Cell and tissue kinetics最新文献_第2页

A technique to analyse the emerging zonality of heterogeneous solid tumours. 一种分析异质实体瘤新出现的地带性的技术。

Cell and tissue kinetics Pub Date : 1987-09-01 DOI: 10.1111/j.1365-2184.1987.tb01359.x

S Michelson, J T Leith, A S Glicksman

{"title":"A technique to analyse the emerging zonality of heterogeneous solid tumours.","authors":"S Michelson, J T Leith, A S Glicksman","doi":"10.1111/j.1365-2184.1987.tb01359.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1987.tb01359.x","url":null,"abstract":"A technique to analyse the time-dependent emergence of homogeneous regions composed of cells from a single clone within an artificial (clonally) heterogeneous tumour is described. Neoplasms were grown in vivo as xenografts made from varying proportions of the dichotomous subpopulations (Clones A and D). They were sampled frequently for volume and composition. A variable number of tumour cross-sections were taken as part of the sampling technique. The random subsamples obtained from each cross-section were enzymatically disaggregated into single cells. From the single cell disaggregates the composition of the tumours was estimated. An estimator for the global proportion of cells was then calculated from all the single cell disaggregates. Time-dependent changes in the overall composition of the tumour requires that a time-dependent estimate of the global proportions of the subpopulations be calculated from each sample. Analysis of the sample proportions results in a statistic which can be tested for goodness-of-fit against a standardized normal variate as a test of emerging zonality. Data from three artificial admixtures were examined. The results show that 'zonality', i.e. regions composed primarily of single subpopulations, emerges in all cases. However, the rate at which the zones emerge appears to depend on the 'compositional stability'. Robustness studies show that the technique is robust with respect to the global estimator of the proportion.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"20 5","pages":"499-506"},"PeriodicalIF":0.0,"publicationDate":"1987-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1987.tb01359.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14574559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A study of cell migration in the adrenal cortex of the rat using bromodeoxyuridine. 溴脱氧尿苷对大鼠肾上腺皮质细胞迁移的研究。

Cell and tissue kinetics Pub Date : 1987-09-01 DOI: 10.1111/j.1365-2184.1987.tb01361.x

A M McNicol, A E Duffy

引用次数: 37

Sensitivity of flow cytometric data to variations in cell cycle parameters. 流式细胞术数据对细胞周期参数变化的敏感性。

Cell and tissue kinetics Pub Date : 1987-09-01 DOI: 10.1111/j.1365-2184.1987.tb01360.x

P Ubezio, A Rossotti

{"title":"Sensitivity of flow cytometric data to variations in cell cycle parameters.","authors":"P Ubezio, A Rossotti","doi":"10.1111/j.1365-2184.1987.tb01360.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1987.tb01360.x","url":null,"abstract":"We investigated to what extent flow cytometric DNA histograms are informative of cell cycle parameters. We created a computer program to simulate cell cycle progression in a generic and flexible way. Various scenarios, characterized by different models and distributions of cell cycle phase transit times, have been analysed in order to obtain the percentages of cells in the different cell cycle phases during exponential growth and their time course after mitotic block. Cell percentages during exponential growth were insensitive to intercell variability in phase transit times and thus can be employed to estimate the relative mean phase transit times, even in the presence of non-cycling cells. However, this information is ambiguous if re-entry of such cells into the cycling status is permitted. The stathmokinetic outline gives the mean phase transit times, but also provides information about the spread, but not the form, of the phase transit time distributions, being particularly sensitive to the spread of G1 phase duration. The stathmokinetic outline also helps distinguish between scenarios considering only cycling cells, those forecasting a fraction of definitively non-cycling cells and those admitting a G0 status with first-order output kinetics.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"20 5","pages":"507-17"},"PeriodicalIF":0.0,"publicationDate":"1987-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1987.tb01360.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14574560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

