Cell differentiation最新文献

{"title":"Developmental modifications of sheep kidney antigens","authors":"J.J. Candelier ,&nbsp;P. Gane ,&nbsp;P. Couillin ,&nbsp;J. Martal ,&nbsp;R. Oriol","doi":"10.1016/0045-6039(88)90024-3","DOIUrl":"10.1016/0045-6039(88)90024-3","url":null,"abstract":"<div><p>We have used monoclonal antibodies to study the changes in the expression of four kidney antigens during organogenesis in the sheep. Two of these antibodies, EE24.6 and EJ30.1, label intensely only the adult kidney, whereas the other two, EK17.1 and EJ15.1, bind to the extracellular matrix of the embryonic kidney. For EJ15.1, the staining of the extracellular matrix decreases temporarily during the second half of intrauterine life, a period during which a light staining appears in the mesangium. For the other, EK17.1, the extracellular matrix staining in the stroma gradually decreases as the embryo grows, while staining of the mesangium and the arterial intima becomes evident. With EK17.1, fibronectin is identified in the extracellular matrix of the embryonic kidney and intracellularly in the mesangial cells after these cells have colonized the glomerulus. The mesonephros staining seems to be the same as that of the metanephros.</p><p>In the adult, extraglomerular vascular endothelial cells bind EK17.1, whereas intraglomerular endothelial cells do not express fibronectin, which suggests a functional difference between endothelial cells in these two localizations.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 2","pages":"Pages 125-134"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90024-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14468707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chicken blastoderm: current views on cell biological events guiding intercellular communication","authors":"Fernand Harrisson ,&nbsp;Luc Andries ,&nbsp;Lucien Vakaet","doi":"10.1016/0045-6039(88)90021-8","DOIUrl":"10.1016/0045-6039(88)90021-8","url":null,"abstract":"","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 2","pages":"Pages 83-105"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90021-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14405758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concanavalin A induces neural tissue and cartilage in amphibian early gastrula ectoderm","authors":"Alexander T. Mikhailov,&nbsp;Nikolai A. Gorgolyuk","doi":"10.1016/0045-6039(88)90026-7","DOIUrl":"10.1016/0045-6039(88)90026-7","url":null,"abstract":"<div><p>We have studied in vitro differentiation of explants of the amphibian (<em>Rana temporaria</em>) early gastrula ectoderm after treatment with various concentrations (50–300 μg/ml) of ‘free’ and Sepharose-bound concanavalin A (Con A). The explants were incubated with Con A for 3 h at 20°C; the rolling up of the explants was prevented by using special weights. We have demonstrated that: (1) free Con A has an inducing action on the explants in the concentration range 100–300 μg/ml medium; (2) when treated with Con A the explants produce neural tissue (50–70%), cartilage (20–40%) and, rarely, lentoids (5–10%); (3) the frequency of neural and cartilage inductions was similar at various Con A concentrations; (4) α-methyl-<span>d</span>-mannoside pyranoside inhibited the Con A effects; (5) Sepharose-bound Con A had no effect on the explants, although it was bound to the cell surface of the ectoderm inner layer. Possible mechanisms of the neuralizing and chondrogenic effects of Con A on ectodermal explants are discussed.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 2","pages":"Pages 145-153"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90026-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14386370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of saccharides and sodium chloride on growth and differentiation of Trypanosoma cruzi","authors":"F.J. Adroher ,&nbsp;J.A. Lupiáñez ,&nbsp;A. Osuna","doi":"10.1016/0045-6039(88)90029-2","DOIUrl":"10.1016/0045-6039(88)90029-2","url":null,"abstract":"<div><p>The influence of saccharides, especially glucose and fructose, on the metacyclogenesis and growth of <em>Trypanosoma cruzi</em> has been investigated. In the absence of glucose and fructose in the media, both the percentage of metacyclic forms and the growth increased significantly. Furthermore, the addition of NaCl to the medium without monosaccharides strongly increased the formation of metacyclic forms. Presence of NaCl and absence of monosaccharides showed a synergic effect on differentiation of <em>T. cruzi</em>.