Bruno Péault, Monique Coltey, Nicole M. Le Douarin
{"title":"Ontogenic emergence of a quail leukocyte/endothelium cell surface antigen","authors":"Bruno Péault, Monique Coltey, Nicole M. Le Douarin","doi":"10.1016/0045-6039(88)90069-3","DOIUrl":"10.1016/0045-6039(88)90069-3","url":null,"abstract":"<div><p>The ontogenic emergence of MB1, a quail cell surface antigen expressed by endothelial and hemopoietic cells but not erythrocytes, was followed by direct immunofluorescent staining of transverse sections of the developing blastodisc, from the stage of the cephalic fold until 22 pairs of somites. Along the developmental sequence that leads from hemangioblasts, the mesodermal precursors of both endothelium and hemopoietic cells, to vessels containing blood cells, MB1 is first expressed by arising endothelial cells. These first emerge as flattened cells at the periphery of hemangioblastic clusters in the area opaca from the stage of one pair of somites and slightly later as unicellular angioblasts in the area pellucida and in the embryo. MB1 expression is then maintained on endothelium as vessels develop, in contrast with extraembryonic blood islands in which primitive erythroblasts remain MB1-negative. A small proportion of blood island cells and budding of endothelium contribute a population of MB1-positive hemopoietic cells appearing soon after the onset of angiogenesis.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 3","pages":"Pages 165-174"},"PeriodicalIF":0.0,"publicationDate":"1988-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90069-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14503960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The histones of the sperm of Spisula solidissima include a novel, cysteine-containing H-1 histone","authors":"J. Ausio, K.E. Van Holde","doi":"10.1016/0045-6039(88)90070-X","DOIUrl":"10.1016/0045-6039(88)90070-X","url":null,"abstract":"<div><p>The histones remaining at the end of the spermiogenic differentiation, which are found associated with a highly basic protamine-like component [Ausio, J. and K.E. Van Holde (1987) Eur. J. Biochem. 165, 363–371] in the mature sperm of <em>Spisula solidissima</em>, have been isolated and characterized for the first time. All four core histones H2A, H2B, H3, H4, and the lysine-rich histone H1 are present. The core histones are found in equal stoichiometric amounts. As has been observed in other bivalve molluscs, the amino acid compositions of the core histones of <em>S. solidissima</em> sperm are very close to those of their counterparts in the calf thymus somatic histones. The spermatic histone H1 exhibits an amino acid composition and structural features similar to other histones of the histone H1 family. Yet this latter histone seems to be sperm-specific, and it contains at least two cysteine residues per molecule, which makes it unique in its class.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 3","pages":"Pages 175-189"},"PeriodicalIF":0.0,"publicationDate":"1988-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90070-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14503961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Orlando , R. Geremia , C. Frusciante , B. Tedeschi , P. Grippo
{"title":"DNA repair synthesis in mouse spermatogenesis involves DNA polymerase β activity","authors":"P. Orlando , R. Geremia , C. Frusciante , B. Tedeschi , P. Grippo","doi":"10.1016/0045-6039(88)90075-9","DOIUrl":"10.1016/0045-6039(88)90075-9","url":null,"abstract":"<div><p>The role of DNA polymerase α-DNA primase complex and DNA polymerase β in DNA replication and ultraviolet-induced DNA repair synthesis has been analyzed in mouse spermatogenesis. Autoradiographic experiments with germ cells in culture, indicating an involvement of DNA polymerase α and / or δ in DNA replication, and of DNA polymerase β in DNA repair synthesis, have been confirmed by studying partially purified enzymes. These findings support the idea that, different from other biological systems, in meiotic and post meiotic male mouse germ cells DNA polymerase β is the main DNA polymerase form needed for DNA repair.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 3","pages":"Pages 221-230"},"PeriodicalIF":0.0,"publicationDate":"1988-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90075-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14262311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Degradation of yolk proteins in sea urchin eggs and embryos","authors":"Yukio Yokota , Koichi H. Kato","doi":"10.1016/0045-6039(88)90071-1","DOIUrl":"10.1016/0045-6039(88)90071-1","url":null,"abstract":"<div><p>Yolk granules isolated from unfertilized and fertilized eggs of the sea urchins, <em>Hemicentrotus pulcherrimus</em> and <em>Anthocidaris crassispina</em>, were incubated in acidic media, and the protein components were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. By the incubation, a protein (molecular weight 180 000 in <em>H. pulcherrimus</em> and 178 000 in <em>A. crassispina</em>) most abundant in unfertilized eggs decreased, while proteins (molecular weight 61 000, 72 000, 94 000, 114 000 in <em>H. pulcherrimus</em> and 56 000, 70 000, 92 000, 112 000 in <em>A. crassispina</em>) dominant in developed embryos increased. Neither alkaline nor neutral condition resulted in such changes in the electrophoretic patterns of proteins as observed in acidic media. Experiments with various inhibitors of proteases suggested that thiol protease(s), such as cathepsin B, may be the most important enzyme(s) in the degradation of yolk proteins in embryogenesis of the sea urchin.