ovaries of immature or adult animals induced a mild erosive reaction of the CAM. In contrast, few other implanted organs obtained from immature animals caused a response with vascularization which was absent in grafted organs from adult hamsters. The erosive reaction of the CAM was rare, but the ulcerative response accompanied by infiltrating eosinophils was frequently seen both in immature and adult animals. These results may indicate that angiogenetic activity of the ovary is not the sole cause for vascularization of grafts. Other factors such as tissue death or a low histocompatibility barrier are likely to be involved.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 4","pages":"219-27"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13627344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Further characterization of the biological properties of human hematopoietic survival and growth factor. 进一步表征人造血生存与生长因子的生物学特性。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163504

A Hiraoka, T Ohkubo, M Fukuda

引用次数: 2

Time-based changes in fibroblast three-dimensional locomotory characteristics and phenotypes. 成纤维细胞三维运动特征和表型的时间变化。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163533

P B Noble, E D Shields

引用次数: 9

Changes in collagen synthesis by human bone marrow fibroblasts with progressive subcultivation. 进行性继代培养人骨髓成纤维细胞胶原合成的变化。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163535

M Fernandez, C Barahona, J Martinez, J J Minguell

引用次数: 3

Studies on thymocyte subpopulations in guinea pigs. X. Rosette-forming ability of thymocyte subpopulations before and after incubation in vitro. 豚鼠胸腺细胞亚群的研究。X.体外培养前后胸腺细胞亚群的莲座形成能力。

Experimental cell biology Pub Date : 1989-01-01

S Kölare, G Sandberg

{"title":"Studies on thymocyte subpopulations in guinea pigs. X. Rosette-forming ability of thymocyte subpopulations before and after incubation in vitro.","authors":"S Kölare, G Sandberg","doi":"","DOIUrl":"","url":null,"abstract":"Thymocytes spontaneously proliferating in vitro were labelled with 3H-thymidine, and the distribution of label among rosette-forming cells (RFC+) and nonrosetting cells (RFC-), as well as in populations differing in buoyant density, was measured by liquid scintillation counting and autoradiography before and after incubation for 24 h. Initially most labelled cells (88%) belonged to the low-density (1a) subpopulation, the majority being RFC+. After incubation for 24 h, low-density nonrosetting thymocytes (1a, RFC-) contained the highest amount of label. A decreased rosette formation occurred not only in labelled cells but also in the population as a whole, and in separately incubated high-density cells. The decreased rosette formation was mainly caused by a change in rosette-forming ability of viable high-density cells, however in part also by decreased viability. A shift from low to high density occurred among labelled cells during incubation and was shown to occur in both RFC+ and RFC-. The decreased rosette formation of labelled cells during in vitro culture contrasts with the increase earlier observed in vivo and may therefore represent affinity alterations or a down-regulation of the rosette receptor in vitro. We conclude that the observed changes in density, but not in rosette-forming ability, may reflect normal differentiation.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"57 5","pages":"264-72"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13766166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Membrane fluidity and cancer metastasis. 膜流动性与肿瘤转移。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163526

G V Sherbet

引用次数: 55

Partial purification of human urinary megakaryocyte colony-stimulating factor. 人尿巨核细胞集落刺激因子的部分纯化。

Experimental cell biology Pub Date : 1989-01-01 DOI: 10.1159/000163503

K Ogata, S Kuriya, K Dan, T Nomura

引用次数: 7