In vitro effect of a glycopeptide acting in vivo on hepatocyte cell cycle. 一种糖肽在体内对肝细胞周期的体外影响。

Cell and tissue kinetics Pub Date : 1987-09-01 DOI: 10.1111/j.1365-2184.1987.tb01358.x

M N Lombard, C Nadal, M J Monnot

引用次数: 3

Cell cycle responses of heterogeneous human colon adenocarcinoma subpopulations to X-irradiation. 异种人结肠腺癌亚群对x射线照射的细胞周期反应。

Cell and tissue kinetics Pub Date : 1987-09-01 DOI: 10.1111/j.1365-2184.1987.tb01356.x

S F Bliven, T E Schneiderman, J T Leith

引用次数: 8

Measurement of the transit time for cells through the epidermis and stratum corneum of the mouse and guinea-pig. 细胞通过小鼠和豚鼠表皮和角质层传递时间的测定。

Cell and tissue kinetics Pub Date : 1987-09-01 DOI: 10.1111/j.1365-2184.1987.tb01355.x

C S Potten, R Saffhill, H I Maibach

{"title":"Measurement of the transit time for cells through the epidermis and stratum corneum of the mouse and guinea-pig.","authors":"C S Potten, R Saffhill, H I Maibach","doi":"10.1111/j.1365-2184.1987.tb01355.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1987.tb01355.x","url":null,"abstract":"A new approach to determine the transit time through the epidermis is presented, involving a gentle washing of the skin surface to collect the loosely attached surface corneocytes. This, it is believed, will be less likely to stimulate the system than tape-stripping or scraping. Radioactively labelled thymidine and iododeoxyuridine have been used to label cells in the basal layer and various labelled amino acids (glycine, cystine and methionine) have been used to label the metabolically viable cell layers (up to and including the granular layer). The resulting changes in surface radioactivity levels have been interpreted to provide a basal to surface transit time of 8-9.5 days for hairless and haired mouse epidermis and about 13.5 days for guinea-pigs. The basal to granular layer transit time, which probably includes some basal layer residence time, is about 4.5 days in the mouse and 8 days in the guinea-pig. The granular to surface time in mice is about 5 days. The results also suggest that when nuclear and cytoplasmic organelles are degraded in the granular layer, material is released that can diffuse rapidly through the stratum corneum to the surface. Some of this can be shown by chromatography to be thymidine. Hence, the stratum corneum is previous to molecules such as nucleosides. This rapid diffusion outwards through the skin can also be detected shortly after injecting [125I]-iododeoxyuridine.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"20 5","pages":"461-72"},"PeriodicalIF":0.0,"publicationDate":"1987-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1987.tb01355.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14575768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

Proliferation kinetics of plasma cells and of normal haemopoietic cells in multiple myeloma. 多发性骨髓瘤中浆细胞和正常造血细胞的增殖动力学。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01313.x

G Ucci, A Riccardi, P Dörmer, M Danova, R Luoni, C M Montecucco, R Ciotti, M Girino

