Cell differentiation最新文献_第2页

Isolation and characterization of a sea urchin hsp 70 gene segment 海胆hsp 70基因片段的分离与鉴定

Cell differentiation Pub Date : 1988-07-01 DOI: 10.1016/0045-6039(88)90061-9

G. Sconzo , M. La Rosa , M. La Farina , M.C. Roccheri , D. Oliva , G. Giudice

引用次数: 6

Membrane-induced segregation of motile and stable domains in cytoplasm: possible role in morphological differentiation of tissue cells 细胞膜诱导细胞质中运动和稳定结构域的分离:在组织细胞形态分化中的可能作用

Cell differentiation Pub Date : 1988-07-01 DOI: 10.1016/0045-6039(88)90059-0

J.M. Vasiliev, I.M. Gelfand

引用次数: 5

Corticosterone effects on differentiation and X-ray-induced transformation of C3H/10T1/2 mouse cells 皮质酮对小鼠C3H/10T1/2细胞分化和x射线诱导转化的影响

Cell differentiation Pub Date : 1988-07-01 DOI: 10.1016/0045-6039(88)90067-X

Duane L. Guernsey, Thomas J. Schmidt

引用次数: 5

Embryonic mouse lung morphogenesis and type II cytodifferentiation in serumless, chemically defined medium using prolonged in vitro cultures 胚胎小鼠肺形态发生和II型细胞分化在无血清，化学定义的培养基中使用长时间体外培养

Cell differentiation Pub Date : 1988-07-01 DOI: 10.1016/0045-6039(88)90062-0

Tina F. Jaskoll, Grace Don-Wheeler, Randall Johnson, Harold C. Slavkin

{"title":"Embryonic mouse lung morphogenesis and type II cytodifferentiation in serumless, chemically defined medium using prolonged in vitro cultures","authors":"Tina F. Jaskoll, Grace Don-Wheeler, Randall Johnson, Harold C. Slavkin","doi":"10.1016/0045-6039(88)90062-0","DOIUrl":"10.1016/0045-6039(88)90062-0","url":null,"abstract":"<div><p>The timing, position and mechanism(s) for determining type II cytodifferentiation during mammalian lung development are not known. To approach this problem, we have cultured Theiler stage 16 embryonic B10.A strain mouse lung primordia (12-days gestation, E12) in serumless, chemically defined medium in the presence or absence of dexamethasone (DEX) for periods up to 27 days in vitro. Morphogenesis and cytodifferentiation were evaluated by light and transmission electron microscopy, and immunochemical techniques. Pulmonary surfactant-associated apoproteins (PSAP) were initially expressed by type II cells at 16.5-day gestation in vivo. DEX-supplementation to the culture medium resulted in the accelerated expression of PSAP; the apoprotein isoforms (A<sub>1</sub>, A<sub>2</sub>, and A<sub>3</sub>) produced in vitro were comparable to those synthesized during fetal and postnatal in vivo development by high resolution, two-dimensional gel electrophoresis coupled with immunoblot staining. Cultures without DEX produced PSAP A<sub>2</sub> and A<sub>3</sub> isoforms, but did not produce A<sub>1</sub> (26–31 kDa, p<em>I</em> 5.2–5.3). DEX-treated cultures produced more lamellar bodies within type II cells than non-treated controls. The results demonstrate that long-term cultures of embryonic lung primordia express morphogenesis, cytodifferentiation and the synthesis and secretion of PSAP in the absence of exogenous hormones or growth factors. The data set further supports the hypothesis that morphogenesis and type II cytodifferentiation are regulated by autocrine and paracrine factors intrinsic to the embryonic lung developmental program and independent of exogenous hormone controls.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 2","pages":"Pages 105-117"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90062-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14193519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Spatial and temporal changes in the pattern of glycosylation of the developing chick limb tissue components as revealed by fluorescent conjugated lectin probes 荧光偶联凝集素探针显示发育中的鸡肢体组织组分糖基化模式的时空变化

