Experimental cell biology最新文献_第7页

Origin of cells in human oral mucosa heterotransplants in nude mice. 裸鼠口腔黏膜异源移植细胞来源的研究。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163468

P Holmstrup, N Gram

引用次数: 0

Growth factor effects on small cell lung cancer cells using a colorimetric assay: can a transferrin-like factor mediate autocrine growth? 生长因子对小细胞肺癌细胞的比色效应:转铁蛋白样因子能否介导自分泌生长?

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163465

Y Nakanishi, F Cuttitta, P G Kasprzyk, I Avis, S M Steinberg, A F Gazdar, J L Mulshine

{"title":"Growth factor effects on small cell lung cancer cells using a colorimetric assay: can a transferrin-like factor mediate autocrine growth?","authors":"Y Nakanishi, F Cuttitta, P G Kasprzyk, I Avis, S M Steinberg, A F Gazdar, J L Mulshine","doi":"10.1159/000163465","DOIUrl":"https://doi.org/10.1159/000163465","url":null,"abstract":"A semiautomated colorimetric assay (MTT assay), based on the ability of live cells to reduce a tetrazolium-based compound, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), to a purplish colored formazan product that can be measured spectrophotometrically, has recently been adapted for use in drug sensitivity analysis of cultured human tumor cell lines. We report the application of this assay for the evaluation of the growth factor requirements of human small cell lung cancer (SCLC) cell lines. Specifically, the growth stimulation of each constituent of a previously reported serum-free defined medium system for SCLC including various concentrations of hydrocortisone, insulin, transferrin, 17 beta-estradiol, and selenium (HITES) was evaluated. The optimal concentrations for insulin, transferrin, and selenium derived in the previously reported experiments with direct counting of viable cells were similar to optimal concentrations determined for the growth of three SCLC cell lines (NCI-H82, NCI-N417, NCI-H526) using the MTT assay. In contrast to the previous report, the growth-stimulating effects of hydrocortisone and 17 beta-estradiol were negligible. Using the MTT we have shown that a SCLC cell line, NCI-H345 (which has been previously reported to produce a transferrin-like molecule), was growth-inhibited by an anti-transferrin receptor antibody, when grown in transferrin-free media. The conditioned media from this cell line is stimulatory to other transferrin-sensitive cell lines, suggesting the possibility of an autocrine role for this transferrin-like molecule at least in that cell line. With carefully defined conditions for a given cell line in which cell density and other parameters are within a range of constant MTT metabolism, the assay is well suited for precise analysis of growth factor effects.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 1-2","pages":"74-85"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163465","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13982866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Expression of T cell receptor gamma-chain in murine cytotoxic T cells: analysis of a highly transcribed nonrearranged gene cluster. 小鼠细胞毒性T细胞中T细胞受体γ链的表达:一个高度转录的非重排基因簇的分析。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163478

M Schulz, G Lienhard, F Rupp, R M Zinkernagel, H Hengartner

{"title":"Expression of T cell receptor gamma-chain in murine cytotoxic T cells: analysis of a highly transcribed nonrearranged gene cluster.","authors":"M Schulz, G Lienhard, F Rupp, R M Zinkernagel, H Hengartner","doi":"10.1159/000163478","DOIUrl":"https://doi.org/10.1159/000163478","url":null,"abstract":"The murine T cell receptor gamma-gene family is organized in 4 gene clusters which encode variable, joining and constant gene segments. Because the function of the gamma-gene products is still unclear, there is great interest in functional rearrangements of these genes. We searched for new variable gamma-gene segments in the gamma 4-gene cluster. The mapping and sequencing of a supposed new variable segment of a functionally spliced transcript from a cDNA library of the cytotoxic T cell clone 3F9 was attempted. The results show that the supposed variable segment is a transcribed noncoding germline sequence 5' adjacent to the gamma 4-joining segment. With the gamma 4-constant sequence as a probe, strong signals could be shown in Northern analysis of the original T cell clone 3F9 and in other virus-specific T cell clones. Since all the T cell clones investigated did not show any rearrangement in the gamma 4-gene cluster, the observed high transcriptional activity in this region could be involved functionally or as an intermediate in T cell receptor rearrangement.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 4","pages":"181-9"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163478","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13987474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Differentiation of mononuclear blood cells into macrophages, fibroblasts and endothelial cells in thrombus organization. 血栓组织中单核血细胞向巨噬细胞、成纤维细胞和内皮细胞的分化。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163481

