Cell differentiation最新文献_第10页

Developmental strategies of the angiosperm pollen: a biochemical perspective 被子植物花粉的发育策略:生化视角

Cell differentiation Pub Date : 1987-09-01 DOI: 10.1016/0045-6039(87)90476-3

V. Raghavan

引用次数: 25

Fertilization induces endocytosis in Xenopus eggs 受精诱导爪蟾卵内吞作用

Cell differentiation Pub Date : 1987-09-01 DOI: 10.1016/0045-6039(87)90480-5

Giovanni Bernardini, Marco Ferraguti, Roberto Stipani

引用次数: 9

Subject index volume 21 主题索引第21卷

Cell differentiation Pub Date : 1987-09-01 DOI: 10.1016/0045-6039(87)90485-4

引用次数: 0

Proteoglycan biosynthesis in relation to differentiation of cord blood monocytes in vitro 蛋白多糖生物合成与脐带血单核细胞体外分化的关系

Cell differentiation Pub Date : 1987-08-01 DOI: 10.1016/0045-6039(87)90455-6

L. Uhlin-Hansen , S.O. Kolset

{"title":"Proteoglycan biosynthesis in relation to differentiation of cord blood monocytes in vitro","authors":"L. Uhlin-Hansen , S.O. Kolset","doi":"10.1016/0045-6039(87)90455-6","DOIUrl":"10.1016/0045-6039(87)90455-6","url":null,"abstract":"<div>Human monocytes were obtained from umbilical cord blood and cultured in vitro. By morphological criteria, the neonatal monocytes developed into macrophage-like cells in the course of 3–5 days in culture. The cells were exposed to [35S]sulphate for 24 h, either from day 0–1 or day 9–10 in vitro. The 35S-labelled macromolecules recovered were mainly associated with the medium fraction (approximately 75%) in both day 1 and day 10 cultures. These secretory macromolecules were demonstrated by the use of chondroitinase ABC-digestions to contain predominately chondroitin sulphate proteoglycan (CSPG). [35S]galactosaminoglycan chains from day 10 cultures were more highly sulphated than the corresponding day 1 species due to the appearance of (glucuronosyl-4,6-diS-N-acetylgalactosamine) disulphated disaccharide units. The galactosaminoglycan chains in neonatal CSPG were found to increase in Mr during cultivation in vitro; from mean Mr of 20 400 to 30 200 (n = 5) in day 1 and day 10 medium proteoglycans, respectively. The corresponding Mr values for adult monocyte [35S]galactosaminoglycan chains were 21 300 and 22 800. On the basis of the concomitant changes in cellular morphology and glycosaminoglycan structure, it is concluded that neonatal monocytes, like monocytes from adults, differentiate into macrophage-like cells in vitro.</div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"21 3","pages":"Pages 189-197"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(87)90455-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14772515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Presence of a unique glycoconjugate on the surface of rat primordial germ cells during migration 大鼠原始生殖细胞在迁移过程中表面存在一种独特的糖缀合物

Cell differentiation Pub Date : 1987-08-01 DOI: 10.1016/0045-6039(87)90456-8

A.R. Fazel , B.A. Schulte , R.P. Thompson , S.S. Spicer

引用次数: 48

An immunofluorescence study of chondrogenesis in murine mandibular ectomesenchyme 小鼠下颌骨外切间充质软骨形成的免疫荧光研究

Cell differentiation Pub Date : 1987-08-01 DOI: 10.1016/0045-6039(87)90453-2

Joy M. Richman, Virginia M. Diewert

引用次数: 8

Somite chondrogenesis: the role of the microenvironment some some软骨形成:微环境的作用

Cell differentiation Pub Date : 1987-08-01 DOI: 10.1016/0045-6039(87)90452-0

Nagaswami Sri Vasan

引用次数: 18

MB1, a quail leukocyte/vascular endothelium antigen: characterization of the lymphocyte-surface form and identification of its secreted counterpart as α2-macroglobulin MB1，鹌鹑白细胞/血管内皮抗原:淋巴细胞表面形态的表征及其分泌对应物α2巨球蛋白的鉴定

