Cell and tissue kinetics最新文献_第10页

Analysis of the variability and of the lengthening of intercleavage times during the cleavage stages of Nothobranchius guentheri. 黄颡鱼(nothobranchus guentheri)卵裂期的变异及卵裂时间延长的分析。

Cell and tissue kinetics Pub Date : 1983-03-01

R Van Haarlem, R Van Wijk, J G Konings

引用次数: 0

Circadian rhythms in phases of the cell cycle in the hamster as demonstrated by flow cytometry. 流式细胞术显示的仓鼠细胞周期阶段的昼夜节律。

Cell and tissue kinetics Pub Date : 1983-03-01

N H Rubin, J A Hokanson, G Bogdon

{"title":"Circadian rhythms in phases of the cell cycle in the hamster as demonstrated by flow cytometry.","authors":"N H Rubin, J A Hokanson, G Bogdon","doi":"","DOIUrl":"","url":null,"abstract":"Circadian rhythmicity in the phases of the cell cycle of several epithelial tissues of the hamster was analysed by flow cytometry. Hamsters were killed every 3 hr for 24 hr to permit observation of the effect of 'time of day' on the proportion of cells in the various phases of the cell cycle. Bone marrow and epithelium from cheek pouch, oesophagus and tongue were isolated and processed to single cell suspensions for analysis. The only systematic difference in the collection of the data was the time of day when the hamsters were killed. From the resulting DNA histograms, derived by flow cytometry (which was chosen as the technique for this study because of demonstrated applicability in determining cellular properties), the G1, S, and G2 fractions were estimated. Multiple linear regression techniques were used to estimate circadian periodicity in the data. The calculated circadian rhythms in tongue and oesophagus, derived by flow cytometry, were consistent with murine rhythms previously reported, as derived by other techniques. Circadian rhythms also were demonstrated by flow cytometry to be present in the cheek pouch epithelium, as has been reported by others. Although there was considerable fluctuation in findings from the bone marrow, the fluctuation was not of circadian periodicity, perhaps because of the mixed cell population. This study validates the reliability of older techniques, such as mitotic indices, labelling indices, and uptake of tritiated thymidine, which show the rhythmic nature of cell division in vivo.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 2","pages":"115-23"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17882799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Renewal of type A spermatogonia in adult rats. 成年大鼠A型精原细胞的更新。

Cell and tissue kinetics Pub Date : 1983-03-01

J Bartmanska, Y Clermont

{"title":"Renewal of type A spermatogonia in adult rats.","authors":"J Bartmanska, Y Clermont","doi":"","DOIUrl":"","url":null,"abstract":"The origin of type A spermatogonia in the rat, formed at each cycle of the seminiferous epithelium, has been investigated in segments of tubules dissected from the testes of animals killed at 1 and 44 hr after a single injection of [3H]thymidine. After fixation and staining these tubular segments were radioautographed according to the technique of Huckins & Kopriwa (1969). Labelled and unlabelled type A0, A4, A1 and intermediate spermatogonia were mapped and scored in tubular segments at stages I-IV of the cycle of the seminiferous epithelium, i.e. before, during and after the stage I peak of spermatogonial mitosis. In stage I of the cycle, the relatively rare type A0 spermatogonia (0.7 per frame) were infrequently labelled at the two time intervals, while the large majority of numerous type A4 spermatogonia (8.7 per frame) were labelled. In stages II-IV of the cycle, of the numerous aligned type A1 spermatogonia, only a few were labelled at 1 hr, whereas many were labelled at 44 hr after [3H]thymidine injection. At the latter time interval, the intermediate spermatogonia were also well labelled. Thus, considering cell numbers and labelling indices, it appears that the type A4 spermatogonia may serve as precursors for both type A1 and intermediate spermatogonia.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 2","pages":"135-43"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17882800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma levels of growth hormone, corticosterone and insulin, during induced hepatocyte synchronization in young rats. 幼年大鼠诱导肝细胞同步过程中血浆生长激素、皮质酮和胰岛素水平的变化。

