Cell and tissue kinetics最新文献_第3页

Prolongation of cell cycle transit time and the presence of non-cycling cells in human lymphoblastoid cells cultured under adverse conditions. 在不利条件下培养的人淋巴母细胞样细胞中细胞周期传递时间的延长和非循环细胞的存在。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01311.x

C L Villiers, F K Adam, N Miller, D Brown, N C Hughes-Jones

引用次数: 4

Cytotoxic effect and induction of sister chromatid exchange in exponentially growing rat 9L gliosarcoma cells after brief exposure to BrdU. 短暂暴露于BrdU后指数生长大鼠9L胶质瘤细胞的细胞毒作用和诱导姐妹染色单体交换。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01317.x

R X Zhang, T Nagashima, T Hoshino

{"title":"Cytotoxic effect and induction of sister chromatid exchange in exponentially growing rat 9L gliosarcoma cells after brief exposure to BrdU.","authors":"R X Zhang, T Nagashima, T Hoshino","doi":"10.1111/j.1365-2184.1987.tb01317.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1987.tb01317.x","url":null,"abstract":"The magnitude of DNA modulation in rat 9L gliosarcoma cells after a brief exposure to bromodeoxyuridine (BrdU) was studied by assaying colony-forming efficiency (CFE) and the number of sister chromatid exchanges (SCEs) per metaphase. The CFE assay showed that a 1-hr exposure to BrdU, at concentrations ranging from 10 to 1000 microM, produced a maximum cell kill of 5%. After a 2-hr exposure to 20 microM BrdU, the surviving fraction was 0.99, and even at a BrdU concentration of 1000 microM, 77% of the 9L cells survived. Compared with control cultures, the relative number of SCEs per metaphase in treated cultures was increased after a 1-hr exposure to BrdU at concentrations of 100 microM or more and after a 2-hr exposure to concentrations of 20 microM or more; no increase was observed in cells treated for 30 min with BrdU at concentrations up to 1000 microM. When the treated cells were allowed to grow in BrdU-free growth medium, the number of SCEs per metaphase returned to the control level within 24 hr, even after exposure to BrdU at concentrations as high as 1000 microM. These results demonstrate that exposure to BrdU at concentrations of up to 1000 microM for 30 min, 100 microM for 1 hr, and 20 microM for 2 hr causes little modulation of DNA.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"20 3","pages":"357-62"},"PeriodicalIF":0.0,"publicationDate":"1987-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1987.tb01317.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14809723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Sensitivity to X-irradiation in relation to cell size of CHO cells synchronized in early G1. G1早期同步的CHO细胞对x照射的敏感性与细胞大小的关系。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01318.x

R A Tobey, H A Crissman, M E Wilder, F Traganos, Z Darzynkiewicz

引用次数: 2

Inhibition of hepatocyte proliferation in vivo by a glycopeptide from rat serum. 大鼠血清糖肽对肝细胞增殖的抑制作用。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01315.x

C Nadal

{"title":"Inhibition of hepatocyte proliferation in vivo by a glycopeptide from rat serum.","authors":"C Nadal","doi":"10.1111/j.1365-2184.1987.tb01315.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1987.tb01315.x","url":null,"abstract":"The activity of a glycopeptide prepared from rat serum by treatment with trypsin and ultrafiltration was investigated in several in vivo proliferation systems. In baby rat hepatocytes synchronized by a subcutaneous injection of casein solution it caused a G1-S block, stopping cells at the end of the G1 phase and sending them back to the G0 phase. The glycopeptide also caused a G1-S block in young adult rats during the first semi-synchronized wave of proliferation that followed partial hepatectomy. Inhibition of hepatocyte proliferation by the glycopeptide was suppressed by blood proteins from normal rats but not from acute phase rats. Alpha 1-acid glycoprotein, an acute phase protein, increased this inhibition and reversed the antagonistic effect of normal blood proteins. In normal baby rats a G1-S block of non-synchronously proliferating hepatocytes was produced in two situations in which the antagonistic effect of normal blood proteins was eliminated: after treatment of the glycopeptide with leucine-aminopeptidase, and after mixing it with alpha 1-acid glycoprotein. The glycopeptide did not inhibit cell proliferation in kidney, submaxillary gland, or tongue epithelium. It seems to be the active component of a system that inhibits the proliferation of hepatocytes, probably by reducing their sensitivity to various mitogenic stimuli.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"20 3","pages":"331-41"},"PeriodicalIF":0.0,"publicationDate":"1987-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1987.tb01315.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14809721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Growth in solid heterogeneous human colon adenocarcinomas: comparison of simple logistical models. 实体异质人结肠腺癌的生长:简单逻辑模型的比较。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01316.x