Cell differentiation Pub Date : 1988-07-01 DOI: 10.1016/0045-6039(88)90066-8

J.M. Hurle , M.A. Ros , J.R. Hinchliffe

{"title":"Spatial and temporal changes in the pattern of glycosylation of the developing chick limb tissue components as revealed by fluorescent conjugated lectin probes","authors":"J.M. Hurle , M.A. Ros , J.R. Hinchliffe","doi":"10.1016/0045-6039(88)90066-8","DOIUrl":"10.1016/0045-6039(88)90066-8","url":null,"abstract":"<div><p>The changing pattern of expression of glycoconjugates during the differentiation of the chick leg bud between stages 17 to 34 (days 3 to 8 of incubation) was studied using fluorochrome-labelled plant lectins. Limb buds were fixed in cold acetic-alcohol and wax-embedded. Agglutinins of peanut (PNA), soybean (SBA) and succinylated wheat germ (WGAs) revealed a specific binding pattern in the apical ectodermal ridge (AER) between Hamburger and Hamilton stages 19–32. These stages coincide with the period of elevation of the AER. This specific binding pattern was absent from the adjacent dorsal and ventral ectoderm. Prechondrogenic cells were positive for WGA and for PNA, and the PNA-binding capacity was intensified after neuraminidase treatment. Premyogenic cells at stage 23 can be identified as negative to PNA after neuraminidase, while the blood vessels became positive. PNA, SBA, WGA, WGAs and, in addition, <em>Ricinus communis</em> (RCA-I) lectins stained the basal membrane. Strands of extracellular matrix which connect with the basal membrane and cross the limb transversely between dorsal and ventral ectoderm were stained by RCA-I, SBA and PNA after neuraminidase.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 2","pages":"Pages 149-158"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90066-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14335615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Expression of brush border calmodulin-binding proteins during human small and large bowel differentiation 刷缘钙调蛋白结合蛋白在人小肠和大肠分化过程中的表达

Cell differentiation Pub Date : 1988-07-01 DOI: 10.1016/0045-6039(88)90063-2

C. Rochette-Egly, B. Lacroix, K. Haffen, M. Kedinger

{"title":"Expression of brush border calmodulin-binding proteins during human small and large bowel differentiation","authors":"C. Rochette-Egly, B. Lacroix, K. Haffen, M. Kedinger","doi":"10.1016/0045-6039(88)90063-2","DOIUrl":"10.1016/0045-6039(88)90063-2","url":null,"abstract":"<div><p>The expression and immunocytochemical localization of three brush border cytoskeletal calmodulin-binding proteins, caldesmon, fodrin, and the 110 kDa subunit of the 110 kDa calmodulin complex, have been studied in human intestinal epithelial cells as a function of their ontogenic differentiation. At immature stages (fetal week 8), caldesmon and fodrin were present in undifferentiated intestinal epithelial cells. However, no 110 kDa protein was detectable except a 135 kDa immunoreactive species. The 110 kDa form appeared at week 12, when microvilli differentiate, and became prominent at week 14 simultaneously with the disappearance of the 135 kDa species. Finally at week 14, the calmodulin-binding protein pattern was identical to that found in adults. Immunocytochemical experiments revealed that at week 8, antibodies to caldesmon and fodrin gave a fluorescence lining at the periphery of the cells, whereas the 110 kDa immunoreactive species was hardly detectable. Then, as early as week 12 of gestation, with the three antisera, a bright fluorescence lined the apex of the cells, as in adults. In the colon, the events were delayed. This study demonstrates that the developmental pattern of the three calmodulin-binding proteins investigated, caldesmon, fodrin and the 110 kDa subunit, parallels the temporal differentiation of human intestinal brush borders and the proximo-distal morphological intestinal maturation.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 2","pages":"Pages 119-131"},"PeriodicalIF":0.0,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90063-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14193520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Lectin activity and distribution of chicken lactose lectin I in the extracellular matrix of the chick developing kidney 鸡乳糖凝集素I在发育中的鸡肾细胞外基质中的活性和分布

Cell differentiation Pub Date : 1988-07-01 DOI: 10.1016/0045-6039(88)90060-7

Eliane Didier , Pierre Didier , Danielle Bayle , Michelle Chevalier

引用次数: 13

Exogenous δ-crystallin gene expression as probe for differentiation of teratocarcinoma stem cells 外源性δ-晶体蛋白基因表达对畸胎瘤干细胞分化的影响

Cell differentiation Pub Date : 1988-07-01 DOI: 10.1016/0045-6039(88)90065-6

Koji Goto, Shigeo Hayashi, Yasuaki Shirayoshi, Masatoshi Takeichi, Hisato Kondoh

引用次数: 22

Analysis of cytoplasmic factors in developmental cleavage of mouse embryo 小鼠胚胎发育分裂的细胞质因素分析

Cell differentiation Pub Date : 1988-07-01 DOI: 10.1016/0045-6039(88)90064-4

S. Suzuki , S. Komatsu , H. Kitai , Y. Endo , R. Iizuka , T. Fukasawa