表皮组织稳态。完整蟾蜍,Bufo Bufo的细胞池大小,细胞出生率和细胞脱落。

Cell and tissue kinetics Pub Date : 1985-09-01
P E Budtz
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引用次数: 0

摘要

蟾蜍表皮是研究组织稳态的合适模型,因为可以很容易地确定细胞池的大小、细胞池的流入和流出。细胞池大小通过显微照片上的细胞计数获得,内流(细胞出生率)通过中期阻滞技术评估,流出(细胞脱落损失)通过计算角膜层细胞数量和记录脱落间隔期来评估。强调了评估每平方单位皮肤表面这些参数的方法的重要性。这些参数在8组10只成年雄蟾蜍中进行了研究,这些雄蟾蜍在一天的不同时间被处死。细胞出生率有微小的变化,在平均26个细胞/mm2/小时上下波动(在中期收集期11:00 - 14:00小时获得)。通过对8个3小时中期收集期的细胞产量进行汇总,并外推到换毛间隔期(两次换毛之间的时间),计算得出10.3天内细胞产量约为6340个细胞/mm2,而每次换毛的细胞损失仅为2370个细胞/mm2。因此,细胞生成率比脱毛导致的细胞损失率高出2.7倍。有人提出,产生的“过剩”细胞不能永久地容纳在活表皮内。因此,可以计算出在10.3天内脱毛约4000个细胞/mm2或16个细胞/mm2/hr的细胞缺失率。这些结果讨论了有关当前概念的组织稳态机制(s)。结果与受控细胞缺失可能是一种组织稳态机制的假设是一致的,补充受控细胞分裂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epidermal tissue homeostasis. I. Cell pool size, cell birth rate and cell loss by moulting in the intact toad, Bufo bufo.

Toad epidermis is a suitable model for studies on tissue homeostasis because cell pool size, influx into and efflux from the cell pool can be easily determined. The cell pool size was obtained by cell counting on photomicrographs, the influx (cell birth rate) was assessed by the metaphase-arrest technique, and the efflux (cell loss by moulting) assessed by counting the number of cells in the corneal layer and recording of intermoult periods. The importance of the methods for assessing these parameters per square unit of skin surface is emphasized. These parameters were studied in eight groups of ten adult male toads sacrificed at various hours of the day. There were minor variations in the cell birth rate, fluctuating around a mean of 26 cells/mm2/hr (obtained at the metaphase collection period from 11.00-14.00 hours). By summation of the cell productions during the eight metaphase collection periods of 3 hr, and extrapolation to an intermoult period (time between two moults), a calculated cell production of about 6340 cells/mm2 in 10.3 days was obtained, whereas the cell loss at each moult was only 2370 cells/mm2. Thus the cell production rate exceeds the rate of cell loss through moults by a factor of 2.7 Arguments are presented that the 'surplus' of cells produced cannot be permanently accommodated within the living epidermis. Consequently a cell deletion rate beyond that by moulting of about 4000 cells/mm2 in 10.3 days or 16 cells/mm2/hr can be calculated. These results are discussed in relation to current concepts of tissue homeostatic mechanism(s). The results are consistent with the hypothesis that controlled cell deletion may be a tissue homeostatic mechanism complementary to controlled cell divisions.

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