Cell and tissue kinetics最新文献_第9页

Cytophotometric measurement of the cellular DNA content of [3H]thymidine-labelled spheroids. Demonstration that some non-labelled cells have S and G2 DNA content. [3H]胸腺嘧啶标记球体细胞DNA含量的细胞光度测定。证明一些未标记的细胞有S和G2 DNA含量。

Cell and tissue kinetics Pub Date : 1983-05-01

D C Allison, J M Yuhas, P F Ridolpho, S L Anderson, T S Johnson

引用次数: 0

The stimulatory effect of serum thymic factor (FTS) on spontaneous DNA synthesis of mouse thymocytes. 血清胸腺因子(FTS)对小鼠胸腺细胞自发DNA合成的刺激作用。

Cell and tissue kinetics Pub Date : 1983-05-01

I Blazsek, M Lenfant

引用次数: 0

Inhibition of cells in culture by polyamines does not depend on the presence of ruminant serum. 多胺对培养细胞的抑制作用与反刍动物血清的存在无关。

Cell and tissue kinetics Pub Date : 1983-05-01

C J Smith, R Maschler, H R Maurer, J C Allen

引用次数: 0

Blocking of the cell division by stress-inducing electrical stimulation. A study in rat oral epithelium. 通过压力诱导的电刺激来阻止细胞分裂。大鼠口腔上皮的研究。

Cell and tissue kinetics Pub Date : 1983-05-01

A V Fisker, E Ostergaard, T Karring

{"title":"Blocking of the cell division by stress-inducing electrical stimulation. A study in rat oral epithelium.","authors":"A V Fisker, E Ostergaard, T Karring","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of the present study was to localize in the cell cycle, the site of the stress-induced blockage of cells entering the mitotic phase, and to estimate the length of time this block is effective. A total of 140 rats were subjected to electrical stimulation applied by a live metal grill in the bottom of their cages. Forty animals left undisturbed in the cages were used as controls. At various intervals after the start of electrical stimulation, groups of animals were killed and histologic sections were prepared of the palatal mucosa. The number of prophases, metaphases, and ana/telophases was counted in the epithelium in three sections of each animal. Electrical stimulation for 1 min resulted in a blocking of the entrance of cells into mitosis, followed by a transient increase in the number of mitotic figures to a level much higher than that of the controls. Electrical stimulation for 10 min resulted in the maintenance of the blocking effect for approximately 45 min. By renewed electrical stimulation the period of blockage was extended for a further 35 min. In each experiment the number of prophases decreased immediately after the start of electrical stimulation, indicating that the site of the blockage of the entrance of cells into mitosis is located near the G2/M transition.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 3","pages":"259-67"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17890182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The action of 5-amino uracil on log growth and division-synchronized Tetrahymena. 5-氨基尿嘧啶对四膜虫生长和分裂同步的作用。

Cell and tissue kinetics Pub Date : 1983-05-01

J E Thomas, A M Zimmerman

{"title":"The action of 5-amino uracil on log growth and division-synchronized Tetrahymena.","authors":"J E Thomas, A M Zimmerman","doi":"","DOIUrl":"","url":null,"abstract":"The influence of 5-amino uracil (5-AU) was investigated on the cell cycle of log growth and division-synchronized Tetrahymena pyriformis GL. The division index of log growth phase Tetrahymena was suppressed by 50% after 40 min in 8 mM 5-AU. Cells division-synthronized by one heat shock per generation were also treated with 5-AU. Cells treated either prior to the first synchronous division (80 min EH) or up to 25 min prior to the second synchronous division (after 160 min EH) were not delayed in their progress through the cell cycle. Cells treated during the S phase of the first free running cell cycle, however, were delayed 5-30 min from reaching the second synchronous division. The effect of 5-AU on DNA and RNA synthesis was also examined. Incorporation of [3H]thymidine into acid-precipitable material was reduced in the presence of 5-AU; the rate of DNA synthesis was also reduced. The depression in the rate of DNA synthesis was greater at the beginning of S than at the end of S. The size of the thymidine pool (nucleosides + nucleotides) did not change during 5-AU treatment; however, an accumulation of thymidine tri-phosphate and a decrease in the amount of thymidine nucleoside was observed. A suppression of [14C]uridine incorporation resulting from 5-AU treatment was observed throughout the cell cycle. The rate of RNA synthesis as monitored by [14C]uridine incorporation into acid precipitable material was also reduced during 5-AU treatment. No change in either the size or the composition of the pool of uridine (nucleoside + nucleotide) was detected in 5-AU treated cells as compared to controls.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 3","pages":"285-301"},"PeriodicalIF":0.0,"publicationDate":"1983-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17253950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell dynamics in explants derived from tracheas of hamsters fed normal and vitamin A-deficient diets. 饲养正常和缺乏维生素a的仓鼠气管外植体的细胞动力学。