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 2","pages":"Pages 165-170"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90029-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14405757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulation of collagen production in vitro by ascorbic acid released from explants of migrating avian neural crest","authors":"Chaya Kalcheim ,&nbsp;Vincent Leviel","doi":"10.1016/0045-6039(88)90022-X","DOIUrl":"10.1016/0045-6039(88)90022-X","url":null,"abstract":"<div><p>Embryonic neuronal tissues contain a collagen-stimulating factor, shown to enhance the hydroxylation and secretion of proline-containing macromolecules by cultured muscle cells. Here we report on a similar activity found during avian embryonic development in explants of migrating mesencephalic neural crest. The degree of proline hydroxylation of proteins secreted into the medium was stimulated 2.5–6-fold in neural crest-muscle and neural crest-somite cocultures, as compared with control cultures devoid of crest explants. No such stimulation occurred when cocultures were treated with the enzyme ascorbate oxidase (EC 1.10.3.3), suggesting that the active factor in neural crest explants was ascorbic acid or an ascorbate-like molecule. Further characterization of this molecule was performed in crest explants and other embryonic tissues by using HPLC with amperometric detection: this study revealed that migrating cephalic neural crest contains 1.5 μg ascorbic acid per mg protein. Our results suggest that ascorbic acid and/or related molecule(s) could act during development of the nervous system as a trigger for collagen production and subsequent assembly of an extracellular matrix.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 2","pages":"Pages 107-114"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90022-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13968092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differentiation of intestinal and ectopic endocrine cells from avian gastric and pancreatic endoderm","authors":"Ann Andrew ,&nbsp;B.B. Rawdon ,&nbsp;Beverley Kramer","doi":"10.1016/0045-6039(88)90025-5","DOIUrl":"10.1016/0045-6039(88)90025-5","url":null,"abstract":"<div><p>The chorio-allantoic grafts analysed were prepared from avian proventricular endoderm combined with its own or pancreatic mesenchyme and from re-associated pancreatic layers. Intestine developed ectopically in some grafts: in these, endocrine cells typical of intestine differentiated irrespective of the source of the endoderm or mesenchyme. In addition, endocrine cells inappropriate for the surrounding histology were detected in small numbers in grafts of all categories. Clearly it is not the mesenchyme that is responsible but perhaps some aspect of the procedure, which may relate to stressful stimuli thought to provoke intestinal metaplasia. The differentiation of inappropriate cells aids in understanding the occurrence of ectopic endocrine tumours.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 2","pages":"Pages 135-144"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90025-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14468708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The developmental fate of dictyostelium discoideum cells depends greatly on the cell-cycle position at the onset of starvation","authors":"T. Ohmori,&nbsp;Y. Maeda","doi":"10.1016/0045-6039(87)90409-X","DOIUrl":"10.1016/0045-6039(87)90409-X","url":null,"abstract":"<div><p>The relationship between the development of <em>Dictyostelium discoideum</em> Ax-2 and the cell cycle at the onset of starvation was analysed with special reference to sorting behaviors during the formation of polarized cell masses (slugs), using a method for inducing good synchrony. Cells starved at different cell-cycle positions showed different developmental features during further culture. For example, cells just before mitosis and dividing cells were sorted out into the anterior prestalk zone of migrating slugs, while cells starved during most of the G₂-phase, into the posterior prespore zone. Time courses of cell aggregation and tip formation were also found to vary greatly in a cell-cycle-related manner, and cells starved during the late G₂-phase showed the most rapid development. Differential chemotaxis and cohesiveness are generally considered to be important for cell sorting in <em>Dictyostelium</em> development. In fact, remarkable differences in the chemotactic ability to a chemoattractant, cAMP, were detected among cells starved at any particular phase of the cell cycle. EDTA-resistant cohesiveness was also acquired differently depending on the cell cycle, and it was stronger in the cells showing more rapid aggregation. These findings indicate a close relation of the cell cycle to the cell sorting and pattern formation. The possible significance of the cell-cycle-related events presented here is discussed, with special emphasis on the process of cell aggregation.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 1","pages":"Pages 11-18"},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(87)90409-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14810872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prealbumin gene expression during mouse development studied by in situ hybridization","authors":"Tatsufumi Murakami ,&nbsp;Yoshiaki Yasuda ,&nbsp;Shuji Mita ,&nbsp;Shuichiro Maeda ,&nbsp;Kazunori Shimada ,&nbsp;Toyoaki Fujimoto ,&nbsp;Shukuro Araki","doi":"10.1016/0045-6039(87)90408-8","DOIUrl":"10.1016/0045-6039(87)90408-8","url":null,"abstract":"<div><p>Localization of prealbumin mRNA in tissues from mice at various stages of gestation was investigated using in situ hybridization procedures. Prealbumin mRNA was detected as early as the 10th day of gestation. It was specifically localized in endodermal cells of the visceral yolk sac, tela choroidea, and hepatocytes. In the adult mice, prealbumin mRNA was localized in the hepatocytes and choroid plexus epithelial cells. These observations indicate that synthesis of prealbumin mRNA is initiated in several different types of cells at early stages of fetal development.