H J Leu, W Feigl, M Susani, B Odermatt

引用次数: 35

Human trophoblast glycoproteins defined by monoclonal antibody 1D2. 单克隆抗体1D2定义的人滋养细胞糖蛋白。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163461

N Hole, N Barton-Hanson, S Berwick, P Stern

引用次数: 1

Megakaryocyte colony-stimulating factor and burst-promoting activity in LPS-treated mouse spleen cell-conditioned medium. lps处理小鼠脾细胞条件培养基中巨核细胞集落刺激因子和促爆发活性。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163487

M Sugimoto, Y Wakabayashi, S Hirose, M J Murphy

引用次数: 1

Progressive loss of neuronal src protein in postnatal weaver and staggerer cerebellum. 出生后织布和交错小脑中神经元src蛋白的进行性丢失。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163479

O D Wiestler, E Trenkner, G Walter

引用次数: 5

Festschrift J.R. Rüttner. Rüttner。

Experimental cell biology Pub Date : 1988-01-01

引用次数: 0

Characteristics of cyclic AMP enhancement of retinoic acid induction of increased transglutaminase activity in HL60 cells. 环AMP增强维甲酸诱导HL60细胞转谷氨酰胺酶活性升高的特性。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163462

A M Maddox, M K Haddox

{"title":"Characteristics of cyclic AMP enhancement of retinoic acid induction of increased transglutaminase activity in HL60 cells.","authors":"A M Maddox, M K Haddox","doi":"10.1159/000163462","DOIUrl":"https://doi.org/10.1159/000163462","url":null,"abstract":"When the human myeloid leukemia cell line (HL60) is induced to differentiate with retinoic acid (RA), there is a concentration-dependent increase in transglutaminase (TGase) activity which peaks on day 5. While dibutyryl 3',5'-cyclic adenosine monophosphate (db-cAMP) alone produced only a slight increase in TGase activity in HL60 cells, the concomitant addition of db-cAMP (100 microM) with RA (10(-12)-10(-4) M) potentiates RA induction of TGase activity. Maximal increases in TGase activity (2- to 10-fold) were observed with 10(-4)-10(-7) M RA and when db-cAMP was present from 24 to 48 h after the addition of RA. The cyclic nucleotide enhancement was dose-dependent from 10 to 100 microM of cAMP. Less marked increases were observed with 8-bromo-cAMP and with the phosphodiesterase inhibitor theophylline. Although the simultaneous addition of PGE1 or PGE2 (10(-8)-10(-6) M) produced no enhancement of RA-induced TGase activity, adding PGE1 or PGE2 24 or 48 h following RA treatments produced an enhancement of TGase activity. The phosphodiesterase inhibitor potentiated the increases produced by db-cAMP and the prostaglandins. Dibutyryl cAMP enhanced the ability of RA to induce the cells to reduce nitroblue tetrazolium (NBT), a functional measure of differentiation, at lower concentrations of RA and with shorter treatment durations. cAMP potentiates RA-induced TGase activity in HL60 cells and the combination appears to be associated with enhanced RA-induced differentiation.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 1-2","pages":"49-59"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163462","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14039406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The role of antibodies in natural and acquired resistance of mice to vesicular stomatitis virus. 抗体在小鼠对水泡性口炎病毒的自然和获得性抵抗中的作用。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163477

R Gobet, A Cerny, E Rüedi, H Hengartner, R M Zinkernagel

引用次数: 74