Cell differentiation最新文献_第6页

Somitogenesis in the mouse embryo 小鼠胚胎中的体细胞发生

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90033-4

David Ostrovsky , Joseph W. Sanger , James W. Lash

引用次数: 27

Factors involved in the formation and stabilization of cell aggregates obtained from amphibian embryonic explants 两栖动物胚胎外植体中细胞聚集体形成和稳定的相关因素

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90038-3

Mats-Olof Mattsson, Huguette Løvtrup-Rein, Søren Løvtrup

引用次数: 2

The jellyfish and its polyp: a comparative study of gene expression monitored by the protein patterns, using two-dimensional gels with double-label autoradiography 水母和它的息肉:通过蛋白质模式监测基因表达的比较研究，使用二维凝胶和双标签放射自显影

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90041-3

Andreas Bally, Volker Schmid

{"title":"The jellyfish and its polyp: a comparative study of gene expression monitored by the protein patterns, using two-dimensional gels with double-label autoradiography","authors":"Andreas Bally, Volker Schmid","doi":"10.1016/0045-6039(88)90041-3","DOIUrl":"10.1016/0045-6039(88)90041-3","url":null,"abstract":"<div><p>The life cycle of <em>Podocoryne carnea</em> (Coelenterata, Anthomedusae) shows several distinct stages which differ considerably in terms of their ecology, morphology, cellular composition, and ultrastructure. Previously these stages had even been described as separate species. Using two-dimensional gel electrophoresis and a new method of double-label autoradiography, we show here for the first time for metagenic hydrozoans that only minor differences in gene expression exist between the various life cycle stages. Our results demonstrate the high resolution power of these techniques and show that the different life stages of <em>P. carnea</em> remain rather similar on the protein level. Most of the prominent spots of the two-dimensional gel protein patterns are common to all stages studied. These data show that the hydrozoan life cycle and development are regulated by only minor distinctions in gene expression which possibly explains the great morphogenetic repertoire of these animals described in many studies.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 1","pages":"Pages 93-102"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90041-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14031996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Lack of differentiation following mouse serum treatment of cultured chick limb myoblasts 鸡肢体成肌细胞经小鼠血清处理后缺乏分化

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90042-5

Rita B. Finley , Curtis L. Parker

{"title":"Lack of differentiation following mouse serum treatment of cultured chick limb myoblasts","authors":"Rita B. Finley , Curtis L. Parker","doi":"10.1016/0045-6039(88)90042-5","DOIUrl":"10.1016/0045-6039(88)90042-5","url":null,"abstract":"<div><p>This investigation was conducted to assess the effects of mouse serum on chick skeletal muscle cell differentiation. In light of earlier findings of altered membrane phospholipid metabolism following mouse serum treatment of Friend erythroleukemic and chick chondrogenic cells, it was of interest to determine whether similar changes would modulate the fusion of mononucleated myoblasts, which is necessary for the formation of multinucleated skeletal muscle fibers. When mouse serum is added to low density cultures of enriched chick myoblasts shortly following cell attachment to the substratum, fusion is inhibited and neutral lipid accumulation ensues. There is an early inhibitory effect on DNA synthesis but not on protein synthesis. There is no increase in the uptake of 2-deoxyglucose following insulin stimulation of the cells, which suggests that while the cells are accumulating large amounts of lipid, they are not being converted into typical adipocytes. Finally, even in cultures of mouse serum-treated cells that undergo significant fusion, one observes thinner myotubes that do not spontaneously contract as do those of control cultures, as well as a disorganization of fluorescently stained actin and myosin myofilaments. These findings demonstrate that mouse serum acts in a dose-dependent manner, is not cytotoxic to the cells, but is capable of modulating normal developmental events of myoblasts as reported for other cell and tissue types.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 1","pages":"Pages 103-117"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90042-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13600927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Stage-specific gene expression in erythroid progenitor cells (CFU-E) 红细胞祖细胞(CFU-E)分期特异性基因表达

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90017-6

Yuji Mishina, Yasuhisa Matsui, Masuo Obinata

{"title":"Stage-specific gene expression in erythroid progenitor cells (CFU-E)","authors":"Yuji Mishina, Yasuhisa Matsui, Masuo Obinata","doi":"10.1016/0045-6039(88)90017-6","DOIUrl":"10.1016/0045-6039(88)90017-6","url":null,"abstract":"<div><p>In erythropoietic differentiation, mature red blood cells are generated from specific progenitor cells through the action of specific growth regulatory molecules. To know the mechanism of differentiation, it is important to examine the control of gene expression in these progenitor cells in combination with growth regulatory molecules. We have cloned two genes expressing at a maximal level in the CFU-E (colony forming unit-erythroid), one of the erythroid progenitor cells from novel murine erythroleukemia (MEL) cell line (TSA8) which can be induced to CFU-E in vitro. The expression of these genes is well correlated with the appearance of CFU-E during induction of TSA8 cells, and is higher in the CFU-E-cells enriched from mouse fetal livers than in the more differentiated erythroid cells. Combining these with our previous results, it is suggested that in the erythropoiesis the progenitor cells have distinct patterns of gene expression. This expression is replaced through each progenitor cell rather than by the continuous increase in the expression of a set of genes specific to the mature erythroid cell following the commitment process.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 3","pages":"Pages 259-265"},"PeriodicalIF":0.0,"publicationDate":"1988-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90017-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14292769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Retina cognin does not bind to itself during membrane interaction in vitro 视网膜认知素在体外膜相互作用中不与自身结合