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 1","pages":"Pages 1-9"},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(87)90408-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14810871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence for a cyclic renewal of lymphocyte precursor cells in the embryonic chick thymus","authors":"Monique Coltey ,&nbsp;Francine V. Jotereau ,&nbsp;Nicole M. Le Douarin","doi":"10.1016/0045-6039(87)90414-3","DOIUrl":"10.1016/0045-6039(87)90414-3","url":null,"abstract":"<div><p>Experiments involving sequential transplantations of the chick embryonic thymus at E9 to E12 into a first 3-day host quail embryo and then into a second chick host allowed demonstration of the cyclic periodicity of hemopoietic cell seeding of the embryonic thymus. After a first wave of colonization occurring between E6.5 and E8, the thymus becomes refractory to hemopoietic cell entry for about 4 days. It resumes its capacity to be seeded by a second wave of blood-borne stem cells at E12. After a second period of non receptivity starting at E14, a third wave of incoming cells reaches the thymus around E18. Therefore, with a slightly different periodicity, the same cyclic mechanism regulates the renewal of lymphocytes in chick and quail embryos. Quail hemopoietic cells were immunostained in the chimeric thymuses, with a species specific monoclonal antibody (anti-MB1) which recognizes a common surface antigenic determinant on all endothelial and blood cells of the quail (except erythrocytes). Two steps could thus be distinguished in the seeding process. When the thymus becomes receptive for hemopoietic cells, the latter first accumulate in the intrathymic blood vessels before penetrating massively in the thymic parenchyma. The quail chick-chimera system combined with the use of a species- and cell-type-specific antibody provides a unique tool for studying thymic colonization by lymphocyte precursors.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 1","pages":"Pages 71-82"},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(87)90414-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14810006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucose metabolism in transdifferentiating and glucose-blocked cultures of chick embryo neuroretinal cells: an inverse relationship between glycogen and δ-crystallin accumulation","authors":"Saleh A.M. Karim,&nbsp;Michel Flor-Henry,&nbsp;David I. de Pomerai","doi":"10.1016/0045-6039(87)90411-8","DOIUrl":"https://doi.org/10.1016/0045-6039(87)90411-8","url":null,"abstract":"<div><p>Chick embryo neuroretinal (NR) cells transdifferentiate extensively into lens when cultured for several weeks in low-glucose (FH) medium, but fail to do so when high levels of supplementary glucose (FHG) are present. We show here that most aspects of glucose metabolism are promoted in high-glucose cultures, including lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) activities, 2-deoxyglucose uptake, pentose shunt activity and lactate production. Continuous supplementation of high-glucose cultures with low levels of ouabain (FHGO) significantly lowers 2-deoxyglucose uptake, from FHG levels down towards FH levels, especially during the early stages of NR culture. Much later, extensive transdifferentiation into lentoids (with concomitant δ-crystallin accumulation) occurs in these FHGO cultures, which thus resemble FH rather than FHG controls. Another parameter strongly affected by ambient glucose levels is the accumulation of glycogen. Both glycogen itself and glycogen synthetase activity increase steadily in FHG cultures, but decrease slightly under FH conditions. Glycogen accumulation in FHG cultures is largely confined to glial-like cells, particularly those underlying clusters of neurones. Other studies have shown that glial differentiation in vitro is promoted by histotypic interactions with retinal neurones. Thus high glucose may act in concert with neuronal influences to stimulate or stabilize the normal differentiation of retinal glial cells, whose characteristic features in vivo include glycogen synthesis and storage. Furthermore, we show that supplementation of high-glucose cultures with forskolin or dibutyryl cyclic AMP (both of which promote glycogenolysis) results in a slower rate of glycogen accumulation and in enhanced transdifferentiation into lens. In both respects, the forskolin- and dibutyryl cAMP-supplemented FHG cultures are intermediate between FH and FHG controls. Thus the enhancement of normal glial differentiation in NR cultures by high glucose may inhibit or preclude subsequent transdifferentiation into lens.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"22 1","pages":"Pages 29-45"},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(87)90411-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91993890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}