Cell and tissue kinetics最新文献_第4页

Cell growth and cell division: dissociated and random initiated? A study performed on embryonal carcinoma cell lines. II. 细胞生长和细胞分裂:游离和随机启动?胚胎癌细胞系的研究。2

Cell and tissue kinetics Pub Date : 1986-01-01

R Sennerstam, J O Strömberg

引用次数: 0

Epidermal tissue homeostasis. II. Cell pool size, cell birth rate and cell loss in toads deprived of the pars distalis of the pituitary gland. 表皮组织稳态。2剥夺垂体远端部蟾蜍的细胞池大小、细胞出生率和细胞损失。

Cell and tissue kinetics Pub Date : 1985-09-01

P E Budtz

引用次数: 0

Epidermal tissue homeostasis. I. Cell pool size, cell birth rate and cell loss by moulting in the intact toad, Bufo bufo. 表皮组织稳态。完整蟾蜍，Bufo Bufo的细胞池大小，细胞出生率和细胞脱落。

Cell and tissue kinetics Pub Date : 1985-09-01

P E Budtz

{"title":"Epidermal tissue homeostasis. I. Cell pool size, cell birth rate and cell loss by moulting in the intact toad, Bufo bufo.","authors":"P E Budtz","doi":"","DOIUrl":"","url":null,"abstract":"Toad epidermis is a suitable model for studies on tissue homeostasis because cell pool size, influx into and efflux from the cell pool can be easily determined. The cell pool size was obtained by cell counting on photomicrographs, the influx (cell birth rate) was assessed by the metaphase-arrest technique, and the efflux (cell loss by moulting) assessed by counting the number of cells in the corneal layer and recording of intermoult periods. The importance of the methods for assessing these parameters per square unit of skin surface is emphasized. These parameters were studied in eight groups of ten adult male toads sacrificed at various hours of the day. There were minor variations in the cell birth rate, fluctuating around a mean of 26 cells/mm2/hr (obtained at the metaphase collection period from 11.00-14.00 hours). By summation of the cell productions during the eight metaphase collection periods of 3 hr, and extrapolation to an intermoult period (time between two moults), a calculated cell production of about 6340 cells/mm2 in 10.3 days was obtained, whereas the cell loss at each moult was only 2370 cells/mm2. Thus the cell production rate exceeds the rate of cell loss through moults by a factor of 2.7 Arguments are presented that the 'surplus' of cells produced cannot be permanently accommodated within the living epidermis. Consequently a cell deletion rate beyond that by moulting of about 4000 cells/mm2 in 10.3 days or 16 cells/mm2/hr can be calculated. These results are discussed in relation to current concepts of tissue homeostatic mechanism(s). The results are consistent with the hypothesis that controlled cell deletion may be a tissue homeostatic mechanism complementary to controlled cell divisions.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"18 5","pages":"521-32"},"PeriodicalIF":0.0,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15043789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell kinetics from double labeling. 双重标记的细胞动力学。

Cell and tissue kinetics Pub Date : 1985-07-01

H Knolle

引用次数: 0

Kinetic analysis of drug-induced G2 block in vitro. 药物诱导G2阻滞体外动力学分析。

Cell and tissue kinetics Pub Date : 1985-01-01

M Kimmel, F Traganos

{"title":"Kinetic analysis of drug-induced G2 block in vitro.","authors":"M Kimmel, F Traganos","doi":"","DOIUrl":"","url":null,"abstract":"The data on cell-cycle effects of two prospective antitumour agents, (+)-1,2,-bis(3,5-dioxopiperazine-1-yl)propane (soluble ICRF; NSC 169780) and 1,4-bis(2'chloroethyl)-1,4-diazabicyclo [2.2.1] heptane diperchlorate (CBH; NSC 57198) were used to determine whether a modified stathmokinetic experiment could predict the effects of continuous, long-term (0-48 hr) drug exposure in an in vitro L1210 murine leukaemia cell system. Generally, continuous drug exposure of exponentially growing cells does not provide sufficient quantitative information concerning cell-cycle-phase-specific mechanisms of drug action. Alternatively, stathmokinetic experiments, which are usually limited to some fraction of one cell doubling time, provide little information about long-term drug effects. By using mathematical models constructed for this purpose, however, stathmokinetic data can predict the overall proportion of cells affected by a drug though failing to discern between various kinds of drug action (e.g. reversible v. irreversible block, blocking v. killing action, etc.), especially when it occurs in G2 phase. In addition, it can be shown that for at least one of the drugs (soluble ICRF) the stathmokinetic experiment fails to predict 'after-effects' of drug treatment which extend into the following cell cycle(s). It also becomes clear that the degradation of exponential growth characteristics of quickly dividing cells during long-term, continuous drug exposure makes prediction of cell-cycle kinetic perturbations uncertain when derived from short-duration stathmokinetic experiments. However, with care, the joint application of 'short term' (e.g. stathmokinesis) and 'long term' (e.g. continuous exposure) techniques allow adequate quantitative insight into drug-perturbed cell-cycle kinetics. The applicability of modelling techniques is discussed: in the present instance it is limited to lower drug concentrations. For higher drug concentrations, effects like increased ploidy, ineffective division, etc., make it impossible in the present study to obtain a clear picture of the kinetics.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"18 1","pages":"91-110"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15035037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abundance of tubulin mRNA on polysomes following deciliation and during the synchronous cell cycle of Tetrahymena. 在四膜虫的同步细胞周期中，多体上的微管蛋白mRNA的丰度。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00612.x

