Cell differentiation最新文献_第7页

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90019-X

引用次数: 0

Morphogenesis of mouse mammary epithelial cells growing within collagen gels: ultrastructural and immunocytochemical characterization 在胶原凝胶中生长的小鼠乳腺上皮细胞的形态发生:超微结构和免疫细胞化学表征

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90011-5

Susan Hamamoto, Walter Imagawa, Jason Yang, Satyabrata Nandi

引用次数: 41

A cell surface phosphoprotein of 48 kDa specific for myoblast fusion 一种48 kDa的细胞表面磷蛋白，用于成肌细胞融合

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90016-4

J.L. Lognonne, J.P. Wahrmann

引用次数: 16

Induction of mesodermal tissues by acidic and basic heparin binding growth factors 酸性和碱性肝素结合生长因子对中胚层组织的诱导作用

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90010-3

Horst Grunz , Wallace L. McKeehan , Walter Knöchel , Jochen Born , Heinz Tiedemann , Hildegard Tiedemann

{"title":"Induction of mesodermal tissues by acidic and basic heparin binding growth factors","authors":"Horst Grunz , Wallace L. McKeehan , Walter Knöchel , Jochen Born , Heinz Tiedemann , Hildegard Tiedemann","doi":"10.1016/0045-6039(88)90010-3","DOIUrl":"10.1016/0045-6039(88)90010-3","url":null,"abstract":"<div><p>The inducing activity of two heparin binding growth factors HBGF-1 (prostate epithelial cell growth factor; acidic p<em>I</em>) and HBGF-2 (fibroblast growth factor; basic p<em>I</em>) from bovine brain has been tested on totipotent ectoderm from early amphibian (<em>Xenopus laevis, Ambystoma mexicanum</em>) embryos. Both factors induced, at high concentrations, mostly compact spheres surrounded by a non-epidermal epithelium. When the concentration or time of incubation was reduced, large muscle inductions frequently organized as somites were formed besides endothelial vesicles, mesenchyme and smaller areas of intestine-like epithelium. Further reduction of the concentrations or the time of incubation led to an increase in size and number of endothelium-lined vesicles and of mesenchyme, whereas the induction of muscle decreased. At still lower concentrations the overall rate of inductions decreased. The relationship of the growth factors to the vegetalizing factor from chicken embryos, dilution of which shows a similar shift in induced organs, is discussed. The present and previous experiments suggest that different mesodermal and endodermal tissues are induced by secondary interactions in which additional factors are involved. The induced organs derive from dorsal as well as from ventral mesoderm.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 3","pages":"Pages 183-189"},"PeriodicalIF":0.0,"publicationDate":"1988-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90010-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13598408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 67

The differentiation behaviour of MDCK cells grown on matrix components and in collagen gels MDCK细胞在基质成分和胶原凝胶中的分化行为

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90013-9

W. Karst, H.-J. Merker

{"title":"The differentiation behaviour of MDCK cells grown on matrix components and in collagen gels","authors":"W. Karst, H.-J. Merker","doi":"10.1016/0045-6039(88)90013-9","DOIUrl":"10.1016/0045-6039(88)90013-9","url":null,"abstract":"<div><p>MDCK cells are grown on various substrates (Thermanox pure, extracellular matrix (ECM), dried or wet collagen type I or type III), on floating collagen and enclosed in collagen gels, and their differentiation behaviour is investigated electron microscopically. The cells grown on ECM or dried collagen (type I and type III) do not show any changes as compared with the controls (Thermanox). Differentiation processes can only be observed when the cells are grown on wet collagen (type I and type III), especially on floating collagen and enclosed in collagen gels. These differentiation processes comprise changes in the cell shape, an increase in the number of microvilli, an increase in the length of the lateral contact zone with the formation of gap junctions and desmosomes, and an increase in the number and size of the cell organelles. A basement membrane only develops in the form of short segments. Moreover, on floating collagen and in collagen gels three-dimensional, organoid structures develop: cell aggregates with central lumina and tubuli. They are formed by cuboid cells that also exhibit indications of differentiation. Basement membrane fragments occur more often and are longer. It can be concluded from these findings that the chemical structure of the substrate does not play the primary role in the described processes. It is rather the physical properties, probably the plasticity, that are of significance. Due to this property the cells change their shape and the contact areas increase in size. The establishment of contacts might be the triggering factor for differentiation. Organoid structures with lumina develop when the apical surface comes into contact with other cells or collagen gels. The pronounced tendency towards polarization necessitates a re-arrangement of three-dimensionally growing cells to structures with lumina. The formation of the basement membrane is the result and not the cause of differentiation.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 3","pages":"Pages 211-224"},"PeriodicalIF":0.0,"publicationDate":"1988-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90013-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14482875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Subject index volume 22 主题索引第22卷

