Experimental cell biology最新文献_第6页

Extracellular materials and determination of neuroectoblast in amphibian gastrula. 两栖动物原肠细胞外物质及神经外胚层的测定。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163463

S S Sánchez, F D Barbieri

引用次数: 5

Interaction between cisplatin-treated macrophages and Dalton's lymphoma cells in vitro. 顺铂处理巨噬细胞与体外道尔顿淋巴瘤细胞的相互作用。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163457

S M Singh, A Sodhi

引用次数: 14

Vesicle-mediated delivery of membrane to growth cones during neuritogenesis in embryonic rat primary neuronal cultures. 胚胎大鼠原代神经元培养过程中囊泡介导的膜向生长锥的传递。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163464

T B Shea, V S Sapirstein

{"title":"Vesicle-mediated delivery of membrane to growth cones during neuritogenesis in embryonic rat primary neuronal cultures.","authors":"T B Shea, V S Sapirstein","doi":"10.1159/000163464","DOIUrl":"https://doi.org/10.1159/000163464","url":null,"abstract":"Single cell suspensions from 15-day embryonic rat hindbrain plated on collagen formed large clumps by day 1 in culture. Neurite outgrowth was visible within 2 days. By day 14, morphological synapses were observed in nearly all instances of contact of a neurite ending with another cell. At day 3 in culture, the Golgi apparatus consisted of relatively few, broad lamellae. By contrast, at day 7 in culture this organelle consisted of tightly packed lamellar stacks with a considerable increase in vesicles budding from lamellae. Electron-lucent vesicles, ranging in size from 60 to 180 nm, similar to those generated by the Golgi apparatus were noted in neurite shafts and growth cones, with fusion of these vesicles virtually exclusively at the growth cone leading edge. Monensin resulted in the loss of these vesicles in cell somata and neuritic profiles. The electron-dense marker horseradish peroxidase was not incorporated into these vesicles following its addition to the culture medium, indicating that the vesicles were exocytotic. The number of total vesicles increased during the first 7 days of neurite outgrowth with no further increase up to day 14. This increase was due entirely to vesicles not labeled with the impermeable electron-dense stain ruthenium red, indicating that this increase represents actual vesicular elements and not increased surface convolutions. These data suggest that the 60- to 180-nm electron lucent vesicles are derived from the Golgi apparatus and, by fusion with the growth cone plasmalemma, provide new membrane required for neuritic outgrowth and maintenance.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 1-2","pages":"67-73"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163464","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14311731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Characterization of transformed cell lines evolving from a normal C3H line. 从正常C3H细胞系进化而来的转化细胞系的特性。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163459

L Curatolo, G Frascotti, P Ubezio, C Mannironi, L Morasca

引用次数: 1

Synergistic effect of natural human tumor necrosis factors alpha and beta in the clonogenic assay. 天然人肿瘤坏死因子α和β在克隆生成试验中的协同作用。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163495

M Miyake, S Fuchimoto, K Orita

引用次数: 2

Differential distribution of B16F10 melanoma cells in the liver lobule. B16F10黑色素瘤细胞在肝小叶中的差异分布。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163496

E Hilario, F Unda, S F Aliño

{"title":"Differential distribution of B16F10 melanoma cells in the liver lobule.","authors":"E Hilario, F Unda, S F Aliño","doi":"10.1159/000163496","DOIUrl":"https://doi.org/10.1159/000163496","url":null,"abstract":"The distribution pattern and the number of tumor cells arrested in the liver were studied in mouse livers. Mice were perfused intravascularly with a suspension of B16F10 melanoma cells. The animals were sacrificed at 0, 1, 5, and 20 min after tumor cell perfusion. The pattern of tumor cell distribution was studied by morphological methods, and by a combined method of fluorescent-tumor cell labelling and histochemical succinate dehydrogenase activity on frozen sections, in order to define the localization of tumor cells arrested in the liver lobule. The results show that the tumor cells have an exclusive distribution in the periportal regions of the liver lobule (identified as the high succinate dehydrogenase activity areas), and that the cells are not arrested in the pericentral regions (identified as the low succinate dehydrogenase activity areas). In addition, indomethacin treatment (2 mg/kg/day) induced an increase in the number of melanoma cells arrested in the liver, but a different distribution with respect to controls was not observed. These results show that periportal regions of the liver lobule constitute a particular domain in which the B16F10 melanoma cells present a special retention ability that can be modulated by indomethacin treatment.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 6","pages":"303-10"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163496","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14354787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Alterations in lactate dehydrogenase isoenzymes and phosphofructokinase activity in the hormonally induced G-XII cell line. 激素诱导的G-XII细胞系乳酸脱氢酶同工酶和磷酸果糖激酶活性的改变。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163489