Cell and tissue kinetics Pub Date : 1983-03-01

M N Lombard, M Rieutort, M T Strosser, B Koch, C Nadal

{"title":"Plasma levels of growth hormone, corticosterone and insulin, during induced hepatocyte synchronization in young rats.","authors":"M N Lombard, M Rieutort, M T Strosser, B Koch, C Nadal","doi":"","DOIUrl":"","url":null,"abstract":"A wave of synchronous hepatocytes entering the cell cycle can be obtained in vivo after a subcutaneous injection (e.g. of casein) in rats at around Post-natal Day 10, when plasma growth hormone (GH) levels reach a low plateau (40 +/- 2 ng/ml) and liver cell proliferation rate is high. The present work reports the following changes in plasma hormone concentrations after synchronization of 20% of the hepatocyte population: (1) during the G1 phase (i.e. 6-12 hr after the mitogenic trigger), plasma GH concentration has dropped further (25 +/- 1.5 ng/ml). It was back to 90% of control levels during the S phase, mitosis and the following response including a transitory decrease in labelling index below control values. Injected together with the irritating mitotic trigger, a single dose of rat GH reduced the cell synchronization and post-synchronization effects by 50%. (2) Plasma corticosterone levels varied inversely to those of GH, increasing to twice the control values during G1 and were back to physiological levels when synchronized hepatocytes entered the S phase. (3) Variations in insulin levels were similar to that of corticosterone, with narrower ranges and reduced amplitudes. Our data suggest a possible correlation between the observed variations in plasma hormone levels and the induced synchronous hepatocyte response.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 2","pages":"145-53"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17399917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the relationship between the variation in intercleavage times and cell diversification during the cleavage stages of the teleost fish Nothobranchius guentheri. 硬骨鱼(nothobranchus guentheri)卵裂期卵裂时间变化与细胞分化的关系分析。

Cell and tissue kinetics Pub Date : 1983-03-01

R Van Haarlem, J G Konings, R Van Wijk

引用次数: 0

Spermatogonial multiplication in the Chinese hamster. II. Cell cycle properties of undifferentiated spermatogonia. 中国仓鼠的精原繁殖。2未分化精原细胞的细胞周期特性。

Cell and tissue kinetics Pub Date : 1983-01-01

D Lok, M T Jansen, D G de Rooij

{"title":"Spermatogonial multiplication in the Chinese hamster. II. Cell cycle properties of undifferentiated spermatogonia.","authors":"D Lok, M T Jansen, D G de Rooij","doi":"","DOIUrl":"","url":null,"abstract":"The cell cycle properties of undifferentiated spermatogonia in the Chinese hamster were analysed by the fraction of labelled mitoses technique (FLM) in whole mounted seminiferous tubules. The minimum cell cycle time (Tc) was found to be c. 90 hr for the As and 87 hr for the Apr and Aal spermatogonia, which is appreciably longer than for the differentiating types A2-B2 spermatogonia (60 hr). This is mainly accounted for by a longer tG1. In general the variability in the duration of the cell cycle phases is greater than for differentiating spermatogonia. From the shape and position of the second peak of the FLM curve it could be concluded that the undifferentiated spermatogonia either cycle with a Tc of c. 87-90 hr, or become arrested in G1. This implies that the decrease in proliferative activity of the undifferentiated spermatogonia after stage IV takes place by the arrest of progressively more cells, i.e. by a gradual decrease of the growth fraction, and not by a gradual lengthening of tG1. The arrested cells either differentiate into A1 spermatogonia and divide in stage IX, or remain undifferentiated and are stimulated to enter S again during the following epithelial cycle. It could be deduced from the heights of the second FLM peaks of As and Apr spermatogonia that once triggered into active cycle, the daughter cells of As spermatogonia that became Apr have a greater chance to continue cycling than those that became new As cells.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 1","pages":"19-29"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17877202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cell kinetic method for the mitotic selection of treated G2 cells. 处理G2细胞有丝分裂选择的细胞动力学方法。

Cell and tissue kinetics Pub Date : 1983-01-01

M H Schneiderman, G S Schneiderman, C M Rusk

{"title":"A cell kinetic method for the mitotic selection of treated G2 cells.","authors":"M H Schneiderman, G S Schneiderman, C M Rusk","doi":"","DOIUrl":"","url":null,"abstract":"G2 cells treated with 150 rad X-radiation were isolated from a monolayer culture of exponentially growing Chinese hamster ovary (CHO) cells by a combination of 125Iododeoxyuridine ([125I]UdR)-induced blockade of S-phase cell progression, treatment and mitotic selection (125I-TMS technique). Once the rate at which cells were selected from a small window in mitosis was established (Schneiderman et al., 1972), the cells were exposed to 10 microCi/ml, carrier-free [125I]UdR for 10 min immediately before treatment with 150 rads X-radiation. After X-irradiation the cells located later in the cell cycle than the X-ray-induced division delay transition point (TPx), at or just prior to prophase, progressed without delay and were selected during the next 50 min (Walters & Petersen, 1968; Schneiderman et al., 1972). The G2- and S-phase cells, located prior to the TPx, sustained a transitory delay and resumed progression into mitosis only after recovery from the radiation insult (Terasima & Tolmach, 1963). However, S-phase cells having incorporated [125I]UdR during the pulse label were prevented from entering mitosis (Schneiderman & Hofer, 1980) and only the X-ray-treated G2 cells resumed progression into mitosis and were selected.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 1","pages":"41-9"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17877203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison of the use of bromodeoxyuridine and [3H]thymidine in studies of the cell cycle. 溴脱氧尿嘧啶与[3H]胸苷在细胞周期研究中的比较。

