Cell differentiation最新文献_第5页

Changes in cytoplasmic pH are involved in the cell type regulation of Dictyostelium 细胞质pH值的变化参与盘基骨的细胞类型调节

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90044-9

Rob J. Aerts

引用次数: 18

Amplification of ribosomal genes and formation of extrachromosomal nucleoli in oocytes of starfish Henricia hayashi (Asteroidea: Echinasteridae) 海星卵母细胞核糖体基因扩增及染色体外核仁的形成(星总科:棘星科)

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90036-X

Elena R. Gaginskaya , Vladimir L. Kasyanov , Galina L. Kogan

引用次数: 6

The expression of melanosomal matrix protein in the transdifferentiation of pigmented epithelial cells into lens cells 色素上皮细胞向晶状体细胞转分化过程中黑素体基质蛋白的表达

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90045-0

Makoto Mochii, Takashi Takeuchi, Ryuji Kodama, Kiyokazu Agata, Goro Eguchi

引用次数: 43

Contents of development, growth & differentiation 发展、成长和分化的内容

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90047-4

引用次数: 0

Molecular cloning of cell-type-specific cDNAs exhibiting new types of developmental regulation in Dictyostelium discoideum 显示盘状盘齿龙发育调控新类型的细胞特异性cdna的分子克隆

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90043-7

Toshinori Ozaki , Miki Hasegawa , Yoshio Hamada , Masao Tasaka , Masaki Iwabuchi , Ikuo Takeuchi

引用次数: 15

Expression of the Ca2+ mobilizing muscarinic system in the chick embryo correlates with morphogenesis 鸡胚Ca2+动员毒蕈碱系统的表达与形态发生有关

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90039-5

Günter Oettling, Heinrich Schmidt, Annamaria Show-Klett, Ulrich Drews

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引用次数: 20

Incorporation of fluorescently labeled actin and tropomyosin into muscle cells 将荧光标记的肌动蛋白和原肌球蛋白掺入肌肉细胞

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90035-8

Jeffrey S. Dome, Balraj Mittal, Mark B. Pochapin , Jean M. Sanger, Joseph W. Sanger

{"title":"Incorporation of fluorescently labeled actin and tropomyosin into muscle cells","authors":"Jeffrey S. Dome, Balraj Mittal, Mark B. Pochapin , Jean M. Sanger, Joseph W. Sanger","doi":"10.1016/0045-6039(88)90035-8","DOIUrl":"10.1016/0045-6039(88)90035-8","url":null,"abstract":"<div><p>The two major proteins in the I-bands of skeletal muscle, actin and tropomyosin, were each labeled with fluorescent dyes and microinjected into cultured cardiac myocytes and skeletal muscle myotubes. Actin was incorporated along the entire length of the I-band in both types of muscle cells. In the myotubes, the incorporation was uniform, whereas in cardiac myocytes twice as much actin was incorporated in the Z-bands as in any other area of the I-band. Labeled tropomyosin that had been prepared from skeletal or smooth muscle was incorporated in a doublet in the I-band with an absence of incorporation in the Z-band. Tropomyosin prepared from brain was incorporated in a similar pattern in the I-bands of cardiac myocytes but was not incorporated in myotubes. These results in living muscle cells contrast with the patterns obtained when labeled actin and tropomyosin are added to isolated myofibrils. Labeled tropomyosins do not bind to any region of the isolated myofibrils, and labeled actin binds to A-bands. Thus, only living skeletal and cardiac muscle cells incorporate exogenous actin and tropomyosin in patterns expected from their known myofibrillar localization. These experiments demonstrate that in contrast to the isolated myofibrils, myofibrils in living cells are dynamic structures that are able to exchange actin and tropomyosin molecules for corresponding labeled molecules. The known overlap of actin filaments in cardiac Z-bands but not in skeletal muscle Z-bands accounts for the different patterns of actin incorporation in these cells. The ability of cardiac myocytes and non-muscle cells but not skeletal myotubes to incorporate brain tropomyosin may reflect differences in the relative actin-binding affinities of non-muscle tropomyosin and the respective native tropomyosins. The implications of these results for myofibrillogenesis are presented.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 1","pages":"Pages 37-52"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90035-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13600929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36

Induction of the heat-shock response by carbon dioxide in Chironomus thummi 二氧化碳对土爪田鼠热休克反应的诱导

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90034-6

Domingo Barettino , Gloria Morcillo , José-Luis Díez

引用次数: 22

Regulation of gene expression in prediapausing embryos of the silkworm, Bombyx mori: pattern of protein synthesis 家蚕预滞育胚胎的基因表达调控:蛋白质合成模式

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90040-1

Corinne Dorel, Madeleine Coulon

{"title":"Regulation of gene expression in prediapausing embryos of the silkworm, Bombyx mori: pattern of protein synthesis","authors":"Corinne Dorel, Madeleine Coulon","doi":"10.1016/0045-6039(88)90040-1","DOIUrl":"10.1016/0045-6039(88)90040-1","url":null,"abstract":"<div><p>Specific qualitative and quantitative changes in protein synthesis occur from the fertilization to the onset of diapause in the silkworm. We have used two-dimensional gel electrophoresis to analyse the patterns of proteins synthesized in prediapausing eggs of <em>Bombyx</em>. This analysis has been carried out with in vivo labelled polypeptides and with proteins synthesized in vitro by RNA isolated at different stages. The oocyte contains an abundant supply of diverse mRNA which are translatable in vitro. A set of proteins with molecular weight range of 68000 to 74000 and isoelectric points of 5.85–5.95 (hereafter referred to as No. 30) is specific of the germ-anlage stage. Transcripts encoding the No. 30 proteins are not detectable in oocytes, and inhibition of transcription by actinomycin D indicates that No. 30 mRNA are synthesized de novo. Treating eggs at the germ-anlage stage with 4 N HCl at 46°C prevents diapause and is accompanied by overproduction of No. 30 protein. The induction of No. 30 synthesis is also the main event of the heat shock response. The implications of these findings in relation to early embryonic development and prevention of diapause are discussed.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 1","pages":"Pages 87-92"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90040-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14496309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Synthesis of specific proteins for sexual development in Dictyostelium discoideum 盘状盘齿龙性发育特异性蛋白的合成

Cell differentiation Pub Date : 1988-03-01 DOI: 10.1016/0045-6039(88)90046-2

Ryoichi Moriyama, Kaichiro Yanagisawa

引用次数: 5