Cell and tissue kinetics最新文献_第5页

Differential calcium requirements for growth of mouse skin epithelial and fibroblast cells. 小鼠皮肤上皮细胞和成纤维细胞生长对钙的不同需求。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00611.x

M F Kulesz-Martin, D Fabian, J S Bertram

引用次数: 28

Liver cell hyperplasia: on the suitability of using the metaphase-arrest technique. 肝细胞增生:中期阻滞技术应用的适用性。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00610.x

J A Alabi, M R Alison

引用次数: 0

Aspects of statistics in studies of cell proliferation. I. Multistage sampling. 细胞增殖研究中的统计学方面。1、多级采样。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00613.x

D R Appleton

引用次数: 2

The metaphase-arrest technique applied to human cervical epithelium. I. Technique and dose-response studies. 中期阻滞技术在人宫颈上皮中的应用。1 .技术和剂量反应研究。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00609.x

D Ireland, J M Monaghan

引用次数: 1

Mirex-induced adaptive liver growth: a corticosterone-mediated response. mirex诱导的适应性肝脏生长:皮质酮介导的反应。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00605.x

J D Yarbrough, L D Brown, J M Grimley

引用次数: 14

Progressive increase in polyamine levels in 9L cells in vitro during the cell cycle: comparison between cells isolated by centrifugal elutriation and cells grown in synchrony. 体外9L细胞在细胞周期中多胺水平的逐渐增加:离心洗脱分离的细胞与同步生长的细胞的比较。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00602.x

S M Oredsson, J W Gray, L J Marton

{"title":"Progressive increase in polyamine levels in 9L cells in vitro during the cell cycle: comparison between cells isolated by centrifugal elutriation and cells grown in synchrony.","authors":"S M Oredsson, J W Gray, L J Marton","doi":"10.1111/j.1365-2184.1984.tb00602.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1984.tb00602.x","url":null,"abstract":"Centrifugal elutriation was used to separate 9L rat brain tumour cells into fractions enriched in the G1, S, or G2/M phases of the cell cycle. Cells enriched in early G1 phase were recultured, grown in synchrony, and harvested periodically for analysis of their DNA distribution and polyamine content. Mathematical analysis of the DNA distributions indicated that excellent synchrony was obtained with low dispersion throughout the cell cycle. Polyamine accumulation began at the time of seeding, and intracellular levels of putrescine, spermidine, and spermine increased continuously during the cell cycle. In cells in the G2/M phase of the cell cycle, putrescine and spermidine levels were twice as high as in cells in the G1 phase. DNA distribution and polyamine levels were also analysed in cells taken directly from the various elutriation fractions enriched in G1, S, or G2/M. Because we did not obtain pure S or G2/M populations by elutriation or by harvesting synchronized cells, a mathematical procedure--which assumed that the measured polyamine levels for any population were linearly related to the fraction of cells in the G1, S, and G2/M phases times the polyamine levels in these phases and that polyamine levels did not vary within these phases--was used to estimate 'true' phase-specific polyamine levels (levels to be expected if perfect synchrony were achieved). Estimated 'true' phase-specific polyamine levels calculated from the data obtained from cells either sorted by elutriation or obtained from synchronously growing cultures were very similar.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"17 5","pages":"437-44"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1984.tb00602.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17525366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Changes in the DNA labelling index after beta irradiation of the mouse epidermis. 小鼠表皮辐照后DNA标记指数的变化。

Cell and tissue kinetics Pub Date : 1984-09-01 DOI: 10.1111/j.1365-2184.1984.tb00606.x

L S Hansen, J E Coggle, J Wells, M W Charles

{"title":"Changes in the DNA labelling index after beta irradiation of the mouse epidermis.","authors":"L S Hansen, J E Coggle, J Wells, M W Charles","doi":"10.1111/j.1365-2184.1984.tb00606.x","DOIUrl":"https://doi.org/10.1111/j.1365-2184.1984.tb00606.x","url":null,"abstract":"This study looked at the changes in the interfollicular DNA labelling index (LI) with time after strontium-90/yttrium-90 beta irradiation of approximately 100 mm2 of mouse flank skin, after a dose of 100 Gy which produces transitory moist desquamation. Within 24 hr of such a dose the LI of the irradiated area was essentially zero (0.07 +/- 0.03%), whilst those of the side area and of the control area were 15.0 +/- 2.6% and 21.4 +/- 2.7%, respectively. The LI of the side and the control areas then fell within 3-5 days to approximately 4% and approximately 2% respectively, whilst that of the irradiated area rose rapidly to a peak value of 30.2 +/- 1.7% at 10 days post-irradiation. There was a 20% reduction in the diameter of the area with detectable radiation damage within 5 days, and this is primarily due to cell proliferation and migration from the unirradiated margins of the field. In contrast, between days 10 and 20 the major source of repopulation is probably derived from local migration and proliferation of surviving hair follicle basal cells within the irradiated field.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"17 5","pages":"475-81"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2184.1984.tb00606.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17526931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can nocodazole, an inhibitor of microtubule formation, be used to synchronize mammalian cells? Accumulation of cells in mitosis studied by two parametric flow cytometry using acridine orange and by DNA distribution analysis. nocodazole是一种微管形成抑制剂，可以用于同步哺乳动物细胞吗?用吖啶橙双参数流式细胞术和DNA分布分析研究了有丝分裂过程中细胞的积累。