{"title":"Proliferation kinetics of plasma cells and of normal haemopoietic cells in multiple myeloma.","authors":"G Ucci, A Riccardi, P Dörmer, M Danova, R Luoni, C M Montecucco, R Ciotti, M Girino","doi":"10.1111/j.1365-2184.1987.tb01313.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1987.tb01313.x","url":null,"abstract":"DNA synthesis time (Ts) and 3H thymidine (TdR) labelling index (LI) of bone marrow (BM) myelomatous plasma cells (PC) and of the residual haemopoietic cell population (RHCP) were measured by in vitro quantitative 14C-TdR autoradiography in five patients with multiple myeloma (MM) in different phases of disease (three at presentation and two at relapse) and in one patient with solitary extra-osseous myeloma. One other patient with plasma cell leukaemia (PCL) was studied during an initial relapse phase and later during the leukaemic terminal phase. PC Ts was 18.8 +/- 3.7 (from 13.3 to 25.0) hr and PC LI was 2.5 +/- 1.8% (from 1.0 to 6.3%). In the case of PCL, circulating PC had a Ts of 14.4 hr and a LI of 3.1. From these experimental measurements, the fractional turnover rate (FTR-percentage of cells produced per unit time) and the potential doubling time (Td) of BMPC were calculated assuming that all BMPC were in a steady-state at the time of the study. BMPC FTR was 3.53 +/- 2.3% cells per day (from 1.2 to 6.72) and BMPC Td was 46.8 +/- 27.5 days (from 15.0 to 75.4). Comparison with results obtained in BM blasts of children with acute lymphoblastic leukaemia (ALL) indicated that BMPC had a lower proliferative activity (P less than 0.001), although BMPC Ts was not significantly different. In two patients a tumour doubling time of 6 and 13 months was determined by clinical follow up. Comparison of this parameter with Td showed a cell loss factor of more than 90% in both patients. Kinetic data relative to RHCP showed slight variations with respect to those found in normal subjects, with a general tendency towards a prolongation of Ts and a reduction of LI.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"20 3","pages":"311-8"},"PeriodicalIF":0.0,"publicationDate":"1987-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1987.tb01313.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14604368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Comparison of growth and cell proliferation kinetics during mouse molar odontogenesis in vivo and in vitro. 小鼠磨牙在体内和体外形成过程中生长和细胞增殖动力学比较。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01314.x

N Ahmad, J V Ruch

引用次数: 40

Combined flow and absorption DNA measurements of [3H]TdR-labelled tumour cells. I. Studies of MCa-11 cells grown as tumours in vivo and as exponential cultures in vitro. [3H] tdr标记肿瘤细胞的流动和吸收DNA联合测量。I.体内肿瘤生长的MCa-11细胞和体外指数培养的研究。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01310.x

D C Allison, M Chakerian, P F Ridolpho, S Anderson, S Curley, M E Wilder, J Robertson

{"title":"Combined flow and absorption DNA measurements of [3H]TdR-labelled tumour cells. I. Studies of MCa-11 cells grown as tumours in vivo and as exponential cultures in vitro.","authors":"D C Allison, M Chakerian, P F Ridolpho, S Anderson, S Curley, M E Wilder, J Robertson","doi":"10.1111/j.1365-2184.1987.tb01310.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1987.tb01310.x","url":null,"abstract":"Flow cytometry of cellular DNA content provides rapid estimates of DNA distributions, i.e. the proportions of cells in the different phases of the cell cycle. Measurements of DNA alone, however, yield no kinetic information and can make it difficult to resolve the cell cycle distributions of normal and transformed cells present in tumour biopsy specimens. The use of absorption cytophotometry of the Feulgen DNA content and [3H]TdR labelling of the same nuclei provides objective criteria to distinguish the ranges of DNA content for G0/G1, S, and G2/M cells. We now report on a study in which we combined flow and absorption cytometry to resolve the cell cycle distributions of host and tumour cells present in biopsy specimens of MCa-11 mouse mammary tumours labelled in vivo for 0.5 hr with [3H]TdR. A similar analysis of exponential monolayer cultures, labelled for 5 min with [3H]TdR under pulse-chase conditions, revealed a highly synchronous traversal of almost all cells through the different phases of the cell cycle. Combination of the flow and absorption methods also allowed us to detect G2 tumour cells in vivo and a minor tumour stem-line in vitro, to show that these two techniques are complementary and yield new information when they are combined.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"20 3","pages":"273-90"},"PeriodicalIF":0.0,"publicationDate":"1987-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1987.tb01310.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14809719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers. 骨髓成骨干细胞:体外培养与扩散室移植。

Cell and tissue kinetics Pub Date : 1987-05-01 DOI: 10.1111/j.1365-2184.1987.tb01309.x

A J Friedenstein, R K Chailakhyan, U V Gerasimov

引用次数: 1054