R C Bird, A M Zimmerman

{"title":"Abundance of tubulin mRNA on polysomes following deciliation and during the synchronous cell cycle of Tetrahymena.","authors":"R C Bird, A M Zimmerman","doi":"10.1111/j.1365-2184.1984.tb00612.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1984.tb00612.x","url":null,"abstract":"The protozoan, Tetrahymena pyriformis GL, was used as a model system for studying polysomal mRNA during the cell cycle and during cilia regeneration. Our previous work has shown a substantial induction of tubulin synthesis following deciliation and during G2 of the synchronous cell cycle. In the present study, the abundance of tubulin mRNA on polysomes was examined in order to determine whether more tubulin mRNA was being translated during the periods of peak tubulin synthesis. Polysomes isolated at sequential times following deciliation and during the synchronous cell cycle were translated in a cell-free translation system derived from wheat germ. The abundance of tubulin mRNA on polysomes was inferred from the amount of tubulin translated in vitro. Following deciliation and prior to the peak period of tubulin synthesis, the abundance of tubulin mRNA (at 140 min post-deciliation) increases to 25 times the initial value observed (at 20 min post-deciliation). Since the increase in tubulin mRNA abundance precedes the peak in tubulin synthesis, induction of tubulin synthesis appears to be mRNA-dependent. A similar analysis of tubulin mRNA abundance on polysomes during the synchronous cell cycle revealed a peak of tubulin mRNA prior to each peak of tubulin synthesis. These studies suggest that the periodic fluctuations in the synthesis of tubulin are dependent upon fluctuating levels of tubulin mRNA on polysomes.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"17 5","pages":"535-44"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1984.tb00612.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17490223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cultured human tumour cells may be arrested in all stages of the cycle during stationary phase: demonstration of quiescent cells in G1, S and G2 phase. 培养的人肿瘤细胞可以在固定期的所有周期阶段被捕获:在G1, S和G2期显示静止细胞。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00604.x

B Drewinko, L Y Yang, B Barlogie, J M Trujillo

{"title":"Cultured human tumour cells may be arrested in all stages of the cycle during stationary phase: demonstration of quiescent cells in G1, S and G2 phase.","authors":"B Drewinko, L Y Yang, B Barlogie, J M Trujillo","doi":"10.1111/j.1365-2184.1984.tb00604.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1984.tb00604.x","url":null,"abstract":"Six human colon carcinoma cell lines were induced to enter stationary phase of growth by nutrient deprivation and cell crowding. Growth kinetics parameters (cell number, flow cytometric analysis of DNA distribution, and labelling and mitotic indices) were measured sequentially for all lines during the various stages of in vitro growth. Our results demonstrated that a substantial fraction of cells (9–18%) were located in G2, phase when they changed from an exponential to a stationary mode of growth. Moreover, a large number of cells in stationary phase of growth had an S‐phase DNA content, as determined by flow cytometry, but failed to incorporate radioactive DNA precursors (up to 15‐fold difference). to substantiate these findings. cells in stationary phase of growth were induced to enter exponential growth by re‐seeding in fresh medium at a lower density. Subsequently observed changes in DNA‐compartment distribution, and in labelling and mitotic indices were those expected from cells that had been arrested at different stages of the cycle during their previous stationary phase. Thus, the non‐proliferating quiescent state (Q), traditionally located ‘somewhere’ in G1, phase, appears to be composed also of cells that can be arrested at other stages of the cycle (Qs, and QG). Although the proportion of such cells is rather small, their contribution to the growth kinetics behaviour of human in vivo tumours will become apparent following ‘recruiting’ or ‘synchronizing’ clinical manoeuvres and will prevent the formation of a clear‐cut wave of synchronized cells.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"17 5","pages":"453-63"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1984.tb00604.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17526929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59

Circadian-stage dependence of methotrexate in a keratinized epithelium. An in-vivo study using flow cytometry on the hamster cheek pouch epithelium. 甲氨蝶呤在角质化上皮中的昼夜周期依赖性。流式细胞术在仓鼠颊袋上皮的体内研究。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00607.x