A K Malik, N Sharma, P Chandiok, T N Chapekar

引用次数: 0

Mouse peritoneal macrophages produce CFU-gm and CFU-meg growth-promoting factors. 小鼠腹腔巨噬细胞产生CFU-gm和CFU-meg生长促进因子。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163460

M Sugimoto, Y Wakabayashi, S Hirose, M J Murphy

引用次数: 0

Ultrastructural and autoradiographic investigations of cell cultures derived from tendons or ligamentous material from patients with fibromatous disorders. 纤维瘤性疾病患者肌腱或韧带材料细胞培养的超微结构和放射自显影研究。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163469

J Neumüller, M Tohidast-Akrad, K Ammer, A Hakimzadeh, G Stransky, S Weis, G Partsch, R Eberl

{"title":"Ultrastructural and autoradiographic investigations of cell cultures derived from tendons or ligamentous material from patients with fibromatous disorders.","authors":"J Neumüller, M Tohidast-Akrad, K Ammer, A Hakimzadeh, G Stransky, S Weis, G Partsch, R Eberl","doi":"10.1159/000163469","DOIUrl":"https://doi.org/10.1159/000163469","url":null,"abstract":"Cell cultures were derived from tendons or ligamentous material from patients with carpal tunnel syndrome (CTS), Dupuytren's contracture (DP), tendopathia nodosa (TN) and hallux valgus (HV). The ultrastructure of the operation specimens as well as of the cell monolayers was investigated, using a floating sheet method in order to preserve both cell-to-cell contacts and the orientation of the monolayers. The histologic features of the tissues obtained in the operations were correlated with the ultrastructure of the cells in culture derived from these specimens. In DP, above all in the nodules, an activation of the capillary endothelium in the vicinity of myofibroblasts and mast cells was observed. In CTS the collagen fibrils varied extremely in diameter. In DP and TN biopsies a splicing process of helicoidly arranged fibrils could be seen. A disintegration of elastic fibers in the fibrillar and amorphous components was found in DP nodules, HV and TN tissues. Transitional forms between fibroblasts and myofibroblasts were observed not only in DP but also-though in a smaller percentage--in the cultures derived from the other patients. The cells showed organelles for active protein synthesis and transport. Autophagocytosis and the formation of multilamellated bodies took place in TN and HV cultures. In CTS, DP and TN cultures cells were connected via gap junctions. In some cultures, above all in those derived from CTS, monocilia were found. In CTS cultures the formation of intracellular collagen occurred. Growth parameters were rather low in HV cultures. PLmax (maximal pulse labelling index) values were higher in TN cultures than in DP and HV cultures. Plating efficiency (PE) values were higher in cultures derived from cell-rich and capillarized tissues than in biopsies with few cells.","PeriodicalId":75839,"journal":{"name":"Experimental cell biology","volume":"56 3","pages":"113-30"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000163469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14359437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Correlation of anionic residue mobility at the filopodial plasma membranes with multilayer formation in transformed 3T3 cells. 转化3T3细胞中丝状质膜阴离子残基迁移率与多层膜形成的关系。

Experimental cell biology Pub Date : 1988-01-01 DOI: 10.1159/000163472

N Kawai, M Kawai, T Nishimura, K Arimura, I Ichihara

引用次数: 1