Koji Goto, Shigeo Hayashi, Yasuaki Shirayoshi, Masatoshi Takeichi, Hisato Kondoh
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Exogenous δ-crystallin gene expression as probe for differentiation of teratocarcinoma stem cells
We developed an experimental system in which differentiation of teratocarcinoma stem cell is probed by expression of stably introduced exogenous genes. We used chicken δ-crystallin gene (δ gene) and its derivative (Moδ gene) driven by long terminal repeat (LTR) promoter of Moloney murine leukemia virus (Mo-MuLV). Neither of the genes was expressed in the undifferentiated condition. Differentiation to primitive endoderm induced by retinoic acid (RA) led to expression of δ but not Moδ, while differentiation to more advanced endodermal cells by RA plus dibutyryl cAMP elicited Moδ expression in addition to δ. These results are interpreted as a consequence of differential activation/suppression of gene expression through enhancer elements associated with the genes.
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