Cell differentiation Pub Date : 1987-08-01 DOI: 10.1016/0045-6039(87)90454-4

Bruno Péault

{"title":"MB1, a quail leukocyte/vascular endothelium antigen: characterization of the lymphocyte-surface form and identification of its secreted counterpart as α2-macroglobulin","authors":"Bruno Péault","doi":"10.1016/0045-6039(87)90454-4","DOIUrl":"https://doi.org/10.1016/0045-6039(87)90454-4","url":null,"abstract":"<div>A monoclonal antibody (α-MB1) binds to a cell surface marker expressed throughout ontogeny and adult life by vascular endothelial and hemopoietic cells of the quail, with the exception of erythrocytes, although it was raised against the heavy chain of quail immunoglobulin M. In addition to an 80 kDa polypeptide accounting for immunoglobulin μ chain, α-MB1 stains intensely a 180-kDa band on Western blots of reduced plasma proteins. We have previously characterized MB1 antigens of quail endothelial cells as glycoproteins of apparent molecular masses ranging from 80 to 200 kDa and provided evidence for the participation of vascular endothelium in the secretion of α-MB1-positive plasma components. We demonstrate here that this circulating material is the proteinase inhibitor α2-macroglobulin. Furthermore, the MB1 antigens immunoprecipitated from lymphocytes are shown to be essentially similar to their endothelial counterparts, suggesting that the same molecular complex is expressed by all the elements of the hemangioblastic cell lineage. Finally, the cross reactivity of the α-MB1 antibody with immunoglobulin μ chain is confirmed and shown to occur via a carbohydrate epitope.</div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"21 3","pages":"Pages 175-187"},"PeriodicalIF":0.0,"publicationDate":"1987-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(87)90454-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92062199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Cross-linking of membrane glycoconjugates is not a sufficient condition for neural induction by concanavalin A 膜糖缀合物的交联不是豆豆蛋白a诱导神经的充分条件

Cell differentiation Pub Date : 1987-07-01 DOI: 10.1016/0045-6039(87)90416-7

Lydie Gualandris , Anne-Marie Duprat , Pierre Rougé

引用次数: 1

The epitope of mouse embryonic antigen(s) recognized by monoclonal antibody TEC-02 is a carbohydrate carried by high-molecular-weight glycoconjugates 单克隆抗体TEC-02识别的小鼠胚胎抗原表位是一个由高分子量糖缀合物携带的碳水化合物

Cell differentiation Pub Date : 1987-07-01 DOI: 10.1016/0045-6039(87)90419-2

Petr Dráber

{"title":"The epitope of mouse embryonic antigen(s) recognized by monoclonal antibody TEC-02 is a carbohydrate carried by high-molecular-weight glycoconjugates","authors":"Petr Dráber","doi":"10.1016/0045-6039(87)90419-2","DOIUrl":"10.1016/0045-6039(87)90419-2","url":null,"abstract":"<div>The expression and properties of mouse embryonic antigens, recognized by monoclonal antibody TEC-02, were analyzed in teratocarcinoma-derived cell lines. TEC-2 antigens were found in the majority of the parietal endoderm cells PYS-2 and in a fraction of cultured embryonal carcinoma cells but not in other cell lines tested. During the course of retinoic acid-induced differentiation of embryonal carcinoma cells F9, the expression of TEC-2 was transiently increased. Immunolabeling of extracts from F9 and PYS-2 cells separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that TEC-2 antigens are polydisperse glycoconjugates of high molecular weight (mostly > 100 000). The TEC-2 epitope was shown to be carbohydrate which in F9 cells might be attached to the same carrier as another developmentally regulated carbohydrate epitope TEC-1. The TEC-2 antigens, isolated by indirect immunoprecipitation, were degraded by extensive pronase digestion or mild alkaline treatment to mostly large products. Immunostaining of glycolipid standards suggested that TEC-2 epitope involves the GalNAcβ1 → 4Galβ1 → 4R sequence. Combined data indicate that TEC-2 is a new developmentally regulated carbohydrate epitope carried in embryonal carcinoma cells predominantly on glycoprotein-bound large carbohydrates.</div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"21 2","pages":"Pages 119-130"},"PeriodicalIF":0.0,"publicationDate":"1987-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(87)90419-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13589298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21