S Michelson, A S Glicksman, J T Leith

{"title":"Growth in solid heterogeneous human colon adenocarcinomas: comparison of simple logistical models.","authors":"S Michelson, A S Glicksman, J T Leith","doi":"10.1111/j.1365-2184.1987.tb01316.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1987.tb01316.x","url":null,"abstract":"Three models of simple logistical growth were used to describe volumetric growth in heterogeneous tumours. Two clonal subpopulations (designated as clone A and clone D) originally obtained from a human colon adenocarcinoma were used to produce solid xenograft tumours in nude mice. Volumetric growth of tumours produced from pure cells alone was compared to that produced from 50% A:50% D, 88% A:12% D, and 9% A:91% D admixtures. Gompertzian analysis of the in vivo growth data indicated significant differences in both the initial growth rates and final asymptotic limiting volumes of the pure versus the admixed tumours. Verhulstian and modified Verhulstian models were also used to derive regression curves from the same data. The fit of the curves was compared with each other using standard (Akaike, 1974; Schwartz, 1978) information criteria. In four of the five tumour populations the Gompertz equation fitted best. Only in the 88% A:12% D tumours did the modified Verhulst model fit best. The deviations from the regression curves, the residuals, for all three models were systematically distributed. These systematic errors are likely to be the result of using simplified logistical models to describe the growth kinetics of interacting populations in heterogeneous tumours.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"20 3","pages":"343-55"},"PeriodicalIF":0.0,"publicationDate":"1987-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1987.tb01316.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14809722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Studies of cell polyploidy in the heart. 心脏细胞多倍体的研究。

Cell and tissue kinetics Pub Date : 1987-05-01

V Y Brodsky

引用次数: 0

Haemopoietic stem cell proliferation in the bone marrow of S1/S1d mice. 造血干细胞在S1/S1d小鼠骨髓中的增殖。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01312.x

E G Wright, S A Lorimore

{"title":"Haemopoietic stem cell proliferation in the bone marrow of S1/S1d mice.","authors":"E G Wright, S A Lorimore","doi":"10.1111/j.1365-2184.1987.tb01312.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1987.tb01312.x","url":null,"abstract":"In marrow from Sl/Sld mice (but not +/+ mice) day 7 and day 8 CFU-S proliferate whilst day 10 and day 12 CFU-S exhibit negligible proliferation. Media conditioned by both +/+ and Sl/Sld marrow contains an inhibitor of CFU-S proliferation but day 8 CFU-S in +/+ and Sl/Sld marrow show marked dose-response differences to this factor. To inhibit the proliferation of Sl/Sld CFU-S required approximately ten times the concentration of inhibitor that inhibited the proliferation of +/+ CFU-S. Thus abnormally responsive day 8-CFU-S were shown to proliferate in an inhibitory environment. Abnormalities in Sl/Sld CFU-S function were also demonstrated in heterotopic transplantation experiments using +/+ and Sl/Sld donors and hosts to obtain ectopic bone marrow with various stromal (donor) and haemopoietic (host) combinations. Day 8 Sl/Sld CFU-S were seen to proliferate, irrespective of whether the stromal environment was derived from Sl/Sld or +/+ marrow. Sl/Sld mice are generally regarded as animals in which there is a genetically determined defect in haemopoiesis due to an abnormality in the haemopoietic environment. It is difficult, however, to attribute the abnormal CFU-S behaviour in these experiments to environmental factors and the results are consistent with mutation at the Sl locus affecting the responses of CFU-S to regulatory signals, i.e. the genetic defect is not confined to the stromal environment.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"20 3","pages":"301-10"},"PeriodicalIF":0.0,"publicationDate":"1987-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1987.tb01312.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14444777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Circadian studies. 生理研究。

Cell and tissue kinetics Pub Date : 1987-01-01

E R Burns

引用次数: 0

Variations in crypt cell cycle time. 隐窝细胞周期时间的变化。

Cell and tissue kinetics Pub Date : 1987-01-01

B Maurer-Schultze, E D Wachsmuth

引用次数: 0

Adrenergic receptors and the control of cell division in the intestinal epithelium. 肠上皮内肾上腺素能受体与细胞分裂的调控。

Cell and tissue kinetics Pub Date : 1986-09-01

P J Tutton

引用次数: 0