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 3","pages":"Pages 191-199"},"PeriodicalIF":0.0,"publicationDate":"1988-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90071-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14503962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ATP-γ-S (adenosine 5′-O-(3-thiotriphosphate)) microinjection increases progesterone-stimulated histone kinase activity in Xenopus oocytes","authors":"J.C. Labbé , A. Picard , M. Dorée","doi":"10.1016/0045-6039(88)90072-3","DOIUrl":"10.1016/0045-6039(88)90072-3","url":null,"abstract":"<div><p>Progesterone stimulates the activity of a potent Ca<sup>2+</sup>- and cyclic nucleotide-independent histone kinase in <em>Xenopus</em> oocytes. Microinjection of ATP-γ-S during the course of meiotic maturation or after the oocytes had arrested at second meiotic metaphase resulted in a further increase of this histone kinase activity in progesterone-stimulated oocytes. ATP-γ-S microinjection also increased and stabilized the activity of the maturation-promoting factor. The possible mechanism of ATP-γ-S action is discussed.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 3","pages":"Pages 201-206"},"PeriodicalIF":0.0,"publicationDate":"1988-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90072-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13974504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The expression of glycoproteins in the extracellular matrix of the cellular slime mold Dictyostelium discoideum","authors":"Christopher M. West , Gregory W. Erdos","doi":"10.1016/0045-6039(88)90032-2","DOIUrl":"10.1016/0045-6039(88)90032-2","url":null,"abstract":"<div><p>In this report we examine the accumulation of glycoconjugates in the extracellular medium and insoluble matrices surrounding developing cells of the cellular slime mold <em>Dictyostelium discoideum</em>. Conditions were employed which permitted advanced development (slug stage and beyond) in suspension culture. Under these conditions, up to one-third of the total culture protein appeared as non-sedimentable, extracellular material over the course of 48 h of incubation. Most of the secreted molecules expressed carbohydrate antigens (glycoantigens) as detected by Western blotting, using a panel of six monoclonal antibodies. Since the glycoantigens are secreted, immunoelectron microscopy was used to localize the glycoantigens in the extracellular matrices surrounding normally developing cells, including the slime sheath, stalk tube, inner spore coat, outer spore coat, and intercellular fluid between spores. Each glycoantigen had a characteristic distribution, and each extracellular matrix space contained a unique combination of glycoantigens. Thus, although each of these matrices (except inter-spore fluid) contains cellulose as a primary component, they could be distinguished on the basis of their glycoantigen and, by inference, glycoprotein compositions. Furthermore, there were differences between anterior and posterior regions of both slime sheats and stalk tubes. These observations show that secretion as detected in suspension culture occurs under normal conditions as a part of the process of depositing extracellular matrices around the cells. The distributions show that the cell aggregate positionally regulates the expression and deposition of secretory glycoproteins; the resultant patterns of expression of unique protein-linked carbohydrate structures imply a functional role in matrix organization and possibly cell activity which can now be explored.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 1","pages":"Pages 1-16"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90032-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14497188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Differentiation of cartilage cells studied by quantitative [3H]thymidine autoradiography in vitro","authors":"Erella Livne, Anna Weiss, Michael Silbermann","doi":"10.1016/0045-6039(88)90037-1","DOIUrl":"10.1016/0045-6039(88)90037-1","url":null,"abstract":"<div><p>The apical segments of the mandibular condylar cartilage of newborn ICR mice, containing the intact zones of progenitor cells along with a few rows of chondroblasts were initially prelabelled in vitro with [<sup>3</sup>H]thymidine and were subsequently chased and cultured for as long as eight days. Such explants underwent a process of tissue regeneration, as after three days in culture they reconstituted the original structure of the organ, thus resembling the in vivo appearance of neonatal mandibular condylar cartilage. Cellular proliferation with subsequent differentiation in the regenerating tissue was followed by means of quantitative autoradiography. Immediately after labelling, the autoradiography-positive grains were confined exclusively to progenitor cells. The latter revealed a substantial ability to proliferate in vitro, a fact that was manifested by a progressive increase in the labelling index along the course of the culture. The latter process was followed by cellular differentiation thereby obtaining hypertrophic chondrocytes. The increase in the rate of labelling index and in the total number of [<sup>3</sup>H]thymidine-labelled cells was significantly correlated with the overall growth of the regenerating explants.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"23 1","pages":"Pages 61-67"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90037-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14497193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}