Cell differentiation Pub Date : 1988-02-01 DOI: 10.1016/0045-6039(88)90020-6

引用次数: 0

Distribution of R-cognin and choline acetyltransferase in the ganglion cell layer of developing chick neural retina r -认知素和胆碱乙酰转移酶在发育中的鸡神经视网膜神经节细胞层的分布

Cell differentiation Pub Date : 1988-01-01 DOI: 10.1016/0045-6039(88)90023-1

E.T. Dobi, F.J. Naya, R.E. Hausman

引用次数: 12

Electrophoretic pattern of NADPH-dependent oxidoreductive activities in K 562 and HL 60 leukemic cell lines k562和hl60白血病细胞系nadph依赖性氧化还原活性的电泳图谱

Cell differentiation Pub Date : 1988-01-01 DOI: 10.1016/0045-6039(88)90027-9

Roberto Ravazzolo, Renata Barresi, Cecilia Garré, Giovanna Bianchi-Scarrá, Franco Ajmar

引用次数: 1

Acknowledgement 确认

Cell differentiation Pub Date : 1988-01-01 DOI: 10.1016/0045-6039(88)90030-9

Lauri Saxén (Editor-in-Chief)

引用次数: 0

Developmental control of the expression of the dihydroorotate dehydrogenase and UMP synthase genes in Dictyostelium discoideum 二氢乳酸菌脱氢酶和UMP合成酶基因表达的发育调控

Cell differentiation Pub Date : 1988-01-01 DOI: 10.1016/0045-6039(88)90028-0

Michel Faure, Monique Kalekine, Emmanuelle Boy-Marcotte, Michel Jacquet

{"title":"Developmental control of the expression of the dihydroorotate dehydrogenase and UMP synthase genes in Dictyostelium discoideum","authors":"Michel Faure, Monique Kalekine, Emmanuelle Boy-Marcotte, Michel Jacquet","doi":"10.1016/0045-6039(88)90028-0","DOIUrl":"10.1016/0045-6039(88)90028-0","url":null,"abstract":"<div><p>Developmental variations in the expression of two genes of the de novo pyrimidine biosynthetic pathway have been examined in <em>Dictyostelium discoideum</em>. One gene, DdPYR4, encodes the dihydroorotate dehydrogenase (EC 1.3.3.1); the other, DdPYR5-6, encodes the UMP synthase which in <em>D. discoideum</em> is a bifunctional enzyme harboring both the orotate phosphoribosyl transferase activity (EC 2.4.2.10) and the OMP decarboxylase activity (EC 4.1.1.23). The relative amount of mRNA for both genes has been estimated by hybridization with the previously cloned DNAs and compared with the amount of actin mRNA. The level of both mRNAs is dramatically reduced after 4 h of development and remains at a low level later in development. In contrast to these variations, the specific activity of the enzymes encoded by these genes during development is similar to that measured during exponential growth. These results lead us to propose that DdPYR4 and DdPYR5-6 genes encode for relatively stable proteins and that their synthesis is reduced to maintain a constant level of enzymes in non-growing cells. This mode of regulation could apply to a large number of housekeeping genes.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 2","pages":"Pages 159-164"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90028-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13968093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7