{"title":"A monoclonal antibody recognizing a common antigen on neurons and fibroblasts in chicken and quail","authors":"Monique Graniou, Marcelle Chanconie, Catherine Ziller","doi":"10.1016/0045-6039(88)90049-8","DOIUrl":"10.1016/0045-6039(88)90049-8","url":null,"abstract":"<div><p>A monoclonal antibody, FiN1, obtained by immunization of a mouse with homogenates of embryonic quail nodose ganglia, was found to react with a surface antigenic determinant, both in quail and chick, present on practically all neurons of the spinal cord and of the peripheral nervous system and on a subpopulation of fibroblasts. An ontogenetic study performed on tissue sections, cell suspensions and cultures showed that FiN1 defines a differentiation marker which appears relatively late in development, during the second half of embryonic life, and persists after hatching. The onset and evolution of its expression during development varies in a tissue-specific manner.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 3","pages":"Pages 179-190"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90049-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14335619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cytoskeleton in preimplantation mouse development","authors":"E. Lehtonen, G. Ordo´nez, I. Reima","doi":"10.1016/0045-6039(88)90048-6","DOIUrl":"10.1016/0045-6039(88)90048-6","url":null,"abstract":"<div><p>This paper reviews the constituents of the cytoskeleton in the cells of the preimplantation mouse embryo and how they change as the development proceeds. The cytoskeleton can be divided into two distinct groups, that in the cytosplasm and that associated with the membrane. The first and better-known group contains microfilaments, microtubules and intermediate filaments, the second such components of the cell and nuclear membrane as spectrin-like protein and nuclear lamin. The filamentous components of the cytoplasmic cytoskeleton adhere to the nuclear and cell membrane at attachment points where specific proteins such as vinculin may mediate the interaction. Each cell of the early embryo has all of these components, but their morphological organization and molecular constitution alter as the embryo develops. These modifications are especially pronounced when the cleavage-stage embryo compacts and when the blastocyst forms and differentiates. These events represent the most critical stages of morphogenesis and cytodifferentiation in the preimplantation embryo. The cytoskeleton may thus have an important role in the control of the early mammalian development.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 3","pages":"Pages 165-177"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90048-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14194237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Glomerular sialoconjugates of developing and mature rat kidneys","authors":"H. Holtho¨fer , R.A. Hennigar , B.A. Schulte","doi":"10.1016/0045-6039(88)90053-X","DOIUrl":"10.1016/0045-6039(88)90053-X","url":null,"abstract":"<div><p>The appearance of sialoconjugates in developing rat kidney glomeruli was studied using lectins and neuraminidase-lectin staining sequences. In the early S-shaped bodies, binding of<em>Maclura pomifera</em> (MPA; specific for galactosaminyl residues of glycoconjugates) could be detected in the presumptive podocyte layer at the apex of these cells, but notably no binding of lectins specific for sialic acid could be seen. During further morphologic maturatin of the S-shaped bodies, binding of<em>Limax flavus</em> (LFA; specific for sialic acids) and<em>Triticum vulgaris</em> (WGA; specific for sialic acids and <em>N</em>-acetyl glucosaminyl moieties) appeared at the apex of podocytes and extended subsequently along the lateral membranes to the base of these cells. In morphologically mature glomeruli, LFA stained not only the base of podocytes but also glomerular basement membranes. WGA and MPA bound to the capillary endothelia as well as to the structures bound by LFA. The intensity of WGA binding increased considerably after 5 days of postnatal life, seemingly in parallel with the decrease and ultimate disappearance of MPA binding. In addition to showing individual appearance pattern for various lectin binding sites, these studies give evidence of previously unrecognized postnatal completion of the components of glomerular filtration barrier.