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90014-0

N.M. Troccoli, R.E. Hausman

{"title":"Retina cognin does not bind to itself during membrane interaction in vitro","authors":"N.M. Troccoli, R.E. Hausman","doi":"10.1016/0045-6039(88)90014-0","DOIUrl":"10.1016/0045-6039(88)90014-0","url":null,"abstract":"<div><p>Retina cognin (R-cognin) is an intrinsic membrane protein of vertebrate retinal cells which supports tissue-specific cell adhesion and mediates cell type-specific associations during development. As a first step in understanding how R-cognin mediates specific adhesion of retinal cell membranes, we asked if cognin bound to another cognin molecule or to a different macromolecule, a possible cognin-binding protein. To do this, we constructed an affinity column with retinal cell membrane proteins (enriched for cognin) bound to the matrix. Proteins in a detergent extract of retinal cell membranes were exposed to this matrix and those which bound specifically eluted and identified by immunoelectrophoresis. Most prominent among these was a protein with an apparent mass of 64 kDa. The binding of this material to the column was blocked by cognin antibody. To eliminate possible artifacts of molecular interactions in vitro, we sought independent confirmation that 64 kDa protein actually bound R-cognin. Using a modified retina membrane vesicle system, we asked what proteins could be photoaffinity cross-linked to cognin during vesicle aggregation. Cross-linking produced a 114 kDa complex on gels which could be resolved into a 50 kDa (cognin) and a 64 kDa band under reducing conditions. Identification of a 64 kDa protein by independent techniques suggests that cognin promotes association of embryonic chick neural retina cells by binding to this macromolecule or these molecules. Identification of a second component in the mechanism should allow elucidation of cognin's role in mediating cell-cell interactions in developing neural retina.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 3","pages":"Pages 225-231"},"PeriodicalIF":0.0,"publicationDate":"1988-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90014-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14482876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Contents Volume 22 目录第22卷

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90018-8

引用次数: 0

S-Adenosylmethionine, S-adenosylhomocysteine and S-adenosylhomocysteine hydrolase variations during differentiation of Dictyostelium discoideum s -腺苷蛋氨酸、s -腺苷同型半胱氨酸和s -腺苷同型半胱氨酸水解酶在盘状盘基钢的分化过程中的变化

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90012-7

M.C. Guitton , B.T. Keller , D. Part , J. De Gunzburg , R.T. Borchardt , M. Véron

引用次数: 7

Organization of lymphocyte plasma membrane. Surface protein-membrane matrix interactions in B-cell lines of different stages of differentiation 淋巴细胞质膜组织。不同分化阶段b细胞系表面蛋白与膜基质的相互作用

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90015-2

Guido Trenn , Hajime Takayama , Wendy F. Davidson , Herbert C. Morse III , Michail V. Sitkovsky

{"title":"Organization of lymphocyte plasma membrane. Surface protein-membrane matrix interactions in B-cell lines of different stages of differentiation","authors":"Guido Trenn , Hajime Takayama , Wendy F. Davidson , Herbert C. Morse III , Michail V. Sitkovsky","doi":"10.1016/0045-6039(88)90015-2","DOIUrl":"10.1016/0045-6039(88)90015-2","url":null,"abstract":"<div><p>Composition of surface proteins and their interactions with cytoskeleton or membrane matrix were compared in tumor B-cell lines of different stages of B-lymphocyte maturation. All studied B-cell lines were found to share a similar set of cell surface proteins, which are tightly associated with the cytoskeleton. The increase in amount of detergent-unextractable cell surface proteins with B-cell maturation suggested that differentiation of B lymphocytes was accompanied by development of specific interactions between surface proteins and elements of the cytoskeleton or membrane matrix. Using a recently developed procedure for lymphocyte plasma membrane fractionation we demonstrate changes in distribution of cell surface proteins in membrane matrix-rich and membrane matrix-poor plasma membrane fractions during B-lymphocyte maturation. Thus, cell surface proteins of the mature B-cell line MOPC-315 were predominantly found in the plasma membrane vesicles of a high buoyant density. These vesicles mostly contained plasma membrane proteins tightly associated with elements of the membrane matrix. In immature B cells (line 70Z3) virtually all surface proteins were detected in both low and high buoyant density membrane vesicles. The tendency to increased associations between surface proteins and cytoskeleton/membrane matrix with maturation of B cells could not be explained by increased amounts of filamentous actin, since no correlation was found between the amount of globular or filamentous actin and the degree of surface protein-cytoskeleton (membrane matrix) interactions.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 3","pages":"Pages 233-244"},"PeriodicalIF":0.0,"publicationDate":"1988-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90015-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14386261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The potential for transdifferentiation and regeneration of isolated striated muscle of medusae in vitro 水母离体横纹肌体外转分化和再生的潜力

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90009-7

Volker Schmid

引用次数: 17