S-Adenosylmethionine, S-adenosylhomocysteine and S-adenosylhomocysteine hydrolase variations during differentiation of Dictyostelium discoideum

Cell differentiation Pub Date : 1988-02-01 DOI:10.1016/0045-6039(88)90012-7

M.C. Guitton , B.T. Keller , D. Part , J. De Gunzburg , R.T. Borchardt , M. Véron

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引用次数: 7

Abstract

We have analyzed the level of substrate (AdoMet) and products (AdoHcy) of transmethylations throughout the developmental cycle of the primitive eukaryote Dictyostelium discoideum. The ratio AdoMet/AdoHcy varied dramatically during differentiation. The intracellular level of AdoHcy decreased sharply after the beginning of starvation reaching a value of 18% of that in vegative cells within 4 h. In contrast, there was a two-fold transient increase in AdoMet at the time of aggregation. However, these changes were not related to changes in AdoHcy hydrolase since constant levels of both the protein and the activity were found until 16 h of differentiation. In particular, there was no indication of an in vivo inactivation of the enzyme by cAMP at the time of aggregation. These results are discussed with respect to the previously postulated role of AdoHcy hydrolase in the regulation of the AdoMet/AdoHcy ratio in eukaryotic cells.

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s -腺苷蛋氨酸、s -腺苷同型半胱氨酸和s -腺苷同型半胱氨酸水解酶在盘状盘基钢的分化过程中的变化

我们分析了原始真核生物盘状盘基钢的整个发育周期中转甲基化的底物(AdoMet)和产物(adhcy)的水平。AdoMet/ adhcy比值在分化过程中变化显著。饥饿开始后，细胞内AdoMet水平急剧下降，在4小时内达到阴性细胞的18%。相反，AdoMet在聚集时短暂增加了两倍。然而，这些变化与adhcy水解酶的变化无关，因为直到分化16小时，蛋白质和活性都保持不变。特别是，在聚合时，没有迹象表明cAMP在体内使酶失活。这些结果讨论了adhcy水解酶在真核细胞AdoMet/ adhcy比例调节中的先前假设的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cell differentiation

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