Cell and tissue kinetics Pub Date : 1984-01-01

M Nüsse, H J Egner

{"title":"Can nocodazole, an inhibitor of microtubule formation, be used to synchronize mammalian cells? Accumulation of cells in mitosis studied by two parametric flow cytometry using acridine orange and by DNA distribution analysis.","authors":"M Nüsse, H J Egner","doi":"","DOIUrl":"","url":null,"abstract":"Nocodazole, a temporary inhibitor of microtubule formation, has been used to partly synchronize Ehrlich ascites tumour cells growing in suspension. The gradual entry of cells into mitosis and into the next cell cycle without division during drug treatment has been studied by flow cytometric determination of mitotic cells, analysing red and green fluorescence after low pH treatment and acridine orange staining. Determination of the mitotic index (MI) by this method has been combined with DNA distribution analysis to measure cell-cycle phase durations in asynchronous populations growing in the presence of the drug. With synchronized cells, it was shown that in the concentration range 0.4-4.0 micrograms/l, cells could only be arrested in mitosis for about 7 hr and at 0.04 microgram/ml, for about 5 hr. After these time intervals, the DNA content in nocodazole-blocked cells was found to be increased, and, in parallel, the ratio of red and green fluorescence was found to have changed, showing entry of cells into a next cell cycle without division (polyploidization). It was therefore only possible to partially synchronize an asynchronous population by nocodazole. However, a presynchronized population, e.g. selected G1 cells or metabolically blocked G1/S cells, were readily and without harmful effect resynchronized in M phase by a short treatment (0.4 microgram/ml, 3-4 hr) with nocodazole; after removal of the drug, cells divided and progressed in a highly synchronized fashion through the next cell cycle.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"17 1","pages":"13-23"},"PeriodicalIF":0.0,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17746698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proliferation kinetics of rabbit articular chondrocytes in primary culture and at the first passage. 兔关节软骨细胞原代培养和第一代增殖动力学。

Cell and tissue kinetics Pub Date : 1983-11-01

X Ronot, C Hecquet, P Jaffray, M Guiguet, M Adolphe, J Fontagne, P Lechat

{"title":"Proliferation kinetics of rabbit articular chondrocytes in primary culture and at the first passage.","authors":"X Ronot, C Hecquet, P Jaffray, M Guiguet, M Adolphe, J Fontagne, P Lechat","doi":"","DOIUrl":"","url":null,"abstract":"The in-vitro proliferation kinetics of young rabbit articular chondrocytes were compared in primary culture and at the first passage. The growth curves labelling and mitotic indices, percentage labelled mitosis (PLM) curves and DNA content distributions by flow-microfluorometric analysis during a 7-day growth period were determined in both cases. The length of the cell cycle and the doubling time calculated from the exponential part of the growth curve were quite similar: Tc = 19 hr and Td = 20 hr for the primary culture, Tc = 17 X 3 hr and Td = 20 hr for the first passage. However, the growth curve and the DNA distribution during the 7-day period showed some differences. The duration of the lag period studied by the growth curve was longer in the primary culture than at the first passage. This phenomenon was also observed using the FCM analysis. The growth fraction determination on the second day of culture was in accordance with the lower proliferation capacity of the cells in primary culture. These data suggest that it would be better to study growth kinetics and drug modifications in articular chondrocytes at the first passage than in primary culture.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 6","pages":"531-7"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17683294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell loss from viable and necrotic tumour regions measured by 125I-UdR. 125I-UdR测量存活和坏死肿瘤区域的细胞损失。

Cell and tissue kinetics Pub Date : 1983-11-01

R Porschen, W Porschen, H Mühlensiepen, L E Feinendegen

{"title":"Cell loss from viable and necrotic tumour regions measured by 125I-UdR.","authors":"R Porschen, W Porschen, H Mühlensiepen, L E Feinendegen","doi":"","DOIUrl":"","url":null,"abstract":"Loss of cells from vital and necrotic areas of the syngeneic mammary adenocarcinoma EO 771 in male C57 BL/6J mice may be measured by use of 125I-labelled 5-iodo-2'-deoxyuridine (125I-UdR). Later than 50 hr after an intraperitoneal injection of 20 muCi 125I-UdR the incorporated activity of the entire tumour was externally measured and found to decrease with time after injection. The injected amount was neither chemo- nor radiotoxic. By injecting the vital dye 'light green', unstained necrotic and stained viable regions were separately excised and measured for loss of activity throughout the natural development of the labelled tumour. With the appearance of necrotic regions, labelled viable cells became necrotic, and activity was slowly eliminated. With increasing proportions of necrosis during tumour growth, the rate of loss of activity of the whole tumour decreased. Loss of activity from viable tumour regions reflected cell death and exceeded the loss rates of the whole tumour by a factor of 2 to 3. The data show that loss of activity from the whole tumour results from a superposition of different elimination rates of viable and necrotic tumour regions and is not an immediate consequence of cell death in the course of undisturbed tumour development.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 6","pages":"549-56"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17683296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0