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 3","pages":"Pages 215-221"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90053-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14337271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Presence of serotonin in early chick embryos","authors":"Hadar Emanuelsson , Mats Carlberg , Bertil Lo¨wkvist","doi":"10.1016/0045-6039(88)90050-4","DOIUrl":"10.1016/0045-6039(88)90050-4","url":null,"abstract":"<div><p>With biochemical analysis and with autoradiography based on injection of 5-[<sup>3</sup>H]hydroxytryptophan, it was possible to demonstrate the presence of serotonin (5-hydroxytryptamine) in early chick embryos as early as the pre-steak stage. The biochemical analysis which covered the early developmental period (0.5–6 days of incubation) revealed an elevated concentration of serotonin at gastrulation; from then it stayed at a lower and fairly even level. Autoradiographs of embryos at the pre-streak stage, the primitive streak stage, the head fold stage and the 4–6 somites stage indicated the presence of serotonin in intracellular yolk granules and in cell nuclei. Moreover, the amine appeared associated with microfilaments and microtubules, particularly in developing neural cells. Notably the elevated concentration of serotonin at gastrulation, but also the intracellular distribution of the amine during early organogenesis, indicates a prominent role for it in cell-shape changes and morphogenesis in the early chick embryo.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 3","pages":"Pages 191-199"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90050-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14393059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Differential expression of the laminin A and B chains in chimeric kidneys","authors":"K. Holm , L. Risteli , H. Sariola","doi":"10.1016/0045-6039(88)90054-1","DOIUrl":"10.1016/0045-6039(88)90054-1","url":null,"abstract":"<div><p>The expression of laminin in embryonic kidneys growing in ovo is followed with mouse-specific, affinity-purified antibodies against the laminin A and B chains. In mouse kidneys growing on the chicken chorioallantoic membrane, the epithelium and nephrogenic mesenchyme are derived from mouse and the vasculature from chicken chorioallantoic vessels. Hence, with the mouse-specific antibodies, it is possible to analyze the deposition of laminin chains by the nephrogenic tissue, because laminin derived from the chicken vasculature remains unstained. In these chimeras, only the laminin B chain, but not the A chain, is expressed in the undifferentiated nephrogenic mesenchyme. The basement membrane around the ureter bud is labeled by the antibodies against both laminin A and B chains. In the mesenchyme, the laminin A chain appears when the mesenchyme converts into tubules. The results suggest that the laminin A and B chains are synthesized differentially in the embryonic nephrogenic tissue.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 3","pages":"Pages 223-227"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90054-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14337272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M.C. Roccheri , M. La Rosa , M.G. Ferraro , M. Cantone , D. Cascino , G. Giudice , G. Sconzo
{"title":"Stress proteins by zinc ions in sea urchin embryos","authors":"M.C. Roccheri , M. La Rosa , M.G. Ferraro , M. Cantone , D. Cascino , G. Giudice , G. Sconzo","doi":"10.1016/0045-6039(88)90052-8","DOIUrl":"10.1016/0045-6039(88)90052-8","url":null,"abstract":"<div><p>In<em>Paracentrotus lividus</em> embryos, treatment with zinc ions induces the synthesis of the two major stress proteins with the same molecular weight as those induced by heat shock. The developmental stages responsive to zinc ion treatment are the same as those responsive to heat shock. However, zinc treatment induces a longer lasting synthesis of the stress proteins, and, unlike heat shock, does not induce thermotolerance and does not inhibit synthesis of the bulk proteins.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 3","pages":"Pages 209-213"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90052-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14337270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cell differentiation as assayed by the topography and number of ribosomal genes","authors":"V.Y. Brodsky , T.L. Marshak , A.A. Karavanov , O.V. Zatsepina , V.V. Nosikov , L.I. Korochkin , E.A. Braga","doi":"10.1016/0045-6039(88)90051-6","DOIUrl":"10.1016/0045-6039(88)90051-6","url":null,"abstract":"<div><p>In situ hybridization, Ag-staining and electron microscopy were used to study the distribution of ribosomal genes in isolated nuclei of rat cerebellar cells and the correspondence of the ribosomal genome topography to the nucleolar structure. rDNA-DNA autoradiography revealed clusters of silver grains, as well as diffuse groups and rows. The cluster frequencies corresponded to the frequencies of nucleoli on Ag-stained slides. Competitive hybridization in situ using unlabelled rat rRNA and hybridization with a nonspacer rDNA fragment showed that the diffuse groups and rows of grains also correspond to the ribosomal genes. Spatial organization of the ribosomal genome in the Purkinje cells differs from that in the other cerebellar neurons and glial cells. A 1.5-fold redundancy of the ribosomal genes was found in some Purkinje cells, while most of these as well as microneurons contained the diploid value of the genes.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 3","pages":"Pages 201-207"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90051-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14335620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}