Cell and tissue kinetics Pub Date : 1983-01-01

A H Cawood, J R Savage

{"title":"A comparison of the use of bromodeoxyuridine and [3H]thymidine in studies of the cell cycle.","authors":"A H Cawood, J R Savage","doi":"","DOIUrl":"","url":null,"abstract":"Untransformed Syrian hamster fibroblasts in short-term culture were studied by means of [3H]thymidine autoradiography combined with differential staining after continuous exposure to bromodeoxyuridine. Fractions of 3H-labelled metaphases (FLM) and of differentially stained metaphases (FDM) were determined in sequential samples. The resulting FLM and FDM curves are in close agreement, in sequential samples. The resulting FLM and FDM curves are in close agreement, except at later sample times (10 hr onwards), when the FLM is greater than the FDM. This inefficiency on the part of the bromodeoxyuridine technique in the detection of cells in early S-phase leads to an underestimate of the average duration of S compared with that derived from autoradiography. Grain counts suggest that only a small proportion of total DNA synthesis is missed. FLM and FDM curves from both diploid and tetraploid cells are very similar. Despite the inefficiency in the detection of cells in early S phase, the bromodeoxyuridine technique has a number of useful features in studies of the cell cycle. It is possible to distinguish pre-S cells from G2 cells unambiguously, and cells in their first cycle can be distinguished from those in their second with a much greater efficiency than by autoradiography. The technique is consistent and has no problems from background or the consequent need for thresholds in scoring. As is demonstrated here, the two methods can be combined, the differential staining being little affected by the autoradiographic procedures.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 1","pages":"51-7"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17877204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erythropoietin-dependent and erythropoietin-independent enhancement of colony formation by immature erythroid progenitors (BFUe). 促红细胞生成素依赖性和非依赖性增强未成熟红细胞祖细胞(BFUe)集落形成。

Cell and tissue kinetics Pub Date : 1983-01-01

J F Eliason, G Van Zant

{"title":"Erythropoietin-dependent and erythropoietin-independent enhancement of colony formation by immature erythroid progenitors (BFUe).","authors":"J F Eliason, G Van Zant","doi":"","DOIUrl":"","url":null,"abstract":"The erythropoietin (epo)-dependent burst forming activities (BFA) of foetal calf serum (FCS), mouse bone marrow cells (BFA-cell) and lectin-stimulated mouse spleen conditioned medium (BFA-mscm) were investigated. Burst enhancement by BFA-mscm was independent of FCS concentration above 5% FCS. The activity of BFA-cell was directly proportional to FCS concentration. There was a high correlation for the relationship between the logarithm of total cell concentration and the logarithm of burst number for each concentration of FCS. Burst formation in the presence of BFA-cell showed a high epo requirement whereas plateau numbers of bursts were evident at concentrations of 1 to 2 units of epo/ml in cultures containing BFA-mscm. The epo-independent developmental activities (EIDA) of BFA-cell and BFA-mscm were examined in methylcellulose cultures to which the addition of epo was delayed for up to four days. Under all conditions examined, the number of bursts formed in the presence of BFA-cell declined with increasing delay of epo addition. Studies on the kinetics of haemoglobin synthesis in such experiments demonstrated that this decline in colony number was associated with a delay in haemoglobin synthesis. Burst formation in cultures containing various levels of BFA-mscm was independent of the time of epo addition. These results indicate that the burst enhancing ability of BFA-mscm is directly related to its EIDA whereas bone marrow cells appear to lack this activity.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 1","pages":"65-76"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17877206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective maintenance of cultured epithelial cells from DMBA-induced mammary tumours by bovine colostrum supplement. 补充牛初乳对dmba诱导乳腺肿瘤上皮细胞的选择性维持作用。

Cell and tissue kinetics Pub Date : 1983-01-01

M T Tseng, A R Safa, R Ballou

引用次数: 0