U Møller, J K Larsen, N Keiding, I J Christensen

{"title":"Circadian-stage dependence of methotrexate in a keratinized epithelium. An in-vivo study using flow cytometry on the hamster cheek pouch epithelium.","authors":"U Møller, J K Larsen, N Keiding, I J Christensen","doi":"10.1111/j.1365-2184.1984.tb00607.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1984.tb00607.x","url":null,"abstract":"The partially synchronized cell system of the hamster cheek pouch epithelium shows a characteristic diurnal rhythm of cell proliferation. Bolus injections of methotrexate (Mtx) in both lethal (10 g/m2) and non-lethal (2 g/m2) doses were found to inhibit cell-cycle progression primarily by impairing the G1/S transition. The results were obtained by flow cytometric DNA analysis. The inhibitory effect of Mtx manifested itself as a relative decrease of the S fraction (drug-effector phase), and was found to be dependent both on the dose and on the time of the day it was given. A bolus injection of Mtx was given either at 1200 hr (when a minimal number of cells are in S phase) or at 0200 hr (when a maximum number of cells are in S phase). The greatest cumulative decrease in S fraction was seen when the injection was given at 1200 hr. The time between injection and the effect (seen as a decrease in S fraction) was independent of the time of the Mtx injection, but seemed instead to be related to the natural diurnal period of increasing flux from G1 to S phase (at the onset of the dark period). The main effect (the relative decrease in S fraction) was repeated during the following 24-hr period, pointing to a protracted effect of Mtx on G1 cells. G1 cells affected by the initial high Mtx plasma concentration seem to be responsible for the reduced influx into S phase in both the first and second 24-hr period. In earlier toxicological studies, the survival rate of hamsters was dependent on the time of injection and was highest after injection at 1200 hr. Thus maximum cytokinetic effect on epithelial cells was found at the time of the day when there was a minimum lethal effect on the animal.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"17 5","pages":"483-95"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1984.tb00607.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17270438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effect of methotrexate on cells in a keratinized epithelium with an active thymidine salvage pathway assessed by autoradiography. 甲氨蝶呤对角化上皮细胞的影响，通过放射自显影评估胸苷残留通路。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00608.x

U Møller, N Keiding, J K Larsen

{"title":"Effect of methotrexate on cells in a keratinized epithelium with an active thymidine salvage pathway assessed by autoradiography.","authors":"U Møller, N Keiding, J K Larsen","doi":"10.1111/j.1365-2184.1984.tb00608.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1984.tb00608.x","url":null,"abstract":"In the partially synchronized cell system of the hamster cheek pouch epithelium, the inhibitory effect of a bolus injection of methotrexate (Mtx) (2 g/m2, injected at 1200 hr) was analysed by means of both autoradiography and flow cytometry (FCM) in a 21-hr experiment. For autoradiography [3H]TdR and [3H]UdR were used as tracers for salvage and de novo pathways of thymidylate (TMP) synthesis, respectively. For FCM no tracers were injected. The autoradiographic studies demonstrated an active TdR salvage pathway for DNA synthesis, not affected by the impaired de novo TMP synthesis. The blocked de novo TMP synthesis was partially released 7 hr after Mtx injection, but it had not totally recovered at the end of the experiment. The decrease in the fraction of S-phase cells detected about 10 hr after Mtx injection by autoradiographic labelling with [3H]TdR and by FCM was found to be caused by a decrease in the number of cells entering S phase. However, Mtx did not influence the salvage TMP synthesis rate of cells entering S phase.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"17 5","pages":"497-507"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1984.tb00608.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17270439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Morphometric and kinetic studies on the changes induced in the intestinal mucosa of rats by intraperitoneal administration of quinacrine. 阿奎宁腹腔注射对大鼠肠黏膜变化的形态学及动力学研究。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00603.x

P V Senior, J P Sunter, D R Appleton, A J Watson

{"title":"Morphometric and kinetic studies on the changes induced in the intestinal mucosa of rats by intraperitoneal administration of quinacrine.","authors":"P V Senior, J P Sunter, D R Appleton, A J Watson","doi":"10.1111/j.1365-2184.1984.tb00603.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1984.tb00603.x","url":null,"abstract":"The intraperitoneal administration of large doses of quinacrine in rats results in a state of enteromegaly affecting mainly the distal small bowel, caecum and proximal colon. This enteromegaly is associated with mucosal crypt hyperplasia, and hypertrophy and hyperplasia of the Muscularis propria. In order to investigate the changes in the intestinal mucosal crypts, morphometry and a metaphase-arrest experiment with vincristine were undertaken on a group of rats given 12 mg of quinacrine hydrochloride by intraperitoneal injection daily for 5 days 2 weeks previously, and comparisons drawn with a group of control animals. In the quinacrine-treated animals there was marked enteromegaly affecting the distal small bowel, caecum and proximal colon, and in these segments there was clear crypt hyperplasia. Proximal and distal to the dilated bowel hyperplasia was not seen. No consistent pattern of change in crypt-cell birth rate was evident. The mechanisms by which quinacrine may effect kinetic and morphometric changes in the intestinal crypts are considered.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"17 5","pages":"445-52"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1984.tb00603.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17526928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2